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1. 发明申请

US20080032371A1 Immobilized TAL biocatalyst for production of para-hydroxycinnamic acid 有权
标题翻译：固定化的TAL生物催化剂用于生产对羟基肉桂酸
公开(公告)号：US20080032371A1
公开(公告)日：2008-02-07
申请号：US11485575
申请日：2006-07-12
申请人： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Robert J. Trotman , Tina K. Van Dyk
发明人： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Robert J. Trotman , Tina K. Van Dyk
IPC分类号： C12P7/24 , C12N11/10
CPC分类号： C12N11/04 , C12N9/88 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/588
摘要： TAL cell biocatalyst was immobilized in alginate cross-linked beads using low concentrations of glutaraldehyde. The biocatalyst beads have highly stable TAL activity and mechanical strength such that they withstand prolonged recycling in production of pHCA.
摘要翻译：使用低浓度戊二醛将TAL细胞生物催化剂固定在藻酸盐交联珠粒中。生物催化剂珠具有高度稳定的TAL活性和机械强度，使得它们在pHCA的生产中经受长时间的再循环。

2. 发明授权

US08569036B2 Immobilized TAL biocatalyst for production of para-hydroxycinnamic acid 有权
标题翻译：固定化的TAL生物催化剂用于生产对羟基肉桂酸
公开(公告)号：US08569036B2
公开(公告)日：2013-10-29
申请号：US11485575
申请日：2006-07-12
申请人： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Tina K. Van Dyk , Robert J. Trotman
发明人： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Tina K. Van Dyk , Robert J. Trotman
IPC分类号： C12N1/20 , C12N9/88 , C12P7/40 , C12P7/24 , C07H21/04
CPC分类号： C12N11/04 , C12N9/88 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/588
摘要： TAL cell biocatalyst was immobilized in alginate cross-linked beads using low concentrations of glutaraldehyde. The biocatalyst beads have highly stable TAL activity and mechanical strength such that they withstand prolonged recycling in production of pHCA.
摘要翻译：使用低浓度戊二醛将TAL细胞生物催化剂固定在藻酸盐交联珠粒中。生物催化剂珠具有高度稳定的TAL活性和机械强度，使得它们在pHCA的生产中经受长时间的再循环。

3. 发明申请

US20120328534A1 ENZYMATIC PERACID GENERATION FOR USE IN ORAL CARE PRODUCTS 有权
标题翻译：用于口腔护理产品的食用酸生产
公开(公告)号：US20120328534A1
公开(公告)日：2012-12-27
申请号：US13330261
申请日：2011-12-19
申请人： Lisa A. Butterick , Scott D. Cunningham , Robert DiCosimo , Kari A. Fosser , Tanja Maria Gruber , Sharon L. Haynie , Mark S. Payne , Pierre E. Rouviere , Hong Wang
发明人： Lisa A. Butterick , Scott D. Cunningham , Robert DiCosimo , Kari A. Fosser , Tanja Maria Gruber , Sharon L. Haynie , Mark S. Payne , Pierre E. Rouviere , Hong Wang
IPC分类号： A61K8/66 , A61K8/38 , A61K8/73 , C07K14/00 , A61P31/00 , A61Q11/00 , C12N9/96 , C07K7/08
CPC分类号： A61Q11/00 , A61K8/22 , A61K8/375 , A61K8/38 , A61K8/60 , A61K8/66
摘要： Disclosed herein are compositions and methods to treat an oral cavity surface with a peracid-based benefit agent. The peracid benefit agent can be use for oral surface bleaching, whitening, disinfecting, destaining, deodorizing, decreasing or removing biofilm, and combinations thereof. The peracid is enzymatically generated from a carboxylic acid ester substrate using a CE-7 carbohydrate esterase having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a peptidic component having affinity for an oral cavity surface, either directly or through an optional linker, may be used to target the perhydrolytic activity to the oral cavity surface.
摘要翻译：本文公开了用基于过酸的有益试剂治疗口腔表面的组合物和方法。过酸有益剂可用于口腔表面漂白，美白，消毒，脱色，除臭，减少或去除生物膜及其组合。在过氧源的存在下，使用具有过水解活性的CE-7碳水化合物酯酶（过水解酶），由羧酸酯底物酶促产生过酸。包含与直接或通过任选的接头对口腔表面具有亲和性的肽成分偶联的过水解酶的融合蛋白质可用于将过水解活性靶向口腔表面。

