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1. 发明申请

WO2015026887A1 A SOYBEAN U6 POLYMERASE III PROMOTER AND METHODS OF USE 审中-公开
标题翻译：大豆U6聚合酶III促进剂及其使用方法
公开(公告)号：WO2015026887A1
公开(公告)日：2015-02-26
申请号：PCT/US2014/051782
申请日：2014-08-20
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew, Mark , FALCO, Saverio, Carl , LI, Zhongsen , LIU, Zhan-Bin , YOUNG, Joshua, K.
IPC分类号： C12N15/82 , C12N15/8213 , C07K14/415
CPC分类号： C12N15/8216 , C12N15/113 , C12N15/81 , C12N15/8213 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/3231 , C12N2310/3341 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12N2310/321 , C12N2310/3521 , C12N2310/322 , C12N2310/3533
摘要： The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
摘要翻译：本公开涉及来自大豆，特别是大豆U6聚合酶III基因的启动子及其片段的基因表达调控序列及其在促进植物中一种或多种异源核酸片段的表达的用途。本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，突变植物，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

2. 发明申请

WO2017079026A1 GENERATION OF COMPLEX TRAIT LOCI IN SOYBEAN AND METHODS OF USE 审中-公开
标题翻译：大豆复合型特征基因的产生及其利用方法
公开(公告)号：WO2017079026A1
公开(公告)日：2017-05-11
申请号：PCT/US2016/059093
申请日：2016-10-27
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CHAKY, Julian Marco , CIGAN, Andrew Mark , KINNEY, Anthony J. , LIU, Zhan-Bin , WOODWARD, John Bryan , YOUNG, Joshua K.
IPC分类号： C12N15/82
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided producing a complex trait locus in a genomic window of a soybean plant comprising (i) at least one transgenic target site for site specific integration integrated in at least double-strand-break target site, (ii) at least one double-strand-break target site and at least one transgene, (iii) at least one altered double-strand-break target site or (iv) any one combination of (i)-(iii). The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.
摘要翻译：提供了在大豆植物的基因组窗口中产生复合性状基因座的组合物和方法，其包含（i）至少一个用于位点特异性整合的转基因靶位点，其整合于至少双链断裂靶标（ii）至少一个双链断裂靶位点和至少一个转基因，（iii）至少一个改变的双链断裂靶位点或（iv）（i） - （iii）的任何一种组合，。双链断裂靶位点可以是但不限于锌指内切核酸酶，工程内切核酸酶，大范围核酸酶，TALEN和/或Cas核酸内切酶的靶位点。所述植物的基因组窗口可以包含至少一个感兴趣的基因组基因座，例如性状盒，转基因，突变的基因，天然基因，编辑的基因或位点特异性整合（SSI）靶位点。

3. 发明申请

WO2015026883A1 PLANT GENOME MODIFICATION USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS AND METHODS OF USE 审中-公开
标题翻译：使用指南RNA / CAS内切酶系统进行植物基因组修饰及其使用方法
公开(公告)号：WO2015026883A1
公开(公告)日：2015-02-26
申请号：PCT/US2014/051778
申请日：2014-08-20
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew , FALCO, Saverio, Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, L., Aleksander , SHI, Jinrui , SVITASHEV, Sergei , YOUNG, Joshua, K.
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/113 , C12N15/81 , C12N15/8213 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/3231 , C12N2310/3341 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12N2310/321 , C12N2310/3521 , C12N2310/322 , C12N2310/3533
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
摘要翻译：提供组合物和方法用于基因组修饰植物或植物细胞基因组中的靶序列。方法和组合物采用指导性RNA / Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统。还提供了使用引导多核苷酸/ Cas内切核酸酶系统用于细胞或生物体基因组中的核苷酸序列进行基因组修饰，用于基因编辑和/或用于在基因组中插入或缺失目标多核苷酸或从基因组中插入或缺失目的多核苷酸的组合物和方法的细胞或生物体。一旦识别出基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。还公开了利用双组分RNA引物和Cas内切核酸酶系统选择植物的育种方法和方法。还提供组合物和方法用于编辑细胞基因组中的核苷酸序列。

