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1. 发明授权

US5476930A Non-enzymatic ligation of oligonucleotides 失效
标题翻译：寡核苷酸的非酶连接
公开(公告)号：US5476930A
公开(公告)日：1995-12-19
申请号：US376688
申请日：1995-01-23
申请人： Robert L. Letsinger , Sergei M. Gryaznov
发明人： Robert L. Letsinger , Sergei M. Gryaznov
IPC分类号： C12N15/09 , A61K31/7088 , A61K48/00 , A61P31/12 , A61P35/00 , A61P43/00 , C07H21/00 , C07H21/04 , C12Q1/68 , C07H1/00
CPC分类号： C07H21/00 , C12Q1/6813
摘要： A process for non-enzymatic convalent ligation of two oligonucleotides having the structure shown below: ##STR1## The spontaneous reaction of these above oligonucleotides produces a thiophosphorylacetylamino linkage as shown below: ##STR2## When carried out in the presence of a template, this reaction gives 90% yields within 20 minutes at micromolar concentrations.
摘要翻译：具有以下结构的两种寡核苷酸的非酶共价连接的方法：这些上述寡核苷酸的自发反应产生如下所示的硫代磷酰基乙酰氨基键：当在模板存在下进行时，该反应在微摩尔浓度下在20分钟内产生90％产率。

2. 发明授权

US5646260A Oligonucleotides having modified internucleoside linkages 失效
标题翻译：具有修饰的核苷间键的寡核苷酸
公开(公告)号：US5646260A
公开(公告)日：1997-07-08
申请号：US471248
申请日：1995-06-06
申请人： Robert L. Letsinger , Sergei M. Gryaznov
发明人： Robert L. Letsinger , Sergei M. Gryaznov
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： Novel oligonucleotides, method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'-phosphorylated oligonucleotides are disclosed.
摘要翻译：公开了新的寡核苷酸，改进寡核苷酸的杂交性质的方法和制备3'-磷酸化寡核苷酸的新方法。

3. 发明授权

US5932718A Oligonucleotides having modified internucleoside linkages or terminal amino group 失效
标题翻译：具有修饰的核苷间键或末端氨基的寡核苷酸
公开(公告)号：US5932718A
公开(公告)日：1999-08-03
申请号：US659924
申请日：1996-06-07
申请人： Robert L. Letsinger , Sergei M. Gryaznov
发明人： Robert L. Letsinger , Sergei M. Gryaznov
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： Novel oligonucleotides, method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'-phosphorylated oligonucleotides.
摘要翻译：新型寡核苷酸，改善寡核苷酸杂交性质的方法和制备3'-磷酸化寡核苷酸的新方法。

4. 发明授权

US5648480A Process for making oligonucleotides having modified internucleoside linkages 失效
标题翻译：制备具有修饰的核苷间键的寡核苷酸的方法
公开(公告)号：US5648480A
公开(公告)日：1997-07-15
申请号：US467219
申请日：1995-06-06
申请人： Robert L. Letsinger , Sergei M. Gryaznov
发明人： Robert L. Letsinger , Sergei M. Gryaznov
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： Novel oligonucleotides, method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'-phosphorylated oligonucleotides are disclosed.
摘要翻译：公开了新的寡核苷酸，改进寡核苷酸的杂交性质的方法和制备3'-磷酸化寡核苷酸的新方法。

5. 发明授权

US5476925A Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups 失效
标题翻译：寡脱氧核糖核苷酸包括3'-氨基核苷 - 氨基磷酸酯键和末端3'-氨基
公开(公告)号：US5476925A
公开(公告)日：1995-12-19
申请号：US376291
申请日：1995-01-23
申请人： Robert L. Letsinger , Sergei M. Gryaznov
发明人： Robert L. Letsinger , Sergei M. Gryaznov
IPC分类号： C07H21/00 , C07H21/04
CPC分类号： C07H21/00
摘要： Novel oligonucleotides, method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'-phosphorylated oligonucleotides.
摘要翻译：新型寡核苷酸，改善寡核苷酸杂交性质的方法和制备3'-磷酸化寡核苷酸的新方法。

