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1. 发明申请

US20100041107A1 METHOD OF REDUCING GENE EXPRESSION USING MODIFIED CODON USAGE 审中-公开
标题翻译：使用改性CODON使用减少基因表达的方法
公开(公告)号：US20100041107A1
公开(公告)日：2010-02-18
申请号：US12446767
申请日：2007-10-18
申请人： Andrea Herold , Corinna Klopprogge , Hartwig Schröder , Osker Zelder , Weol Kyu Jeong
发明人： Andrea Herold , Corinna Klopprogge , Hartwig Schröder , Osker Zelder , Weol Kyu Jeong
IPC分类号： C12P13/12 , C12P1/00 , C12P13/04 , C12P13/08 , C12P7/64 , C12N5/10 , C12N5/04 , C12N1/21 , C12N15/00 , C12N15/11
CPC分类号： C12N15/77
摘要： The present invention is directed to a method of reducing the amount of at least one polypeptide in a host cell by expressing a nucleotide sequence encoding for the polypeptide in the host cell wherein the nucleotide sequence uses codons that are rarely used according to the codon usage of the host organism. Furthermore, the present invention relates to nucleotide sequences encoding for a polypeptide with a codon usage that has been adjusted to use codons that are only rarely used according to the codon usage of the host organism. The present invention further relates to the use of such sequences and methods for producing fine chemicals such as amino acids, sugars, lipids, oils, carbohydrates, vitamins, cofactors etc.
摘要翻译：本发明涉及通过在宿主细胞中表达编码多肽的核苷酸序列来减少宿主细胞中至少一种多肽的量的方法，其中所述核苷酸序列使用根据密码子使用的密码子使用的密码子宿主生物体。此外，本发明涉及编码具有密码子使用的多肽的核苷酸序列，其已被调整为使用根据宿主生物的密码子使用很少使用的密码子。本发明还涉及这样的序列和方法用于生产精细化学品如氨基酸，糖，脂质，油，碳水化合物，维生素，辅因子等的用途。

2. 发明授权

US08771998B2 Process for the production of gamma-aminobutyric acid 失效
标题翻译：生产γ-氨基丁酸的方法
公开(公告)号：US08771998B2
公开(公告)日：2014-07-08
申请号：US12918241
申请日：2009-02-20
申请人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
发明人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
IPC分类号： C12P13/00 , C12N9/88 , C12N1/20 , C12N15/00 , C12P19/34 , C08G69/08 , C07H21/04 , C07K1/00
CPC分类号： C12P13/005 , C12N9/88 , C12P13/02
摘要： The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.
摘要翻译：本发明涉及通过培养表达具有谷氨酸脱羧酶活性的酶的重组微生物来发酵γ-氨基丁酸（GABA）的新方法。本发明还涉及适用于制备这种宿主的相应的重组宿主，重组载体，表达盒和核酸以及使用GABA制备聚酰胺作为获得的发酵生产的方法。

3. 发明授权

US08741623B2 Process for the production of cadaverine 有权
标题翻译：生产尸体素的方法
公开(公告)号：US08741623B2
公开(公告)日：2014-06-03
申请号：US12295348
申请日：2007-03-23
申请人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
发明人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
IPC分类号： C12N1/20 , C12N15/74 , C12N15/77 , A01N63/00 , C07H21/04
CPC分类号： C12P13/02 , C12N9/88 , C12P13/001
摘要： Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.
摘要翻译：通过构建具有失调的赖氨酸脱羧酶基因的重组微生物和选自由天冬氨酸激酶，天冬氨酸二甲醛脱氢酶，二氢吡啶二羧酸合成酶，二氢吡啶二羧酸还原酶，四氢吡啶二羧酸盐琥珀酰基酶，琥珀酰 - 氨基酮基庚酸酯转氨酶，琥珀酰 - 二氨基 - 庚二酸二琥珀酰酶，二氨基庚二酸差向异构酶，二氨基庚二酸脱氢酶，精氨酰-tRNA合成酶，二氨基庚二酸脱羧酶，丙酮酸羧化酶，磷酸烯醇丙酮酸羧化酶，葡萄糖-6-磷酸脱氢酶，转酮酶，转醛醇酶，6-磷酸葡萄糖醛酸酶，果糖1， 6-二磷酸酶，高丝氨酸脱氢酶，磷酸烯醇丙酮酸羧激酶，琥珀酰辅酶A合成酶，甲基丙二酰-CoA变位酶，条件是如果天冬氨酸激酶被释放为基因（i）至少第二个基因（i），除了天冬氨酸激酶外必须解除调节，并培养所述微生物。

