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1. 发明授权

US06482306B1 Meso- and microfluidic continuous flow and stopped flow electroösmotic mixer 失效
标题翻译：介质和微流体连续流动和停止流动电渗流混合器
公开(公告)号：US06482306B1
公开(公告)日：2002-11-19
申请号：US09404454
申请日：1999-09-22
申请人： Paul Yager , Mark R. Holl , Andrew Kamholz , Catherine Cabrera , Katerina Macounova
发明人： Paul Yager , Mark R. Holl , Andrew Kamholz , Catherine Cabrera , Katerina Macounova
IPC分类号： B01F1300
CPC分类号： B01F13/0076 , B01F13/0001 , B01F13/0005 , B01F13/0077 , B01L3/5027 , Y10T137/2087 , Y10T137/2191
摘要： An electroösmotic mixing device and a method for mixing one or more fluids for use in meso- or microfluidic device applications. The mixing device provides batch or continuous mixing of one or more fluids in meso- or microfluidic channels. An electric field is generated in the channel in substantial contact with chargeable surfaces therein. No alterations of the geometry of existing flow paths need be made, and the degree of mixing in the device can be controlled by the length of the electrodes, the flow rate past the electrodes, and the voltage applied to those electrodes. The degree of mixing is affected by choice of materials for the chargeable surface (in some cases by the selection of materials or coatings for channel walls) and the ionic strength of the fluids and the type and concentration of ions in the fluids. The ionic strength of fluids to be mixed is sufficiently low to allow electroosmotic flow. The method and device of this invention is preferably applied to fluids to having low ionic strength less than or equal to about 1 mM.
摘要翻译：一种电渗混合装置和用于混合用于中等或微流体装置应用的一种或多种流体的方法。混合装置在中等或微流体通道中提供一种或多种流体的间歇或连续混合。在通道中产生与其中的可充电表面实质接触的电场。不需要改变现有流路的几何形状，并且可以通过电极的长度，通过电极的流速和施加到这些电极的电压来控制装置中的混合程度。混合的程度受到可充电表面的材料选择（在某些情况下通过选择通道壁的材料或涂层）以及流体的离子强度以及流体中离子的类型和浓度的影响。要混合的流体的离子强度足够低以允许电渗流。本发明的方法和装置优选应用于具有小于或等于约1mM的低离子强度的流体。

2. 发明授权

US5972710A Microfabricated diffusion-based chemical sensor 失效
标题翻译：微型基于扩散的化学传感器
公开(公告)号：US5972710A
公开(公告)日：1999-10-26
申请号：US829679
申请日：1997-03-31
申请人： Bernhard H. Weigl , Paul Yager , James P. Brody , Mark R. Holl , Margaret Kenny , David Schutte , Gregory Hixson , M. Diane Zebert , Andrew Kamholz , Caicai Wu , Eric Altendorf
发明人： Bernhard H. Weigl , Paul Yager , James P. Brody , Mark R. Holl , Margaret Kenny , David Schutte , Gregory Hixson , M. Diane Zebert , Andrew Kamholz , Caicai Wu , Eric Altendorf
IPC分类号： G01N37/00 , B01F5/04 , B01F13/00 , B01J19/00 , B01L3/00 , G01N15/06 , G01N15/14 , G01N30/00 , G01N30/38 , G01N30/60 , G01N30/74 , G01N31/20 , G01N35/08 , G01N21/64
CPC分类号： B01F5/0403 , B01F13/0059 , B01F13/0093 , B01J19/0093 , B01L3/502776 , G01N30/0005 , B01J2219/00828 , B01J2219/00831 , B01J2219/00952 , B01L2200/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , G01N15/14 , G01N30/38 , G01N30/6095 , G01N30/74 , G01N35/085 , Y10S366/01 , Y10T436/25375 , Y10T436/2575
摘要： A channel-cell system is provided for detecting the presence and/or measuring the presence of analyte particles in a sample stream comprising: a) a laminar flow channel; b) two inlets in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may comprise an indicator substance which indicates the presence of the analyte particles by a detectable change in property when contacted with the analyte particles, and (2) the sample stream; c) wherein the laminar flow channel has a depth sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to the substantial exclusion of the larger particles in the sample stream to form a detection area; and d) an outlet for conducting the streams out of the laminar flow channel to form a single mixed stream.
摘要翻译：提供了一种通道细胞系统，用于检测样品流中存在和/或测量分析物颗粒的存在，其包括：a）层流通道; b）与层流通道流体连接的两个入口，用于分别导入层流通道（1）中，指示物流可包含指示物质，其指示物质与当与分析物颗粒，和（2）样品流; c）其中层流通道具有足够小的深度以允许流的层流，并且足够的长度允许分析物的颗粒扩散到指示物流中，从而基本排除样品流中的较大颗粒，形成检测区; 和d）用于将物流从层流通道导出以形成单一混合流的出口。

