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2. 发明申请

WO0216935A9 SCREENING METHOD FOR DISCOVERING AUXILIARY AGENTS PROMOTING ENDOCYTOSIS 审中-公开
标题翻译：检查方法用于确定促销的内吞作用的佐剂
公开(公告)号：WO0216935A9
公开(公告)日：2003-09-04
申请号：PCT/EP0107509
申请日：2001-06-30
申请人： RINA NETZWERK RNA TECHNOLOGIEN , LANG CHRISTINE , GESSNER REINHARD , NEUKAMM BIRGIT , PRINZ BIANKA , NEVOIGT ELKE
发明人： LANG CHRISTINE , GESSNER REINHARD , NEUKAMM BIRGIT , PRINZ BIANKA , NEVOIGT ELKE
IPC分类号： C12Q1/02 , G01N33/50
CPC分类号： G01N33/502 , C12Q1/025 , G01N33/5008
摘要： The invention relates to a screening method for discovering auxiliary agents promoting the endocytosis of low molecular and/or macro-molecular compounds, especially nucleic acids, in cells. The inventive method comprises the following steps: a) a yeast strain is selected and yeast cells are cultivated therefrom; b) the yeast cells are incubated with a potential auxiliary agent promoting the endocytosis of low molecular and/or macro-molecular compounds, especially nucleic acids, in cells in addition to a nucleic acid which is replicable and/or replicable in the nucleus and/or in the cytosol of the yeast cells or a low molecular and/or macro-molecular compound, c) the transformation rates or the transport rates into the cytosol or the nucleus are determined and analysis occurs if the transformation rates or transport rates increase in comparison with the transformation rates and transport rates in a reference test in which the auxiliary substance is not used, d) the auxiliary substance is selected or rejected according to the result of the value obtained for the increase in the transformation rates or transport rates or transport rates in step c). The invention also relates to a screening method of the above-mentioned variety, wherein vertebrate cells are used, in addition to the use of auxiliary agents obtained according to said method in order to produce pharmaceutical substances.
摘要翻译：本发明教导了一种筛选方法，用于发现低和/或大分子化合物的细胞内吞作用，特别是核酸，细胞，包括以下方法步骤：a）的酵母菌株被选择和酵母细胞中培养由此，b）将酵母细胞用一个潜在的孵育低和/或大分子化合物的细胞内吞作用，特别是核酸，细胞，赋形剂和具有可复制的和/或在核心和/或酵母细胞中复制的核酸或低和/或大分子化合物c的胞质溶胶）确定转化率或运输速率进入细胞质或细胞核，和转化率或运输速率的增加，相对于转化率或在参考测试输送速率，而不使用辅助物质，检查，d）中的赋形剂是NA selektriert或丢弃 CH小于本所得到的转化率的增加或在步骤c运输速率），使用脊椎动物细胞以及与这样的用于生产药物组合物的方法获得的佐剂使用的筛选方法对应的方式的值。

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