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    • 1. 发明申请
    • METHODS FOR AMPLIFYING HEPATITIS C VIRUS NUCLEIC ACIDS
    • 用于放大丙型肝炎病毒核酸的方法
    • US20120129155A1
    • 2012-05-24
    • US13186985
    • 2011-07-20
    • Ann Dak-Yee KwongJames Daniel FrantzDouglas John BartelsChao LinBenjamin ShamesSheila SeepersaudJudith A. LippkeTara Lynn KiefferYi ZhouEileen Z. ZhangJames C. Sullivan
    • Ann Dak-Yee KwongJames Daniel FrantzDouglas John BartelsChao LinBenjamin ShamesSheila SeepersaudJudith A. LippkeTara Lynn KiefferYi ZhouEileen Z. ZhangJames C. Sullivan
    • C12Q1/70C12P19/34
    • C12Q1/707C12Q1/6883
    • A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
    • 在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段,其中第一阶段PCR使用第一外部引物 和第二外引物,第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO:2所示的核苷酸序列; 或SEQ ID NO:9,其中任选地1,2或3个核苷酸是比SEQ ID NO:9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO:3或4所示的核苷酸序列; 或如SEQ ID NO:10或11所示的核苷酸序列,其中任选1,2或3个核苷酸是与SEQ ID NO:10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO:5所示; 或SEQ ID NO:12,其中任选地1,2或3个核苷酸是与SEQ ID NO:12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO:6或7所示的核苷酸序列 ; 或如SEQ ID NO:13或14所示的核苷酸序列,其中任选地1,2或3个核苷酸是与SEQ ID NO:13和14相同的其它核苷酸。
    • 2. 发明授权
    • Methods for amplifying hepatitis C virus nucleic acids
    • 扩增丙型肝炎病毒核酸的方法
    • US08679740B2
    • 2014-03-25
    • US13186985
    • 2011-07-20
    • Ann Dak-Yee KwongJames Daniel FrantzDouglas John BartelsChao LinBenjamin ShamesSheila SeepersaudJudith A. LippkeTara Lynn KiefferYi ZhouEileen Z. ZhangJames C. Sullivan
    • Ann Dak-Yee KwongJames Daniel FrantzDouglas John BartelsChao LinBenjamin ShamesSheila SeepersaudJudith A. LippkeTara Lynn KiefferYi ZhouEileen Z. ZhangJames C. Sullivan
    • C12Q1/70C12Q1/68C12P19/34
    • C12Q1/707C12Q1/6883
    • A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
    • 在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段,其中第一阶段PCR使用第一外部引物 和第二外引物,第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO:2所示的核苷酸序列; 或SEQ ID NO:9,其中任选地1,2或3个核苷酸是比SEQ ID NO:9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO:3或4所示的核苷酸序列; 或如SEQ ID NO:10或11所示的核苷酸序列,其中任选1,2或3个核苷酸是与SEQ ID NO:10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO:5所示; 或SEQ ID NO:12,其中任选地1,2或3个核苷酸是与SEQ ID NO:12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO:6或7所示的核苷酸序列 ; 或如SEQ ID NO:13或14所示的核苷酸序列,其中任选地1,2或3个核苷酸是与SEQ ID NO:13和14相同的其它核苷酸。
    • 10. 发明授权
    • User interface for handwriting inputs
    • 手写输入用户界面
    • US08836667B2
    • 2014-09-16
    • US13222591
    • 2011-08-31
    • Juan DaiGong XinYi ZhouLiang Chen
    • Juan DaiGong XinYi ZhouLiang Chen
    • G06F3/045
    • G06K9/00436G06F3/018G06F3/04883
    • A method, medium, device, and user interface are provided for receiving a handwriting input and for presenting prediction candidates based on the handwriting input. A pen down event is detected in a writing panel of a user interface. The writing panel is expanded to overlie function keys and a candidate window of the user interface such that those features are made inaccessible for inadvertent selections thereof. Upon detection of a pen up event the writing panel is decreased in size to again reveal the features. Recognition candidates are identified based on the handwriting input. Prediction candidates are also identified based on a highest ranking recognition candidate and are presented in combination with the highest ranking recognition candidate. Prediction candidates are thereby identifiable with the recognition candidate with which they are associated and selection of a prediction candidate and recognition candidate may be performed in single selection input.
    • 提供了一种方法,媒体,设备和用户界面,用于接收手写输入并基于手写输入呈现预测候选。 在用户界面的书写面板中检测到笔直事件。 写入面板被扩展以覆盖功能键和用户界面的候选窗口,使得这些特征使得无意地进行选择。 当检测到笔触事件时,写入面板的尺寸减小以再次显示特征。 基于手写输入识别识别候选者。 预测候选者也基于最高排名识别候选来确定,并且与最高排名识别候选者一起呈现。 因此,预测候选者可以与其相关联的识别候选者识别,并且可以在单选输入中执行预测候选和识别候选的选择。