3. 发明授权

US06454945B1 Microfabricated devices and methods 有权
标题翻译：微型装置和方法
公开(公告)号：US06454945B1
公开(公告)日：2002-09-24
申请号：US09703764
申请日：2000-11-01
申请人： Bernhard H. Weigl , Paul Yager , James P. Brody , Mark R. Holl , Fred K. Forster , Eric Altendorf , Paul C. Galambos , Margaret Kenny , David Schutte , Gregory Hixson , Diane Zebert , Andrew Kamholz , Caicai Wu
发明人： Bernhard H. Weigl , Paul Yager , James P. Brody , Mark R. Holl , Fred K. Forster , Eric Altendorf , Paul C. Galambos , Margaret Kenny , David Schutte , Gregory Hixson , Diane Zebert , Andrew Kamholz , Caicai Wu
IPC分类号： B02D1100
CPC分类号： B01D11/0492 , A61M1/14 , B01D11/0496 , B01L3/5027 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2300/0816 , B01L2300/0867 , B01L2400/0406 , B01L2400/0487 , G01N15/1484 , Y10T436/25375
摘要： This invention provides microfabricated systems for extraction of desired particles from a sample stream containing desired and undesired particles. The sample stream is placed in laminar flow contact with an extraction stream under conditions in which inertial effects are negligible. The contact between the two streams is maintained for a sufficient period of time to allow differential transport of the desired particles from the sample stream into the extraction stream. In a preferred embodiment the differential transport mechanism is diffusion. The extraction system of this invention coupled to a microfabricated diffusion-based mixing device and/or sensing means allows picoliter quantities of fluid to be processed or analyzed on devices no larger than silicon wafers. Such diffusion-based mixing or sensing devices are preferably channel cell systems for detecting the presence and/or measuring the quantity of analyte particles in a sample stream.
摘要翻译：本发明提供了用于从含有所需和不需要的颗粒的样品流中提取所需颗粒的微制造系统。在惯性效应可忽略的条件下，将样品流与提取流层流接触。保持两个物流之间的接触足够的时间，以便将期望的颗粒从样品流中差分输送到萃取物流中。在优选实施例中，差分传送机构是扩散。本发明的提取系统耦合到基于微制造的基于扩散的混合装置和/或感测装置允许在不大于硅晶片的装置上处理或分析皮升数量的流体。这种基于扩散的混合或感测装置优选是用于检测样品流中分析物颗粒的存在和/或测量量的通道电池系统。

4. 发明授权

US5716852A Microfabricated diffusion-based chemical sensor 失效
公开(公告)号：US5716852A
公开(公告)日：1998-02-10
申请号：US625808
申请日：1996-03-29
申请人： Paul Yager , Bernhard H. Weigl , James P Brody , Mark R. Holl
发明人： Paul Yager , Bernhard H. Weigl , James P Brody , Mark R. Holl
IPC分类号： G01N37/00 , B01F5/04 , B01F13/00 , B01J19/00 , B01L3/00 , G01N15/06 , G01N15/14 , G01N30/00 , G01N30/38 , G01N30/60 , G01N30/74 , G01N31/20 , G01N35/08 , G01N21/64
CPC分类号： B01F5/0403 , B01F13/0059 , B01F13/0093 , B01J19/0093 , B01L3/502776 , G01N30/0005 , B01J2219/00828 , B01J2219/00831 , B01J2219/00952 , B01L2200/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , G01N15/14 , G01N30/38 , G01N30/6095 , G01N30/74 , G01N35/085 , Y10S366/01 , Y10T436/25375 , Y10T436/2575
摘要： A channel-cell system is provided for detecting the presence and/or measuring the presence of analyte particles in a sample stream comprising: a) a laminar flow channel; b) two inlet means in fluid connection with said laminar flow channel for respectively conducting into said laminar flow channel (1) an indicator stream which may comprise an indicator substance which indicates the presence of said analyte particles by a detectable change in property when contacted with said analyte particles, and (2) said sample stream; c) wherein said laminar flow channel has a depth sufficiently small to allow laminar flow of said streams and a length sufficient to allow particles of said analyte to diffuse into said indicator stream to the substantial exclusion of said larger particles in said sample stream to form a detection area; and d) outlet means for conducting said streams out of said laminar flow channel to form a single mixed stream.

