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1. 发明授权

US07452710B2 Process for the biological production of 1,3-propanediol with high titer 有权
标题翻译：高滴度生物制备1,3-丙二醇的方法
公开(公告)号：US07452710B2
公开(公告)日：2008-11-18
申请号：US11282993
申请日：2006-02-13
申请人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
发明人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
IPC分类号： C12N15/00 , C12N1/20 , C12N7/02
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
摘要翻译：本发明提供了从单一微生物中的可发酵碳源生物制备1,3-丙二醇的改进方法。在本发明的一个方面，通过使用用肺炎克雷伯杆菌dha调节基因dhaR或orfY，dhaT，orfX或ff转化的大肠杆菌来实现葡萄糖转化为1,3-丙二醇的改进方法， dhaB1，dhaB2，dhaB3和orfZ，所有这些基因排列在与野生型肺炎克雷伯菌中发现的相同的遗传组织中。在本发明的另一方面，使用含有编码G3PDH，G3P磷酸酶，脱水酶和脱水酶活化因子的重组大肠杆菌从与葡萄糖生产1,3-丙二醇的改进方法相比，使用含有编码G3PDH，G3P磷酸酶，脱水酶，脱水酶活化因子和1,3-丙二醇氧化还原酶（dhaT）的基因的重组大肠杆菌的方法。显着改善的方法依赖于在大肠杆菌中存在编码足以将3-羟基丙醛转化成1,3-丙二醇的非特异性催化活性的基因。

2. 发明授权

US07504250B2 Process for the biological production of 1,3-propanediol with high titer 有权
标题翻译：高滴度生物制备1,3-丙二醇的方法
公开(公告)号：US07504250B2
公开(公告)日：2009-03-17
申请号：US11282497
申请日：2006-01-16
申请人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
发明人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
IPC分类号： C12N1/20 , C12N9/00 , C12N9/02 , C12N15/00 , C12N9/04 , C12Q1/68 , C07H21/04 , C12Q1/00 , C12Q21/04
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
摘要翻译：本发明提供了从单一微生物中的可发酵碳源生物制备1,3-丙二醇的改进方法。在本发明的一个方面，通过使用用肺炎克雷伯杆菌dha调节基因dhaR或orfY，dhaT，orfX或ff转化的大肠杆菌来实现葡萄糖转化为1,3-丙二醇的改进方法， dhaB1，dhaB2，dhaB3和orfZ，所有这些基因排列在与野生型肺炎克雷伯菌中发现的相同的遗传组织中。在本发明的另一方面，使用含有编码G3PDH，G3P磷酸酶，脱水酶和脱水酶活化因子的重组大肠杆菌从与葡萄糖生产1,3-丙二醇的改进方法相比，使用含有编码G3PDH，G3P磷酸酶，脱水酶，脱水酶活化因子和1,3-丙二醇氧化还原酶（dhaT）的基因的重组大肠杆菌的方法。显着改善的方法依赖于在大肠杆菌中存在编码足以将3-羟基丙醛转化成1,3-丙二醇的非特异性催化活性的基因。

3. 发明授权

US06514733B1 Process for the biological production of 1,3-propanediol with high titer 有权
公开(公告)号：US06514733B1
公开(公告)日：2003-02-04
申请号：US09641652
申请日：2000-08-18
申请人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
发明人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend , Jeff P. Pucci , Gregory Whited
IPC分类号： C12P702
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

4. 发明申请

US20060148053A1 Process for the biological production of 1,3-propanediol with high titer 有权
公开(公告)号：US20060148053A1
公开(公告)日：2006-07-06
申请号：US11282497
申请日：2006-01-16
申请人： Mark Emptage , Sharon Haynie , Lisa Laffend , Jeff Pucci , Gregory Whited
发明人： Mark Emptage , Sharon Haynie , Lisa Laffend , Jeff Pucci , Gregory Whited
IPC分类号： C12P7/18 , C07H21/04 , C12P21/06 , C12N9/02 , C12N1/21 , C12N15/74
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

