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1. 发明授权

US5119588A Method and apparatus for culturing autotrophic plants from heterotrophic plant material 失效
标题翻译：用于培育来自异种植物材料的自植物的方法和装置
公开(公告)号：US5119588A
公开(公告)日：1992-06-09
申请号：US589052
申请日：1990-09-27
申请人： Roger Timmis , Mary E. Kreitinger , Michael J. Yancey
发明人： Roger Timmis , Mary E. Kreitinger , Michael J. Yancey
IPC分类号： A01H4/00 , C12N5/04
CPC分类号： C12N5/04 , A01H4/001
摘要： A method and apparatus is disclosed for growing embryonic or other undeveloped plant material in a controlled, biologically sterile environment to produce plants capable of surviving in soil in an uncontrolled environment. Cloned somatic embryos of a particular plant are individually embeddeed in separate sterile plugs comprised of a soil-like particulate medium. A volume of aqueous medium comprising a source of carbon and energy for the somatic embryos, and plant growth regulating substances, if required, is added to each plug. The sterile plugs are then isolated from the ambient environment while permitting exposure to light and atmospheric gases sufficient to effect development of photosynthesis in the developing embryos in the plugs. While in such isolation, the resulting plantlets develop roots and shoots. The resulting autotrophic plants are then removed from isolation and the growth medium is altered to remove the carbon and energy source to limit the capacity of the medium to support plant harming microbes. The plants are then transplanted. Several embodiments are disclosed involving compartmentalized trays and covering films or bags by which embryo-containing plugs are kept isolated from each other and from the environment during development of the embryos.
摘要翻译：公开了用于在受控的生物无菌环境中生长胚胎或其他未开发的植物材料以产生能够在不受控制的环境中在土壤中存活的植物的方法和装置。将特定植物的克隆的体细胞胚分别嵌入由包含土壤样颗粒介质的单独的无菌塞子中。如果需要，将一定体积的含有体细胞胚的碳源和能量的水性介质和植物生长调节物质加入到每个塞子中。然后将无菌塞从环境环境中分离，同时允许暴露于足以在插塞中发育的胚胎中发展光合作用的光和大气。在这种隔离下，所产生的小植株发育出根和芽。然后从分离中除去所得的自养植物，改变生长培养基以除去碳和能源，以限制培养基支持植物伤害微生物的能力。然后移植植物。公开了几个实施例，其涉及分隔的托盘和覆盖膜或袋，通过该薄膜或袋，含有胚胎的塞子在胚胎发育期间彼此隔离并且与环境保持隔离。

2. 发明授权

US07685767B2 Automated system and method for harvesting and multi-stage screening of plant embryos 有权
标题翻译：用于植物胚胎收获和多期筛选的自动化系统和方法
公开(公告)号：US07685767B2
公开(公告)日：2010-03-30
申请号：US11778766
申请日：2007-07-17
申请人： Roger Timmis , Edwin Hirahara , Harry G Folster , Heather Surerus-Lopez
发明人： Roger Timmis , Edwin Hirahara , Harry G Folster , Heather Surerus-Lopez
IPC分类号： A01C1/06 , A01C21/00
CPC分类号： A01H4/00 , A01H1/04 , A01H4/006 , C12N5/04 , G01N15/147 , G01N15/1475 , G01N2015/0019 , G01N2015/1497 , Y10S47/09
摘要： A method and system for automatically harvesting and screening plant embryos in multiple stages to identify those embryos that are suited for incorporation into manufactured seeds are provided. The method includes generally three steps. First, plant embryos are automatically sorted according to their rough size/shape and also singulated into discrete embryo units, for example by vibrational sieving. Second, the sorted and singulated plant embryos are classified using a first classification method. For example, each embryo may be imaged by a camera and the image is used to ascertain the embryo's more precise size/shape. Third, for those embryos that have passed the first classification method, a second classification method is applied. For example, a pre-developed classification algorithm to classify embryos according to their putative germination vigor may be applied to the same image used in the first classification method, to identify those embryos that are likely to germinate.
摘要翻译：提供了一种用于自动收获和筛选多个阶段的植物胚胎的方法和系统，以鉴定适合于掺入生产的种子的那些胚胎。该方法通常包括三个步骤。首先，植物胚胎根据其粗糙尺寸/形状自动分类，并且通过振动筛选也分离成离散的胚胎单位。第二，使用第一种分类方法分类和分割的植物胚胎。例如，可以通过照相机对每个胚胎进行成像，并且使用该图像来确定胚胎更精确的尺寸/形状。第三，对于已经通过第一种分类方法的那些胚胎，应用第二种分类方法。例如，可以将根据其推定的发芽活力对胚胎进行分类的预先开发的分类算法应用于在第一种分类方法中使用的相同图像，以鉴定可能发芽的胚胎。