5. 发明授权

US5932100A Microfabricated differential extraction device and method 失效
标题翻译：微型微分提取装置及方法
公开(公告)号：US5932100A
公开(公告)日：1999-08-03
申请号：US663916
申请日：1996-06-14
申请人： Paul Yager , James P. Brody , Mark R. Holl , Fred K. Forster , Paul C. Galambos
发明人： Paul Yager , James P. Brody , Mark R. Holl , Fred K. Forster , Paul C. Galambos
IPC分类号： B01D11/00 , B01D11/02 , B01D11/04 , B01D43/00 , B01F13/00 , B01J19/00 , G01N1/02 , G01N1/10 , G01N1/40 , G01N15/06 , G01N30/00 , G01N35/00 , G01N37/00
CPC分类号： B01D11/0492 , B01D11/0496 , B01F13/0059 , B01F13/0093 , B01J19/0093 , G01N15/06 , G01N30/0005 , B01F2005/0031 , B01J2219/00783 , B01J2219/00828 , B01J2219/00889 , B01J2219/00905 , B01J2219/00952 , B01J2219/0097 , B01J2219/00995 , G01N2001/4072 , G01N2035/00237 , Y10S366/01 , Y10T436/255
摘要： This invention provides a microfabricated extraction system and methods for extracting desired particles from a sample stream containing desired and undesired particles. The sample stream is placed in laminar flow contact with an extraction stream under conditions in which inertial effects are negligible. The contact between the two streams is maintained for a sufficient period of time to allow differential transport of the desired particles from the sample stream into the extraction stream. In a preferred embodiment the differential transport mechanism is diffusion. The extraction system of this invention coupled to a microfabricated diffusion-based mixing device and/or sensing device allows picoliter quantities of fluid to be processed or analyzed on devices no larger than silicon wafers.
摘要翻译：本发明提供了一种微加工提取系统和从含有所需和不需要的颗粒的样品流中提取所需颗粒的方法。在惯性效应可忽略的条件下，将样品流与提取流层流接触。保持两个物流之间的接触足够的时间，以便将期望的颗粒从样品流中差分输送到萃取物流中。在优选实施例中，差分传送机构是扩散。本发明的提取系统耦合到基于微制造的基于扩散的混合装置和/或感测装置允许在不大于硅晶片的装置上处理或分析皮升数量的流体。

6. 发明申请

US20120231533A1 DEVICE AND METHOD FOR THE STUDY OF CELL AND TISSUE FUNCTION 审中-公开
标题翻译：用于细胞和组织功能研究的装置和方法
公开(公告)号：US20120231533A1
公开(公告)日：2012-09-13
申请号：US13126700
申请日：2009-10-28
申请人： Mark R. Holl , Deirdre R. Meldrum , A. Cody Youngbull , Haixin Zhu , Jeff Houkal , Yanqing Tian , Shashanka Ashili , Laimonas Kelbauskas , Roger Johnson , Shih-hui Chao , Peter Wiktor , Alex Jen , Lloyd Burgess , Sarah McQuaid , Ai Brunner , Peter Kahn
发明人： Mark R. Holl , Deirdre R. Meldrum , A. Cody Youngbull , Haixin Zhu , Jeff Houkal , Yanqing Tian , Shashanka Ashili , Laimonas Kelbauskas , Roger Johnson , Shih-hui Chao , Peter Wiktor , Alex Jen , Lloyd Burgess , Sarah McQuaid , Ai Brunner , Peter Kahn
IPC分类号： C12M1/34
CPC分类号： C12M23/16 , C12M23/12 , C12M23/22 , C12M23/38 , C12M41/46
摘要： A chamber device for analyzing living cell(s). The chamber device includes a base and a lid that when reversibly pressed closed create a chamber. The base is configured with an optically transparent well to contain at least one cell. The lid has a breadth greater than the base and is configured to contain at least one sensor. The lid is further configured with a lip that when pressed between the lid and the base creates an impermeable seal. The base and the lid are configured so that, when closed and in use, the sensor remains spatially apart from the at least one cell.
摘要翻译：用于分析活细胞的室装置。腔室装置包括底座和盖子，当可逆地按压时，盖子产生一个腔室。基底配置有光学透明的孔以容纳至少一个细胞。盖子的宽度大于基座，并被配置成包含至少一个传感器。盖子进一步配置有唇部，当在盖子和基座之间被按压时，唇部产生不可渗透的密封。底座和盖被构造成使得当关闭并且在使用中时，传感器在空间上保持与至少一个单元隔开。

