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1. 发明申请

WO2017136204A1 GENETIC LOCI ASSOCIATED WITH BROWN STEM ROT RESISTANCE IN SOYBEAN AND METHODS OF USE 审中-公开
标题翻译：大豆褐斑病抗性遗传分析及利用方法
公开(公告)号：WO2017136204A1
公开(公告)日：2017-08-10
申请号：PCT/US2017/014947
申请日：2017-01-25
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： KYLE, Donald Earl , SHENDELMAN, Joshua Michael , WOODWARD, John Bryan
IPC分类号： A01H1/04 , C12Q1/68
CPC分类号： A01H1/04 , C12Q1/6895 , C12Q2600/156 , C12Q2600/172
摘要： Various methods and compositions are provided for identifying and/or selecting a soybean plant or soybean germplasm with improved resistance to Brown Stem Rot infection. In certain embodiments, the method comprises detecting at least one allele of one or more marker locus within or linked to a QTL associated with Brown Stem Rot resistance. In further embodiments, the method comprises crossing a selected soybean plant with a recurrent soybean parent plant. Further provided herein are marker loci, marker alleles, primers, probes, and kits suitable for identifying and/or selecting soybean plants or soybean germplasms with improved resistance to Brown Stem Rot infection.
摘要翻译：提供了各种方法和组合物用于鉴定和/或选择对褐茎腐病感染具有改善的抗性的大豆植物或大豆种质。在某些实施方案中，该方法包括检测与褐腐病抗性相关的QTL内或与其相关的一个或多个标记基因座的至少一个等位基因。在进一步的实施方案中，该方法包括使选择的大豆植物与复发的大豆亲本植物杂交。本文进一步提供了适用于鉴定和/或选择具有改善的棕色茎腐病抗性的大豆植物或大豆种质的标记基因座，标记等位基因，引物，探针和试剂盒。

2. 发明申请

WO2017106447A1 GENETIC LOCI ASSOCIATED WITH REPRODUCTIVE GROWTH PHENOTYPES IN SOYBEAN AND METHODS OF USE 审中-公开
标题翻译：大豆繁殖生长表型的遗传基因及其使用方法
公开(公告)号：WO2017106447A1
公开(公告)日：2017-06-22
申请号：PCT/US2016/066833
申请日：2016-12-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： KYLE, Donald Earl , RIES, Landon Linn , SHENDELMAN, Joshua , WANG, Hui , WOODWARD, John Bryan , YANG, Ming
IPC分类号： A01H5/10 , C12N15/82
CPC分类号： C12N15/827 , A01H5/10
摘要： Various methods and compositions are provided for identifying and/or selecting a soybean plant or soybean germplasm with one or more preferred or desired reproductive growth phenotypes. In certain embodiments, the method comprises detecting at least one allele of one or more marker locus within or linked to a QTL associated with days to initiation of flowering. In other embodiments, the method additionally comprises detecting at least one allele of one or more marker locus within or linked to a QTL associated with days to maturity. In further embodiments, the method comprises crossing a selected soybean plant with a recurrent soybean parent plant. Further provided herein are marker loci, marker alleles, primers, probes, and kits suitable for identifying and/or selecting soybean plants or soybean germplasms with one or more reproductive growth phenotypes.
摘要翻译：提供了用于鉴定和/或选择具有一种或多种优选或期望的生殖生长表型的大豆植物或大豆种质的各种方法和组合物。在某些实施方案中，该方法包括检测与开花开始的日期相关的QTL内或与其相关的一个或多个标记基因座的至少一个等位基因。在其他实施方案中，该方法另外包括检测与成熟期相关的QTL内或与其相关的一个或多个标记基因座的至少一个等位基因。在进一步的实施方案中，该方法包括使选择的大豆植物与复发的大豆亲本植物杂交。本文进一步提供了适用于鉴定和/或选择具有一种或多种生殖生长表型的大豆植物或大豆种质的标记基因座，标记等位基因，引物，探针和试剂盒。

3. 发明申请

WO2017139544A1 QTLS ASSOCIATED WITH AND METHODS FOR IDENTIFYING LODGING RESISTANCE IN SOYBEAN 审中-公开
标题翻译： QTLS与鉴定大豆抗宿主病的方法
公开(公告)号：WO2017139544A1
公开(公告)日：2017-08-17
申请号：PCT/US2017/017326
申请日：2017-02-10
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ATWOOD, Sarah , CRULL, Laura Jean , KUHLMAN, Leslie Charles , KYLE, Donald Earl , THOMAS, Daniel Lewis , WOODWARD, John Bryan , YANG, Ming
IPC分类号： A01H1/04 , C07K14/415 , C12Q1/68
CPC分类号： A01H1/04 , C07K14/415 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The disclosure relates to methods and compositions for identifying and/or selecting soybean plants that have resistance to lodging, have improved resistance to lodging, or are susceptible to lodging. The methods use molecular genetic markers to identify, select and/or construct resistant plants or identify and counter-select susceptible plants. Also provided are soybean plants that display resistance or improved resistance to lodging that are generated by the methods described herein. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
摘要翻译：本公开涉及用于鉴定和/或选择抗倒伏，抗倒伏性提高或易于倒伏的大豆植物的方法和组合物。该方法使用分子遗传标记来鉴定，选择和/或构建抗性植物或鉴定和反选择易感植物。还提供了显示出由本文所述方法产生的抗性或抗倒伏性的大豆植物。该摘要旨在作为用于在特定技术中进行搜索的目的的扫描工具，并且不旨在限制本公开。