6. 发明授权

US06175002B1 Adaptor-based sequence analysis 有权
标题翻译：基于适配器的序列分析
公开(公告)号：US06175002B1
公开(公告)日：2001-01-16
申请号：US09225652
申请日：1999-01-05
申请人： Robert B. DuBridge , Glenn Albrecht , Sydney Brenner , Sergei M. Gryaznov , Sarah N. McCurdy
发明人： Robert B. DuBridge , Glenn Albrecht , Sydney Brenner , Sergei M. Gryaznov , Sarah N. McCurdy
IPC分类号： C07H2100
CPC分类号： C12Q1/6869 , C12Q2525/15 , C12Q2521/525 , C12Q2525/191
摘要： An improvement in adaptor-based sequence analysis is provided that addresses the problems created by self-ligation of target polynucleotides that have complementary ends. The improvement includes preparation of target polynucleotides with dephosphorylated 5′ strands and the use of adaptors having a 3′ blocking group. In a preferred embodiment, adaptors are ligated to target polynucleotides by a single strand, 3′ blocking groups are removed, the adjacent 5′ hydroxyl of the target polynucleotide is phosphorylated, and the ligation of the adaptor is completed by treatment with a ligase.
摘要翻译：提供了基于衔接子的序列分析的改进，其解决了具有互补末端的靶多核苷酸的自连接产生的问题。改进包括制备具有脱磷酸化的5'链的靶多核苷酸，以及使用具有3'阻断基团的衔接子。在优选的实施方案中，衔接子通过单链连接到靶多核苷酸，除去3'阻断基团，靶多核苷酸的相邻5'羟基被磷酸化，并且衔接子的连接通过用连接酶处理完成。

7. 发明授权

US06048974A Oligonucleotide clamps having diagnostic and therapeutic applications 失效
标题翻译：具有诊断和治疗应用的寡核苷酸夹具
公开(公告)号：US06048974A
公开(公告)日：2000-04-11
申请号：US70477
申请日：1998-04-30
申请人： Sergei M. Gryaznov , David H. Lloyd
发明人： Sergei M. Gryaznov , David H. Lloyd
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , C07H21/00 , C12Q1/6813 , C12N2310/152 , C12N2310/315 , C12N2310/318 , C12N2310/3341 , C12N2310/351 , C12N2310/3515
摘要： Compounds referred to herein as oligonucleotide clamps are provided that stably bind to target polynucleotides in a sequence-specific manner. The oligonucleotide clamps comprise one or more oligonucleotide moieties capable of specifically binding to a target polynucleotide and one or more pairs of binding moieties covalently linked to the oligonudeotide moieties. In accordance with the invention, upon annealing of the oligonucleotide moieties to the target polynucleotide, the binding moieties of a pair are brought into juxtaposition so that they form a stable covalent or non-covalent inkage or complex. The interaction of the binding moieties of the one or more pairs effectively clamps the specifically annealed oligonucleotide moieties to the target polynucleotide.
摘要翻译：提供本文中称为寡核苷酸钳位的化合物，其以序列特异性方式稳定地结合靶多核苷酸。寡核苷酸夹具包含能够特异性结合靶多核苷酸的一个或多个寡核苷酸部分和与寡核苷酸部分共价连接的一对或多对结合部分。根据本发明，当将寡核苷酸部分退火至靶多核苷酸时，一对的结合部分并列，使其形成稳定的共价或非共价的墨水或复合物。一个或多个对的结合部分的相互作用有效地将特异性退火的寡核苷酸部分夹持到靶多核苷酸。

8. 发明授权

US5830658A Convergent synthesis of branched and multiply connected macromolecular structures 失效
标题翻译：分支和多重连接的大分子结构的收敛合成
公开(公告)号：US5830658A
公开(公告)日：1998-11-03
申请号：US689856
申请日：1996-08-15
申请人： Sergei M. Gryaznov
发明人： Sergei M. Gryaznov
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/682
摘要： The invention provides methods and compositons for convergent synthesis of branched polymers useful as molecular probes. The invention also includes several novel branched polymeric structures particularly useful for detecting target polynucleotides. Branched polymers of the invention comprise at least two branches: at least one branch is a target binding moiety capable of specifically binding to a target molecule of interest and one or more branches are signal generation moities capable of directly or indirectly generating a detectable signal. In accordance with the method of the invention branched polymers are assembled from components having phosphorothioate groups and/or haloacyl- or haloalkylamino groups. The phosphorothioate and haloacyl- or haloalkylamino groups react rapidly and efficiently when brought into contact to form thiophosphorylacyl- or thiophosphorylalkylamino bridges which complete the assembly of a branched polymer. The method of the invention permits thorough purification and isolation of the components prior to final assembly. Incomplete branched polymers are readily separated from the desired product by standard means. The method of the invention permits the synthesis of several novel branched polymer configurations.
摘要翻译：本发明提供用于会聚合成可用作分子探针的支化聚合物的方法和组合物。本发明还包括特别可用于检测靶多核苷酸的几种新型支化聚合物结构。本发明的支化聚合物包含至少两个分支：至少一个分支是能够特异性结合目的靶分子的靶结合部分，一个或多个分支是能够直接或间接产生可检测信号的信号产生神经元。根据本发明的方法，支化聚合物由具有硫代磷酸酯基和/或卤代酰基 - 或卤代烷基氨基的组分组装。硫代磷酸酯和卤代酰基 - 或卤代烷基氨基当接触时反应迅速而有效地形成硫代磷酰基酰基或硫代磷酰烷基氨基桥，其完成支化聚合物的组装。本发明的方法允许在最终组装之前对组分进行彻底的纯化和分离。不完全的支化聚合物通过标准方法容易地与期望的产物分离。本发明的方法允许合成几种新的支化聚合物构型。

