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1. 发明申请

WO2009099481A1 MAIZE LEAF- AND STALK-PREFERRED PROMOTER 审中-公开
标题翻译： MAIZE LEAF-和STALK优先促销
公开(公告)号：WO2009099481A1
公开(公告)日：2009-08-13
申请号：PCT/US2008/086539
申请日：2008-12-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CRANE, Virginia C. , SANDAHL, Jeanne
发明人： CRANE, Virginia C. , SANDAHL, Jeanne
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8226
摘要： The present disclosure provides compositions and methods for regulating 5 expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably 10 linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了调节植物中异源核苷酸序列的5种表达的组合物和方法。组合物包括用于组织优选的玉米启动子的新的核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的组织优选启动子连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

2. 发明申请

WO2009026580A3 MAIZE PROMOTER ACTIVE IN SILK AND PERICARP TISSUES 审中-公开
标题翻译：玉米促销活跃丝绸和PERICARP组织
公开(公告)号：WO2009026580A3
公开(公告)日：2009-06-18
申请号：PCT/US2008074177
申请日：2008-08-25
申请人： PIONEER HI BRED INT , CRANE VIRGINIA C , SANDAHL JEANNE , SIMMONS CARL R
发明人： CRANE VIRGINIA C , SANDAHL JEANNE , SIMMONS CARL R
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8233 , C12N15/8234 , C12N15/8235
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括组织优选玉米启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的组织优选型启动子有效连接的核苷酸序列稳定地并入植物细胞的基因组，并再生表达该核苷酸序列的稳定转化的植物。

3. 发明申请

WO2007149657A2 ANTIFUNGAL POLYPEPTIDES 审中-公开
标题翻译：抗菌多糖
公开(公告)号：WO2007149657A2
公开(公告)日：2007-12-27
申请号：PCT/US2007068984
申请日：2007-05-15
申请人： PIONEER HI BRED INT , DU PONT , UNIV CALIFORNIA , ALTIER DANIEL J , CRANE VIRGINIA C , GILLIAM JACOB T , HUNTER-CEVERA JENNIE , PRESNAIL JAMES K , SCHEPERS ERIC J , SIMMONS CARL R , TOROK TAMAS , YALPANI NASSER
发明人： ALTIER DANIEL J , CRANE VIRGINIA C , GILLIAM JACOB T , HUNTER-CEVERA JENNIE , PRESNAIL JAMES K , SCHEPERS ERIC J , SIMMONS CARL R , TOROK TAMAS , YALPANI NASSER , ELLANSKAYA I A
CPC分类号： C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。也提供了编码抗病原多肽的核酸。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。

4. 发明申请

WO2007050509A1 MAIZE PROMOTER ACTIVE IN SILKS, STALK NODES, ROOTS AND LEAF SHEATHS 审中-公开
标题翻译：绢丝，K ES ES，HS HS HS HS HS HS HS HS HS HS HS HS HS
公开(公告)号：WO2007050509A1
公开(公告)日：2007-05-03
申请号：PCT/US2006/041277
申请日：2006-10-23
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CRANE, Virginia C. , RICE, Douglas A. , SANDAHL, Jeanne M.
发明人： CRANE, Virginia C. , RICE, Douglas A. , SANDAHL, Jeanne M.
IPC分类号： C12N15/29 , C12N15/82 , C12N5/04 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8222 , C07K14/415
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于组织优选的玉米启动子的新的核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

5. 发明申请

WO2014116854A1 MAIZE EVENT DP-033121-3 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-033121-3及其检测方法
公开(公告)号：WO2014116854A1
公开(公告)日：2014-07-31
申请号：PCT/US2014/012787
申请日：2014-01-23
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , BEATTY, Mary , BRINK, Kent , CRANE, Virginia C. , DIEHN, Scott , LU, Albert L. , YOUNG, Gregory J.
发明人： BEATTY, Mary , BRINK, Kent , CRANE, Virginia C. , DIEHN, Scott , LU, Albert L. , YOUNG, Gregory J.
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8286 , A01H5/10 , C07K14/325 , C12N15/8277 , C12Q1/6895 , C12Q2600/13 , Y02A40/162
摘要： The disclosure provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-033121-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本公开提供与转基因抗虫玉米植物相关的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-033121-3事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

6. 发明申请

WO2009026580A2 MAIZE PROMOTER ACTIVE IN SILK AND PERICARP TISSUES 审中-公开
标题翻译：在SILK和PERICARP组织中活化的促进剂
公开(公告)号：WO2009026580A2
公开(公告)日：2009-02-26
申请号：PCT/US2008/074177
申请日：2008-08-25
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CRANE, Virginia C. , SANDAHL, Jeanne , SIMMONS, Carl R.
发明人： CRANE, Virginia C. , SANDAHL, Jeanne , SIMMONS, Carl R.
CPC分类号： C12N15/8233 , C12N15/8234 , C12N15/8235
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于组织优选的玉米启动子的新的核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的组织优选启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

