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1. 发明专利

JP2005304504A Composition and method for purifying nucleic acid 有权
标题翻译：用于纯化核酸的组合物和方法
公开(公告)号：JP2005304504A
公开(公告)日：2005-11-04
申请号：JP2005146671
申请日：2005-05-19
申请人： Promega Corp , プロメガ・コーポレイシヨン
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1006 , Y10T428/2996
摘要： PROBLEM TO BE SOLVED: To provide compositions and methods for isolating nucleic acids with lengths greater than 50 bases in a state suitable for following treatments or analysis from other substances binding to nucleic acids in vivo or in vitro. SOLUTION: Disclosed are mixtures of silica materials, silica gel and glass particles, particularly glass microfibers; such mixtures of chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. An aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid binds to the silica materials. After materials other than the nucleic acid are washed from the silica materials, the nucleic acid can be obtained by elution from the silica materials. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供用于分离长度大于50个碱基的核酸的组合物和方法，所述核酸适合于在体内或体外与核酸结合的其它物质的后续处理或分析。解决方案：公开了二氧化硅材料，硅胶和玻璃颗粒，特别是玻璃微纤维的混合物; 离液盐如氯化胍或硫氰酸胍的混合物; 和这种混合物在离液盐水溶液中的悬浮液。将包含核酸的水溶液与离液盐的水溶液混合，并将所得溶液与二氧化硅材料的混合物接触，由此核酸与二氧化硅材料结合。在从二氧化硅材料洗涤核酸以外的材料之后，可以从二氧化硅材料中洗脱来获得核酸。版权所有（C）2006，JPO＆NCIPI

2. 发明授权

US5658548C1 Nucleic acid purification on silica geland glass mixtures 失效
标题翻译：硅胶和玻璃混合物上的核酸纯化
公开(公告)号：US5658548C1
公开(公告)日：2001-07-24
申请号：US47684995
申请日：1995-06-07
申请人： PROMEGA CORP
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C03C1/00 , C01B33/12 , C01B33/14 , C03C12/00
CPC分类号： C12Q1/6806 , C12N15/1006 , Y10T428/2996

3. 发明专利

AT256735T 未知
公开(公告)号：AT256735T
公开(公告)日：2004-01-15
申请号：AT94931272
申请日：1994-08-30
申请人： PROMEGA CORP
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C12Q1/6806 , C07H1/06 , C08K3/36 , C08K5/31
摘要： The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

4. 发明专利

CA2170604C NUCLEIC ACID PURIFICATION COMPOSITIONS AND METHODS 未知
公开(公告)号：CA2170604C
公开(公告)日：2007-03-13
申请号：CA2170604
申请日：1994-08-30
申请人： PROMEGA CORP
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C07H21/00 , C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C12Q1/6806
摘要： The present invention provides compositions and methods for isolating nuclei c acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid ca n be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

5. 发明专利

AU8010094A Nucleic acid purification compositions and methods 未知
公开(公告)号：AU8010094A
公开(公告)日：1995-03-22
申请号：AU8010094
申请日：1994-08-30
申请人： PROMEGA CORP
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C12Q1/6806 , C07H1/06 , C07H21/00 , C08K5/31
摘要： The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

6. 发明公开

EP0723549A4 NUCLEIC ACID PURIFICATION COMPOSITIONS AND METHODS 失效
标题翻译：组合物和方法用于纯化核酸
公开(公告)号：EP0723549A4
公开(公告)日：1998-05-06
申请号：EP94931272
申请日：1994-08-30
申请人： PROMEGA CORP
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C07H1/06 , C07H21/00 , C08K5/31
CPC分类号： C12Q1/6806 , C12N15/1006 , Y10T428/2996
摘要： The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

7. 发明专利

DE69433425T2 未知
公开(公告)号：DE69433425T2
公开(公告)日：2004-10-07
申请号：DE69433425
申请日：1994-08-30
申请人： PROMEGA CORP
发明人： PADHYE V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C12Q1/6806 , C07H1/06 , C08K3/36 , C08K5/31
摘要： The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

8. 发明专利

DE69433425D1 未知
公开(公告)号：DE69433425D1
公开(公告)日：2004-01-29
申请号：DE69433425
申请日：1994-08-30
申请人： PROMEGA CORP
发明人： PADHYE V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C12Q1/6806 , C07H1/06 , C08K3/36 , C08K5/31
摘要： The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

9. 发明专利

AU689815B2 Nucleic acid purification compositions and methods 未知
公开(公告)号：AU689815B2
公开(公告)日：1998-04-09
申请号：AU8010094
申请日：1994-08-30
申请人： PROMEGA CORP
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C12Q1/6806 , C07H1/06 , C07H21/00 , C08K5/31
摘要： The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

10. 发明专利

CA2170604A1 NUCLEIC ACID PURIFICATION COMPOSITIONS AND METHODS 未知
公开(公告)号：CA2170604A1
公开(公告)日：1995-03-09
申请号：CA2170604
申请日：1994-08-30
申请人： PROMEGA CORP
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68 , C12Q1/6806 , C07H21/00 , G01N30/48
摘要： The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

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