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    • 1. 发明授权
    • Convex geometry adhesive film system for laser capture microdissection
    • 凸形几何胶片系统用于激光捕获显微切割
    • US06783734B1
    • 2004-08-31
    • US09387810
    • 1999-09-01
    • Seth R. GoldsteinRobert F. BonnerPaul D. SmithJohn PetersonThomas Pohida
    • Seth R. GoldsteinRobert F. BonnerPaul D. SmithJohn PetersonThomas Pohida
    • B01L1100
    • G02B21/34G01N1/2813G01N2001/2833G01N2001/284Y10T436/25Y10T436/25125Y10T436/25375
    • A tissue sample is conventionally visualized in a microscope. A selectively activated convex surface is provided, preferably at the distal end of a rod. This selectively activated convex surface when activated, typically with a laser through an optic light path in the microscope, provides the activated region with adhesive properties. At least one portion of the tissue sample which is to be extracted is identified. This identified portion is contacted with a portion of the selectively activated convex surface on the end of the rod. When the convex surface is activated, typically by exposure to laser light in the footprint of the desired sample, an adhesive transfer surface on the selectively activated convex surface is provided which adheres to the desired cells in the footprint of the desired sample. Thereafter, the adhesive transfer surface is separated from the remainder of the tissue sample while maintaining adhesion with the desired cells. Thus the desired portion of the tissue sample is extracted. The disclosed selectively activated convex surface is preferably utilized to collect desired tissue samples at more than one location on the same slide or from different slides. The collected tissue samples can thereafter be inspected if desired, as collected on the convex surface, and then liberated—as by dissolving the proteins of the samples. This can effectively concentrate rarely occurring cells in order to obtain enough pure material for analysis. A rod having a convex surface with the selectively activated material is set forth as a staple for use with the apparatus and process. Preferred shapes for the convex surface are disclosed as well as a method for coating rods with a resultant rod article.
    • 传统的组织样本在显微镜下可视化。 提供选择性活化的凸表面,优选地在杆的远端处。 当被激活时,这种选择性活化的凸表面通常通过在显微镜中通过光学光路的激光器提供激活区域的粘合性质。 识别要提取的组织样品的至少一部分。 该识别部分与杆的端部上的选择性活化的凸面的一部分接触。 当凸表面被激活时,通常通过暴露于所需样品的覆盖区域中的激光,在选择性活化的凸表面上提供粘合剂转移表面,该粘合剂转移表面在期望样品的覆盖区中粘附到期望的细胞。 此后,粘合剂转移表面与组织样品的其余部分分离,同时保持与所需细胞的粘附。 因此,提取组织样本的期望部分。 所公开的选择性活化的凸表面优选用于在相同载片上或不同载玻片上的多于一个位置处收集所需的组织样品。 此后,如果需要,收集的组织样品可以如在凸表面上收集的那样被检查,然后通过溶解样品的蛋白质来释放。 这可以有效地集中很少发生的细胞,以获得足够的纯物质进行分析。 具有带有选择性活化材料的凸表面的杆被列为与装置和工艺一起使用的订书钉。 公开了用于凸表面的优选形状以及用所述棒制品涂覆棒的方法。
    • 2. 发明授权
    • Mechanical handling systems for laser capture microdissection
    • 用于激光捕获显微切割的机械处理系统
    • US06720191B1
    • 2004-04-13
    • US09601559
    • 2000-10-30
    • Seth R. GoldsteinRobert F. BonnerPaul D. SmithJohn PetersonThomas Pohida
    • Seth R. GoldsteinRobert F. BonnerPaul D. SmithJohn PetersonThomas Pohida
    • G01N100
    • G01N1/2813G01N35/00009G01N2001/282G01N2001/284Y10T436/11Y10T436/25
    • A method and apparatus of gathering by LCM identified cellular material from randorn locations on a tissue sample to designated locations on a transporting substrate enables convenient further processing. A transporting substrate has an identified mapped location for receiving identified cellular material. At least a segment of a selectively activatable coating is placed on the side of the transporting substrate in apposition to the tissue sample at the mapped location. The transporting substrate and sample are relatively moved to place the selectively activated coating at the mapped location in apposition to identified cellular material of the tissue sample which is to be extracted. Thereafter, the selectively activatable coating is activated and impressed or impressed and activated to form an adhesive region on the transporting substrate for adhering to the identified cellular material. Upon removal of the transporting substrate from the tissue sample, identified cellular material adheres to the transporting substrate at the mapped location.