5. 发明申请

US20060121588A1 Process for the biological production of 1,3-propanediol with high titer 有权
公开(公告)号：US20060121588A1
公开(公告)日：2006-06-08
申请号：US11282993
申请日：2006-02-13
申请人： Mark Emptage , Sharon Haynie , Lisa Laffend , Jeff Pucci , Gregory Whited
发明人： Mark Emptage , Sharon Haynie , Lisa Laffend , Jeff Pucci , Gregory Whited
IPC分类号： C12P7/18 , C07H21/04 , C12P21/06 , C12N9/02 , C12N15/74 , C12N1/21
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

6. 发明授权

US06803218B1 Enzymes which dehydrate glycerol 有权
标题翻译：脱水甘油的酶
公开(公告)号：US06803218B1
公开(公告)日：2004-10-12
申请号：US09405692
申请日：1999-09-24
申请人： Markus Seyfried , Juergen Wiegel , Gregory Whited
发明人： Markus Seyfried , Juergen Wiegel , Gregory Whited
IPC分类号： C12P718
CPC分类号： C12N9/0004 , C12N9/88 , C12P7/18
摘要： The present invention relates to improved methods and reagents for the production of 1,3-propanediol. In particular, the present invention provides novel thermophilic organisms and thermostable enzymes cable of catalyzing the fermentation of glycerol to 1,3-propanediol. The present invention also relates to methods of isolating such thermophilic organisms, methods of cloning polynucleotides that encode such enzymes, polynucleotides encoding such enzymes, and methods of using such enzymes and organisms for the production of 1,3-propanediol.
摘要翻译：本发明涉及用于生产1,3-丙二醇的改进方法和试剂。特别地，本发明提供催化甘油发酵成1,3-丙二醇的新型嗜热生物体和热稳定酶电缆。本发明还涉及分离这种嗜热生物的方法，克隆编码这种酶的多核苷酸的方法，编码这种酶的多核苷酸，以及使用这些酶和生物体来生产1,3-丙二醇的方法。

7. 发明申请

US20070213550A1 Synthesis of cis-diols from aryl silanes 有权
标题翻译：从芳基硅烷合成顺式二醇
公开(公告)号：US20070213550A1
公开(公告)日：2007-09-13
申请号：US11646784
申请日：2006-12-28
申请人： Joseph Mcauliffe , Gregory Whited , Wyatt Smith
发明人： Joseph Mcauliffe , Gregory Whited , Wyatt Smith
IPC分类号： C07F7/08
CPC分类号： C12P9/00 , C07F7/081 , C07F7/0812 , C07F7/083 , C07F7/0896
摘要： The present invention is related to cis-diols and biological methods of producing cis-diols. The present invention further relates to processes for subsequently converting such silane cis-diols to the more stable acetonide derivatives, as well as a process for converting silane cis-diols to the corresponding catechols and the compounds produced thereby. The present invention also provides chemical methods for the conversion of said silane cis-diols and acetonide compounds to epoxy, saturated and otherwise modified derivatives. It is emphasized that this abstract is provided to comply with the rules requiring an abstract which will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that is will not be used to interpret or limit the scope or meaning of the claims.
摘要翻译：本发明涉及顺式二醇和生产顺式二醇的生物学方法。本发明还涉及随后将这种硅烷顺式二醇转化为更稳定的丙酮化合物衍生物的方法，以及将硅烷顺式二醇转化成相应的儿茶酚及其制备的化合物的方法。本发明还提供了将所述硅烷顺式二醇和丙酮化合物转化为环氧，饱和和其它改性衍生物的化学方法。要强调的是，该摘要被提供以符合要求摘要的规则，这将允许搜索者或其他读者快速确定技术公开的主题。提交的理解是不会用于解释或限制权利要求的范围或含义。