7. 发明授权

US06582963B1 Simultaneous analyte determination and reference balancing in reference T-sensor devices 有权
标题翻译：参考T传感器设备中的同时分析物测定和参考平衡
公开(公告)号：US06582963B1
公开(公告)日：2003-06-24
申请号：US09702645
申请日：2000-10-31
申请人： Bernhard H. Weigl , Mark R. Holl , Diane Zebert , Margaret Kenny , Caicai Wu
发明人： Bernhard H. Weigl , Mark R. Holl , Diane Zebert , Margaret Kenny , Caicai Wu
IPC分类号： G01N3508
CPC分类号： B01F13/0093 , B01F5/0403 , B01F13/0059 , B01J19/0093 , B01L3/502776 , B01L2200/0636 , B01L2200/148 , B01L2300/0816 , B01L2400/0406 , B01L2400/0415 , B01L2400/0457 , B01L2400/0487 , G01N15/10 , G01N15/14 , G01N15/1404 , G01N15/1484 , G01N30/0005 , G01N30/38 , G01N30/6095 , G01N30/74 , G01N35/085 , G01N2035/00237 , Y10T436/117497 , Y10T436/118339 , Y10T436/25375 , Y10T436/2575
摘要： A reference T-sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlets in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of analyte particles by a detectable change in property when contacted with the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or an internal standard stream; c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area; and (d) an outlet for conducting the streams out of the laminar flow channel preferably to form a single mixed stream.
摘要翻译：提供了一种参考T传感器系统，用于检测样品流中分析物颗粒的存在和/或测量浓度。该系统包括：a）层流通道; b）与层流通道流体连接的三个或更多个入口，用于分别导入层流通道（1）中，指示物流可包括指示剂物质，其指示当与分析物颗粒，（2）样品流，和（3）参考流，其可以是对流和/或内标流; c）其中层流通道具有足够小的深度和/或宽度以允许流的层流，并且具有足以允许分析物的颗粒扩散到指示物流中以形成检测区域的长度; 和（d）用于将流从层流通道引导出来以形成单一混合流的出口。

