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1. 发明授权

US07316770B2 Method of electrophoresis, electrophoresis apparatus and capillary array 有权
标题翻译：电泳方法，电泳装置和毛细管阵列
公开(公告)号：US07316770B2
公开(公告)日：2008-01-08
申请号：US09814860
申请日：2001-03-23
申请人： Ryoji Inaba , Tomoyuki Sakai , Satoshi Takahashi , Muneo Maeshima , Miho Ozawa , Masaya Kojima , Daizo Tokinaga
发明人： Ryoji Inaba , Tomoyuki Sakai , Satoshi Takahashi , Muneo Maeshima , Miho Ozawa , Masaya Kojima , Daizo Tokinaga
IPC分类号： G01N27/26
CPC分类号： G01N27/44708 , G01N27/44782
摘要： A method of electrophoresis, an electrophoresis apparatus and a capillary array in which a fluorescently labelled sample is supplied into capillaries in a capillary array constituted by a plurality of capillaries and including a sample supply portion, an electrophoresis medium supply portion and a detection portion while controlling the temperature of the capillary array through gas circulation, the sample is caused to migrate and separated in the capillaries through electrophoresis, and at the region where the capillaries are contacted or come close each other a plurality of capillaries are contacted to a solid body so as to dissipate heat generated from the capillaries to the solid body.
摘要翻译：一种电泳方法，电泳装置和毛细管阵列，其中荧光标记的样品被供应到由多个毛细管构成的毛细管阵列的毛细管中，并且包括样品供应部分，电泳介质供应部分和检测部分，同时控制通过气体循环的毛细管阵列的温度，通过电泳使样品在毛细管中迁移和分离，并且在毛细管接触或彼此靠近的区域，多个毛细管与固体接触，以便以将从毛细管产生的热量散发到固体。

2. 发明申请

US20050067285A1 Electrophoresis device 审中-公开
标题翻译：电泳装置
公开(公告)号：US20050067285A1
公开(公告)日：2005-03-31
申请号：US10913517
申请日：2004-08-09
申请人： Ryoji Inaba , Miho Ozawa , Akihiro Suzuki , Daizo Tokinaga , Tomohiro Shoji , Yoshitaka Kodama , Isao Haraura , Masaya Kojima
发明人： Ryoji Inaba , Miho Ozawa , Akihiro Suzuki , Daizo Tokinaga , Tomohiro Shoji , Yoshitaka Kodama , Isao Haraura , Masaya Kojima
IPC分类号： G01N21/64 , G01N27/447 , G01N27/453
CPC分类号： G01N27/44782 , G01N27/44721
摘要： The present invention is related to the decrease of crosstalk, in which part of light emission from a specific capillary is overlayed on the light emission position of its adjoining capillaries and is detected as signal from adjacent capillaries. Study conducted by the inventor has found that the crosstalk contains at least the following components. The signal light from a capillary may propagate through an adjoining capillary or a plurality of capillaries, the reflection at the inner surface of the outer diameter of quartz capillary makes plural signal paths to the photodetector. The crosstalk component may be focused at a point at a predetermined distance from the center axis of the capillary. The present invention is aimed at the control of the range of image to be detected as the capillary signal among the images focused on the photodetector from the capillaries. The present invention may decrease the crosstalk by controlling the detection of the light emission from the adjacent capillaries.
摘要翻译：本发明涉及串扰的减少，其中来自特定毛细管的光发射的一部分覆盖在其相邻的毛细管的发光位置上，并被检测为来自相邻毛细管的信号。发明人进行的研究发现串扰至少包含以下组件。来自毛细管的信号光可以通过邻接的毛细管或多个毛细管传播，石英毛细管的外径的内表面的反射使得到达光电检测器的多个信号路径。串扰分量可以聚焦在距离毛细管的中心轴预定距离的点处。本发明的目的在于控制从毛细管聚焦在光电检测器上的图像中的毛细管信号的被检测图像的范围。本发明可以通过控制来自相邻毛细管的光发射的检测来减小串扰。

