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1. 发明申请

WO2006107312A1 NANOSENSORS 审中-公开
公开(公告)号：WO2006107312A1
公开(公告)日：2006-10-12
申请号：PCT/US2005/020974
申请日：2005-06-15
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , WANG, Wayne , CHEN, Chuo , LIN, Keng-hui , FANG, Ying , LIEBER, Charles, M.
发明人： WANG, Wayne , CHEN, Chuo , LIN, Keng-hui , FANG, Ying , LIEBER, Charles, M.
IPC分类号： G01N27/414 , G01N33/543
CPC分类号： B82Y15/00 , G01N27/4146 , G01N33/54373
摘要： The present invention generally relates to nanoscale wires for use in determining analytes suspected to be present in a sample, especially in connection with determining information about a sample containing, or suspected of containing, two or more analytes. For example, the invention can involve a competitive, uncompetitive, or non-competitive binding assay including a nanoscale wire to a sample containing a species able to interact with the retain entity to produce a product, where the sample also contains or is suspected of containing a second species able to interact with the reaction entity to prevent production of the product resulting from interaction of the first species and the reaction entity. Based upon determination of production of the product, determination of the second species in the sample can be made. In one set of embodiments, nanoscale wires can be used that have been functionalized at their surface, and/or in close proximity to their surface, for example, by immobilizing a protein or an enzyme relative to the nanoscale wire. Functionalization may permit interaction of the nanoscale wire with various analytes, and such interaction may induce a determinable change in a property of the nanoscale wire. Determination of two or more analytes, o one analyte and the suspected presence of another analyte can involve, for example, binding species to a protein or an enzyme immobilized relative to the nanoscale wire. Other aspects of the invention include assays, sensors, detectors, and/or other devices that include functionalized nanoscale wires, methods of making and/or using functionalized nanoscale wires (for example, in drug screening or high throughput screening) and the like.
摘要翻译：本发明一般涉及用于确定疑似存在于样品中的分析物的纳米尺度丝，特别是关于确定含有或怀疑含有两种或多种分析物的样品的信息。例如，本发明可涉及竞争性的，无竞争性的或非竞争性的结合测定，其包括纳米尺度的丝线，其含有能够与保留实体相互作用以产生产品的样品，其中样品还含有或被怀疑含有能够与反应实体相互作用以防止由第一种和反应实体的相互作用产生的产物的第二种。基于产品生产的确定，可以确定样品中的第二种。在一组实施方案中，可以使用在其表面和/或其表面附近功能化的纳米线，例如通过相对于纳米线固定蛋白质或酶。功能化可以允许纳米尺度线与各种分析物的相互作用，并且这种相互作用可以诱导纳米尺度线的性质的可确定的变化。测定两种或更多种分析物，一种分析物和怀疑存在另一种分析物可涉及例如将物质结合到相对于纳米尺度线固定的蛋白质或酶。本发明的其它方面包括测定，传感器，检测器和/或包括功能化的纳米线的其它器件，制造和/或使用官能化的纳米线（例如在药物筛选或高通量筛选）等的方法。

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