8. 发明授权

US06852284B1 Liquid analysis cartridge 有权
标题翻译：液体分析盒
公开(公告)号：US06852284B1
公开(公告)日：2005-02-08
申请号：US09688055
申请日：2000-10-13
申请人： Mark R. Holl , Floyd Edwards , Robert J. Morff , Gerald L. Klein
发明人： Mark R. Holl , Floyd Edwards , Robert J. Morff , Gerald L. Klein
IPC分类号： B01L3/00 , B01L99/00 , G01N15/14 , G01N15/06 , B01L3/02 , B01L11/00 , G01N33/00 , G01N33/78
CPC分类号： B01L3/502707 , B01F5/0646 , B01F5/0647 , B01F5/0648 , B01F13/0059 , B01L3/502715 , B01L3/502738 , B01L3/502746 , B01L3/502776 , B01L3/565 , B01L2200/025 , B01L2200/027 , B01L2200/0636 , B01L2200/0647 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0627 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/0874 , B01L2300/0883 , B01L2300/0887 , B01L2400/0487 , B01L2400/0655 , G01N15/14 , Y10T156/1064 , Y10T436/11 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/2575
摘要： The present invention provides an apparatus and method for storing a particle-containing liquid. The storage apparatus comprises a microfluidic convoluted flow channel having a plurality of article capture regions. The storage channel is preferably an isotropic spatially periodic channel. Sedimented particles can be resuspended following storage. This invention further provides a microfluidic analysis cartridge having a convoluted storage channel therein. The sample analysis can use optical, electrical, pressure sensitive, or flow sensitive detection. A plurality of analysis channels can be included in a single cartridge. The analysis channels can be joined to reagent inlets for diluents, indicators or lysing agents. A mixing channel can be positioned between the reagent inlet and the analysis region to allow mixing and reaction of the reagent. The cartridge can include additional valves and pumps for flow management. The analysis cartridge can be a self-contained disposable cartridge having an integral waste storage container. This invention further provides a sheath flow assembly. The sheath flow assembly includes a sample channel and first and second sheath fluid channels positioned on either side of and converging with the sample channel. The assembly also includes upper and lower sheath fluid chambers positioned above and below and converging with the sample channel. The flow cartridges of this invention can be formed by molding, machining or etching. In a preferred embodiment they are laminated. This invention further provides a method of fabricating a laminated microfluidic flow device. In the method, flow elements are formed in rigid sheets and abutting surfaces of the sheets are bonded together.
摘要翻译：本发明提供一种用于储存含颗粒的液体的装置和方法。存储装置包括具有多个物品捕捉区域的微流体回旋流道。存储通道优选地是各向同性的空间周期性通道。沉淀的颗粒可以在储存后重新悬浮。本发明还提供一种其中具有回旋存储通道的微流体分析盒。样品分析可以使用光学，电气，压力敏感或流量敏感检测。多个分析通道可以包括在单个盒中。分析通道可以连接到用于稀释剂，指示剂或裂解剂的试剂入口。混合通道可以位于试剂入口和分析区域之间，以允许试剂混合和反应。墨盒可以包括用于流量管理的附加阀和泵。分析盒可以是具有一体式废物储存容器的独立的一次性盒。本发明还提供一种鞘流组件。护套流动组件包括样品通道和位于样品通道两侧并与样品通道收敛的第一和第二鞘液通道。组件还包括位于上方和下方的上部和下部鞘液室，并与样品通道会聚。本发明的流动筒可以通过模制，机加工或蚀刻来形成。在优选实施例中，它们被层压。本发明还提供一种制造层压微流体流动装置的方法。在该方法中，流动元件形成为刚性片材，并且片材的邻接表面粘合在一起。

9. 发明授权

US06830729B1 Sample analysis instrument 有权
标题翻译：样品分析仪
公开(公告)号：US06830729B1
公开(公告)日：2004-12-14
申请号：US09723823
申请日：2000-11-28
申请人： Mark R. Holl , Floyd Edwards , Robert Morff , Gerald L. Klein
发明人： Mark R. Holl , Floyd Edwards , Robert Morff , Gerald L. Klein
IPC分类号： G01N2100
CPC分类号： B01L3/502707 , B01F13/0059 , B01L3/502715 , B01L3/502738 , B01L2200/027 , B01L2200/0636 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2300/0874 , B01L2300/0883 , B01L2300/0887 , B01L2400/0487 , B01L2400/0655 , G01N15/14
摘要： The present invention provides an apparatus and method for storing a particle-containing liquid. The storage apparatus comprises a microfluidic convoluted flow channel having a plurality of particle capture regions. The storage channel is preferably an isotropic spatially periodic channel. Sedimented particles can be resuspended following storage. This invention further provides a microfluidic analysis cartridge having a convoluted storage channel therein. The sample analysis can use optical, electrical, pressure sensitive, or flow sensitive detection. A plurality of analysis channels can be included in a single cartridge. The analysis channels can be joined to reagent inlets for diluents, indicators or lysing agents. A mixing channel can be positioned between the reagent inlet and the analysis region to allow mixing and reaction of the reagent. The cartridge can include additional valves and pumps for flow management. The analysis cartridge can be a self-contained disposable cartridge having an integral waste storage container. This invention further provides a sheath flow assembly. The sheath flow assembly includes a sample channel and first and second sheath fluid channels positioned on either side of and converging with the sample channel. The assembly also includes upper and lower sheath fluid chambers positioned above and below and converging with the sample channel. The flow cartridges of this invention can be formed by molding, machining or etching. In a preferred embodiment they are laminated. This invention further provides a method of fabricating a laminated microfluidic flow device. In the method, flow elements are formed in rigid sheets and abutting surfaces of the sheets are bonded together.
摘要翻译：本发明提供一种用于储存含颗粒的液体的装置和方法。存储装置包括具有多个颗粒捕获区域的微流体回旋流动通道。存储通道优选地是各向同性的空间周期性通道。沉淀的颗粒可以在储存后重新悬浮。本发明还提供一种其中具有回旋存储通道的微流体分析盒。样品分析可以使用光学，电气，压力敏感或流量敏感检测。多个分析通道可以包括在单个盒中。分析通道可以连接到用于稀释剂，指示剂或裂解剂的试剂入口。混合通道可以位于试剂入口和分析区域之间，以允许试剂混合和反应。墨盒可以包括用于流量管理的附加阀和泵。分析盒可以是具有一体式废物储存容器的独立的一次性盒。本发明还提供一种鞘流组件。护套流动组件包括样品通道和位于样品通道两侧并与样品通道收敛的第一和第二鞘液通道。组件还包括位于上方和下方的上部和下部鞘液室，并与样品通道会聚。本发明的流动筒可以通过模制，机加工或蚀刻来形成。在优选实施例中，它们被层压。本发明还提供一种制造层压微流体流动装置的方法。在该方法中，流动元件形成为刚性片材，并且片材的邻接表面粘合在一起。