3. 发明申请

US20060076239A1 Electrophoresis apparatus 审中-公开
标题翻译：电泳仪
公开(公告)号：US20060076239A1
公开(公告)日：2006-04-13
申请号：US11245491
申请日：2005-10-07
申请人： Ryoji Inaba , Motohiro Yamazaki , Akihiro Suzuki , Miho Ozawa , Daizo Tokinaga , Tomonari Morioka , Seiichi Ugai
发明人： Ryoji Inaba , Motohiro Yamazaki , Akihiro Suzuki , Miho Ozawa , Daizo Tokinaga , Tomonari Morioka , Seiichi Ugai
IPC分类号： G01N27/447
CPC分类号： G01N27/447
摘要： In an electrophoresis apparatus comprising capillaries for containing therein fluorescence-labeled substances, and a sensor for irradiating the capillaries with exciting light and detecting fluorescences, the sensor has a substrate including a datum surface for holding thereon the capillaries, and a groove sinking from the datum surface, and the groove has a plurality of reflecting surfaces for reflecting thereon the fluorescences emitted from the capillaries by a plurality of times.
摘要翻译：在包括用于在其中含有荧光标记物质的毛细管的电泳装置和用于用激发光照射毛细管并检测荧光的传感器中，传感器具有包括用于保持毛细管的基准表面的基底和从基准面凹下的凹槽表面，并且凹槽具有多个反射表面，用于在其上反射从毛细管发射的荧光多次。

4. 发明授权

US08142635B2 Capillary array 有权
标题翻译：毛细管阵列
公开(公告)号：US08142635B2
公开(公告)日：2012-03-27
申请号：US12843103
申请日：2010-07-26
申请人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
发明人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
IPC分类号： C02F1/40
CPC分类号： G01N27/44721 , G01N27/44782
摘要： A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.
摘要翻译：毛细管阵列包括作为电泳所需功能的光检测部分，样品供应部分，缓冲溶液供应部分和电压施加部分，从而当将毛细管阵列组装到电泳装置中时可以立即使用。因此，提供了可以容易地并入电泳装置中的毛细管阵列。

5. 发明授权

US07785458B2 Capillary array 有权
标题翻译：毛细管阵列
公开(公告)号：US07785458B2
公开(公告)日：2010-08-31
申请号：US11300377
申请日：2005-12-15
申请人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
发明人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
IPC分类号： C02F1/40
CPC分类号： G01N27/44721 , G01N27/44782
摘要： A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.
摘要翻译：毛细管阵列包括作为电泳所需功能的光检测部分，样品供应部分，缓冲溶液供应部分和电压施加部分，从而当将毛细管阵列组装到电泳装置中时可以立即使用。因此，提供了可以容易地并入电泳装置中的毛细管阵列。

6. 发明申请

US20060091012A1 Capillary array 有权
标题翻译：毛细管阵列
公开(公告)号：US20060091012A1
公开(公告)日：2006-05-04
申请号：US11300377
申请日：2005-12-15
申请人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
发明人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
IPC分类号： C02F1/40
CPC分类号： G01N27/44721 , G01N27/44782
摘要： A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.
摘要翻译：毛细管阵列包括作为电泳所需功能的光检测部分，样品供应部分，缓冲溶液供应部分和电压施加部分，从而当将毛细管阵列组装到电泳装置中时可以立即使用。因此，提供了可以容易地并入电泳装置中的毛细管阵列。

7. 发明授权

US07005052B2 Capillary array 有权
公开(公告)号：US07005052B2
公开(公告)日：2006-02-28
申请号：US09845303
申请日：2001-05-01
申请人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
发明人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
IPC分类号： G01N27/447 , C02F1/40
CPC分类号： G01N27/44721 , G01N27/44782
摘要： A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.

