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1. 发明申请

WO1995023612A1 QUANTITATION OF INDIVIDUAL PROTEIN KINASE ACTIVITY 审中-公开
标题翻译：个体蛋白激酶活性的定量
公开(公告)号：WO1995023612A1
公开(公告)日：1995-09-08
申请号：PCT/US1995002856
申请日：1995-03-06
申请人： PROMEGA CORPORATION
发明人： PROMEGA CORPORATION , GOUELI, Said, A.
IPC分类号： A61K38/45
CPC分类号： C07K7/06 , C07K7/08 , C12Q1/48 , C12Q1/485
摘要： A method of quantitating the activity of a selected protein kinase on a peptide substrate is provided. The peptide substrate is conjugated to a binding compound. The modified peptide substrate is then added to a solution containing the selected protein kinase. The protein kinase and the peptide are incubated along with a label for sufficient time to form a modified peptide product having the binding compound and the label. The modified peptide product is then bound to a matrix having a high binding compound affinity. The bound peptide is then washed and the activity of the protein kinase is measured, as shown in the figure. Also provided is a kit for the stated method.
摘要翻译：提供了在肽底物上定量选择的蛋白激酶的活性的方法。将肽底物与结合化合物缀合。然后将修饰的肽底物加入到含有选择的蛋白激酶的溶液中。蛋白激酶和肽与标签一起温育足够的时间以形成具有结合化合物和标记的修饰肽产物。然后将修饰的肽产物与具有高结合化合物亲和力的基质结合。然后洗涤结合的肽，并测量蛋白激酶的活性，如图所示。还提供了所述方法的试剂盒。

2. 发明申请

WO2004009540A2 METHODS AND KITS FOR TRANSFERASES 审中-公开
标题翻译：用于转移的方法和工具
公开(公告)号：WO2004009540A2
公开(公告)日：2004-01-29
申请号：PCT/US2003/022315
申请日：2003-07-17
申请人： PROMEGA CORPORATION , GOUELI, Said, A. , BULLEIT, Robert, F.
发明人： GOUELI, Said, A. , BULLEIT, Robert, F.
IPC分类号： C07D
CPC分类号： G01N33/542 , C07K7/06 , C07K7/08 , C07K2319/60 , C12Q1/37 , C12Q1/42 , C12Q1/48 , C12Q1/485
摘要： A method for detecting transferase activity of a sample includes contacting the sample with a substrate and at least one of a phosphate group donor and a phosphate group acceptor. The substrate includes a reporter compound and amino acids. A peptidase is added that cleaves a non-phosphorylated substrate at a first rate and a phosphorylated substrate and a second rate. The output of the reporter compound is detected. In a preferred embodiment, the transferase activity detected is a kinase activity. In another preferred embodiment, the transferase activity detected is a phosphatase activity. Also provided is a method of screening for alterations in a transferase reaction. Kits and peptide substrate are also provided for carrying out at least one of the methods of the invention.
摘要翻译：用于检测样品的转移酶活性的方法包括使样品与底物和磷酸基供体和磷酸酯基受体中的至少一种接触。底物包括报告化合物和氨基酸。加入肽酶，其以第一速率和磷酸化底物和第二速率切割非磷酸化底物。检测报告化合物的输出。在优选的实施方案中，所检测的转移酶活性是激酶活性。在另一个优选的实施方案中，所检测的转移酶活性是磷酸酶活性。还提供了筛选转移酶反应中的改变的方法。还提供了用于实施本发明的至少一种方法的试剂盒和肽底物。

3. 发明申请

WO2010011607A1 ADP DETECTION BASED LUMINESCENT PHOSPHOTRANSFERASE OR ATP HYDROLASE ASSAY 审中-公开
标题翻译：基于ADP检测的LUMINESCENT PHOSPHOTRANSFERASE或ATP水解测定
公开(公告)号：WO2010011607A1
公开(公告)日：2010-01-28
申请号：PCT/US2009/051170
申请日：2009-07-20
申请人： PROMEGA CORPORATION , GOUELI, Said, A. , ZEGZOUTI, Hicham
发明人： GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： G01N33/53
CPC分类号： C12Q1/008
摘要： The invention provides provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP.
摘要翻译：本发明提供了确定或检测酶的活性的组合物和方法，包括磷酸转移酶例如激酶（例如蛋白质，脂质和糖激酶）和ATP水解酶例如使用ATP作为底物的ATP酶，例如HSP90，通过监测ADP的变化，形成ADP作为产品。

