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1. 发明申请

WO2012046981A2 REAL TIME PCR DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：单核苷酸多态性的实时PCR检测
公开(公告)号：WO2012046981A2
公开(公告)日：2012-04-12
申请号：PCT/KR2011/007294
申请日：2011-10-04
申请人： SAMSUNG TECHWIN CO., LTD.
发明人： OPDYKE, Jason , HARVEY, John
IPC分类号： C12Q1/68 , C12N15/09 , G06F19/22
CPC分类号： C12Q1/686 , C12Q2521/327 , C12Q2561/113
摘要： Disclosed are methods and kits for the detection of a polymorphism during real-time PCR. Real-time PCR amplification of a target nucleic acid sequence is performed using PCR primer primers that anneal to sequences flanking a single nucleotide polymorphism (SNP) of interest. The real-time PCR reaction includes a labeled probe comprising a RNA sequence that is designed to anneal to DNA sequences at the location of the SNP. An RNA:DNA heteroduplex can then form between the SNP in the PCR fragment and the probe's RNA sequences that are complementary to the SNP. RNase H cleavage of the RNA sequence in the RNA:DNA heteroduplex results in increase in intensity of the signal generated from the label that is indicative of the presence of an SNP in the target nucleic acid.
摘要翻译：公开了用于在实时PCR期间检测多态性的方法和试剂盒。靶核酸序列的实时PCR扩增使用PCR引物引物进行，所述PCR引物引物与感兴趣的单核苷酸多态性（SNP）侧翼序列退火。实时PCR反应包括含有RNA序列的标记探针，所述RNA序列被设计为与SNP位置处的DNA序列退火。然后可以在PCR片段中的SNP和与SNP互补的探针的RNA序列之间形成RNA：DNA异源双链体。核糖核酸酶H切割RNA：DNA异源双链体中的RNA序列导致由标签产生的信号强度增加，这表明目标核酸中存在SNP。

2. 发明申请

WO2011149255A9 MODIFIED RNASE H AND DETECTION OF NUCLEIC ACID AMPLIFICATION 审中-公开
公开(公告)号：WO2011149255A9
公开(公告)日：2011-12-01
申请号：PCT/KR2011/003811
申请日：2011-05-25
申请人： SAMSUNG TECHWIN CO., LTD.
发明人： CHEUNG, Win Den , OPDYKE, Jason
IPC分类号： C12N9/22 , C12Q1/34 , C12Q1/68 , C12N15/10
摘要： A reversibly modified 'hot start' RNAse H enzyme composition is described for the improved CATACLEAVE TM probe detection of nucleic acid sequences in a test sample. A key feature of the enzyme composition is the ability to regulate the catalytic activity of the RNAse H during the course of a reverse transcription-PCR cycle. Thus, RNAse H activity can be initially suppressed to minimize degradation of RNA:DNA primer heteroduplexes prior to reverse transcription. After cDNA synthesis is complete, RNAse H activity is induced to promote the cleavage and fluorescent detection of CATACLEAVE TM probes that anneal to target DNA sequences within the reverse transcriptase-PCR products. The inducible RNAse H enzyme is amenable to high throughput applications requiring one step reverse transcriptase CATACLEAVE TM PCR in a single reaction mix.

3. 发明申请

WO2013168924A1 NUCLEIC ACID DETECTION BY OLIGONUCLEOTIDE PROBES CLEAVED BY BOTH EXONUCLEASE AND ENDONUCLEASE 审中-公开
标题翻译：由外源核酸酶和内切酶清除的寡核苷酸探针的核酸检测
公开(公告)号：WO2013168924A1
公开(公告)日：2013-11-14
申请号：PCT/KR2013/003769
申请日：2013-05-01
申请人： SAMSUNG TECHWIN CO., LTD
发明人： LI, Jun , CHEUNG, Win-Den , OPDYKE, Jason , HARVEY, John , CHONG, Song-chun
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/706 , C12Q1/6851 , C12Q1/689 , C12Q2521/301 , C12Q2521/319 , C12Q2521/327 , C12Q2565/1015
摘要： Disclosed is a method in the fields of biochemistry and molecular biology. The method is related to improve cleavage kinetics of labeled oligonucleotide probes and, consequently, increases signal-to-noise ratio in detecting nucleic acids.
摘要翻译：公开了生物化学和分子生物学领域的一种方法。该方法涉及改善标记的寡核苷酸探针的切割动力学，因此增加检测核酸中的信噪比。

