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JPS58192827A Novel substance with action of growing nerves 失效
标题翻译：新的物质与增长神经的行动
公开(公告)号：JPS58192827A
公开(公告)日：1983-11-10
申请号：JP7560282
申请日：1982-05-06
申请人： Rikagaku Kenkyusho , Hiroshi Saito
发明人： SAITOU HIROSHI , NISHIYAMA NOBUYOSHI , FURUKAWA SHIYOUEI , YOUROU TAKESHI , KAMIYAMA TAKASHI
IPC分类号： C07K14/52 , A61K35/37 , A61K35/55 , A61K38/00 , A61P25/00 , A61P43/00 , C07K14/435 , C07K14/48
摘要： NEW MATERIAL:A substance active as nerve-growing factor (NGF); a white powder; soluble in water; molecular weight; 12400 and 11600 (by the SDS-gel electrophoresis), 18000 and 12600 (by the gel filtration); aminoacid analyses: shown in the table; showing no immunological cross-over reaction with mouse β- NGF antiserum prepared from rabbit; the concentration giving the maximum growth of nerve fiber in egg ganglions: 1.0mg/ml.
USE: A substance growing nerves.
PREPARATION: Gradulas submandibularis or grandulas protstatica of Suncus murinus are homogenized in water, centrifuged and the surpernatant is freeze dried. The product is dissolved in a buffer solution and fractionated by means of column chromatography. Each fraction is examined on its absorption and NGF activity and the active fractions are dialyzed with a buffer solution and centrifuged. The resultant supernatant is controlled in pH and subjected to column chromatography again, eluted under salt concentration gredient. The fractions showing NGF activity are concentrated, further subjected to column chromatography to isolate and purify the substance with NGF activity.
COPYRIGHT: (C)1983,JPO&Japio
摘要翻译：新材料：作为神经生长因子（NGF）活性物质; 白色粉末; 溶于水; 分子量 12400和11600（通过SDS-凝胶电泳），18000和12600（通过凝胶过滤）; 氨基酸分析：如表所示; 显示与兔制备的小鼠β-NGF抗血清无免疫学交叉反应; 给予蛋神经节神经纤维最大生长的浓度为1.0mg / ml。使用：一种物质生长的神经。制备方法：将水：ulas ulas sub is or or static static us us us us us。。。。。。。。。。。。。。。。，，，。。。。。。。将产物溶于缓冲溶液中，并通过柱色谱分离。检查每个级分的吸收和NGF活性，并用缓冲液透析活性级分并离心。将所得上清液控制在pH下，再次进行柱层析，在盐浓度下洗脱。浓缩显示NGF活性的级分，进一步进行柱色谱分离并纯化具有NGF活性的物质。

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