4. 发明申请

WO2016057084A1 BIOLUMINESCENT SUCCINATE DETECTION ASSAY 审中-公开
标题翻译：生物汞成功检测测定
公开(公告)号：WO2016057084A1
公开(公告)日：2016-04-14
申请号：PCT/US2015/031445
申请日：2015-05-18
申请人： PROMEGA CORPORATION
发明人： ALVES, Juliano , GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： C12Q1/527 , G01N33/573
CPC分类号： C12Q1/66 , C12Q1/25 , C12Q1/26 , C12Q1/48 , G01N2333/9015 , G01N2333/90245 , G01N2333/91194 , G01N2333/91235
摘要： Provided herein are methods for detecting and quantifying succinate in a sample. Also provided are methods for detecting and quantifying 2-oxoglutarate oxygenase enzyme and/or 2-oxoglutarate oxygenase activity in a sample and methods for screening for modulators of 2-oxoglutarate oxygenase activity.
摘要翻译：本文提供了用于检测和定量样品中琥珀酸盐的方法。还提供了用于检测和定量样品中2-氧戊二烯加氧酶和/或2-氧戊二烯加氧酶活性的方法以及用于筛选2-氧戊二酸加氧酶活性调节剂的方法。

5. 发明申请

WO2014152279A1 METHOD FOR QUANTIFYING 5-HYDROXYMETHYLCYTOSINE 审中-公开
标题翻译：定量分析5-羟基甲基吗啉的方法
公开(公告)号：WO2014152279A1
公开(公告)日：2014-09-25
申请号：PCT/US2014/027155
申请日：2014-03-14
申请人： PROMEGA CORPORATION
发明人： ZEGZOUTI, Hicham , ENGEL, Laurie , GOUELI, Said, A.
IPC分类号： G01N33/50 , C12Q1/68
CPC分类号： C12Q1/66 , C12Q1/48 , C12Q1/485 , C12Q1/6827 , C12Y204/01028 , C12Y207/04022 , G01N2333/90245 , C12Q2563/103
摘要： Provided herein are methods for detecting and quantifying 5-hydroxymethylated cytosine bases in a DNA molecule.
摘要翻译：本文提供了用于检测和定量DNA分子中的5-羟甲基化胞嘧啶碱基的方法。

6. 发明申请

WO2013063563A2 METHODS FOR DETECTING ADENOSINE MONOPHOSPHATE IN BIOLOGICAL SAMPLES 审中-公开
标题翻译：检测生物样品中腺苷一磷酸的方法
公开(公告)号：WO2013063563A2
公开(公告)日：2013-05-02
申请号：PCT/US2012/062397
申请日：2012-10-29
申请人： PROMEGA CORPORATION
发明人： GOUELI, Said, A. , HSIAO, Kevin , ZEGZOUTI, Hicham
IPC分类号： G01N33/53
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485
摘要： The present invention provides compositions and methods to detect and/or determine the amount and/or presence of adenosine monophosphate (AMP) in biological samples. The method comprises converting substantially all AMP in the solution to adenosine diphosphate (ADP) using a first enzyme, suitably polyphosphate: AMP phosphotransferase (PAP), that is capable of converting AMP to ADP; converting the ADP in the solution to adenosine triphosphate (ATP) using a second enzyme, suitably adenylate kinase (AK), that is capable of converting ADP to ATP; determining the amount of the ATP produced using a bioluminescent reaction utilizing a luciferase enzyme and a substrate for the luciferase enzyme; and using the amount of ATP produced to determine the amount of AMP present in the original solution.
摘要翻译：本发明提供用于检测和/或确定生物样品中腺苷一磷酸（AMP）的量和/或存在的组合物和方法。该方法包括使用能够将AMP转化为ADP的第一种酶，适当的多磷酸盐：AMP磷酸转移酶（PAP）将基本上所有溶液中的所有AMP转化为二磷酸腺苷（ADP）; 使用能够将ADP转化为ATP的适当腺苷酸激酶（AK）的第二种酶将溶液中的ADP转化为三磷酸腺苷（ATP）; 使用荧光素酶和荧光素酶底物的生物发光反应测定产生的ATP的量; 并使用产生的ATP的量来确定原始溶液中存在的AMP的量。

