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1. 发明授权

US5106762A Ligand-label conjugates which contain polyoxoanions of sulfur or phosphorus 失效
标题翻译：包含硫或多磷酸多糖的标签结合物
公开(公告)号：US5106762A
公开(公告)日：1992-04-21
申请号：US512272
申请日：1990-04-20
申请人： Reinhard Bredehorst , Frances S. Ligler , Anne W. Kusterbeck , Gregory A. Wemhoff , Carl-Wilhelm Vogel
发明人： Reinhard Bredehorst , Frances S. Ligler , Anne W. Kusterbeck , Gregory A. Wemhoff , Carl-Wilhelm Vogel
IPC分类号： C07K14/62 , G01N33/533
CPC分类号： C07K14/62 , G01N33/533
摘要： Ligand-label conjugates which are an oligopeptide of 5 to 100 amino acid residues, bonded to a ligand or receptor, which contain a plurality of chemiluminescent or fluorescent labels and a plurality of polyoxoanions of sulfur or phosphorus are useful for immunoassays. Such conjugates are hydrophilic and exhibit very low nonspecific binding, thereby significantly increasing the signal to background ratio in immunoassays.
摘要翻译：与配体或受体结合的5至100个氨基酸残基的寡肽的配体 - 标记缀合物，其含有多个化学发光或荧光标记和多个硫或磷的多氧阴离子可用于免疫测定。这种缀合物是亲水性的并且表现出非常低的非特异性结合，从而显着增加免疫测定中的信号与背景比。

2. 发明授权

US06245296B1 Flow immunosensor apparatus 失效
标题翻译：流量免疫传感器
公开(公告)号：US06245296B1
公开(公告)日：2001-06-12
申请号：US07860965
申请日：1992-03-31
申请人： Frances S. Ligler , Bruce P. Gaber , Anne W. Kusterbeck , Gregory A. Wemhoff
发明人： Frances S. Ligler , Bruce P. Gaber , Anne W. Kusterbeck , Gregory A. Wemhoff
IPC分类号： G01N3348
CPC分类号： G01N33/54366 , G01N33/53 , G01N33/54306 , Y10S435/80 , Y10S435/804 , Y10S435/961 , Y10S435/968
摘要： Target moiety is detected by (a) providing an antibody specific to the target, (b) saturating the binding sites of the antibody with a labelled form of the target, (c) flowing a liquid containing the target past the saturated antibody, thereby (d) allowing the target to displace the labelled antigen, and (e) detecting the displaced labelled antigen with a detector for the label. This assay is performed by a flow immunosensor comprising: a liquid flowing through the immunosensor for moving a sample through the immunosensor; a sample receiver for introducing the sample to the liquid stream; a flow controller for moving the liquid stream through the immunosensor; an exchanger connected to the sample receiving area in which the sample is brought in contact with labelled antigens of the target and where any target present is allowed to displace the labelled antigen; a detection apparatus connected to the exchanger for detecting any labelled antigen which is released; and a disposal means connected to the detection apparatus for disposing of or collecting of waste coming from the detection apparatus.
摘要翻译：通过（a）提供对靶特异性的抗体，（b）用靶标的标记形式使抗体的结合位点饱和，（c）使含有靶标的液体流过饱和抗体，从而（ d）允许目标物置换标记的抗原，和（e）用标记的检测器检测置换的标记的抗原。该测定由流动免疫传感器进行，包括：流过免疫传感器的液体，用于使样品移动通过免疫传感器; 用于将样品引入液体流的样品接收器; 用于使液体流通过免疫传感器的流量控制器; 连接到样品接收区域的交换器，其中样品与靶标的标记抗原接触，并且允许存在的任何靶标置换标记的抗原; 连接到交换器的检测装置，用于检测被释放的任何标记的抗原; 以及与检测装置连接的处置装置，用于处置或收集来自检测装置的废物。

3. 发明授权

US5183740A Flow immunosensor method and apparatus 失效
标题翻译：流动免疫传感器方法和装置
公开(公告)号：US5183740A
公开(公告)日：1993-02-02
申请号：US486024
申请日：1990-02-23
申请人： Frances S. Ligler , Gaber Bruce P. , Anne W. Kusterbeck , Gregory A. Wemhoff
发明人： Frances S. Ligler , Gaber Bruce P. , Anne W. Kusterbeck , Gregory A. Wemhoff
IPC分类号： G01N33/536 , G01N33/53 , G01N33/543 , G01N35/02
CPC分类号： G01N33/54366 , G01N33/53 , G01N33/54306 , Y10S435/80 , Y10S435/804 , Y10S435/961 , Y10S435/968
摘要： A method of detecting a target moiety comprising the steps of (a) providing an antibody specific to the target, (b) saturating the binding sites of the antibody with a labelled form of the target, (c) flowing a liquid containing the target past the saturated antibody, thereby (d) allowing the target to displace the labelled antigen, and (e) detecting the displaced labelled antigen with a detector for the label.Also, the invention is accomplished by a flow immunosensor comprising: a liquid flowing through the immunosensor for moving a sample through the immunosensor; a receiving means for introducing the sample to the liquid stream; a flow control means for moving the liquid stream through the immunosensor; an exchanger connected to the sample receiving means in which the sample is brought in contact with labelled antigens of the target and whereby any target present is allowed to displace the labelled antigen; a detection apparatus connected to the exchanger for detecting any labelled antigen which is released; and a disposal means connected to the detection apparatus for disposing of or collecting of waste coming from the detection apparatus.
摘要翻译：一种检测靶部分的方法，其包括以下步骤：（a）提供对靶特异性的抗体，（b）用靶标的标记形式使抗体的结合位点饱和，（c）使包含靶标的液体流过饱和抗体，从而（d）使靶标置换标记的抗原，（e）用标记的检测器检测置换的标记的抗原。此外，本发明通过流动免疫传感器实现，该流动免疫传感器包括：流过免疫传感器的液体，用于使样品移动通过免疫传感器; 用于将样品引入液体流的接收装置; 用于使液体流移动通过免疫传感器的流量控制装置; 连接到样品接收装置的交换器，其中样品与靶标的标记抗原接触，并且由此允许存在的任何靶标置换标记抗原; 连接到交换器的检测装置，用于检测被释放的任何标记的抗原; 以及与检测装置连接的处置装置，用于处置或收集来自检测装置的废物。

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