4. 发明申请

WO2013166113A1 COMPOSITIONS AND METHODS COMPRISING SEQUENCES HAVING MEGANUCLEASE ACTIVITY 审中-公开
标题翻译：组合物和包含游离基活性的序列的方法
公开(公告)号：WO2013166113A1
公开(公告)日：2013-11-07
申请号：PCT/US2013/039011
申请日：2013-05-01
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： BERMUDEZ, Ericka , CIGAN, Andrew, Mark , ENGLISH, James , FALCO, Saverio, Carl , GAO, Huirong , LIU, Lu , LIU, Zhan-Bin , ONG, Azalea , SVITASHEV, Sergei , YOUNG, Joshua, K.
IPC分类号： C12N9/22 , C12N15/82
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
摘要翻译：提供了包含具有大范围核酸酶活性的多核苷酸和多肽的组合物和方法。还提供了具有大范围核酸酶序列的核酸构建体，酵母，植物，植物细胞，外植体，种子和颗粒。提供了使用大范围核酸酶序列的各种方法。这样的方法包括例如用于生产在宽温度范围内具有增加的活性的大范围核酸酶的方法，用于生产酵母，植物，植物细胞，外植体或包含具有增加的活性的大范围核酸酶的种子的方法。

5. 发明申请

WO2016040030A1 GENERATION OF SITE-SPECIFIC-INTEGRATION SITES FOR COMPLEX TRAIT LOCI IN CORN AND SOYBEAN, AND METHODS OF USE 审中-公开
标题翻译：玉米和大豆中复合物种位点特异性整合位点的生成及其使用方法
公开(公告)号：WO2016040030A1
公开(公告)日：2016-03-17
申请号：PCT/US2015/047706
申请日：2015-08-31
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew Mark , GAO, Huirong , LIU, Zhan-Bin , MUTTI, Jasdeep , PODLICH, Dean , SCELONGE, Christopher
IPC分类号： C12N15/82 , A01H5/10
CPC分类号： C12N15/8213 , C12N15/8201 , C12N15/8241
摘要： Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.
摘要翻译：提供组合物和方法用于将位点特异性整合（SSI）和/或目标多核苷酸的转基因靶位点引入植物基因组的基因组窗口中双链断裂诱导剂的至少一个双链断裂靶位点。还提供了用于在植物的基因组窗口中产生复合特征基因座的方法和组合物，其包含至少一个整合在至少双链断裂靶位点中的位点特异性整合的转基因靶位点。双链断裂靶位点可以是但不限于锌指内切核酸酶，工程内切核酸酶，大范围核酸酶，TALENs和/或Cas内切核酸酶的靶位点。所述植物的基因组窗可以包含至少一个感兴趣的基因座，例如性状盒，转基因，突变基因，天然基因，编辑基因或位点特异性整合（SSI）靶位点。

6. 发明申请

WO2016007347A1 COMPOSITIONS AND METHODS FOR PRODUCING PLANTS RESISTANT TO GLYPHOSATE HERBICIDE 审中-公开
标题翻译：用于生产耐草甘膦杀虫剂的植物的组合物和方法
公开(公告)号：WO2016007347A1
公开(公告)日：2016-01-14
申请号：PCT/US2015/038767
申请日：2015-07-01
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： DJUKANOVIC, Vesna , JONES, Spencer Charles , LIU, Zhan-Bin , LASSNER, Michael , LYZNIK, L. Aleksander
IPC分类号： A01H1/02 , C12N15/113 , C12N15/82
CPC分类号： C12N15/8275 , A01H1/02 , A01H4/008 , C12N15/8213 , C12N15/8241 , C12N2310/20 , C12Y301/00
摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
摘要翻译：本公开内容包括产生对膦酰基甲基甘氨酸家族除草剂具有抗性的突变植物，例如，草甘膦。提供组合物和方法用于使用引导多核苷酸/ Cas内切核酸酶系统编码细胞中的目的核苷酸序列，其中所述Cas内切核酸酶由引导多核苷酸引导以识别并任选地将特定靶位点处的双链断裂引入到细胞的基因组。要编辑的感兴趣的核苷酸序列可以位于由Cas内切核酸酶识别的靶位点内部或外部。更具体地，提供组合物和方法用于在细胞中编辑烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）核苷酸序列。方法和组合物采用引导多核苷酸/ Cas内切核酸酶系统来提供用于编辑细胞基因组内的EPSPS核苷酸序列的有效系统。还提供了用于生产耐受草甘膦植物细胞，植物外植体，种子和谷粒的组合物和方法。

