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JP2005348743A Esterase free enzymes 审中-公开
标题翻译：无酵素酶
公开(公告)号：JP2005348743A
公开(公告)日：2005-12-22
申请号：JP2005220051
申请日：2005-07-29
申请人： Sandoz Ag , サンド・アクチエンゲゼルシヤフト
发明人： REICHERT ARNO , RIETHORST WAANDER , KNAUSEDER FRANZ , PALMA NORBERT
IPC分类号： C12N9/98 , C12N9/06 , C12N9/80 , C12N11/00 , C12N11/04 , C12P35/00
CPC分类号： C12N9/0024 , C12N9/80 , C12N11/00 , C12N11/04 , C12P35/00
摘要： PROBLEM TO BE SOLVED: To provide a catalytic process for enzymatically producing 7-aminocephalosporanic acid (7-ACA) in the absence of esterase activity. SOLUTION: Disclosed is a process for decreasing esterase activity present in the presence of D-amino acid oxidase activity or in the presence of glutarylacylase activity in a mixture having esterase activity and D-amino acid oxidase activity and/or having esterase activity and glutarylacylase activity by treatment with phenylmethylsulphonyl fluoride. Immobilized biocatalyts in spherical particle form can be obtained by treating microorganism cells having enzyme activity with a primary or secondary amine containing polymer, an organic solvent which is able to form a two-phase system with water and a bifunctional agent. The process and biocatalysts are used in the production of 7-ACA, 7-amino-3-hydroxymethyl-3-cephem-4-carboxylic acid and cephalosporin antibiotics. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供在没有酯酶活性的情况下酶促产生7-氨基头孢烷酸（7-ACA）的催化方法。解决方案：公开了在具有酯酶活性和D-氨基酸氧化酶活性和/或具有酯酶活性的混合物中，在D-氨基酸氧化酶活性存在下或存在戊二酰化酶活性的情况下，降低酯酶活性的方法和苯甲基磺酰氟处理后的戊二酰化酶活性。具有球形颗粒形式的固定化生物催化剂可以通过用含伯或仲胺聚合物处理具有酶活性的微生物细胞，能够与水和双官能剂形成两相体系的有机溶剂来获得。该方法和生物催化剂用于制备7-ACA，7-氨基-3-羟甲基-3-头孢烯-4-羧酸和头孢菌素抗生素。版权所有（C）2006，JPO＆NCIPI

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