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1. 发明申请

US20030077691A1 Astaxanthin synthase 失效
公开(公告)号：US20030077691A1
公开(公告)日：2003-04-24
申请号：US10066007
申请日：2002-02-01
申请人： ROCHE VITAMINS INC.
发明人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
IPC分类号： C12P023/00 , C07H021/04 , C12N009/02 , C12N001/21 , C12P021/02
CPC分类号： C12P23/00 , C12N9/00
摘要： The present invention is directed to genetic materials useful for the preparation of astaxanthin from beta-carotene, such as polypeptides having astaxanthin synthase activity, DNA fragments coding for astaxanthin synthase, recombinant organisms and the like. Those novel genetic materials may be derived from Phaffia rhodozyma. The present invention also provides a process for the production of astaxanthin.

2. 发明申请

US20040063166A1 Production of biotin 审中-公开
标题翻译：生物素生产
公开(公告)号：US20040063166A1
公开(公告)日：2004-04-01
申请号：US10440067
申请日：2003-05-15
申请人： ROCHE VITAMINS, INC.
发明人： Tatsuo Hoshino , Akira Asakura , Tatsuya Kiyasu , Yoshie Nagahashi
IPC分类号： C12P017/18
CPC分类号： C12N9/93 , C12N9/13 , C12P17/186
摘要： The present invention provides processes for making biotin from desthiobiotin by either contacting desthiobiotin with an enzyme reaction mixture containing bioB gene product and nifU gene product and/or nifS gene product and isolating the biotin or cultivating a microorganism transformed with DNA encoding the bioB gene product, nifU gene product and nifS gene product and isolating the biotin.
摘要翻译：本发明提供通过将脱硫生物素与含有bioB基因产物和nifU基因产物和/或nifS基因产物的酶反应混合物接触来生产脱氧生物素的生物素的方法，并分离生物素或培养用编码bioB基因产物的DNA转化的微生物， nifU基因产物和nifS基因产物，并分离生物素。

3. 发明申请

US20010026933A1 Aldehyde dehydrogenase 有权
标题翻译：醛脱氢酶
公开(公告)号：US20010026933A1
公开(公告)日：2001-10-04
申请号：US09815695
申请日：2001-03-23
申请人： ROCHE VITAMINS INC.
发明人： Tatsuo Hoshino , Taro Miyazaki , Teruhide Sugisawa
IPC分类号： C12N009/04
CPC分类号： C12N9/0008 , C12P7/60
摘要： A new aldehyde dehydrogenase having the physico-chemical properties:molecular weight: 150,000null6,000 or 230,000null9,000; substrate specificity:active on aldehyde compounds; cofactors:pyrroloquinoline quinone and heme c; optimum pH: 7.0-8.5; and inhibitors: Co2null, Cu2null, Fe2null, Ni2null, Zn2null, monoiodoacetate and EDTA, is derived from a microorganism belonging to the genus Gluconobacter. Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism. 2-Keto-L-gulonic acid (2-KGA) can be produced from L-sorbosone by contacting L-sorbosone with (i) the aldehyde dehydrogenase in the presence of an electron acceptor, (ii) a Gluconobacter microorganism capable of producing the aldehyde dehydrogenase in an aqueous medium under aerobic conditions or (iii) a cell-free extract of said microorganism, and in each case isolating the resulting 2-KGA from the reaction mixture.
摘要翻译：具有物理化学性质的新型醛脱氢酶：分子量：15万6,000或23万9,000; 底物特异性：对醛化合物有活性; 辅因子：吡咯并喹啉醌和血红素c; 最适pH：7.0-8.5; 和抑制剂：Co2 +，Cu2 +，Fe2 +，Ni2 +，Zn2 +，一碘乙酸和EDTA，衍生自属于葡萄糖酸杆菌属的微生物。所述醛脱氢酶可以通过在需氧条件下在水性营养培养基中培养能够产生具有上述性质的醛脱氢酶的葡糖杆菌属的微生物，破坏微生物的细胞并从其中分离和纯化醛脱氢酶微生物破碎的细胞的无细胞提取物。 2-酮-L-古洛糖酸（2-KGA）可以由L-山梨糖酮通过在电子受体存在下将L-山梨醇与（i）醛脱氢酶接触，（ii）能产生醛脱氢酶在有氧条件下在水性介质中或（iii）所述微生物的无细胞提取物，并且在每种情况下从反应混合物中分离得到的2-KGA