3. 发明授权

US07530197B2 Automated system and method for harvesting and multi-stage screening of plant embryos 有权
标题翻译：用于植物胚胎收获和多期筛选的自动化系统和方法
公开(公告)号：US07530197B2
公开(公告)日：2009-05-12
申请号：US10853491
申请日：2004-05-24
申请人： Roger Timmis , Edwin Hirahara , Harry G. Folster , Heather Surerus-Lopez
发明人： Roger Timmis , Edwin Hirahara , Harry G. Folster , Heather Surerus-Lopez
IPC分类号： A01C1/06 , A01C21/00 , A01C1/00 , A01B79/00 , A01B79/02 , A01G1/00 , A01H3/00
CPC分类号： A01H4/00 , A01H1/04 , A01H4/006 , C12N5/04 , G01N15/147 , G01N15/1475 , G01N2015/0019 , G01N2015/1497 , Y10S47/09
摘要： A method and system for automatically harvesting and screening plant embryos in multiple stages to identify those embryos that are suited for incorporation into manufactured seeds are provided. The method includes generally three steps. First, plant embryos are automatically sorted according to their rough size/shape and also singulated into discrete embryo units, for example by vibrational sieving. Second, the sorted and singulated plant embryos are classified using a first classification method. For example, each embryo may be imaged by a camera and the image is used to ascertain the embryo's more precise size/shape. Third, for those embryos that have passed the first classification method, a second classification method is applied. For example, a pre-developed classification algorithm to classify embryos according to their putative germination vigor may be applied to the same image used in the first classification method, to identify those embryos that are likely to germinate.
摘要翻译：提供了一种用于自动收获和筛选多个阶段的植物胚胎的方法和系统，以鉴定适合于掺入生产的种子的那些胚胎。该方法通常包括三个步骤。首先，植物胚胎根据其粗糙尺寸/形状自动分类，并且通过振动筛选也分离成离散的胚胎单位。第二，使用第一种分类方法分类和分割的植物胚胎。例如，可以通过照相机对每个胚胎进行成像，并且使用图像来确定胚胎更精确的大小/形状。第三，对于已经通过第一种分类方法的那些胚胎，应用第二种分类方法。例如，可以将根据其推定的发芽活力对胚胎进行分类的预先开发的分类算法应用于在第一种分类方法中使用的相同图像，以鉴定可能发芽的胚胎。

4. 发明申请

US20060143731A1 Methods for processing image and/or spectral data for enhanced embryo classification 有权
标题翻译：用于加强胚胎分类的图像和/或光谱数据的处理方法
公开(公告)号：US20060143731A1
公开(公告)日：2006-06-29
申请号：US11297919
申请日：2005-12-08
申请人： Roger Timmis , Mitchell Toland
发明人： Roger Timmis , Mitchell Toland
IPC分类号： G06K9/00 , A01H1/00
CPC分类号： G06K9/0014 , G06K9/00147
摘要： A method is disclosed for classifying plant embryos according to their quality using a logistic regression model. First, sets of image or spectral data are acquired from plant embryos of known quality, respectively. Second, each of the acquired sets of image or spectral data is associated with one of multiple class labels according to the corresponding embryo's known quality. Third the sets of image or spectral data values are filtered to provide filtered image or spectral data values. Fourth, a classification algorithm, e.g., a logistic regression analysis is applied to the filtered data values and their corresponding class labels to develop a classification model. Fifth, image or spectral data are acquired from a plant embryo of unknown quality, and filtered data values are derived therefrom. Sixth, the classification model is applied to the filtered data values for the plant embryo of unknown quality to classify the same
摘要翻译：公开了一种使用逻辑回归模型根据其质量对植物胚胎进行分类的方法。首先，分别从已知质量的植物胚胎获取图像或光谱数据集。其次，根据相应的胚胎的已知质量，所获取的图像或光谱数据集合与多个类标签中的一个相关联。第三，对图像或光谱数据值集合进行滤波以提供滤波图像或光谱数据值。第四，将分类算法，例如逻辑回归分析应用于过滤的数据值及其对应的类标签，以开发分类模型。第五，从未知质量的植物胚胎获取图像或光谱数据，并从其中导出滤波数据值。第六，将分类模型应用于未知质量的植物胚胎的过滤数据值进行分类

