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1. 发明专利

JP2008298785A Compositions and methods for immobilizing nucleic acids to solid supports 审中-公开
标题翻译：将核酸固定化成固体支持物的组合物和方法
公开(公告)号：JP2008298785A
公开(公告)日：2008-12-11
申请号：JP2008152532
申请日：2008-06-11
申请人： Sequenom Inc , シークエノム・インコーポレーテツド
发明人： LOUGH DAVID M , KOESTER HUBERT
IPC分类号： G01N33/53 , C12N15/09 , G01N33/543 , G01N33/545 , G01N33/548 , G01N33/552
摘要： PROBLEM TO BE SOLVED: To provide a composition and manufacturing method of the composition concerned containing at least a bead conjugated to a solid support and also at least conjugated to a macro molecule (for example, nucleic acid). SOLUTION: The obtained surfaces formed from beads linked to a solid support provides favorably increased surface area, increased compared with a "flat" surface, for immobilizing biological particle and macro molecule (especially nucleic acid). Moreover, by selecting a bead with desired functionality, chemical properties of suitable functionalization differing from chemical properties of the solid support can be acquired for immobilizing the selected macro molecule or biological support. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供所述组合物的组合物和制备方法，其至少包含与固体支持物缀合的珠粒，并且至少与大分子（例如，核酸）缀合。解决方案：固定生物颗粒和大分子（特别是核酸），与固体支持物连接的珠形成的所得表面与“平坦”表面相比提高了表面积。此外，通过选择具有所需功能的珠粒，可以获得与固体支持物的化学性质不同的合适的官能化学的化学性质来固定所选择的大分子或生物载体。版权所有（C）2009，JPO＆INPIT

2. 发明授权

US6423966B2 Method and apparatus for maldi analysis 有权
公开(公告)号：US6423966B2
公开(公告)日：2002-07-23
申请号：US48362900
申请日：2000-01-14
申请人： SEQUENOM INC
发明人： HILLENKAMP FRANZ , LOUGH DAVID M , HIGGINS G SCOTT , REUTER DIRK
IPC分类号： G01N27/62 , B01D59/44 , C40B60/14 , G01N27/64 , H01J49/00 , H01J49/04 , H01J49/10 , H01J49/16
CPC分类号： H01J49/164 , B01J2219/00387 , C40B60/14 , H01J49/0418
摘要： Matrix assisted laser desorption/ionization is performed,in a manner to thermalize large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocity and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus "thermalized" the analyte ions are passed to a mass analysis instrument. In a preferred embodiment an optical fiber butts up against a thin transparent plate on which the specimen resides, with the matrix side in a vacuum acceleration chamber. A mechanical stage moves the specimen in both the x- and y-directions to select a point on the specimen which is to receive the radiation. The use of a fiber optic illuminator allows the entire stage assembly to be subsumed essentially within the dimensions of a conventional stage. In other embodiments, a thermalizing compartment is provided in a capillary tube about the end of the illumination fiber and the sample matrix is deposited along the inner cylindrical wall of the tube, so the capillary forms a migration path to the outlet for thermalization of the desorbed analyte. In other embodiments microstructures having the shape of a small lean-to, overhang or perforated cover plate, or providing a high aspect surface texture, provide the necessary containment to promote thermalization of the released analyte. A thin layer or cover of fibrous or permeable material may also be used to thermalize the analyte before mass analysis, and in other embodiment this material may also act as the substrate. An automated instrument may include a fixed array of illumination fibers which are illuminated at different times to eject samples from a corresponding array of points on the specimen.

3. 发明专利

AU2002300406B2 Compositions and Methods for Immobilizing Nucleic Acids to Solid Supports 未知
公开(公告)号：AU2002300406B2
公开(公告)日：2005-05-12
申请号：AU2002300406
申请日：2002-08-02
申请人： SEQUENOM INC
发明人： KOSTER HUBERT , LOUGH DAVID M
IPC分类号： C07H021/00

4. 发明专利

CA2237946C METHOD AND APPARATUS FOR MALDI ANALYSIS 未知
公开(公告)号：CA2237946C
公开(公告)日：2003-06-17
申请号：CA2237946
申请日：1997-09-19
申请人： SEQUENOM INC
发明人： LOUGH DAVID M , HIGGINS SCOTT , REUTER DIRK , HILLENKAMP FRANZ
IPC分类号： G01N27/62 , B01D59/44 , C40B60/14 , G01N27/64 , H01J49/00 , H01J49/04 , H01J49/10 , H01J49/16
摘要： Matrix assisted laser desorption/ionization is performed in a manner to thermalize large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocit y and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus "thermalized", the analyte ions are passed to a mass analysis instrument. In a preferred embodiment, an optical fiber butts up against a thin transparent plate on which the specimen resides, with the matrix side in a vacuum acceleration chamber. A mechanical stage moves the specimen in both the x- a nd y-directions to select a point on the specimen which is to receive the radiation. The use of a fiber optic illuminator allows the entire stage assembly to be subsumed essentially within the dimensions of a conventional stage.

