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1. 发明申请

US20060172278A1 Target activated microtransfer 有权
公开(公告)号：US20060172278A1
公开(公告)日：2006-08-03
申请号：US11202848
申请日：2005-08-12
申请人： Robert Bonner , Thomas Pohida , Michael Emmert-Buck , Michael Tangrea , Rodrigo Chuaqui
发明人： Robert Bonner , Thomas Pohida , Michael Emmert-Buck , Michael Tangrea , Rodrigo Chuaqui
IPC分类号： C12Q1/00 , G01N1/30 , C12M1/34
CPC分类号： G01N33/543 , G01N33/56966 , G01N2001/284 , Y10T428/2813
摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

2. 发明授权

US06743601B1 Non-contact laser capture microdissection 有权
标题翻译：非接触式激光捕获显微切割
公开(公告)号：US06743601B1
公开(公告)日：2004-06-01
申请号：US09456042
申请日：1999-12-06
申请人： Robert F. Bonner , Seth R Goldstein , Paul D. Smith , Thomas Pohida
发明人： Robert F. Bonner , Seth R Goldstein , Paul D. Smith , Thomas Pohida
IPC分类号： G01N130
CPC分类号： G01N1/2813 , G01N2001/284 , Y10T428/2813 , Y10T428/2839 , Y10T436/25375
摘要： An apparatus and process for the micro juxtaposition is set forth where a selectively activatable surface is maintained spaced apart from the tissue sample and juxtaposed to the tissue sample by activation. In the typical case, activation occurs by laser radiation with the material of the activatable surface thermally expanding and bringing about the desired micro juxtaposition. The disclosed micro juxtapositioning can cause locally and microscopically pressure on tissue sample, insertion to the tissue sample, or contact of an activated or prepared surface to the tissue sample.
摘要翻译：阐述了用于微并列的装置和方法，其中可选择性激活的表面保持与组织样本间隔开并且通过激活并置到组织样本。在典型情况下，通过激活辐射发生激活，其中可激活表面的材料热膨胀并产生所需的微并置。所公开的微并置可能导致组织样本的局部和显微压力，组织样本的插入或活化或制备的表面与组织样品的接触。

3. 发明申请

US20050074900A1 Microfluidic flow-through immunoassay for simultaneous detection of multiple proteins in a biological sample 审中-公开
标题翻译：用于同时检测生物样品中多种蛋白质的微流控流通免疫测定
公开(公告)号：US20050074900A1
公开(公告)日：2005-04-07
申请号：US10882188
申请日：2004-07-02
申请人： Nicole Morgan , Michael Gaitan , Laurie Locascio , Terry Phillips , Thomas Pohida , Paul Smith
发明人： Nicole Morgan , Michael Gaitan , Laurie Locascio , Terry Phillips , Thomas Pohida , Paul Smith
IPC分类号： G01N33/558
CPC分类号： G01N33/558
摘要： Described are microfluidic devices for, preferably, high-throughput, multi-analyte, affinity capture and detection of affinity-bindable analytes in biological fluids. Particularly, the devices can be used for immunoassays of biological fluids using multiple antibodies for capture and detection of multiple analytes, including proteins. The devices can be used for the simultaneous isolation and quantization of multiple proteins from microliter samples of biological fluids. Also described are methods for detecting and, optionally, quantifying, affinity-bindable analytes in biological fluids using these devices.
摘要翻译：描述的是微流体装置，优选地，高通量，多分析物，亲和捕获和生物流体中的亲和力结合分析物的检测。特别地，这些装置可以用于使用多种抗体捕获和检测多种分析物（包括蛋白质）的生物流体的免疫测定。该装置可用于从微生物液体样品中同时分离和量化多种蛋白质。还描述了使用这些装置检测和任选地定量生物流体中的亲和力结合分析物的方法。

