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1. 发明申请

US20080318288A1 Enzymatic Reduction for Producing Optically Active Alcohols 有权
标题翻译：用于生产光活性醇的酶减量
公开(公告)号：US20080318288A1
公开(公告)日：2008-12-25
申请号：US12158390
申请日：2006-12-13
申请人： Rainer Stürmer , Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Maria Kesseler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
IPC分类号： C12P17/00 , C12N9/14
CPC分类号： C07D333/16 , C07D333/20 , C12P7/02 , C12P11/00 , C12P41/002
摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.
摘要翻译：一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

2. 发明授权

US07888080B2 Enzymatic reduction for producing optically active alcohols 有权
标题翻译：用于制备光学活性醇的酶还原
公开(公告)号：US07888080B2
公开(公告)日：2011-02-15
申请号：US12158390
申请日：2006-12-13
申请人： Rainer Stürmer , Maria Keβeler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Maria Keβeler , Bernhard Hauer , Thomas Friedrich , Michael Breuer , Hartwig Schröder
IPC分类号： C12P17/00 , C12N9/14
CPC分类号： C07D333/16 , C07D333/20 , C12P7/02 , C12P11/00 , C12P41/002
摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.
摘要翻译：一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

3. 发明授权

US08012725B2 Method for the production of optically-active alcohols 有权
标题翻译：光学活性醇的制造方法
公开(公告)号：US08012725B2
公开(公告)日：2011-09-06
申请号：US11667721
申请日：2005-11-15
申请人： Rainer Stürmer , Bernhard Hauer , Dejana Drew , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Bernhard Hauer , Dejana Drew , Michael Breuer , Hartwig Schröder
IPC分类号： C12P7/16 , C12P7/02 , C12P7/04 , C12Q1/26 , C12Q1/32 , C12N1/00 , C12N1/20 , C12N9/02 , C07H21/04
CPC分类号： C12P7/16 , Y02E50/10
摘要： The present invention relates to enzymic processes for preparing S-butan-2-ol; and to enzymes for carrying out said processes; to nucleic acid sequences coding for said enzymes, to expression cassettes, vectors and recombinant hosts containing said nucleic acid sequences.
摘要翻译：本发明涉及制备S-丁-2-醇的酶法; 和用于进行所述方法的酶; 编码所述酶的核酸序列，含有所述核酸序列的表达盒，载体和重组宿主。

4. 发明授权

US07785847B2 Method for producing optically active alcohols from alkanones using a dehydrogenase of azoarcus 有权
标题翻译：使用azoarcus脱氢酶从链烷醇生产光学活性醇的方法
公开(公告)号：US07785847B2
公开(公告)日：2010-08-31
申请号：US11579292
申请日：2005-05-04
申请人： Rainer Stürmer , Maria Keβeler , Bernhard Haur , Thomas Friedrich , Michael Breuer , Hartwig Schröder
发明人： Rainer Stürmer , Maria Keβeler , Bernhard Haur , Thomas Friedrich , Michael Breuer , Hartwig Schröder
IPC分类号： C12P7/22 , C12P7/04 , C12P17/00 , C12N9/04 , C12N9/14 , C12N1/20 , C07H21/04
CPC分类号： C07D333/22 , C07D333/16 , C12P17/00
摘要： The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.
摘要翻译：本发明涉及一种制备式（I）的光学活性链烷醇的方法，其中n为0-5的整数; Cyc表示任选取代的单核或多核，饱和或不饱和的碳环或杂环，并且R 1表示卤素，SH，OH，NO 2，NR 2 R 3或NR 2 R 3 R 4 + X - ，其中R 2，R 3和R 4独立地表示H或低级烷基或低级烷氧基，X-表示抗衡离子。根据本发明，在含有式（II）的烷酮的培养基中温育选自脱氢酶，醛还原酶和羰基还原酶的酶的酶（E），其中n，R 1和R 1如上定义，在存在下的还原当量。将式（II）化合物酶还原成式（I）化合物，并且通过使用酶（E）使牺牲醇与相应的牺牲酮反应，并且至少部分除去牺牲酮来再生反应期间消耗的还原当量从反应介质中分离出如此制备的产物（I）。