7. 发明申请

WO9943819B1 FAMILY OF MAIZE PR-1 GENES AND PROMOTERS 审中-公开
标题翻译：玉米PR-1基因家族和启动子
公开(公告)号：WO9943819B1
公开(公告)日：1999-10-14
申请号：PCT/US9903011
申请日：1999-02-11
申请人： PIONEER HI BRED INT , CRANE VIRGINIA C
发明人： CRANE VIRGINIA C
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , A01H5/00 , A01H5/10 , C12N5/10
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8238 , C12N15/8239 , C12N15/8279
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for inducible and constitutive promoters isolated from a family of maize genes encoding pathogenesis-related (PR-1) proteins, nucleotide sequences for these maize PR-1 genes, and amino acid sequences for the PR-1 proteins encoded thereby. Methods for regulating expression of a heterologous nucleotide sequence in a plant in an inducible or constitutive manner are provided, more particularly regulating expression of a PR-1 gene to enhance disease resistance in a plant. The methods comprise transforming a plant cell to comprise a heterologous nucleotide sequence or PR-1 gene operably linked to one of the inducible or constitutive promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell. Transformed plants and seeds having enhanced disease resistance are also provided.
摘要翻译：本发明提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物是从编码致病相关（PR-1）蛋白的玉米基因家族，这些玉米PR-1基因的核苷酸序列和由其编码的PR-1蛋白的氨基酸序列分离的诱导型和组成型启动子的新型核苷酸序列。提供了以诱导型或组成型方式调节植物中异源核苷酸序列表达的方法，更特别是调节PR-1基因的表达以增强植物的抗病性。所述方法包括转化植物细胞以包含与本发明的诱导型或组成型启动子之一可操作连接的异源核苷酸序列或PR-1基因，并从转化的植物细胞再生稳定转化的植物。还提供了具有增强的抗病性的转化的植物和种子。

8. 发明申请

WO2014116989A1 MAIZE EVENT DP-032218-9 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-032218-9及其检测方法
公开(公告)号：WO2014116989A1
公开(公告)日：2014-07-31
申请号：PCT/US2014/013007
申请日：2014-01-24
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , BEATTY, Mary E. H. , BRINK, Kent , CRANE, Virginia C. , DIEHN, Scott , LU, Albert L. , YOUNG, Gregory J.
发明人： BEATTY, Mary E. H. , BRINK, Kent , CRANE, Virginia C. , DIEHN, Scott , LU, Albert L. , YOUNG, Gregory J.
IPC分类号： C12N15/82
CPC分类号： C12Q1/6895 , A01H5/10 , C12N15/8277 , C12N15/8286 , C12Q2600/13 , C12Q2600/158 , Y02A40/162
摘要： The disclosure provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-032218-9 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本公开提供与转基因抗虫玉米植物相关的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-032218-9事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

9. 发明申请

WO2012030759A1 VACUOLE TARGETING PEPTIDES AND METHODS OF USE 审中-公开
标题翻译： VACUOLE目标肽和使用方法
公开(公告)号：WO2012030759A1
公开(公告)日：2012-03-08
申请号：PCT/US2011/049639
申请日：2011-08-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CRANE, Virginia C. , YALPANI, Nasser , SANDAHL, Jeanne
发明人： CRANE, Virginia C. , YALPANI, Nasser , SANDAHL, Jeanne
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C07K14/415 , A01N37/46 , A01N65/00 , C12N15/8221
摘要： Compositions and methods for targeting polypeptides to plant vacuoles are provided. Nucleic acid molecules having nucleotide sequences encoding a vacuole-targeting peptide, variants, or fragments thereof are provided. The sequences also can be used for targeting defensin proteins or other polypeptides to vacuoles in plants. Transformed plants, plant cells, tissues, and seed also are provided.
摘要翻译：提供了将多肽靶向植物空泡的组合物和方法。提供了具有编码液泡靶向肽，变体或其片段的核苷酸序列的核酸分子。序列也可用于将防御素蛋白或其他多肽靶向植物中的空泡。还提供了转化的植物，植物细胞，组织和种子。

10. 发明申请

WO2007149657A3 ANTIFUNGAL POLYPEPTIDES 审中-公开
公开(公告)号：WO2007149657A3
公开(公告)日：2007-12-27
申请号：PCT/US2007/068984
申请日：2007-05-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , ALTIER, Daniel, J. , CRANE, Virginia, C. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
发明人： ALTIER, Daniel, J. , CRANE, Virginia, C. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
IPC分类号： C12N15/82 , C07K14/385
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

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