5. 发明授权

US08744775B2 Methods for classification of somatic embryos comprising hyperspectral line imaging 有权
标题翻译：包括高光谱线成像的体细胞胚分类方法
公开(公告)号：US08744775B2
公开(公告)日：2014-06-03
申请号：US12317560
申请日：2008-12-23
申请人： Roger Timmis , Paul R. Spencer
发明人： Roger Timmis , Paul R. Spencer
IPC分类号： G01N33/48
CPC分类号： G01J3/2823 , G01J3/02 , G01J3/0216 , G01J3/0218 , G01J3/10 , G01J3/14 , G01J3/18 , G01J3/28 , G01N21/3563 , G01N21/359 , G02B21/365
摘要： The present invention relates to methods, apparatus, and imaging systems for using near-infrared spectroscopy imaging of plant embryos for classifying plant embryos. In one embodiment, a method is provided for classifying a plant embryo of an unknown type based on near infrared spectroscopy imaging.
摘要翻译：本发明涉及使用植物胚胎的近红外光谱成像来分类植物胚胎的方法，装置和成像系统。在一个实施方案中，提供了一种基于近红外光谱成像来分类未知类型的植物胚胎的方法。

6. 发明授权

US07610155B2 Methods for processing spectral data for enhanced embryo classification 有权
标题翻译：用于处理增强胚胎分类的光谱数据的方法
公开(公告)号：US07610155B2
公开(公告)日：2009-10-27
申请号：US11297919
申请日：2005-12-08
申请人： Roger Timmis , Mitchell R. Toland
发明人： Roger Timmis , Mitchell R. Toland
IPC分类号： G01N33/48 , G01N33/50
CPC分类号： G06K9/0014 , G06K9/00147
摘要： A method is disclosed for classifying plant embryos according to their quality using a logistic regression model. First, sets of image or spectral data are acquired from plant embryos of known quality, respectively. Second, each of the acquired sets of image or spectral data is associated with one of multiple class labels according to the corresponding embryo's known quality. Third the sets of image or spectral data values are filtered to provide filtered image or spectral data values. Fourth, a classification algorithm, e.g., a logistic regression analysis is applied to the filtered data values and their corresponding class labels to develop a classification model. Fifth, image or spectral data are acquired from a plant embryo of unknown quality, and filtered data values are derived therefrom. Sixth, the classification model is applied to the filtered data values for the plant embryo of unknown quality to classify the same.
摘要翻译：公开了一种使用逻辑回归模型根据其质量对植物胚胎进行分类的方法。首先，分别从已知质量的植物胚胎获取图像或光谱数据集。其次，根据相应的胚胎的已知质量，获得的图像或光谱数据集合中的每一个与多个类别标签之一相关联。第三，对图像或光谱数据值集合进行滤波以提供滤波图像或光谱数据值。第四，将分类算法，例如逻辑回归分析应用于过滤的数据值及其对应的类标签，以开发分类模型。第五，从未知质量的植物胚胎获取图像或光谱数据，并从其中导出滤波数据值。第六，将分类模型应用于未知质量的植物胚胎的过滤数据值进行分类。

7. 发明申请

US20090171591A1 Methods for classification of somatic embryos comprising hyperspectral line imaging 有权
标题翻译：包括高光谱线成像的体细胞胚分类方法
公开(公告)号：US20090171591A1
公开(公告)日：2009-07-02
申请号：US12317560
申请日：2008-12-23
申请人： Roger Timmis , Paul R. Spencer
发明人： Roger Timmis , Paul R. Spencer
IPC分类号： G01N33/48 , G01J3/12 , G02B5/02
CPC分类号： G01J3/2823 , G01J3/02 , G01J3/0216 , G01J3/0218 , G01J3/10 , G01J3/14 , G01J3/18 , G01J3/28 , G01N21/3563 , G01N21/359 , G02B21/365
摘要： The present invention relates to methods, apparatus, and imaging systems for using near-infrared spectroscopy imaging of plant embryos for classifying plant embryos. In one embodiment, a method is provided for classifying a plant embryo of an unknown type based on near infrared spectroscopy imaging.
摘要翻译：本发明涉及使用植物胚胎的近红外光谱成像来分类植物胚胎的方法，装置和成像系统。在一个实施方案中，提供了一种基于近红外光谱成像来分类未知类型的植物胚胎的方法。

