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    • 5. 发明专利
    • Gel electrophoretic system and method for focusing biomolecule band
    • 凝胶电泳系统和聚焦生物分子带的方法
    • JP2010237227A
    • 2010-10-21
    • JP2010168508
    • 2010-07-27
    • Palo Alto Research Center Incパロ・アルト・リサーチ・センター・インコーポレーテッドPalo Alto Research Center Incorporated
    • LEAN MENG HFITCH JOHN SPREAS BRYAN TBRUCE RICHARD HFRANCISCO E TORRESBEN HSIEH HVOLKEL ARMIN RELROD SCOTT APEETERS ERICCHABINYC MICHAEL L
    • G01N27/447C08F2/58
    • G01N27/44795G01N27/44713
    • PROBLEM TO BE SOLVED: To focus molecules into a narrow band, in electrophoresis. SOLUTION: A gel electrophoretic system 10 for separation includes: an electrophoretic cell comprising a first end, a second end, and a gel medium extending between the first and second ends; an electrode grid disposed in close proximity to the gel which includes a plurality of closely spaced parallel electrodes 32, the electrode grid being disposed with respect to the cell such that the electrodes 32 extend in a direction generally perpendicular to the longitudinal direction of the cell; and voltage controllers 40 and 50 for providing multi-phase electrical signals. With the voltage controllers 40 and 50 electrically connected to the electrode grid, a multi-phase electrical signal which is a first output is provided from the voltage controller 40 to the first end while a multi-phase electrical signal which is a second output is provided from the voltage controller 50 to the second end. The multi-phase electrical signal which is the first output passes from a first region of the electrode grid to a second region of the electrode grid while the multi-phase electrical signal which is the second output passes from the second region of the electrode grid to the first region of the electrode grid. COPYRIGHT: (C)2011,JPO&INPIT
    • 要解决的问题:在电泳中将分子聚焦成窄带。 解决方案:用于分离的凝胶电泳系统10包括:电泳单元,包括在第一和第二端之间延伸的第一端,第二端和凝胶介质; 电极栅极,其布置在紧密的凝胶处,包括多个紧密间隔开的平行电极32,电极栅格相对于电池设置,使得电极32沿大致垂直于电池的纵向的方向延伸; 以及用于提供多相电信号的电压控制器40和50。 当电压控制器40和50电连接到电极栅极时,作为第一输出的多相电信号从电压控制器40提供到第一端,同时提供作为第二输出的多相电信号 从电压控制器50到第二端。 作为第一输出的多相电信号从电极栅格的第一区域通过电极栅极的第二区域,而作为第二输出的多相电信号从电极栅极的第二区域通过 电极网格的第一个区域。 版权所有(C)2011,JPO&INPIT