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1. 发明申请

WO2012046981A2 REAL TIME PCR DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：单核苷酸多态性的实时PCR检测
公开(公告)号：WO2012046981A2
公开(公告)日：2012-04-12
申请号：PCT/KR2011/007294
申请日：2011-10-04
申请人： SAMSUNG TECHWIN CO., LTD.
发明人： OPDYKE, Jason , HARVEY, John
IPC分类号： C12Q1/68 , C12N15/09 , G06F19/22
CPC分类号： C12Q1/686 , C12Q2521/327 , C12Q2561/113
摘要： Disclosed are methods and kits for the detection of a polymorphism during real-time PCR. Real-time PCR amplification of a target nucleic acid sequence is performed using PCR primer primers that anneal to sequences flanking a single nucleotide polymorphism (SNP) of interest. The real-time PCR reaction includes a labeled probe comprising a RNA sequence that is designed to anneal to DNA sequences at the location of the SNP. An RNA:DNA heteroduplex can then form between the SNP in the PCR fragment and the probe's RNA sequences that are complementary to the SNP. RNase H cleavage of the RNA sequence in the RNA:DNA heteroduplex results in increase in intensity of the signal generated from the label that is indicative of the presence of an SNP in the target nucleic acid.
摘要翻译：公开了用于在实时PCR期间检测多态性的方法和试剂盒。靶核酸序列的实时PCR扩增使用PCR引物引物进行，所述PCR引物引物与感兴趣的单核苷酸多态性（SNP）侧翼序列退火。实时PCR反应包括含有RNA序列的标记探针，所述RNA序列被设计为与SNP位置处的DNA序列退火。然后可以在PCR片段中的SNP和与SNP互补的探针的RNA序列之间形成RNA：DNA异源双链体。核糖核酸酶H切割RNA：DNA异源双链体中的RNA序列导致由标签产生的信号强度增加，这表明目标核酸中存在SNP。

2. 发明申请

WO2013168924A1 NUCLEIC ACID DETECTION BY OLIGONUCLEOTIDE PROBES CLEAVED BY BOTH EXONUCLEASE AND ENDONUCLEASE 审中-公开
标题翻译：由外源核酸酶和内切酶清除的寡核苷酸探针的核酸检测
公开(公告)号：WO2013168924A1
公开(公告)日：2013-11-14
申请号：PCT/KR2013/003769
申请日：2013-05-01
申请人： SAMSUNG TECHWIN CO., LTD
发明人： LI, Jun , CHEUNG, Win-Den , OPDYKE, Jason , HARVEY, John , CHONG, Song-chun
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/706 , C12Q1/6851 , C12Q1/689 , C12Q2521/301 , C12Q2521/319 , C12Q2521/327 , C12Q2565/1015
摘要： Disclosed is a method in the fields of biochemistry and molecular biology. The method is related to improve cleavage kinetics of labeled oligonucleotide probes and, consequently, increases signal-to-noise ratio in detecting nucleic acids.
摘要翻译：公开了生物化学和分子生物学领域的一种方法。该方法涉及改善标记的寡核苷酸探针的切割动力学，因此增加检测核酸中的信噪比。

3. 发明公开

EP2625290A2 REAL TIME PCR DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：个人核苷酸多态性进行实时PCR检测
公开(公告)号：EP2625290A2
公开(公告)日：2013-08-14
申请号：EP11830866.7
申请日：2011-10-04
申请人： Samsung Techwin Co.,Ltd.
发明人： OPDYKE, Jason , HARVEY, John
IPC分类号： C12Q1/68 , C12N15/09 , G06F19/22
CPC分类号： C12Q1/686 , C12Q2521/327 , C12Q2561/113
摘要： Disclosed are methods and kits for the detection of a polymorphism during real-time PCR. Real-time PCR amplification of a target nucleic acid sequence is performed using PCR primer primers that anneal to sequences flanking a single nucleotide polymorphism (SNP) of interest. The real-time PCR reaction includes a labeled probe comprising a RNA sequence that is designed to anneal to DNA sequences at the location of the SNP. An RNA:DNA heteroduplex can then form between the SNP in the PCR fragment and the probe's RNA sequences that are complementary to the SNP. RNase H cleavage of the RNA sequence in the RNA:DNA heteroduplex results in increase in intensity of the signal generated from the label that is indicative of the presence of an SNP in the target nucleic acid.

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