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1. 发明专利

JP2010104377A Thermostable luciferase and method of production 有权
标题翻译：热稳定脱脂酶及其生产方法
公开(公告)号：JP2010104377A
公开(公告)日：2010-05-13
申请号：JP2010006999
申请日：2010-01-15
申请人： Promega Corp , プロメガ・コーポレイシヨン
发明人： WOOD KEITH V , HALL MARY P
IPC分类号： C12N15/09 , C07K19/00 , C12N1/21 , C12N9/02 , C12N11/00 , C12N15/10 , C12N15/53 , C12P21/02 , C12Q1/02 , C12Q1/66 , C12Q1/68
CPC分类号： C12N9/0069 , C12N15/10
摘要： PROBLEM TO BE SOLVED: To provide mutant luciferase enzymes with greatly enhanced thermostability in comparison with natural luciferases or mutant luciferases. SOLUTION: A modified luciferase conjugate is prepared by using a beetle luciferase gene, applying it to various means for inducing random mutation, particularly to gene synthesis using a polymerase easily happening to be in errors, preparing a modified luciferase gene library, expressing the same with E. coli, and combining selected mutations. The method includes recursive mutagenesis in which a novel library is prepared from modified luciferases through random mutation induction and the process is repeatedly selected. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：与天然荧光素酶或突变荧光素酶相比，提供具有极大增强的热稳定性的突变荧光素酶。解决方案：通过使用甲虫荧光素酶基因，将其应用于诱导随机突变的各种手段，特别是使用容易发生错误的聚合酶的基因合成，制备修饰的荧光素酶基因文库，表达与大肠杆菌相同，并结合选定的突变。该方法包括递归诱变，其中通过随机突变诱导从修饰的萤光素酶制备新文库，并重复选择该过程。版权所有（C）2010，JPO＆INPIT

2. 发明专利

JP2005304504A Composition and method for purifying nucleic acid 有权
标题翻译：用于纯化核酸的组合物和方法
公开(公告)号：JP2005304504A
公开(公告)日：2005-11-04
申请号：JP2005146671
申请日：2005-05-19
申请人： Promega Corp , プロメガ・コーポレイシヨン
发明人： PADHYE VIKAS V , YORK CHUCK , BURKIEWICZ ADAM
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1006 , Y10T428/2996
摘要： PROBLEM TO BE SOLVED: To provide compositions and methods for isolating nucleic acids with lengths greater than 50 bases in a state suitable for following treatments or analysis from other substances binding to nucleic acids in vivo or in vitro. SOLUTION: Disclosed are mixtures of silica materials, silica gel and glass particles, particularly glass microfibers; such mixtures of chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. An aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid binds to the silica materials. After materials other than the nucleic acid are washed from the silica materials, the nucleic acid can be obtained by elution from the silica materials. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供用于分离长度大于50个碱基的核酸的组合物和方法，所述核酸适合于在体内或体外与核酸结合的其它物质的后续处理或分析。解决方案：公开了二氧化硅材料，硅胶和玻璃颗粒，特别是玻璃微纤维的混合物; 离液盐如氯化胍或硫氰酸胍的混合物; 和这种混合物在离液盐水溶液中的悬浮液。将包含核酸的水溶液与离液盐的水溶液混合，并将所得溶液与二氧化硅材料的混合物接触，由此核酸与二氧化硅材料结合。在从二氧化硅材料洗涤核酸以外的材料之后，可以从二氧化硅材料中洗脱来获得核酸。版权所有（C）2006，JPO＆NCIPI

3. 发明专利

JP3673175B2 Stabilization of enzymes by the cationic surfactant 有权
公开(公告)号：JP3673175B2
公开(公告)日：2005-07-20
申请号：JP2000556016
申请日：1999-06-23
申请人：プロメガ・コーポレイシヨン
发明人：シュルツ，ジョン，ダブリュ． , フアン，フェン
IPC分类号： C12N9/12 , C12N20060101 , C12N9/96
CPC分类号： C12N9/12 , C12N9/1241 , C12N9/1252 , C12N9/1276 , C12N9/96

4. 发明专利

JP3696238B2 The compositions and methods for nucleic acid purification 失效
公开(公告)号：JP3696238B2
公开(公告)日：2005-09-14
申请号：JP50831595
申请日：1994-08-30
申请人：プロメガ・コーポレイシヨン
发明人：パデイ，ビカス・ブイ , ブルツキイウイツツ，アダム , ヨーク，チヤツク
IPC分类号： C12N15/00 , B01D15/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1006 , Y10T428/2996

5. 发明专利

JP4480622B2 The compositions and methods for nucleic acid purification 有权
公开(公告)号：JP4480622B2
公开(公告)日：2010-06-16
申请号：JP2005146671
申请日：2005-05-19
申请人：プロメガ・コーポレイシヨン
发明人：アダム・ブルツキイウイツツ , チヤツク・ヨーク , ビカス・ブイ・パデイ
IPC分类号： C07H1/06 , C12N15/00 , B01D15/00 , C07H21/00 , C08K3/34 , C08K3/40 , C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1006 , Y10T428/2996
摘要： The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

6. 发明专利

JP4537573B2 How production and heat stable luciferase 有权
公开(公告)号：JP4537573B2
公开(公告)日：2010-09-01
申请号：JP2000511874
申请日：1998-09-18
申请人：プロメガ・コーポレイシヨン
发明人：ウツド，キース・ブイ , ホール，メアリー・ピー
IPC分类号： C12N9/02 , C12N15/09 , C07K19/00 , C12N1/21 , C12N11/02 , C12N11/14 , C12N15/10 , C12N15/53 , C12Q1/26 , C12Q1/68 , C12R1/19
CPC分类号： C12N9/0069 , C12N15/10

7. 发明专利

JP2009039129A Mutant luciferase 有权
标题翻译： MUTANT LUCIFERASE
公开(公告)号：JP2009039129A
公开(公告)日：2009-02-26
申请号：JP2008244507
申请日：2008-09-24
申请人： Promega Corp , プロメガ・コーポレイシヨン
发明人： WOOD KEITH V , GRUBER MONIKA G
IPC分类号： C12N15/09 , C12N9/02 , C12N15/53 , C12P21/02
CPC分类号： C12N9/0069 , C12Y113/12007
摘要： PROBLEM TO BE SOLVED: To provide a non-natural active mutant of a beetle luciferase, and a DNA encoding such a mutant. SOLUTION: This mutant luciferase is obtained from beetles. The mutant luciferase is different from a corresponding wild type luciferase by a fact that it generates a biological luminescence having peak intensity at a wavelength different by at least 1 nm from the peak intensity of the biological luminescence generated from the wild type enzyme. The mutant luciferase and DNA can be utilized for various biological detections. COPYRIGHT: (C)2009,JPO&INPIT

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