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    • 4. 发明申请
    • METHODS FOR AMPLIFYING HEPATITIS C VIRUS NUCLEIC ACIDS
    • 用于放大丙型肝炎病毒核酸的方法
    • US20120129155A1
    • 2012-05-24
    • US13186985
    • 2011-07-20
    • Ann Dak-Yee KwongJames Daniel FrantzDouglas John BartelsChao LinBenjamin ShamesSheila SeepersaudJudith A. LippkeTara Lynn KiefferYi ZhouEileen Z. ZhangJames C. Sullivan
    • Ann Dak-Yee KwongJames Daniel FrantzDouglas John BartelsChao LinBenjamin ShamesSheila SeepersaudJudith A. LippkeTara Lynn KiefferYi ZhouEileen Z. ZhangJames C. Sullivan
    • C12Q1/70C12P19/34
    • C12Q1/707C12Q1/6883
    • A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
    • 在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段,其中第一阶段PCR使用第一外部引物 和第二外引物,第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO:2所示的核苷酸序列; 或SEQ ID NO:9,其中任选地1,2或3个核苷酸是比SEQ ID NO:9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO:3或4所示的核苷酸序列; 或如SEQ ID NO:10或11所示的核苷酸序列,其中任选1,2或3个核苷酸是与SEQ ID NO:10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO:5所示; 或SEQ ID NO:12,其中任选地1,2或3个核苷酸是与SEQ ID NO:12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO:6或7所示的核苷酸序列 ; 或如SEQ ID NO:13或14所示的核苷酸序列,其中任选地1,2或3个核苷酸是与SEQ ID NO:13和14相同的其它核苷酸。
    • 6. 发明授权
    • Methods for amplifying hepatitis C virus nucleic acids
    • 扩增丙型肝炎病毒核酸的方法
    • US08679740B2
    • 2014-03-25
    • US13186985
    • 2011-07-20
    • Ann Dak-Yee KwongJames Daniel FrantzDouglas John BartelsChao LinBenjamin ShamesSheila SeepersaudJudith A. LippkeTara Lynn KiefferYi ZhouEileen Z. ZhangJames C. Sullivan
    • Ann Dak-Yee KwongJames Daniel FrantzDouglas John BartelsChao LinBenjamin ShamesSheila SeepersaudJudith A. LippkeTara Lynn KiefferYi ZhouEileen Z. ZhangJames C. Sullivan
    • C12Q1/70C12Q1/68C12P19/34
    • C12Q1/707C12Q1/6883
    • A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
    • 在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段,其中第一阶段PCR使用第一外部引物 和第二外引物,第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO:2所示的核苷酸序列; 或SEQ ID NO:9,其中任选地1,2或3个核苷酸是比SEQ ID NO:9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO:3或4所示的核苷酸序列; 或如SEQ ID NO:10或11所示的核苷酸序列,其中任选1,2或3个核苷酸是与SEQ ID NO:10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO:5所示; 或SEQ ID NO:12,其中任选地1,2或3个核苷酸是与SEQ ID NO:12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO:6或7所示的核苷酸序列 ; 或如SEQ ID NO:13或14所示的核苷酸序列,其中任选地1,2或3个核苷酸是与SEQ ID NO:13和14相同的其它核苷酸。