4. 发明申请

WO2017079026A1 GENERATION OF COMPLEX TRAIT LOCI IN SOYBEAN AND METHODS OF USE 审中-公开
标题翻译：大豆复合型特征基因的产生及其利用方法
公开(公告)号：WO2017079026A1
公开(公告)日：2017-05-11
申请号：PCT/US2016/059093
申请日：2016-10-27
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CHAKY, Julian Marco , CIGAN, Andrew Mark , KINNEY, Anthony J. , LIU, Zhan-Bin , WOODWARD, John Bryan , YOUNG, Joshua K.
IPC分类号： C12N15/82
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided producing a complex trait locus in a genomic window of a soybean plant comprising (i) at least one transgenic target site for site specific integration integrated in at least double-strand-break target site, (ii) at least one double-strand-break target site and at least one transgene, (iii) at least one altered double-strand-break target site or (iv) any one combination of (i)-(iii). The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.
摘要翻译：提供了在大豆植物的基因组窗口中产生复合性状基因座的组合物和方法，其包含（i）至少一个用于位点特异性整合的转基因靶位点，其整合于至少双链断裂靶标（ii）至少一个双链断裂靶位点和至少一个转基因，（iii）至少一个改变的双链断裂靶位点或（iv）（i） - （iii）的任何一种组合，。双链断裂靶位点可以是但不限于锌指内切核酸酶，工程内切核酸酶，大范围核酸酶，TALEN和/或Cas核酸内切酶的靶位点。所述植物的基因组窗口可以包含至少一个感兴趣的基因组基因座，例如性状盒，转基因，突变的基因，天然基因，编辑的基因或位点特异性整合（SSI）靶位点。

5. 发明申请

WO2017139544A9 QTLS ASSOCIATED WITH AND METHODS FOR IDENTIFYING LODGING RESISTANCE IN SOYBEAN 审中-公开
公开(公告)号：WO2017139544A9
公开(公告)日：2017-08-17
申请号：PCT/US2017/017326
申请日：2017-02-10
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ATWOOD, Sarah , CRULL, Laura Jean , KUHLMAN, Leslie Charles , KYLE, Donald Earl , THOMAS, Daniel Lewis , WOODWARD, John Bryan , YANG, Ming
IPC分类号： A01H1/04 , C07K14/415 , C12Q1/68
摘要： The disclosure relates to methods and compositions for identifying and/or selecting soybean plants that have resistance to lodging, have improved resistance to lodging, or are susceptible to lodging. The methods use molecular genetic markers to identify, select and/or construct resistant plants or identify and counter-select susceptible plants. Also provided are soybean plants that display resistance or improved resistance to lodging that are generated by the methods described herein. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

6. 发明申请

WO2015179004A2 COMPOSITIONS ASSOCIATED WITH SOYBEAN REPRODUCTIVE GROWTH AND METHODS OF USE 审中-公开
标题翻译：与大豆生殖生长和使用方法相关的组合物
公开(公告)号：WO2015179004A2
公开(公告)日：2015-11-26
申请号：PCT/US2015/019917
申请日：2015-03-11
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： HANSON, Sara Elizabeth , KUHLMAN, Leslie Charles , KYLE, Donald , RIES, Landon Linn , SPEAR, Jordan , WOODWARD, John Bryan
IPC分类号： C12Q1/68
CPC分类号： A01H1/04 , A01H1/02 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158
摘要： Molecular markers associated with soybean reproductive stage, methods of their use, and compositions having one or more marker loci are provided. Methods comprise detecting at least one marker locus, detecting a haplotype, and/or detecting a marker profile. Methods may further comprise crossing a selected soybean plant with a second soybean plant. Isolated polynucleotides, primers, probes, kits, systems, etc. , are also provided, as well as plants and seeds comprising one or more marker loci and having a preferred reproductive growth phenotype.
摘要翻译：提供了与大豆繁殖阶段相关的分子标记，其使用方法和具有一个或多个标记基因座的组合物。方法包括检测至少一个标记基因座，检测单倍型和/或检测标记物谱。方法还可以包括使选定的大豆植物与第二大豆植物杂交。还提供分离的多核苷酸，引物，探针，试剂盒，系统等，以及包含一个或多个标记基因座并具有优选的生殖生长表型的植物和种子。

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