7. 发明申请

WO2007067557A2 METHODS FOR CYCLIC NUCLEOTIDE DETERMINATION 审中-公开
标题翻译：循环核苷酸测定方法
公开(公告)号：WO2007067557A2
公开(公告)日：2007-06-14
申请号：PCT/US2006/046431
申请日：2006-12-06
申请人： PROMEGA CORPORATION , GOUELI, Said, A. , HSAIO, Kuei-Hsuan , KUMAR, Meera , VIDUGIRIENE, Jolanta
发明人： GOUELI, Said, A. , HSAIO, Kuei-Hsuan , KUMAR, Meera , VIDUGIRIENE, Jolanta
IPC分类号： G01N33/53 , C12Q1/66 , C12Q1/48
CPC分类号： C12Q1/485 , C12Q1/008 , C12Q1/44 , C12Q1/527 , C12Q1/66 , G01N33/74 , G01N2333/16 , G01N2333/726 , G01N2333/916 , G01N2333/988
摘要： The present invention relates in general to cellular analysis tools and more particularly to methods for detecting or determining cyclic nucleotide concentrations in samples.
摘要翻译：本发明一般涉及细胞分析工具，更具体地涉及用于检测或确定样品中环核苷酸浓度的方法。

8. 发明申请

WO2004023098A2 METHOD FOR DETECTING TRANSFERASE ENZYMATIC ACTIVITY 审中-公开
标题翻译：检测转移酶活性的方法
公开(公告)号：WO2004023098A2
公开(公告)日：2004-03-18
申请号：PCT/US2003/027854
申请日：2003-09-05
申请人： PROMEGA CORPORATION , SOMBERG, Richard , GOUELI, Said, A.
发明人： SOMBERG, Richard , GOUELI, Said, A.
IPC分类号： G01N
CPC分类号： C12Q1/66 , C12Q1/485
摘要： Methods and kits for detecting transferase activity in a sample by measuring ATP using a composition comprising an ATP-dependent bioluminescence-generating enzyme, a luminogenic molecule and one or more transferase quenching agents.
摘要翻译：通过使用包含ATP依赖性生物发光生成酶，发光分子和一种或多种转移酶猝灭剂的组合物测量ATP来检测样品中转移酶活性的方法和试剂盒。

9. 发明授权

EP3204509B1 BIOLUMINESCENT SUCCINATE DETECTION ASSAY 有权
公开(公告)号：EP3204509B1
公开(公告)日：2019-07-10
申请号：EP15727175.0
申请日：2015-05-18
申请人： Promega Corporation
发明人： ALVES, Juliano , GOUELI, Said, A. , ZEGZOUTI, Hicham
IPC分类号： C12Q1/527 , G01N33/573

10. 发明公开

EP2771480A2 METHODS FOR DETECTING ADENOSINE MONOPHOSPHATE IN BIOLOGICAL SAMPLES 有权
标题翻译：检测方法的磷酸腺苷生物样品
公开(公告)号：EP2771480A2
公开(公告)日：2014-09-03
申请号：EP12791022.2
申请日：2012-10-29
申请人： Promega Corporation
发明人： GOUELI, Said, A. , HSIAO, Kevin , ZEGZOUTI, Hicham
IPC分类号： C12Q1/48 , G01N33/53
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485
摘要： The present invention provides compositions and methods to detect and/or determine the amount and/or presence of adenosine monophosphate (AMP) in biological samples. The method comprises converting substantially all AMP in the solution to adenosine diphosphate (ADP) using a first enzyme, suitably polyphosphate: AMP phosphotransferase (PAP), that is capable of converting AMP to ADP; converting the ADP in the solution to adenosine triphosphate (ATP) using a second enzyme, suitably adenylate kinase (AK), that is capable of converting ADP to ATP; determining the amount of the ATP produced using a bioluminescent reaction utilizing a luciferase enzyme and a substrate for the luciferase enzyme; and using the amount of ATP produced to determine the amount of AMP present in the original solution.

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