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    • 2. 发明申请
    • FAMILY OF MAIZE PR-1 GENES AND PROMOTERS
    • 玉米PR-1基因家族和启动子
    • WO9943819B1
    • 1999-10-14
    • PCT/US9903011
    • 1999-02-11
    • PIONEER HI BRED INTCRANE VIRGINIA C
    • CRANE VIRGINIA C
    • C07K14/415C12N15/29C12N15/82A01H5/00A01H5/10C12N5/10
    • C12N15/8216C07K14/415C12N15/8238C12N15/8239C12N15/8279
    • The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for inducible and constitutive promoters isolated from a family of maize genes encoding pathogenesis-related (PR-1) proteins, nucleotide sequences for these maize PR-1 genes, and amino acid sequences for the PR-1 proteins encoded thereby. Methods for regulating expression of a heterologous nucleotide sequence in a plant in an inducible or constitutive manner are provided, more particularly regulating expression of a PR-1 gene to enhance disease resistance in a plant. The methods comprise transforming a plant cell to comprise a heterologous nucleotide sequence or PR-1 gene operably linked to one of the inducible or constitutive promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell. Transformed plants and seeds having enhanced disease resistance are also provided.
    • 本发明提供了用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物是从编码致病相关(PR-1)蛋白的玉米基因家族,这些玉米PR-1基因的核苷酸序列和由其编码的PR-1蛋白的氨基酸序列分离的诱导型和组成型启动子的新型核苷酸序列 。 提供了以诱导型或组成型方式调节植物中异源核苷酸序列表达的方法,更特别是调节PR-1基因的表达以增强植物的抗病性。 所述方法包括转化植物细胞以包含与本发明的诱导型或组成型启动子之一可操作连接的异源核苷酸序列或PR-1基因,并从转化的植物细胞再生稳定转化的植物。 还提供了具有增强的抗病性的转化的植物和种子。