8. 发明申请

US20070213541A1 Synthesis of acetonides from aryl silanes 有权
标题翻译：从芳基硅烷合成丙酮化合物
公开(公告)号：US20070213541A1
公开(公告)日：2007-09-13
申请号：US11646945
申请日：2006-12-28
申请人： Joseph McAuliffe , Gregory Whited , Wyatt Smith
发明人： Joseph McAuliffe , Gregory Whited , Wyatt Smith
IPC分类号： C07D317/08 , C07F7/08
CPC分类号： C12P9/00 , C07F7/081 , C07F7/0812 , C07F7/083 , C07F7/0896
摘要： The present invention is related to cis-diols and biological methods of producing cis-diols. The present invention further relates to processes for subsequently converting such silane cis-diols to the more stable acetonide derivatives, as well as a process for converting silane cis-diols to the corresponding catechols and the compounds produced thereby. The present invention also provides chemical methods for the conversion of said silane cis-diols and acetonide compounds to epoxy, saturated and otherwise modified derivatives. It is emphasized that this abstract is provided to comply with the rules requiring an abstract which will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that is will not be used to interpret or limit the scope or meaning of the claims. 37 CFR 1.72(b).
摘要翻译：本发明涉及顺式二醇和生产顺式二醇的生物学方法。本发明还涉及随后将这种硅烷顺式二醇转化为更稳定的丙酮化合物衍生物的方法，以及将硅烷顺式二醇转化成相应的儿茶酚及其制备的化合物的方法。本发明还提供了将所述硅烷顺式二醇和丙酮化合物转化为环氧，饱和和其它改性衍生物的化学方法。要强调的是，该摘要被提供以符合要求摘要的规则，这将允许搜索者或其他读者快速确定技术公开的主题。提交的理解是不会用于解释或限制权利要求的范围或含义。 37 CFR 1.72（b）。

9. 发明申请

US20060003061A1 Browning agent 有权
标题翻译：褐变剂
公开(公告)号：US20060003061A1
公开(公告)日：2006-01-05
申请号：US10500945
申请日：2003-02-21
申请人： Matthew Boston , Gregory Whited
发明人： Matthew Boston , Gregory Whited
IPC分类号： A23L1/27
CPC分类号： A23L13/428 , A21D13/28 , A23L5/15 , A23L5/19 , A23L5/41 , A23L5/48 , A23L19/14 , A23L27/215 , A23P20/10 , A23V2002/00 , A23V2200/042 , A23V2250/60 , A23V2250/063
摘要： A browning agent for foodstuffs having at least two carbonyl groups is disclosed. A method for using the browning agent in or on a substrate is also disclosed. The browning agent may be coated onto foodstuffs such as biscuits, pizza, pie coverings or hash brown potatoes and heated by microwave or convection oven to induce browning.
摘要翻译：公开了具有至少两个羰基的食品的褐变剂。还公开了在基底中或基底上使用褐变剂的方法。褐变剂可以涂覆在诸如饼干，比萨饼，馅饼覆盖物或散布的褐色土豆等食品上，并通过微波或对流烘箱加热以诱导褐变。

10. 发明申请

US20050079617A1 Glucose transport mutants for production of biomaterial 审中-公开
标题翻译：用于生物材料生产的葡萄糖转运突变体
公开(公告)号：US20050079617A1
公开(公告)日：2005-04-14
申请号：US10728337
申请日：2003-12-03
申请人： Marguerite Cervin , Philippe Soucaille , Fernando Valle , Gregory Whited
发明人： Marguerite Cervin , Philippe Soucaille , Fernando Valle , Gregory Whited
IPC分类号： C12N1/20 , C12N15/70 , C12N15/74
CPC分类号： C12N15/70 , C12N1/20 , Y02E50/17
摘要： A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
摘要翻译：公开了一种用于将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS 细菌细胞的方法。包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡萄糖激酶的多核苷酸。

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