4. 发明申请

US20040137584A1 Biotin biosynthetic genes 失效
标题翻译：生物素生物合成基因
公开(公告)号：US20040137584A1
公开(公告)日：2004-07-15
申请号：US10763933
申请日：2004-01-23
申请人： ROCHE VITAMINS INC.
发明人： Yasuhiro Furuichi , Tatsuo Hoshino , Hitoshi Kimura , Tatsuya Kiyasu , Yoshie Nagahashi
IPC分类号： C12P017/18 , C07H021/04 , C12N009/10
CPC分类号： C12N15/52 , A23L33/15 , A23V2002/00 , C12P17/185 , A23V2250/70
摘要： The present invention relates to the production process of biotin by fermentation using a genetically engineered microorganism, and DNA sequences and vectors to be used in such process.
摘要翻译：本发明涉及使用遗传工程微生物的发酵生物素的生产方法，以及用于该方法的DNA序列和载体。

5. 发明申请

US20030105160A1 Microbial process for producing L-ascorbic acid, D-erythorbic acid, and salts thereof 有权
标题翻译：生产L-抗坏血酸，D-异抗坏血酸及其盐的微生物方法
公开(公告)号：US20030105160A1
公开(公告)日：2003-06-05
申请号：US09938035
申请日：2001-08-23
申请人： ROCHE VITAMINS INC.
发明人： Akira Asakura , Tatsuo Hoshino , Masako Shinjoh
IPC分类号： A61K031/34 , A01N043/08 , C12P007/40
CPC分类号： C12P17/04
摘要： A process for producing L-ascorbic acid or D-erythorbic acid, or in each case its sodium, potassium or calcium salt, from 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, or in each case its sodium, potassium or calcium salt, that involves incubating 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, each as the free acid or as its sodium, potassium or calcium salt, and the cells of a thermoacidophilic microorganism at temperatures from about 30null C. to about 100null C., and at a pH from about 1 to about 6, in a solution, to form L-ascorbic acid or D-erythorbic acid or an appropriate salt thereof, and isolating said product from the solution.
摘要翻译：或者在每一种情况下，由2-酮-L-古洛糖酸或2-酮-D-葡萄糖酸生产L-抗坏血酸或D-异抗坏血酸或其各自的钠，钾或钙盐的方法，钠，钾或钙盐，其包括将2-酮-L-古洛糖酸或2-酮-D-葡萄糖酸（游离酸或其钠，钾或钙盐）和热嗜酸性微生物在约30℃至约100℃的温度和约1至约6的pH下，在溶液中形成L-抗坏血酸或D-异抗坏血酸或其适当的盐，并将所述产品从解决方案。

6. 发明申请

US20030054523A1 Isoprenoid production 有权
公开(公告)号：US20030054523A1
公开(公告)日：2003-03-20
申请号：US09925388
申请日：2001-08-09
申请人： ROCHE VITAMINS INC.
发明人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
IPC分类号： C12P009/00 , C12N009/10 , C07H021/04 , C12N001/21 , C12P021/02 , C12N015/74
CPC分类号： C12N9/0004 , C12N9/1085 , C12N9/1205 , C12N9/88 , C12P23/00
摘要： The present invention relates to an isolated DNA sequence encoding an enzyme in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate. Vectors and plasmids including such DNA are also set forth. The invention also includes host cells transformed by such DNAs, or vectors or plasmids containing such DNAs. A process for the production of isoprenoids and carotenoids using such transformed host cells is also provided.