7. 发明申请

WO2015026886A1 METHODS FOR PRODUCING GENETIC MODIFICATIONS IN A PLANT GENOME WITHOUT INCORPORATING A SELECTABLE TRANSGENE MARKER, AND COMPOSITIONS THEREOF 审中-公开
标题翻译：在不配合选择性转基因标记的情况下在植物基因组中生产遗传修饰的方法及其组合物
公开(公告)号：WO2015026886A1
公开(公告)日：2015-02-26
申请号：PCT/US2014/051781
申请日：2014-08-20
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew, Mark , FALCO, Saverio, Carl , LASSNER, Michael , LIU, Zhan-Bin , SVITASHEV, Sergei
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/113 , C12N15/81 , C12N15/8213 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/3231 , C12N2310/3341 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12N2310/321 , C12N2310/3521 , C12N2310/322 , C12N2310/3533
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide / Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide / Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.
摘要翻译：提供组合物和方法用于在植物或植物细胞的基因组中靶基因组的基因组修饰，而不加入可选择的转基因标记。方法和组合物采用引导多核苷酸/ Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统，而不需要并入可选择的转基因标记。一旦识别出基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。还公开了利用引导多核苷酸/ Cas内切核酸酶系统选择植物的育种方法和方法。还提供组合物和方法用于编辑细胞基因组中的核苷酸序列，而不包含可选择的转基因标记。

8. 发明公开

EP3166391A1 COMPOSITIONS AND METHODS FOR PRODUCING PLANTS RESISTANT TO GLYPHOSATE HERBICIDE 审中-公开
标题翻译：用于制造抗耐药性植物的组合物和方法草甘膦除草剂
公开(公告)号：EP3166391A1
公开(公告)日：2017-05-17
申请号：EP15739713.4
申请日：2015-07-01
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： DJUKANOVIC, Vesna , JONES, Spencer Charles , LIU, Zhan-Bin , LASSNER, Michael , LYZNIK, Leszek, Aleksander
IPC分类号： A01H1/02 , C12N15/113 , C12N15/82
CPC分类号： C12N15/8275 , A01H1/02 , A01H4/008 , C12N15/8213 , C12N15/8241 , C12N2310/20 , C12Y301/00
摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
摘要翻译：本公开包括在phosphonomethylglycines家族的除草剂的生产突变植物抗性，例如草甘膦。提供了用于在细胞中编辑感兴趣的核苷酸序列采用导向的多核苷酸/ CAS核酸内切酶系统，worin中科院内切核酸酶通过导向的多核苷酸导入到识别和任选的特定靶位点到引入双海滩断组合物和方法细胞的基因组中。要被编辑的感兴趣的核苷酸序列可以位于内或在靶位点外也通过将CAS核酸内切酶识别。更具体地，组合物和方法提供了用于在细胞烯醇丙酮莽草酸-3-磷酸合酶（EPSPS）的核苷酸序列的编辑。所述方法和组合物使用的多核苷酸导/ CAS核酸内切酶在系统有效的系统，以提供用于编辑EPSPS细胞内的基因组中的核苷酸序列。因此，提供了组合物和方法用于生产草甘膦耐性植物细胞，植物外植体，种子和谷物。

9. 发明公开

EP3628739A1 GENERATION OF SITE-SPECIFIC-INTEGRATION SITES FOR COMPLEX TRAIT LOCI IN CORN AND SOYBEAN, AND METHODS OF USE 有权转让
公开(公告)号：EP3628739A1
公开(公告)日：2020-04-01
申请号：EP19206649.6
申请日：2015-08-31
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew Mark , GAO, Huirong , LIU, Zhan-Bin , MUTTI, Jasdeep , PODLICH, Dean , SCELONGE, Christopher
IPC分类号： C12N15/82 , A01H5/10
摘要： Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.

10. 发明公开

EP3036334A1 A SOYBEAN U6 POLYMERASE III PROMOTER AND METHODS OF USE 审中-公开
标题翻译：索非亚N6-POLYMERASE-III-PROMOTOR UND VERFAHREN ZUR VERWENDUNG
公开(公告)号：EP3036334A1
公开(公告)日：2016-06-29
申请号：EP14761479.6
申请日：2014-08-20
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew Mark , FALCO, Saverio Carl , LI, Zhongsen , LIU, Zhan-Bin , YOUNG, Joshua K.
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8216 , C12N15/113 , C12N15/81 , C12N15/8213 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/3231 , C12N2310/3341 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12N2310/321 , C12N2310/3521 , C12N2310/322 , C12N2310/3533
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
摘要翻译：提供组合物和方法用于植物或植物细胞基因组中靶序列的基因组修饰。方法和组合物采用指导性RNA / Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统。还提供了使用引导多核苷酸/ Cas内切核酸酶系统用于细胞或生物体基因组中的核苷酸序列进行基因组修饰，用于基因编辑和/或用于在基因组中插入或缺失目标多核苷酸或从基因组中插入或缺失目的多核苷酸的组合物和方法的细胞或生物体。一旦确定了基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。还公开了利用双组分RNA引物和Cas内切核酸酶系统选择植物的育种方法和方法。还提供组合物和方法用于编辑细胞基因组中的核苷酸序列。

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