4. 发明授权

US08663616B2 Enzymatic peracid generation for use in oral care products 有权
标题翻译：酶促过酸生成用于口腔护理产品
公开(公告)号：US08663616B2
公开(公告)日：2014-03-04
申请号：US13330261
申请日：2011-12-19
申请人： Lisa A. Butterick , Scott D. Cunningham , Robert DiCosimo , Kari A. Fosser , Tanja Maria Gruber , Sharon L. Haynie , Mark S. Payne , Pierre E. Rouviere , Hong Wang
发明人： Lisa A. Butterick , Scott D. Cunningham , Robert DiCosimo , Kari A. Fosser , Tanja Maria Gruber , Sharon L. Haynie , Mark S. Payne , Pierre E. Rouviere , Hong Wang
IPC分类号： A61L9/00 , C12P7/40 , C12N9/96 , C12N9/18
CPC分类号： A61Q11/00 , A61K8/22 , A61K8/375 , A61K8/38 , A61K8/60 , A61K8/66
摘要： Disclosed herein are compositions and methods to treat an oral cavity surface with a peracid-based benefit agent. The peracid benefit agent can be use for oral surface bleaching, whitening, disinfecting, destaining, deodorizing, decreasing or removing biofilm, and combinations thereof. The peracid is enzymatically generated from a carboxylic acid ester substrate using a CE-7 carbohydrate esterase having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a peptidic component having affinity for an oral cavity surface, either directly or through an optional linker, may be used to target the perhydrolytic activity to the oral cavity surface.
摘要翻译：本文公开了用基于过酸的有益试剂治疗口腔表面的组合物和方法。过酸有益剂可用于口腔表面漂白，美白，消毒，脱色，除臭，减少或去除生物膜及其组合。在过氧源的存在下，使用具有过水解活性的CE-7碳水化合物酯酶（过水解酶），由羧酸酯底物酶促产生过酸。包含与直接或通过任选的接头对口腔表面具有亲和力的肽成分偶联的过水解酶的融合蛋白可用于将过水解活性靶向口腔表面。

5. 发明授权

US07452710B2 Process for the biological production of 1,3-propanediol with high titer 有权
标题翻译：高滴度生物制备1,3-丙二醇的方法
公开(公告)号：US07452710B2
公开(公告)日：2008-11-18
申请号：US11282993
申请日：2006-02-13
申请人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
发明人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
IPC分类号： C12N15/00 , C12N1/20 , C12N7/02
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
摘要翻译：本发明提供了从单一微生物中的可发酵碳源生物制备1,3-丙二醇的改进方法。在本发明的一个方面，通过使用用肺炎克雷伯杆菌dha调节基因dhaR或orfY，dhaT，orfX或ff转化的大肠杆菌来实现葡萄糖转化为1,3-丙二醇的改进方法， dhaB1，dhaB2，dhaB3和orfZ，所有这些基因排列在与野生型肺炎克雷伯菌中发现的相同的遗传组织中。在本发明的另一方面，使用含有编码G3PDH，G3P磷酸酶，脱水酶和脱水酶活化因子的重组大肠杆菌从与葡萄糖生产1,3-丙二醇的改进方法相比，使用含有编码G3PDH，G3P磷酸酶，脱水酶，脱水酶活化因子和1,3-丙二醇氧化还原酶（dhaT）的基因的重组大肠杆菌的方法。显着改善的方法依赖于在大肠杆菌中存在编码足以将3-羟基丙醛转化成1,3-丙二醇的非特异性催化活性的基因。

6. 发明授权

US07378261B2 Method for preparing para-hydroxystyrene by biocatalytic decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium 失效
标题翻译：在双相反应介质中通过对羟基肉桂酸的生物催化脱羧制备对羟基苯乙烯的方法
公开(公告)号：US07378261B2
公开(公告)日：2008-05-27
申请号：US10824581
申请日：2004-04-14
申请人： Arie Ben-Bassat , Sharon L. Haynie , David J. Lowe , Lisa L. Huang
发明人： Arie Ben-Bassat , Sharon L. Haynie , David J. Lowe , Lisa L. Huang
IPC分类号： C12P7/22 , C12N9/88 , C12N5/10 , C12N1/21 , C12N15/00 , C12N11/00 , C12P21/06
CPC分类号： C12P7/22 , C12P7/42
摘要： A biocatalytic method for preparing para-hydroxystyrene from para-hydroxycinnamic acid is described. The method uses an enzyme source having para-hydroxycinnamic acid decarboxylase activity to catalyze the decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium to produce para-hydroxystyrene, which is extracted into the organic phase of the biphasic reaction medium. The method results in a high yield of para-hydroxystyrene due to the decreased exposure of the enzyme source to the inhibitory product. The product is readily recovered from the extractant, or may be chemically derivatized directly in the extractant before recovery.
摘要翻译：描述了由对羟基肉桂酸制备对羟基苯乙烯的生物催化方法。该方法使用具有对羟基肉桂酸脱羧酶活性的酶源来催化双相反应介质中对羟基肉桂酸的脱羧作用，产生对羟基苯乙烯，将其提取到双相反应介质的有机相中。该方法由于酶源暴露于抑制产物而导致对羟基苯乙烯的高产率。该产物容易从萃取剂中回收，或者可以在回收之前在萃取剂中直接化学衍生化。

