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1. 发明申请

WO2010011607A1 ADP DETECTION BASED LUMINESCENT PHOSPHOTRANSFERASE OR ATP HYDROLASE ASSAY 审中-公开
标题翻译：基于ADP检测的LUMINESCENT PHOSPHOTRANSFERASE或ATP水解测定
公开(公告)号：WO2010011607A1
公开(公告)日：2010-01-28
申请号：PCT/US2009/051170
申请日：2009-07-20
申请人： PROMEGA CORPORATION , GOUELI, Said, A. , ZEGZOUTI, Hicham
发明人： GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： G01N33/53
CPC分类号： C12Q1/008
摘要： The invention provides provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP.
摘要翻译：本发明提供了确定或检测酶的活性的组合物和方法，包括磷酸转移酶例如激酶（例如蛋白质，脂质和糖激酶）和ATP水解酶例如使用ATP作为底物的ATP酶，例如HSP90，通过监测ADP的变化，形成ADP作为产品。

2. 发明申请

WO2016057084A1 BIOLUMINESCENT SUCCINATE DETECTION ASSAY 审中-公开
标题翻译：生物汞成功检测测定
公开(公告)号：WO2016057084A1
公开(公告)日：2016-04-14
申请号：PCT/US2015/031445
申请日：2015-05-18
申请人： PROMEGA CORPORATION
发明人： ALVES, Juliano , GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： C12Q1/527 , G01N33/573
CPC分类号： C12Q1/66 , C12Q1/25 , C12Q1/26 , C12Q1/48 , G01N2333/9015 , G01N2333/90245 , G01N2333/91194 , G01N2333/91235
摘要： Provided herein are methods for detecting and quantifying succinate in a sample. Also provided are methods for detecting and quantifying 2-oxoglutarate oxygenase enzyme and/or 2-oxoglutarate oxygenase activity in a sample and methods for screening for modulators of 2-oxoglutarate oxygenase activity.
摘要翻译：本文提供了用于检测和定量样品中琥珀酸盐的方法。还提供了用于检测和定量样品中2-氧戊二烯加氧酶和/或2-氧戊二烯加氧酶活性的方法以及用于筛选2-氧戊二酸加氧酶活性调节剂的方法。

3. 发明申请

WO2014152279A1 METHOD FOR QUANTIFYING 5-HYDROXYMETHYLCYTOSINE 审中-公开
标题翻译：定量分析5-羟基甲基吗啉的方法
公开(公告)号：WO2014152279A1
公开(公告)日：2014-09-25
申请号：PCT/US2014/027155
申请日：2014-03-14
申请人： PROMEGA CORPORATION
发明人： ZEGZOUTI, Hicham , ENGEL, Laurie , GOUELI, Said, A.
IPC分类号： G01N33/50 , C12Q1/68
CPC分类号： C12Q1/66 , C12Q1/48 , C12Q1/485 , C12Q1/6827 , C12Y204/01028 , C12Y207/04022 , G01N2333/90245 , C12Q2563/103
摘要： Provided herein are methods for detecting and quantifying 5-hydroxymethylated cytosine bases in a DNA molecule.
摘要翻译：本文提供了用于检测和定量DNA分子中的5-羟甲基化胞嘧啶碱基的方法。

4. 发明申请

WO2014151736A1 ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION 审中-公开
标题翻译：通过结构补充激活生物光合作用
公开(公告)号：WO2014151736A1
公开(公告)日：2014-09-25
申请号：PCT/US2014/026354
申请日：2014-03-13
申请人： PROMEGA CORPORATION
发明人： DIXON, Andrew, S. , ENCELL, Lance , HALL, Mary , WOOD, Keith , WOOD, Monika , SCHWINN, Marie , BINKOWSKI, Brock, F. , ZEGZOUTI, Hicham , NATH, Nidhi , MONDAL, Subhanjan , GOUELI, Said , MEISENHEIMER, Poncho , KIRKLAND, Thomas , UNCH, James , PULUKKUNAT, Dileep, K. , ROBERS, Matthew , DART, Melanie , MACHLEIDT, Thomas
IPC分类号： C07K14/435
CPC分类号： G01N33/533 , A61K51/08 , C07K7/08 , C07K14/43509 , C12N9/0069 , C12Q1/66 , G01N33/542 , G01N33/581
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide. Biological processes rely on covalent and non-covalent interactions between molecules, macromolecules and molecular complexes. In order to understand such processes, and to develop techniques and compounds to manipulate them for research, clinical and other practical applications, it is necessary to have tools available to detect and monitor these interactions.
摘要翻译：本文提供了用于从两个或多个非发光（例如，基本上不发光的）肽和/或多肽单元组装生物发光复合物的组合物和方法。特别地，通过与另一种互补的非发光肽的结构互补，赋予非发光多肽的生物发光活性。生物过程依赖于分子，大分子和分子复合物之间的共价和非共价相互作用。为了理解这些过程，并开发用于研究，临床和其他实际应用的技术和化合物，需要有可用于检测和监测这些相互作用的工具。