8. 发明申请

US20100288642A1 CAPILLARY ARRAY 有权
标题翻译：毛细管阵列
公开(公告)号：US20100288642A1
公开(公告)日：2010-11-18
申请号：US12843103
申请日：2010-07-26
申请人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
发明人： Yasushi Shimizu , Toshiaki Kita , Kiyoshi Tsukada , Akihiro Suzuki , Tomonari Morioka , Masaya Kojima , Ryoji Inaba , Daizo Tokinaga , Shuhei Yamamoto , Yoshiyuki Okishima , Masao Fukunaga , Takayasu Furukawa , Tomohiro Shoji
IPC分类号： G01N27/26
CPC分类号： G01N27/44721 , G01N27/44782
摘要： A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.
摘要翻译：毛细管阵列包括作为电泳所需功能的光检测部分，样品供应部分，缓冲溶液供应部分和电压施加部分，从而当将毛细管阵列组装到电泳装置中时可以立即使用。因此，提供了可以容易地并入电泳装置中的毛细管阵列。

9. 发明授权

US5308990A Method for measuring microparticles, quantitative measuring method therefor and instrument for measuring microparticles 失效
标题翻译：测量微粒的方法，其定量测量方法和测量微粒的仪器
公开(公告)号：US5308990A
公开(公告)日：1994-05-03
申请号：US882954
申请日：1992-05-14
申请人： Satoshi Takahashi , Daizo Tokinaga , Kazunori Okano
发明人： Satoshi Takahashi , Daizo Tokinaga , Kazunori Okano
IPC分类号： F02B75/02 , G01N15/14
CPC分类号： G01N15/1459 , F02B2075/027 , G01N2015/0092 , G01N2015/1486 , G01N2021/6439
摘要： A microparticle measuring method according to the present invention, by which the number of fluorescent microparticles is counted and the fluorescent microparticles are analyzed, includes the steps of introducing fluorescent microparticles into a narrow flow path almost one after another; irradiating the fluorescent microparticles in the narrow flow path with excitation light; detecting a signal pulse produced by detection of a single photon of fluorescence generated by the irradiation with the excitation light; and recognizing existence of the fluorescent microparticle, starting from the number of signal pulses measured per predetermined standard period, and further includes the step of obtaining the number of signal pulses per standard period with a time interval shorter than the standard period. It further includes the step of counting successively the number of signal pulses generated in the predetermined standard period to recognize existence of the fluorescent microparticle, when the count value exceeds a predetermined threshold, and the kind of the fluorescent microparticles is estimated from the count value. Particularly by using microparticles having a diameter smaller than 0.1 .mu.m as label material, reaction efficiency of the label material is increased, stability of the binding with the material to be measured is raised, and the material to be measured can be detected with a high precision and a high sensitivity.
摘要翻译：根据本发明的微粒测量方法，其中计数荧光微粒的数量并分析荧光微粒，包括几乎一个接一个地将荧光微粒引入窄流路的步骤; 用激发光照射窄流路中的荧光微粒; 检测通过用激发光照射产生的荧光的单个光子的检测而产生的信号脉冲; 并且从每个预定标准周期测量的信号脉冲数开始识别荧光微粒的存在，并且还包括以比标准周期短的时间间隔获得每标准周期的信号脉冲数的步骤。还包括当计数值超过预定阈值时，连续计数在预定标准周期中产生的信号脉冲数，以识别荧光微粒的存在的步骤，并根据计数值估计荧光微粒的种类。特别是通过使用直径小于0.1μm的微粒作为标签材料，标签材料的反应效率提高，与被测定材料的结合的稳定性提高，可以高检出被测量的材料精度高，灵敏度高。

10. 发明授权

US5057438A Electrophoretic antigen-antibody determination with laminate of multiple membranes 失效
标题翻译：电泳抗原 - 抗体用多层膜叠层测定
公开(公告)号：US5057438A
公开(公告)日：1991-10-15
申请号：US31665
申请日：1987-03-30
申请人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
发明人： Kazumichi Imai , Daizo Tokinaga , Teruaki Kobayashi , Kenji Yasuda , Keiichi Nagai , Satoshi Takahashi
IPC分类号： G01N33/561
CPC分类号： G01N33/561 , Y10S436/809 , Y10S436/824
摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.
摘要翻译：通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。

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