4. 发明申请

US20110207183A1 Production Process for Fine Chemicals Using Microorganisms with Reduced Isocitrate Dehydrogenase Activity 审中-公开
标题翻译：使用具有降低的异柠檬酸脱氢酶活性的微生物的精细化学品的生产方法
公开(公告)号：US20110207183A1
公开(公告)日：2011-08-25
申请号：US12990240
申请日：2009-04-28
申请人： Andrea Herold , Hartwig Schröder , Weol Kyu Jeong , Corinna Klopprogge , Oskar Zelder , Stefan Haefner , Ulrike Richter , Judith Becker , Christoph Wittmann
发明人： Andrea Herold , Hartwig Schröder , Weol Kyu Jeong , Corinna Klopprogge , Oskar Zelder , Stefan Haefner , Ulrike Richter , Judith Becker , Christoph Wittmann
IPC分类号： C12P13/20 , C12P1/00 , C12P1/04 , C12P13/04 , C12P13/00 , C12P13/08 , C12N1/00 , C12N1/21 , C12P13/02 , C12P17/12 , C12P17/10 , C12P13/12 , C12P7/40
CPC分类号： C12P13/001 , C12N9/0006 , C12P13/04 , C12P13/08 , C12P17/12 , C12Y101/01041
摘要： The present invention is directed to a method utilizing a microorganism with reduced isocitrate dehydrogenase activity for the production of fine chemicals. Said fine chemicals may be amino acids, monomers for polymer synthesis, sugars, lipids, oils, fatty acids or vitamins and are preferably amino acids of the aspartate family, especially methionine or lysine, or derivatives of said amino acids, especially cadaverine. Furthermore, the present invention relates to a recombinant microorganism having a reduced isocitrate dehydrogenase activity in comparison to the initial microorganism and the use of such microorganisms in producing fine chemicals such as aspartate family amino acids and their derivatives.
摘要翻译：本发明涉及利用具有降低的异柠檬酸脱氢酶活性的微生物来生产精细化学品的方法。所述精细化学品可以是氨基酸，用于聚合物合成的单体，糖，脂质，油，脂肪酸或维生素，并且优选天冬氨酸家族的氨基酸，特别是甲硫氨酸或赖氨酸，或所述氨基酸的衍生物，特别是尸胺。此外，本发明涉及与初始微生物相比具有降低的异柠檬酸脱氢酶活性的重组微生物，以及这些微生物在生产精细化学品如天冬氨酸家族氨基酸及其衍生物中的用途。

5. 发明申请

US20090246838A1 Process For The Production Of Cadaverine 有权
标题翻译：尸曹的生产过程
公开(公告)号：US20090246838A1
公开(公告)日：2009-10-01
申请号：US12295348
申请日：2007-03-23
申请人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
发明人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
IPC分类号： C12P13/00 , C12N1/00 , C12N1/21
CPC分类号： C12P13/02 , C12N9/88 , C12P13/001
摘要： Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.
摘要翻译：通过构建具有失调的赖氨酸脱羧酶基因的重组微生物和选自由天冬氨酸激酶，天冬氨酸二甲醛脱氢酶，二氢吡啶二羧酸合成酶，二氢吡啶二羧酸还原酶，四氢吡啶二羧酸盐琥珀酰基酶，琥珀酰 - 氨基酮基庚酸酯转氨酶，琥珀酰 - 二氨基 - 庚二酸二琥珀酰酶，二氨基庚二酸差向异构酶，二氨基庚二酸脱氢酶，精氨酰-tRNA合成酶，二氨基庚二酸脱羧酶，丙酮酸羧化酶，磷酸烯醇丙酮酸羧化酶，葡萄糖-6-磷酸脱氢酶，转酮酶，转醛醇酶，6-磷酸葡萄糖醛酸酶，果糖1， 6-二磷酸酶，高丝氨酸脱氢酶，磷酸烯醇丙酮酸羧激酶，琥珀酰辅酶A合成酶，甲基丙二酰-CoA变位酶，条件是如果天冬氨酸激酶被释放为基因（i）至少第二个基因（i），除了天冬氨酸激酶外必须解除调节，并培养所述微生物。

6. 发明申请

US20110003963A1 METHOD FOR THE PRODUCTION OF DIPICOLINATE 审中-公开
标题翻译：二氯化碳生产方法
公开(公告)号：US20110003963A1
公开(公告)日：2011-01-06
申请号：US12865895
申请日：2009-02-04
申请人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
发明人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
IPC分类号： C08G69/08 , C12P17/12 , C07H21/04 , C12N15/63 , C12N1/21
CPC分类号： C12P17/12 , C12N9/001
摘要： The present invention relates to a novel method for the fermentative production of dipicolinate by cultivating a recombinant microorganism expressing an enzyme having dipicolinate synthetase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as a method of preparing polyester or polyamide copolymers making use of dipicolinate as obtained by fermentative production.
摘要翻译：本发明涉及通过培养表达具有吡啶二羧酸合成酶活性的酶的重组微生物来发酵吡啶硫酸盐的新方法。本发明还涉及适用于制备这种宿主的相应的重组宿主，重组载体，表达盒和核酸以及使用通过发酵生产得到的吡啶二酸盐制备聚酯或聚酰胺共聚物的方法。