4. 发明授权

US06783734B1 Convex geometry adhesive film system for laser capture microdissection 失效
标题翻译：凸形几何胶片系统用于激光捕获显微切割
公开(公告)号：US06783734B1
公开(公告)日：2004-08-31
申请号：US09387810
申请日：1999-09-01
申请人： Seth R. Goldstein , Robert F. Bonner , Paul D. Smith , John Peterson , Thomas Pohida
发明人： Seth R. Goldstein , Robert F. Bonner , Paul D. Smith , John Peterson , Thomas Pohida
IPC分类号： B01L1100
CPC分类号： G02B21/34 , G01N1/2813 , G01N2001/2833 , G01N2001/284 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A tissue sample is conventionally visualized in a microscope. A selectively activated convex surface is provided, preferably at the distal end of a rod. This selectively activated convex surface when activated, typically with a laser through an optic light path in the microscope, provides the activated region with adhesive properties. At least one portion of the tissue sample which is to be extracted is identified. This identified portion is contacted with a portion of the selectively activated convex surface on the end of the rod. When the convex surface is activated, typically by exposure to laser light in the footprint of the desired sample, an adhesive transfer surface on the selectively activated convex surface is provided which adheres to the desired cells in the footprint of the desired sample. Thereafter, the adhesive transfer surface is separated from the remainder of the tissue sample while maintaining adhesion with the desired cells. Thus the desired portion of the tissue sample is extracted. The disclosed selectively activated convex surface is preferably utilized to collect desired tissue samples at more than one location on the same slide or from different slides. The collected tissue samples can thereafter be inspected if desired, as collected on the convex surface, and then liberated—as by dissolving the proteins of the samples. This can effectively concentrate rarely occurring cells in order to obtain enough pure material for analysis. A rod having a convex surface with the selectively activated material is set forth as a staple for use with the apparatus and process. Preferred shapes for the convex surface are disclosed as well as a method for coating rods with a resultant rod article.
摘要翻译：传统的组织样本在显微镜下可视化。提供选择性活化的凸表面，优选地在杆的远端处。当被激活时，这种选择性活化的凸表面通常通过在显微镜中通过光学光路的激光器提供激活区域的粘合性质。识别要提取的组织样品的至少一部分。该识别部分与杆的端部上的选择性活化的凸面的一部分接触。当凸表面被激活时，通常通过暴露于所需样品的覆盖区域中的激光，在选择性活化的凸表面上提供粘合剂转移表面，该粘合剂转移表面在期望样品的覆盖区中粘附到期望的细胞。此后，粘合剂转移表面与组织样品的其余部分分离，同时保持与所需细胞的粘附。因此，提取组织样本的期望部分。所公开的选择性活化的凸表面优选用于在相同载片上或不同载玻片上的多于一个位置处收集所需的组织样品。此后，如果需要，收集的组织样品可以如在凸表面上收集的那样被检查，然后通过溶解样品的蛋白质来释放。这可以有效地集中很少发生的细胞，以获得足够的纯物质进行分析。具有带有选择性活化材料的凸表面的杆被列为与装置和工艺一起使用的订书钉。公开了用于凸表面的优选形状以及用所述棒制品涂覆棒的方法。