5. 发明申请

WO2013063563A2 METHODS FOR DETECTING ADENOSINE MONOPHOSPHATE IN BIOLOGICAL SAMPLES 审中-公开
标题翻译：检测生物样品中腺苷一磷酸的方法
公开(公告)号：WO2013063563A2
公开(公告)日：2013-05-02
申请号：PCT/US2012/062397
申请日：2012-10-29
申请人： PROMEGA CORPORATION
发明人： GOUELI, Said, A. , HSIAO, Kevin , ZEGZOUTI, Hicham
IPC分类号： G01N33/53
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485
摘要： The present invention provides compositions and methods to detect and/or determine the amount and/or presence of adenosine monophosphate (AMP) in biological samples. The method comprises converting substantially all AMP in the solution to adenosine diphosphate (ADP) using a first enzyme, suitably polyphosphate: AMP phosphotransferase (PAP), that is capable of converting AMP to ADP; converting the ADP in the solution to adenosine triphosphate (ATP) using a second enzyme, suitably adenylate kinase (AK), that is capable of converting ADP to ATP; determining the amount of the ATP produced using a bioluminescent reaction utilizing a luciferase enzyme and a substrate for the luciferase enzyme; and using the amount of ATP produced to determine the amount of AMP present in the original solution.
摘要翻译：本发明提供用于检测和/或确定生物样品中腺苷一磷酸（AMP）的量和/或存在的组合物和方法。该方法包括使用能够将AMP转化为ADP的第一种酶，适当的多磷酸盐：AMP磷酸转移酶（PAP）将基本上所有溶液中的所有AMP转化为二磷酸腺苷（ADP）; 使用能够将ADP转化为ATP的适当腺苷酸激酶（AK）的第二种酶将溶液中的ADP转化为三磷酸腺苷（ATP）; 使用荧光素酶和荧光素酶底物的生物发光反应测定产生的ATP的量; 并使用产生的ATP的量来确定原始溶液中存在的AMP的量。

6. 发明公开

EP3204509A1 BIOLUMINESCENT SUCCINATE DETECTION ASSAY 有权
标题翻译：生物发光琥珀酸盐检测分析
公开(公告)号：EP3204509A1
公开(公告)日：2017-08-16
申请号：EP15727175.0
申请日：2015-05-18
申请人： Promega Corporation
发明人： ALVES, Juliano , GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： C12Q1/527 , G01N33/573
CPC分类号： C12Q1/66 , C12Q1/25 , C12Q1/26 , C12Q1/48 , G01N2333/9015 , G01N2333/90245 , G01N2333/91194 , G01N2333/91235
摘要： Provided herein are methods for detecting and quantifying succinate in a sample. Also provided are methods for detecting and quantifying 2-oxoglutarate oxygenase enzyme and/or 2-oxoglutarate oxygenase activity in a sample and methods for screening for modulators of 2-oxoglutarate oxygenase activity.
摘要翻译：本文提供了用于检测和定量样品中的琥珀酸盐的方法。还提供了用于检测和定量样品中的2-氧代戊二酸加氧酶和/或2-酮戊二酸加氧酶活性的方法以及用于筛选2-酮戊二酸加氧酶活性的调节剂的方法。

7. 发明授权

EP2326953B1 ADP DETECTION BASED LUMINESCENT PHOSPHOTRANSFERASE OR ATP HYDROLASE ASSAY 有权
标题翻译：基于ADP检测的发光磷酸转移酶或ATP水解酶测定
公开(公告)号：EP2326953B1
公开(公告)日：2018-03-21
申请号：EP09790643.2
申请日：2009-07-20
申请人： Promega Corporation
发明人： GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： G01N33/53
CPC分类号： C12Q1/008
摘要： The disclosure provides compositions and methods to determine or detect ADP or the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP.

8. 发明授权

EP2771480B1 METHODS FOR DETECTING ADENOSINE MONOPHOSPHATE IN BIOLOGICAL SAMPLES 有权
标题翻译：检测方法的磷酸腺苷生物样品
公开(公告)号：EP2771480B1
公开(公告)日：2016-12-14
申请号：EP12791022.2
申请日：2012-10-29
申请人： Promega Corporation
发明人： GOUELI, Said, A. , HSIAO, Kevin , ZEGZOUTI, Hicham
IPC分类号： C12Q1/44 , C12Q1/48 , G01N33/53
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485

9. 发明公开

EP3783011A1 ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION 有权
公开(公告)号：EP3783011A1
公开(公告)日：2021-02-24
申请号：EP20197758.4
申请日：2014-03-13
申请人： Promega Corporation
发明人： DIXON, Andrew S. , ENCELL, Lance P. , HALL, Mary P. , WOOD, Keith V. , WOOD, Monika G. , SCHWINN, Marie , BINKOWSKI, Brock , ZEGZOUTI, Hicham , NATH, Nidhi , MONDAL, Subhanjan , GOUELI, Said , MEISENHEIMER, Poncho , KIRKLAND, Thomas , UNCH, James , PULUKKUNAT, Dileep K. , ROBERS, Matthew B. , DART, Melanie , MACHLEIDT, Thomas
IPC分类号： C07K14/435 , G01N33/50
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

10. 发明授权

EP3204509B1 BIOLUMINESCENT SUCCINATE DETECTION ASSAY 有权
公开(公告)号：EP3204509B1
公开(公告)日：2019-07-10
申请号：EP15727175.0
申请日：2015-05-18
申请人： Promega Corporation
发明人： ALVES, Juliano , GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： C12Q1/527 , G01N33/573

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