7. 发明申请

US20030190734A1 Isoprenoid production 有权
标题翻译：异戊二烯生产
公开(公告)号：US20030190734A1
公开(公告)日：2003-10-09
申请号：US10431846
申请日：2003-05-08
申请人： ROCHE VITAMINS, INC.
发明人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
IPC分类号： C12N009/02 , C12N009/10 , C12N009/12 , C07H021/04 , C12P021/02 , C12N005/06 , C12P023/00
CPC分类号： C12N9/0004 , C12N9/1085 , C12N9/1205 , C12N9/88 , C12P23/00
摘要： The present invention relates to an isolated DNA sequence encoding an enzyme in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate. Vectors and plasmids including such DNA are also set forth. The invention also includes host cells transformed by such DNAs, or vectors or plasmids containing such DNAs. A process for the production of isoprenoids and carotenoids using such transformed host cells is also provided.
摘要翻译：本发明涉及编码甲羟戊酸途径中的酶的分离DNA序列或从异戊烯焦磷酸至法呢基焦磷酸的途径。还阐述了包括这种DNA的载体和质粒。本发明还包括由这些DNA转化的宿主细胞，或含有这些DNA的载体或质粒。还提供了使用这种转化的宿主细胞产生类异戊二烯和类胡萝卜素的方法。

8. 发明申请

US20030096382A1 Production of biotin 审中-公开
标题翻译：生物素生产
公开(公告)号：US20030096382A1
公开(公告)日：2003-05-22
申请号：US09816733
申请日：2001-03-22
申请人： ROCHE VITAMINS INC.
发明人： Tatsuo Hoshino , Akira Asakura , Tatsuya Kiyasu , Yoshie Nagahashi
IPC分类号： C12P017/18 , C07D235/02
CPC分类号： C12N9/93 , C12N9/13 , C12P17/186
摘要： The present invention provides process for making biotin from desthiobiotin either contacting desthiobiotin with an enzyme reaction mixture containing bioB gene product and nifU gene product and/or nifS gene product and isolating the biotin or cultivating a microorganism transformed with DNA encoding the bioB gene product, nifU gene product and nifS gene product and isolating the biotin.
摘要翻译：本发明提供从脱硫生物素生产生物素的方法，该生物素可以将脱硫生物素与含有bioB基因产物和nifU基因产物和/或nifS基因产物的酶反应混合物接触，分离生物素或培养用编码bioB基因产物的DNA转化的微生物基因产物和nifS基因产物并分离生物素。

9. 发明申请

US20030049720A1 Process for producing carotenoids and biological materials useful therefor 有权
标题翻译：用于生产类胡萝卜素和生物材料的方法
公开(公告)号：US20030049720A1
公开(公告)日：2003-03-13
申请号：US09864894
申请日：2001-05-24
申请人： Roche Vitamins Inc.
发明人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
IPC分类号： C12P023/00 , C12N001/16 , C12N001/18 , C12N001/10
CPC分类号： C12N9/0006 , C12P23/00
摘要： A process for producing carotenoids, which involves cultivating a microorganism obtained by treating a parent microorganism that produces carotenoids under conditions that induce a reduction in alternative oxidase activity and selecting a microorganism with enhanced carotenoid productivity, a method for obtaining the microorganism with enhanced carotenoid productivity, and the microorganism itself.
摘要翻译：一种生产类胡萝卜素的方法，其涉及培养通过在诱导替代氧化酶活性降低的条件下处理产生类胡萝卜素的亲本微生物获得的微生物，并选择具有增强的类胡萝卜素生产力的微生物，获得具有增强的类胡萝卜素生产力的微生物的方法，和微生物本身。

10. 发明申请

US20020123109A1 Biotin biosynthetic genes 失效
标题翻译：生物素生物合成基因
公开(公告)号：US20020123109A1
公开(公告)日：2002-09-05
申请号：US10033078
申请日：2001-12-27
申请人： ROCHE VITAMINS INC.
发明人： Yasuhiro Furuichi , Tatsuo Hoshino , Hitoshi Kimura , Tatsuya Kiyasu , Yoshie Nagahashi
IPC分类号： C12P017/16 , C12N009/00 , C07H021/04 , C12P021/02 , C12N001/21
CPC分类号： C12N15/52 , A23L33/15 , A23V2002/00 , C12P17/185 , A23V2250/70
摘要： The present invention relates to the production process of biotin by fermentation using a genetically engineered microorganism, and DNA sequences and vectors to be used in such process.
摘要翻译：本发明涉及使用遗传工程微生物的发酵生物素的生产方法，以及用于该方法的DNA序列和载体。

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