9. 发明授权

US5817795A Oligonucleotide clamps having diagnostic and therapeutic applications 失效
标题翻译：具有诊断和治疗应用的寡核苷酸夹具
公开(公告)号：US5817795A
公开(公告)日：1998-10-06
申请号：US713685
申请日：1996-09-17
申请人： Sergei M. Gryaznov , David H. Lloyd
发明人： Sergei M. Gryaznov , David H. Lloyd
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , C07H21/00 , C12Q1/6813 , C12N2310/152 , C12N2310/315 , C12N2310/318 , C12N2310/3341 , C12N2310/351 , C12N2310/3515
摘要： Compounds referred to herein as oligonucleotide clamps are provided that stably bind to target polynucleotides in a sequence-specific manner. The oligonucleotide clamps comprise one or more oligonucleotide moieties capable of specifically binding to a target polynucleotide and one or more pairs of binding moieties covalently linked to the oligonucleotide moieties. In accordance with the invention, upon annealing of the oligonucleotide moieties to the target polynucleotide, the binding moieties of a pair are brought into juxtaposition so that they form a stable covalent or non-covalent linkage or complex. The interaction of the binding moieties of the one or more pairs effectively clamps the specifically annealed oligonucleotide moieties to the target polynucleotide.
摘要翻译：提供本文中称为寡核苷酸钳位的化合物，其以序列特异性方式稳定地结合靶多核苷酸。寡核苷酸夹具包含能够特异性结合靶多核苷酸的一个或多个寡核苷酸部分和与寡核苷酸部分共价连接的一对或多对结合部分。根据本发明，当将寡核苷酸部分退火至靶多核苷酸时，将一对的结合部分并列，使其形成稳定的共价或非共价键或复合物。一个或多个对的结合部分的相互作用有效地将特异性退火的寡核苷酸部分夹持到靶多核苷酸。

10. 发明授权

US5571677A Convergent synthesis of branched and multiply connected macromomolecular structures 失效
标题翻译：分支和多重连接的大分子结构的收敛合成
公开(公告)号：US5571677A
公开(公告)日：1996-11-05
申请号：US455627
申请日：1995-05-31
申请人： Sergei M. Gryaznov
发明人： Sergei M. Gryaznov
IPC分类号： C12N15/09 , C07H1/00 , C07H21/00 , C07H21/04 , C08G81/00 , C12Q1/68 , C12P19/34 , C12Q1/70
CPC分类号： C07H21/00 , C12Q1/6813 , C12Q1/682
摘要： The invention provides methods and compositons for convergent synthesis of branched polymers useful as molecular probes. The invention also includes several novel branched polymeric structures particularly useful for detecting target polynucleotides. Branched polymers of the invention comprise at least two branches: at least one branch is a target binding moiety capable of specifically binding to a target molecule of interest and one or more branches are signal generation moities capable of directly or indirectly generating a detectable signal. In accordance with the method of the invention branched polymers are assembled from components having phosphorothioate groups and/or haloacyl- or haloalkylamino groups. The phosphorothioate and haloacyl- or haloalkylamino groups react rapidly and efficiently when brought into contact to form thiophosphorylacyl- or thiophosphorylalkylamino bridges which.complete the assembly of a branched polymer. The method of the invention permits thorough purification and isolation of the components prior to final assembly. Incomplete branched polymers are readily separated from the desired product by standard means. The method of the invention permits the synthesis of several novel branched polymer configurations.
摘要翻译：本发明提供用于会聚合成可用作分子探针的支化聚合物的方法和组合物。本发明还包括特别可用于检测靶多核苷酸的几种新型支化聚合物结构。本发明的支化聚合物包含至少两个分支：至少一个分支是能够特异性结合目的靶分子的靶结合部分，一个或多个分支是能够直接或间接产生可检测信号的信号产生神经元。根据本发明的方法，支化聚合物由具有硫代磷酸酯基和/或卤代酰基 - 或卤代烷基氨基的组分组装。硫代磷酸酯和卤代酰基 - 或卤代烷基氨基在接触时快速有效地反应形成硫代磷酰基酰基或硫代磷酰基烷基氨基桥，它们完成支化聚合物的组装。本发明的方法允许在最终组装之前对组分进行彻底的纯化和分离。不完全的支化聚合物通过标准方法容易地与期望的产物分离。本发明的方法允许合成几种新的支化聚合物构型。

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