8. 发明申请

US20060160065A1 Method for classifying plant embryos using Raman spectroscopy 审中-公开
标题翻译：使用拉曼光谱分类植物胚胎的方法
公开(公告)号：US20060160065A1
公开(公告)日：2006-07-20
申请号：US11323404
申请日：2005-12-29
申请人： Roger Timmis , Mitchell Toland , Timnit Ghermay , Brian Penttila , Carolyn Carpenter
发明人： Roger Timmis , Mitchell Toland , Timnit Ghermay , Brian Penttila , Carolyn Carpenter
IPC分类号： C12Q1/00
CPC分类号： A01C1/00 , A01H1/04 , A01H4/00 , G06K9/00147 , G06K9/00557 , G06K9/6215
摘要： A three-step method for classifying plant embryo quality using Raman spectroscopy is provided. First, a classification model is developed based on Raman spectral data of reference samples of plant embryos or any portions of plant embryos of known embryo quality. The embryo quality may be known based on a comparison to a normal zygotic embryo or on actual planting of the embryo to observe its germination and subsequent growth. Then, a data analysis is carried out by applying one or more classification algorithms to the acquired Raman spectral data to develop a classification model. Second, Raman spectral data of a plant embryo or any portion of a plant embryo of unknown embryo quality are obtained. Third, the classification model developed in the first step is applied to the Raman spectral data obtained from the embryo (or any portions thereof) of unknown quality to classify the quality of this plant embryo.
摘要翻译：提供了使用拉曼光谱分类植物胚胎质量的三步法。首先，基于植物胚胎的参考样品或已知胚胎质量的植物胚胎的任何部分的拉曼光谱数据开发分类模型。胚胎质量可以基于与正常合子胚胎的比较或胚胎的实际种植来观察其发芽和随后的生长而已知。然后，通过对获取的拉曼光谱数据应用一个或多个分类算法来开发分类模型来进行数据分析。第二，获得植物胚胎或未知胚胎质量的植物胚胎的任何部分的拉曼光谱数据。第三，将第一步开发的分类模型应用于从未知质量的胚胎（或其任何部分）获得的拉曼光谱数据，以对该植物胚胎的质量进行分类。

9. 发明申请

US20090007303A1 Methods for increasing germination vigor by early singulation of conifer somatic embryos 有权
标题翻译：通过早期分选针叶树体细胞胚增加萌发活力的方法
公开(公告)号：US20090007303A1
公开(公告)日：2009-01-01
申请号：US12164291
申请日：2008-06-30
申请人： Roger Timmis , James A. Grob , Pramod K. Gupta , Susan D. Rayfield
发明人： Roger Timmis , James A. Grob , Pramod K. Gupta , Susan D. Rayfield
IPC分类号： A01H5/00
CPC分类号： A01H1/04 , A01C1/02 , A01H3/00 , A01H3/04 , A01H4/001 , A01H4/008
摘要： In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.
摘要翻译：在一个方面，提供了一种用于增加在体外产生的针叶树体细胞胚的发芽势的方法。该方法包括：（a）在第一显影培养基中的第一次孵育期后，从第一胚胎培养物中分离多个单个未成熟针叶体细胞胚; 和（b）使多个单切的未成熟针叶树体细胞胚与第二培养基接触第二潜伏期。

10. 发明申请

US20080052056A1 METHODS FOR CLASSIFICATION OF SOMATIC EMBRYOS 审中-公开
标题翻译： SOMATIC EMBRYOS分类方法
公开(公告)号：US20080052056A1
公开(公告)日：2008-02-28
申请号：US11861213
申请日：2007-09-25
申请人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
发明人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
IPC分类号： G06G7/48
CPC分类号： A01H1/04 , A01C1/00 , A01C1/025 , A01H4/00 , G01N15/1475 , G06K9/00 , G06K9/00147
摘要： The present invention is directed towards methods for the classification of plant embryos by the application of one or more classification algorithms to analyze digitized images and absorption, transmittance, or reflectance spectra. The methods are generally applicable and emphasize the importance of acquiring and using as much image and absorption, transmittance, or reflectance spectral information as possible, based on objective criteria. The present invention allows automated selection of embryos most suitable for further culture and rejection of those seen as less suitable.
摘要翻译：本发明涉及通过应用一种或多种分类算法来分析数字化图像和吸收，透射率或反射光谱来分类植物胚胎的方法。这些方法通常是适用的，并强调基于客观标准获取和使用尽可能多的图像和吸收，透射率或反射光谱信息的重要性。本发明允许自动选择最适合进一步培养和排除那些被认为不太适合的胚胎。

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