5. 发明授权

US06720191B1 Mechanical handling systems for laser capture microdissection 失效
标题翻译：用于激光捕获显微切割的机械处理系统
公开(公告)号：US06720191B1
公开(公告)日：2004-04-13
申请号：US09601559
申请日：2000-10-30
申请人： Seth R. Goldstein , Robert F. Bonner , Paul D. Smith , John Peterson , Thomas Pohida
发明人： Seth R. Goldstein , Robert F. Bonner , Paul D. Smith , John Peterson , Thomas Pohida
IPC分类号： G01N100
CPC分类号： G01N1/2813 , G01N35/00009 , G01N2001/282 , G01N2001/284 , Y10T436/11 , Y10T436/25
摘要： A method and apparatus of gathering by LCM identified cellular material from randorn locations on a tissue sample to designated locations on a transporting substrate enables convenient further processing. A transporting substrate has an identified mapped location for receiving identified cellular material. At least a segment of a selectively activatable coating is placed on the side of the transporting substrate in apposition to the tissue sample at the mapped location. The transporting substrate and sample are relatively moved to place the selectively activated coating at the mapped location in apposition to identified cellular material of the tissue sample which is to be extracted. Thereafter, the selectively activatable coating is activated and impressed or impressed and activated to form an adhesive region on the transporting substrate for adhering to the identified cellular material. Upon removal of the transporting substrate from the tissue sample, identified cellular material adheres to the transporting substrate at the mapped location.
摘要翻译：通过LCM聚集的方法和装置将组织样本上的多个位置的细胞材料鉴定到运输基底上的指定位置，便于进一步处理。传输基板具有用于接收所识别的细胞材料的识别的映射位置。至少一段可选择性活化的涂层被放置在运送基片的侧面上，以与所述组织样本在所述位置相对应。运输基质和样品被相对移动以将选择性活化的涂层置于映射位置处，以与待提取的组织样品的鉴定的细胞材料相关联。此后，可选择性活化的涂层被活化并印刷或印刷并活化，以在传送基材上形成粘合区域，以粘附到所鉴定的多孔材料上。当从组织样品中移走运送基质时，鉴定的细胞材料在映射位置附着于运输基质。

6. 发明授权

US6100051A Method utilizing convex geometry for laser capture microdissection 失效
标题翻译：用于激光捕获显微切割的凸几何的方法
公开(公告)号：US6100051A
公开(公告)日：2000-08-08
申请号：US883821
申请日：1997-06-27
申请人： Seth R. Goldstein , Robert F. Bonner , Paul D. Smith , John Peterson , Thomas Pohida
发明人： Seth R. Goldstein , Robert F. Bonner , Paul D. Smith , John Peterson , Thomas Pohida
IPC分类号： G01N1/28 , G02B21/34 , C12Q1/08
CPC分类号： G02B21/34 , G01N1/2813 , G01N2001/2833 , G01N2001/284 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A process of microdissection where a tissue sample is conventionally visualized in a microscope. A selectively activatable convex surface is provided, preferably on the periphery at the distal end of a rod. This selectively activatable convex surface when locally activated, typically with a laser through an optic light path in the microscope, provides the activated region with adhesive properties. The tissue sample has at least one portion, which is to be extracted is identified. This identified portion is contacted with a portion of the selectively activatable convex surface on the periphery of the rod. When the convex surface is locally activated, typically by exposure to laser light in the footprint of the desired portion, an adhesive transfer surface on the selectively activatable convex surface is activated which adheres to the desired cells in the footprint of the desired portion. Thereafter, the adhesive transfer surface is separated from the remainder of the tissue sample while maintaining adhesion with the portion of the sample. Thus the desired portion of the tissue sample is extracted. The disclosed selectively activatable convex surface is preferably utilized to collect desired tissue samples at more than one location on the same slide or from different slides. A rod having a convex surface with the selectively activatable material is set forth as a staple for use with the apparatus and process. Preferred shapes for the convex surface are disclosed as well as a method for coating rods.
摘要翻译：显微切割的过程，其中组织样品通常在显微镜中可视化。优选地，在棒的远端的周边上设置有选择性激活的凸面。当局部激活时，这种选择性激活的凸表面通常在显微镜中通过光学光路通过激光提供具有粘合性质的激活区域。组织样本具有至少一部分要被提取的部分。该识别部分与杆的周边上的可选择性激活的凸面的一部分接触。当凸表面被局部激活时，通常通过暴露在所需部分的覆盖区内的激光，激活可选择性激活的凸表面上的粘合剂转移表面，该粘合剂转移表面在期望部分的覆盖区中粘附到期望的电池。此后，粘合剂转移表面与组织样品的其余部分分离，同时保持与样品部分的粘附。因此，提取组织样本的期望部分。所公开的可选择激活的凸表面优选用于在相同载片上或不同载玻片上的多于一个位置收集所需的组织样本。具有具有可选择性活化材料的凸起表面的杆被设置为与装置和工艺一起使用的订书钉。公开了用于凸表面的优选形状以及用于涂覆棒的方法。

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