4. 发明公开

EP2625290A2 REAL TIME PCR DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：个人核苷酸多态性进行实时PCR检测
公开(公告)号：EP2625290A2
公开(公告)日：2013-08-14
申请号：EP11830866.7
申请日：2011-10-04
申请人： Samsung Techwin Co.,Ltd.
发明人： OPDYKE, Jason , HARVEY, John
IPC分类号： C12Q1/68 , C12N15/09 , G06F19/22
CPC分类号： C12Q1/686 , C12Q2521/327 , C12Q2561/113
摘要： Disclosed are methods and kits for the detection of a polymorphism during real-time PCR. Real-time PCR amplification of a target nucleic acid sequence is performed using PCR primer primers that anneal to sequences flanking a single nucleotide polymorphism (SNP) of interest. The real-time PCR reaction includes a labeled probe comprising a RNA sequence that is designed to anneal to DNA sequences at the location of the SNP. An RNA:DNA heteroduplex can then form between the SNP in the PCR fragment and the probe's RNA sequences that are complementary to the SNP. RNase H cleavage of the RNA sequence in the RNA:DNA heteroduplex results in increase in intensity of the signal generated from the label that is indicative of the presence of an SNP in the target nucleic acid.

5. 发明公开

EP2576774A2 MODIFIED RNASE H AND DETECTION OF NUCLEIC ACID AMPLIFICATION 有权转让
标题翻译：修饰的RNA酶H和核酸扩增检测
公开(公告)号：EP2576774A2
公开(公告)日：2013-04-10
申请号：EP11786887.7
申请日：2011-05-25
申请人： Samsung Techwin Co., Ltd
发明人： CHEUNG, Win Den , OPDYKE, Jason
IPC分类号： C12N9/22 , C12Q1/34 , C12Q1/68 , C12N15/10
CPC分类号： C12Q1/686 , C12N9/22 , C12Q1/6848 , C12Q2521/327
摘要： A reversibly modified 'hot start' RNAse H enzyme composition is described for the improved CATACLEAVE
TM probe detection of nucleic acid sequences in a test sample. A key feature of the enzyme composition is the ability to regulate the catalytic activity of the RNAse H during the course of a reverse transcription-PCR cycle. Thus, RNAse H activity can be initially suppressed to minimize degradation of RNA:DNA primer heteroduplexes prior to reverse transcription. After cDNA synthesis is complete, RNAse H activity is induced to promote the cleavage and fluorescent detection of CATACLEAVE
TM probes that anneal to target DNA sequences within the reverse transcriptase-PCR products. The inducible RNAse H enzyme is amenable to high throughput applications requiring one step reverse transcriptase CATACLEAVE
TM PCR in a single reaction mix.
摘要翻译：描述了可逆修饰的“热启动”RNA酶H酶组合物用于改进的CATACLEAVETM探针检测测试样品中的核酸序列。酶组合物的关键特征是在逆转录-PCR循环过程中调节RNA酶H的催化活性的能力。因此，可以首先抑制RNA酶H活性，以在逆转录之前使RNA：DNA引物异源双链体的降解最小化。在cDNA合成完成后，诱导RNA酶H活性以促进与逆转录酶-PCR产物内的靶DNA序列退火的CATACLEAVE TM探针的切割和荧光检测。诱导型RNA酶H酶适用于需要在单一反应混合物中进行一步逆转录酶CATACLEAVE TM PCR的高通量应用。

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