5. 发明授权

US06977270B2 Tocotrienolquinone cyclization product with an anti-hypercholesterol effect 有权
标题翻译：生育三烯酚环化产物具有抗高胆固醇的作用
公开(公告)号：US06977270B2
公开(公告)日：2005-12-20
申请号：US10332863
申请日：2001-07-13
申请人： Kai-Uwe Baldenius , Hartwig Schröder , Klaus Krämer , Karin Schein , Rainer Stürmer
发明人： Kai-Uwe Baldenius , Hartwig Schröder , Klaus Krämer , Karin Schein , Rainer Stürmer
IPC分类号： A23L33/105 , A61K31/343 , A61P3/00 , A61P3/06 , A61P3/10 , A61P7/02 , A61P9/00 , A61P9/10 , C07C46/06 , C07C50/28 , C07D307/94 , C07D317/72 , H61K31/343
CPC分类号： C07D307/94 , A23L33/105
摘要： A description is given of the use of at least one compound of the formula I where R1, R2, R3 and R4 independently of one another are H or C1-C6-alkyl; and the use of a compound of the formula I for medical use.
摘要翻译：给出了使用至少一种式I的化合物的用途，其中R 1，R 2，R 3，R 3和R 2 > 4个彼此独立地是H或C 1 -C 6 - 烷基; 以及使用式I化合物用于医疗用途。

6. 发明授权

US06436681B1 Method for producing biotin 失效
标题翻译：生物素生产方法
公开(公告)号：US06436681B1
公开(公告)日：2002-08-20
申请号：US09462645
申请日：2000-01-11
申请人： Hartwig Schröder , Bernhard Hauer
发明人： Hartwig Schröder , Bernhard Hauer
IPC分类号： C12P1718
CPC分类号： C12N9/13 , C12N15/52 , C12P17/186
摘要： A gene construct comprising a biotin gene having the sequence SEQ ID No. 1 or SEQ ID No. 3, organisms which comprise this gene construct, the use of these sequences or of the gene construct for preparing biotin, and a process for preparing biotin are described.
摘要翻译：包含具有序列SEQ ID No.1或SEQ ID No.3的生物素基因的基因构建体，包含该基因构建体的生物体，这些序列或基因构建体用于制备生物素的用途以及制备生物素的方法是描述。

7. 发明授权

US08389251B2 Method for producing methionine using a microorganism with reduced expression activity of a negative regulator of methionine biosynthesis 有权
标题翻译：使用具有降低甲硫氨酸生物合成负调节子的表达活性的微生物生产甲硫氨酸的方法
公开(公告)号：US08389251B2
公开(公告)日：2013-03-05
申请号：US13018119
申请日：2011-01-31
申请人： Markus Pompejus , Burkhard Kröger , Hartwig Schröder , Oskar Zelder , Gregor Haberhauer , Stefan Haefner , Corrinna Klopprogge
发明人： Markus Pompejus , Burkhard Kröger , Hartwig Schröder , Oskar Zelder , Gregor Haberhauer , Stefan Haefner , Corrinna Klopprogge
IPC分类号： C12P13/12 , C12N15/74
CPC分类号： C07K14/34
摘要： Isolated nucleic acid molecules, designated MR nucleic acid molecules, which encode novel MR proteins from Corynebacterium glutamicum are described which are involved in biosynthesis of a fine chemical, e.g., methionine biosynthesis. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MR nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides methods of producing methionine from microorganisms, e.g., C. glutamicum, which involve culturing recombinant microorganisms which overexpress or underexpress at least one MR molecule of the invention under conditions such that methionine is produced. Also featured are methods of producing a fine chemical, e.g., methionine, which involve culturing recombinant microorganisms having selected MR genes deleted or mutated under conditions such that the fine chemical, e.g., methionine, is produced.
摘要翻译：描述了编码来自谷氨酸棒杆菌的新型MR蛋白质的分离的核酸分子，称为MR核酸分子，其涉及精细化学品的生物合成，例如甲硫氨酸生物合成。本发明还提供反义核酸分子，含有MR核酸分子的重组表达载体和已经引入表达载体的宿主细胞。本发明还提供了从微生物例如谷氨酸棒杆菌制备甲硫氨酸的方法，其涉及在产生甲硫氨酸的条件下培养过表达或低表达本发明的至少一种MR分子的重组微生物。还特征在于生产精细化学品的方法，例如甲硫氨酸，其涉及在产生精细化学物质例如甲硫氨酸的条件下培养具有选择的MR基因缺失或突变的重组微生物。