    • 通过LCM聚集的方法和装置将组织样本上的多个位置的细胞材料鉴定到运输基底上的指定位置,便于进一步处理。 传输基板具有用于接收所识别的细胞材料的识别的映射位置。 至少一段可选择性活化的涂层被放置在运送基片的侧面上,以与所述组织样本在所述位置相对应。 运输基质和样品被相对移动以将选择性活化的涂层置于映射位置处,以与待提取的组织样品的鉴定的细胞材料相关联。 此后,可选择性活化的涂层被活化并印刷或印刷并活化,以在传送基材上形成粘合区域,以粘附到所鉴定的多孔材料上。 当从组织样品中移走运送基质时,鉴定的细胞材料在映射位置附着于运输基质。
    • 3. 发明授权
    • Method utilizing convex geometry for laser capture microdissection
    • 用于激光捕获显微切割的凸几何的方法
    • US6100051A
    • 2000-08-08
    • US883821
    • 1997-06-27
    • Seth R. GoldsteinRobert F. BonnerPaul D. SmithJohn PetersonThomas Pohida
    • Seth R. GoldsteinRobert F. BonnerPaul D. SmithJohn PetersonThomas Pohida
    • G01N1/28G02B21/34C12Q1/08
    • G02B21/34G01N1/2813G01N2001/2833G01N2001/284Y10T436/25Y10T436/25125Y10T436/25375
    • A process of microdissection where a tissue sample is conventionally visualized in a microscope. A selectively activatable convex surface is provided, preferably on the periphery at the distal end of a rod. This selectively activatable convex surface when locally activated, typically with a laser through an optic light path in the microscope, provides the activated region with adhesive properties. The tissue sample has at least one portion, which is to be extracted is identified. This identified portion is contacted with a portion of the selectively activatable convex surface on the periphery of the rod. When the convex surface is locally activated, typically by exposure to laser light in the footprint of the desired portion, an adhesive transfer surface on the selectively activatable convex surface is activated which adheres to the desired cells in the footprint of the desired portion. Thereafter, the adhesive transfer surface is separated from the remainder of the tissue sample while maintaining adhesion with the portion of the sample. Thus the desired portion of the tissue sample is extracted. The disclosed selectively activatable convex surface is preferably utilized to collect desired tissue samples at more than one location on the same slide or from different slides. A rod having a convex surface with the selectively activatable material is set forth as a staple for use with the apparatus and process. Preferred shapes for the convex surface are disclosed as well as a method for coating rods.
    • 显微切割的过程,其中组织样品通常在显微镜中可视化。 优选地,在棒的远端的周边上设置有选择性激活的凸面。 当局部激活时,这种选择性激活的凸表面通常在显微镜中通过光学光路通过激光提供具有粘合性质的激活区域。 组织样本具有至少一部分要被提取的部分。 该识别部分与杆的周边上的可选择性激活的凸面的一部分接触。 当凸表面被局部激活时,通常通过暴露在所需部分的覆盖区内的激光,激活可选择性激活的凸表面上的粘合剂转移表面,该粘合剂转移表面在期望部分的覆盖区中粘附到期望的电池。 此后,粘合剂转移表面与组织样品的其余部分分离,同时保持与样品部分的粘附。 因此,提取组织样本的期望部分。 所公开的可选择激活的凸表面优选用于在相同载片上或不同载玻片上的多于一个位置收集所需的组织样本。 具有具有可选择性活化材料的凸起表面的杆被设置为与装置和工艺一起使用的订书钉。 公开了用于凸表面的优选形状以及用于涂覆棒的方法。
    • 6. 发明申请
    • DISTORTION OF RASTER AND VECTOR ARTWORK
    • 镭射和矢量作品的失败
    • US20130162681A1
    • 2013-06-27
    • US12124059
    • 2008-05-20
    • John Peterson
    • John Peterson
    • G09G5/00
    • G06T11/60G06T3/0093G06T11/40G06T15/04G06T15/30
    • Systems and techniques to apply an image distortion to two image objects of different graphic types. In general, in one implementation, the technique includes: receiving an image distortion description to be applied to an image portion including a vector graphic and a raster graphic, the raster graphic being distortable separate from the vector graphic, applying the image distortion description to the vector graphic to produce a distorted vector graphic, and applying the image distortion description to the raster graphic to produce a distorted raster graphic, the distorted vector graphic and the distorted raster graphic together forming a distorted image portion.
    • 将图像失真应用于不同图形类型的两个图像对象的系统和技术。 通常,在一个实现中,该技术包括:接收要应用于包括矢量图形和光栅图形的图像部分的图像失真描述,该光栅图形与矢量图形分离可变形,将图像失真描述应用于 向量图形以产生失真的矢量图形,并将图像失真描述应用于光栅图形,以产生失真的光栅图形,失真的矢量图形和失真的光栅图形,一起形成失真的图像部分。
    • 10. 发明申请
    • SYSTEM AND METHOD FOR ASSISTING IN THE REFILLING OF AGRICULTURAL VEHICLES
    • 协助农机改装的制度和方法
    • US20110084851A1
    • 2011-04-14
    • US12577508
    • 2009-10-12
    • John PetersonDave Lovell
    • John PetersonDave Lovell
    • G08G1/00G01C21/00
    • A01M7/0089A01B69/008
    • A system and method for directing when and where an agricultural vehicle should refill a container thereof may comprise a sensor for detecting an amount of material in the container, a location determining device, and a processing device communicably coupled with the sensor and the location determining device, as well as a display, a speaker, and/or an automated steering system. The processing device may use information regarding the amount of material in the container, the location of the agricultural vehicle, and a variety of other factors to determine when the container needs to be refilled and to determine a refill location at which the agricultural vehicle should refill. The processing device may send these refill instructions to the display, the speaker, and/or the automated steering system. The processing device may also alert a refill vehicle to travel to the determined refill location, if necessary.
    • 用于指示农用车辆何时何地重新装满其容器的系统和方法可以包括用于检测容器中的材料量的传感器,位置确定装置和与传感器和位置确定装置可通信地耦合的处理装置 ,以及显示器,扬声器和/或自动转向系统。 处理装置可以使用关于容器中的材料量,农用车辆的位置以及各种其他因素的信息来确定何时需要重新填充容器并且确定农用车辆应该再填充的再填充位置 。 处理装置可以将这些再填充指令发送到显示器,扬声器和/或自动转向系统。 如果需要,处理装置还可以警告再充填车辆行进到确定的再填充位置。