10. 发明授权

US5948684A Simultaneous analyte determination and reference balancing in reference T-sensor devices 失效
标题翻译：参考T传感器设备中的同时分析物测定和参考平衡
公开(公告)号：US5948684A
公开(公告)日：1999-09-07
申请号：US900926
申请日：1997-07-25
申请人： Bernhard H. Weigl , Mark R. Holl , Diane Zebert , Margaret Kenny , Caicai Wu
发明人： Bernhard H. Weigl , Mark R. Holl , Diane Zebert , Margaret Kenny , Caicai Wu
IPC分类号： G01N31/20 , B01F5/04 , B01F13/00 , B01J19/00 , B01L3/00 , G01N15/10 , G01N15/14 , G01N21/64 , G01N30/00 , G01N30/38 , G01N30/60 , G01N30/74 , G01N35/00 , G01N35/08 , G01N37/00
CPC分类号： B01F13/0093 , B01F13/0059 , B01F5/0403 , B01J19/0093 , B01L3/502776 , G01N15/10 , G01N15/1484 , G01N30/0005 , B01L2200/0636 , B01L2200/148 , B01L2300/0816 , B01L2400/0406 , B01L2400/0415 , B01L2400/0457 , B01L2400/0487 , G01N15/14 , G01N15/1404 , G01N2035/00237 , G01N30/38 , G01N30/6095 , G01N30/74 , G01N35/085 , Y10T436/117497 , Y10T436/118339 , Y10T436/25375 , Y10T436/2575
摘要： A reference T-sensor system is provided for detecting the presence and/or measuring the concentration of analyte particles in a sample stream. The system includes: a) a laminar flow channel; b) three or more inlet means in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may include an indicator substance which indicates the presence of the analyte particles, (2) the sample stream, and (3) a reference stream, which can be a control stream and/or internal standard stream; c) wherein the laminar flow channel has a depth and/or width sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to form a detection area; and d) outlet means for conducting the streams out of the laminar flow channel preferably to form a single mixed stream. Also provided are methods of using the device to improve accuracy and increase convenience of measuring the concentration of analytes in a sample. Monitoring reference stream(s) in laminar flow with sample stream(s) allows for calibration or for monitoring or correcting for the effects of experimental conditions which might otherwise degrade accuracy. A reference stream which serves as both a control stream and an internal standard stream is convenient and economical. The device of this invention also needs less frequent calibration than previously-known fluid analysis devices because the devices and methods of this invention provide for continuous and simultaneous quality control and internal standardization.
摘要翻译：提供了一种参考T传感器系统，用于检测样品流中分析物颗粒的存在和/或测量浓度。该系统包括：a）层流通道; b）与层流通道流体连接的三个或更多个入口装置，用于分别导入层流通道（1）中，指示物流可包括指示物质，其指示分析物颗粒的存在，（2）样品流，和（3）参考流，其可以是控制流和/或内部标准流; c）其中层流通道具有足够小的深度和/或宽度以允许流的层流，并且具有足以允许分析物的颗粒扩散到指示物流中以形成检测区域的长度; 以及d）用于将流引导出层流通道的出口装置优选地形成单一混合流。还提供了使用该装置来提高测量样品中分析物浓度的准确性和增加方便性的方法。用样品流监测层流中的参考流允许校准或监测或校正实际条件的影响，否则可能会降低准确度。用作控制流和内部标准流的参考流是方便和经济的。由于本发明的装置和方法提供连续和同时的质量控制和内部标准化，本发明的装置还需要比先前知道的流体分析装置更少的校准。

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