7. 发明授权

US5219662A Biocompatible polyurethanes by treatment with polyoxazoline block copolymers 失效
标题翻译：通过用聚恶唑啉嵌段共聚物处理生物相容的聚氨酯
公开(公告)号：US5219662A
公开(公告)日：1993-06-15
申请号：US704762
申请日：1991-05-23
申请人： Lisa C. Grimminger , Sharon L. Haynie , Mureo Kaku , Dotsevi Y. Sogah
发明人： Lisa C. Grimminger , Sharon L. Haynie , Mureo Kaku , Dotsevi Y. Sogah
IPC分类号： A61L27/18 , A61L33/06 , C07D263/10 , C08G73/02 , C08L79/02
CPC分类号： C07D263/10 , A61L27/18 , A61L33/068 , C08G73/0233 , C08L79/02 , Y10T428/31551
摘要： Polyurethane surfaces are made more biocompatible, particularly with blood, by treatment with a block copolymer of a fluorinated oxazoline and either 2-methyl- or 2-ethyl-2-oxazoline. Such polyurethanes are useful as vascular graft or artificial heart parts.
摘要翻译：通过用氟化恶唑啉和2-甲基 - 或2-乙基-2-恶唑啉的嵌段共聚物处理，使聚氨酯表面更具生物相容性，特别是与血液相容。这种聚氨酯可用作血管移植物或人造心脏部件。

8. 发明授权

US07067300B2 Process for the biological production of 1,3-propanediol with high titer 有权
公开(公告)号：US07067300B2
公开(公告)日：2006-06-27
申请号：US10277249
申请日：2002-10-21
申请人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Marshall Whited
发明人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Marshall Whited
IPC分类号： C12N1/20 , C12N1/14 , C07H21/02
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

9. 发明申请

US20090253192A1 PROCESS FOR THE BIOLOGICAL PRODUCTION OF 1,3-PROPANEDIOL WITH HIGH TITER 审中-公开
标题翻译：具有高TITER的1,3-丙二醇的生物生产工艺
公开(公告)号：US20090253192A1
公开(公告)日：2009-10-08
申请号：US12371745
申请日：2009-02-16
申请人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend
发明人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend
IPC分类号： C12N1/21
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
摘要翻译：本发明提供了从单一微生物中的可发酵碳源生物制备1,3-丙二醇的改进方法。在本发明的一个方面，通过使用用肺炎克雷伯杆菌dha调节基因dhaR或orfY，dhaT，orfX或ff转化的大肠杆菌来实现葡萄糖转化为1,3-丙二醇的改进方法， dhaB1，dhaB2，dhaB3和orfZ，所有这些基因排列在与野生型肺炎克雷伯菌中发现的相同的遗传组织中。在本发明的另一方面，使用含有编码G3PDH，G3P磷酸酶，脱水酶和脱水酶活化因子的重组大肠杆菌从与葡萄糖生产1,3-丙二醇的改进方法相比，使用含有编码G3PDH，G3P磷酸酶，脱水酶，脱水酶活化因子和1,3-丙二醇氧化还原酶（dhaT）的基因的重组大肠杆菌的方法。显着改善的方法依赖于在大肠杆菌中存在编码足以将3-羟基丙醛转化成1,3-丙二醇的非特异性催化活性的基因。

10. 发明授权

US07504250B2 Process for the biological production of 1,3-propanediol with high titer 有权
标题翻译：高滴度生物制备1,3-丙二醇的方法
公开(公告)号：US07504250B2
公开(公告)日：2009-03-17
申请号：US11282497
申请日：2006-01-16
申请人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
发明人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
IPC分类号： C12N1/20 , C12N9/00 , C12N9/02 , C12N15/00 , C12N9/04 , C12Q1/68 , C07H21/04 , C12Q1/00 , C12Q21/04
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
摘要翻译：本发明提供了从单一微生物中的可发酵碳源生物制备1,3-丙二醇的改进方法。在本发明的一个方面，通过使用用肺炎克雷伯杆菌dha调节基因dhaR或orfY，dhaT，orfX或ff转化的大肠杆菌来实现葡萄糖转化为1,3-丙二醇的改进方法， dhaB1，dhaB2，dhaB3和orfZ，所有这些基因排列在与野生型肺炎克雷伯菌中发现的相同的遗传组织中。在本发明的另一方面，使用含有编码G3PDH，G3P磷酸酶，脱水酶和脱水酶活化因子的重组大肠杆菌从与葡萄糖生产1,3-丙二醇的改进方法相比，使用含有编码G3PDH，G3P磷酸酶，脱水酶，脱水酶活化因子和1,3-丙二醇氧化还原酶（dhaT）的基因的重组大肠杆菌的方法。显着改善的方法依赖于在大肠杆菌中存在编码足以将3-羟基丙醛转化成1,3-丙二醇的非特异性催化活性的基因。

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