8. 发明申请

US20110008851A1 Carboxylic Acid Producing Member of the Pasteurellaceae 有权
标题翻译：巴氏杆菌科的羧酸生产成员
公开(公告)号：US20110008851A1
公开(公告)日：2011-01-13
申请号：US12673714
申请日：2008-08-14
申请人： Edzard Scholten , Dirk Dägele , Stephan Haefner , Hartwig Schröder
发明人： Edzard Scholten , Dirk Dägele , Stephan Haefner , Hartwig Schröder
IPC分类号： C12P17/10 , C12P17/04 , C12P7/46 , C12P7/18 , C12N1/20 , C12N1/21
CPC分类号： C12P7/46 , C12N1/32 , C12R1/01
摘要： The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism.
摘要翻译：本发明涉及一种称为DD1的新型细菌菌株，其具有生产有机酸特别是琥珀酸（SA）的能力，其最初是从牛瘤胃中分离出来的，能够利用甘油作为碳源; 以及由此导致的变异菌株保留所述能力; 以及通过利用所述微生物生产有机酸，特别是琥珀酸的方法。

9. 发明申请

US20100041107A1 METHOD OF REDUCING GENE EXPRESSION USING MODIFIED CODON USAGE 审中-公开
标题翻译：使用改性CODON使用减少基因表达的方法
公开(公告)号：US20100041107A1
公开(公告)日：2010-02-18
申请号：US12446767
申请日：2007-10-18
申请人： Andrea Herold , Corinna Klopprogge , Hartwig Schröder , Osker Zelder , Weol Kyu Jeong
发明人： Andrea Herold , Corinna Klopprogge , Hartwig Schröder , Osker Zelder , Weol Kyu Jeong
IPC分类号： C12P13/12 , C12P1/00 , C12P13/04 , C12P13/08 , C12P7/64 , C12N5/10 , C12N5/04 , C12N1/21 , C12N15/00 , C12N15/11
CPC分类号： C12N15/77
摘要： The present invention is directed to a method of reducing the amount of at least one polypeptide in a host cell by expressing a nucleotide sequence encoding for the polypeptide in the host cell wherein the nucleotide sequence uses codons that are rarely used according to the codon usage of the host organism. Furthermore, the present invention relates to nucleotide sequences encoding for a polypeptide with a codon usage that has been adjusted to use codons that are only rarely used according to the codon usage of the host organism. The present invention further relates to the use of such sequences and methods for producing fine chemicals such as amino acids, sugars, lipids, oils, carbohydrates, vitamins, cofactors etc.
摘要翻译：本发明涉及通过在宿主细胞中表达编码多肽的核苷酸序列来减少宿主细胞中至少一种多肽的量的方法，其中所述核苷酸序列使用根据密码子使用的密码子使用的密码子宿主生物体。此外，本发明涉及编码具有密码子使用的多肽的核苷酸序列，其已被调整为使用根据宿主生物的密码子使用很少使用的密码子。本发明还涉及这样的序列和方法用于生产精细化学品如氨基酸，糖，脂质，油，碳水化合物，维生素，辅因子等的用途。

10. 发明申请

US20100009416A1 Process for the Preparation of L-Methionine 审中-公开
标题翻译： L-甲硫氨酸的制备方法
公开(公告)号：US20100009416A1
公开(公告)日：2010-01-14
申请号：US12301279
申请日：2007-05-24
申请人： Oskar Zelder , Andrea Herold , Corinna Klopprogge , Hartwig Schröder , Elmar Heinzle , Christoph Wittmann , Jens Kroemer , Janice Pero , Rogers Yocum , Thomas Patterson , Mark Williams , Theron Hermann
发明人： Oskar Zelder , Andrea Herold , Corinna Klopprogge , Hartwig Schröder , Elmar Heinzle , Christoph Wittmann , Jens Kroemer , Janice Pero , Rogers Yocum , Thomas Patterson , Mark Williams , Theron Hermann
IPC分类号： C12P13/12 , C12N1/21
CPC分类号： C12P13/12
摘要： The present invention relates to microorganisms and processes for the efficient preparation of L-methionine. In particular, the present invention relates to processes in which the amount of serine available for the metabolism of the microorganism is increased.
摘要翻译：本发明涉及用于L-甲硫氨酸有效制备的微生物和方法。特别地，本发明涉及其中可用于微生物代谢的丝氨酸量增加的方法。

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