5. 发明专利

AU746737B2 Compositions and methods for immobilizing nucleic acids to solid supports 未知
公开(公告)号：AU746737B2
公开(公告)日：2002-05-02
申请号：AU5247298
申请日：1997-11-06
申请人： SEQUENOM INC
发明人： LOUGH DAVID M , KOSTER HUBERT
IPC分类号： G01N37/00 , B01J19/00 , B01L3/02 , C07B61/00 , C07F9/24 , C07H21/00 , C07H21/02 , C07H21/04 , C07K17/00 , C12M1/00 , C12N15/09 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/53 , G01N35/00 , G01N35/10

6. 发明专利

AT204290T 未知
公开(公告)号：AT204290T
公开(公告)日：2001-09-15
申请号：AT97947374
申请日：1997-11-06
申请人： SEQUENOM INC
发明人： LOUGH DAVID M , KOESTER HUBERT
IPC分类号： G01N37/00 , B01J19/00 , B01L3/02 , C07B61/00 , C07F9/24 , C07H21/00 , C07H21/02 , C07H21/04 , C07K17/00 , C12M1/00 , C12N15/09 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/53 , G01N35/00 , G01N35/10

7. 发明专利

AU724884B2 Method and apparatus for maldi analysis 未知
公开(公告)号：AU724884B2
公开(公告)日：2000-10-05
申请号：AU4342397
申请日：1997-09-19
申请人： SEQUENOM INC
发明人： HILLENKAMP FRANZ , HIGGINS SCOTT , LOUGH DAVID M , REUTER DIRK
IPC分类号： G01N27/62 , B01D59/44 , C40B60/14 , G01N27/64 , H01J49/00 , H01J49/04 , H01J49/10 , H01J49/16
摘要： Matrix assisted laser desorption/ionization is performed in a manner to thermalize large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocity and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus "thermalized" the analyte ions are passed to a mass analysis instrument. In a preferred embodiment an optical fiber butts up against a thin transparent plate on which the specimen resides, with the matrix side in a vacuum acceleration chamber. A mechanical stage moves the specimen in both the x- and y- directions to select a point on the specimen which is to receive the radiation. The use of a fiber optic illuminator allows the entire stage assembly to be subsumed essentially within the dimensions of a conventional stage. In other embodiments, a thermalizing compartment is provided in a capillary tube about the end of the illumination fiber and the sample matrix is deposited along the inner cylindrical wall of the tube, so the capillary forms a migration path to the outlet for thermalization of the desorbed analyte. In other embodiments microstructures having the shape of a small lean-to, overhang or perforated cover plate, or providing a high aspect surface texture, provide the necessary containment to promote thermalization of the released analyte. A thin layer or cover of fibrous or permeable material may also be used to thermalize the analyte before mass analysis, and in another embodiment this material may also act as the substrate. An automated instrument may include a fixed array of illumination fibers which are illuminated at different times to eject samples from a corresponding array of points on the specimen.

8. 发明申请

WO9820166A3 DNA DIAGNOSTICS BASED ON MASS SPECTROMETRY 审中-公开
标题翻译：基于大量光谱的DNA诊断
公开(公告)号：WO9820166A3
公开(公告)日：1998-10-22
申请号：PCT/US9720444
申请日：1997-11-06
申请人： SEQUENOM INC , KOESTER HUBERT , TANG KAI , FU DONG JING , SIEGERT CARSTON W , LITTLE DANIEL P , HIGGINS G SCOTT , BRAUN ANDREAS , DAMHOFFER DEMAR BRIGITTE , JURINKE CHRISTIAN , DEN BOOM DIRK VAN , XIANG GUOBING , LOUGH DAVID M
发明人： KOESTER HUBERT , TANG KAI , FU DONG-JING , SIEGERT CARSTON W , LITTLE DANIEL P , HIGGINS G SCOTT , BRAUN ANDREAS , DAMHOFFER-DEMAR BRIGITTE , JURINKE CHRISTIAN , VAN DEN BOOM DIRK , XIANG GUOBING , LOUGH DAVID M
IPC分类号： B01J19/00 , B01L3/02 , C07B61/00 , C07F9/24 , C07H21/00 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B60/14 , G01N35/00 , G01N35/10
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00387 , B01J2219/00468 , B01J2219/00497 , B01J2219/005 , B01J2219/00504 , B01J2219/00511 , B01J2219/0052 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00659 , B01J2219/00707 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07F9/2408 , C07F9/2429 , C07H21/00 , C12Q1/6837 , C12Q1/6858 , C12Q1/6865 , C12Q1/6872 , C40B40/06 , C40B40/10 , C40B60/14 , G01N35/1067 , G01N35/1074 , G01N2035/00237 , G01N2035/1037 , G01N2035/1069 , H01J49/00 , C12Q2565/507 , C12Q2565/537 , C12Q2523/10 , C12Q2565/627 , C12Q2525/197 , C12Q2531/113 , C12Q2565/518 , C12Q2535/101
摘要： Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.

9. 发明专利

AU2002300406B9 Compositions and Methods for Immobilizing Nucleic Acids to Solid Supports 未知
公开(公告)号：AU2002300406B9
公开(公告)日：2005-11-03
申请号：AU2002300406
申请日：2002-08-02
申请人： SEQUENOM INC
发明人： KOSTER HUBERT , LOUGH DAVID M
IPC分类号： C07H021/00

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