7. 发明申请

US20090325244A1 METHOD OF INCREASING GENE EXPRESSION USING MODIFIED CODON USAGE 审中-公开
标题翻译：使用改性CODON使用增加基因表达的方法
公开(公告)号：US20090325244A1
公开(公告)日：2009-12-31
申请号：US12446809
申请日：2007-10-18
申请人： Andrea Herold , Corinna Klopprogge , Hartwig Schröder , Oskar Zelder , Weol Kyu Jeong
发明人： Andrea Herold , Corinna Klopprogge , Hartwig Schröder , Oskar Zelder , Weol Kyu Jeong
IPC分类号： C12P13/12 , C12N15/87 , C12N15/82 , C12N15/11 , C12N15/00 , C12N1/21 , C12P13/04 , C12P13/08 , C12P7/64
CPC分类号： C12N15/67 , C12N15/77
摘要： The present invention relates to a method of increasing the amount of at least one polypeptide in the host cell by expressing a modified nucleotide sequence encoding for a polypeptide in a host cell with said modified nucleotide sequence being derived from a different non-modified nucleotide sequence encoding for a polypeptide of identical amino acid sequence such that the codon usage of the modified nucleotide sequence is adjusted to the codon usage of abundant proteins in the host cell.
摘要翻译：本发明涉及一种通过在宿主细胞中表达编码多肽的修饰的核苷酸序列来增加宿主细胞中至少一种多肽的量的方法，所述修饰的核苷酸序列来自编码的不同的未修饰的核苷酸序列对于相同氨基酸序列的多肽，使得修饰的核苷酸序列的密码子使用被调节为宿主细胞中丰富蛋白质的密码子使用。

8. 发明申请

US20140256005A1 PROCESS FOR THE PRODUCTION OF GAMMA-AMINOBUTYRIC ACID 审中-公开
标题翻译：制备游离氨基酸的方法
公开(公告)号：US20140256005A1
公开(公告)日：2014-09-11
申请号：US14289731
申请日：2014-05-29
申请人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
发明人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
IPC分类号： C12P13/00 , C12P13/02 , C12N9/88
CPC分类号： C12P13/005 , C12N9/88 , C12P13/02
摘要： The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.
摘要翻译：本发明涉及通过培养表达具有谷氨酸脱羧酶活性的酶的重组微生物来发酵γ-氨基丁酸（GABA）的新方法。本发明还涉及适用于制备这种宿主的相应的重组宿主，重组载体，表达盒和核酸以及使用GABA制备聚酰胺作为获得的发酵生产的方法。

9. 发明申请

US20100324258A1 Process for the Production of Gamma-Aminobutyric Acid 失效
标题翻译： γ-氨基丁酸生产工艺
公开(公告)号：US20100324258A1
公开(公告)日：2010-12-23
申请号：US12918241
申请日：2009-02-20
申请人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
发明人： Oskar Zelder , Weol Kyu Jeong , Corinna Klopprogge , Andrea Herold , Hartwig Schröder
IPC分类号： C08G69/08 , C12P13/00 , C12N9/88 , C12N15/60 , C12N15/63 , C12N1/21
CPC分类号： C12P13/005 , C12N9/88 , C12P13/02
摘要： The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant micro-organism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.
摘要翻译：本发明涉及通过培养表达具有谷氨酸脱羧酶活性的酶的重组微生物来发酵γ-氨基丁酸（GABA）的新方法。本发明还涉及适用于制备这种宿主的相应的重组宿主，重组载体，表达盒和核酸以及使用GABA制备聚酰胺作为获得的发酵生产的方法。

10. 发明申请

US20120295317A1 Processes and Recombinant Microorganisms for the Production of Cadaverine 审中-公开
标题翻译：用于生产尸胺的方法和重组微生物
公开(公告)号：US20120295317A1
公开(公告)日：2012-11-22
申请号：US13516034
申请日：2010-12-15
申请人： Hartwig Schröder , Weol Kyu Jeong , Annegret Serwe , Oskar Zelder
发明人： Hartwig Schröder , Weol Kyu Jeong , Annegret Serwe , Oskar Zelder
IPC分类号： C12P13/00 , C07C209/86 , C12N1/21
CPC分类号： C12N9/88 , C12P13/001
摘要： The present invention relates to the use of recombinant microorganisms comprising DNA molecules in a deregulated form which improve the production of cadaverine, as well as to recombinant DNA molecules and polypeptides used to produce the microorganism, said microorganism comprising an intracellular lysine decarboxylase activity and an enhanced lysine import activity or comprising an intracellular and an extracellular lysine decarboxylase activity or comprising an intracellular and extracellular lysine decarboxylase activity and an enhanced lysine import activity. The present invention also relates to a processes for the production of cadaverine using recombinant microorganisms.
摘要翻译：本发明涉及包含DNA分子的重组微生物在去调节形式中的用途，其改善了尸胺的产生，以及用于产生微生物的重组DNA分子和多肽，所述微生物包含细胞内赖氨酸脱羧酶活性和增强的赖氨酸进入活性或包含细胞内和细胞外赖氨酸脱羧酶活性或包含细胞内和细胞外赖氨酸脱羧酶活性和增强的赖氨酸进口活性。本发明还涉及使用重组微生物生产尸胺的方法。

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