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1. 发明申请

WO2018100370A1 METHODS AND SYSTEMS FOR CHARACTERIZING ANALYTES USING NANOPORES 审中-公开
公开(公告)号：WO2018100370A1
公开(公告)日：2018-06-07
申请号：PCT/GB2017/053603
申请日：2017-11-29
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： CLARKE, James , WHITE, James , MUSCAT, Richard , KNOT, Jessica Mary May , NATHANI, Ramiz Iqbal , HERON, Andrew John , BRUCE, Mark John , JAYASINGHE, Lakmal Nishantha , CAPROTTI, Domenico , STODDART, David Jackson , BOWEN, Rebecca Victoria , WRIGHT, Christopher James , MOODY, Paul Richard
IPC分类号： C12Q1/6869
摘要： Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g ., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g ., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

2. 发明申请

WO2015140535A1 ANALYSIS OF A POLYMER FROM MULTI-DIMENSIONAL MEASUREMENTS 审中-公开
标题翻译：聚合物从多尺度测量中的分析
公开(公告)号：WO2015140535A1
公开(公告)日：2015-09-24
申请号：PCT/GB2015/050776
申请日：2015-03-17
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： DOLAN, Kevin Thomas , WRIGHT, Christopher James
IPC分类号： G06F19/22 , G01N33/487
CPC分类号： C12Q1/6869 , G01N33/48721 , G06F19/22 , G06F19/24 , C12Q2537/165 , C12Q2565/631
摘要： A target sequence of polymer units is estimated from plural series of measurements taken from sequences of polymer units that comprise the target sequence or a complementary sequence. Each measurement is dependent on a k-mer (k polymer units). Models treat the measurements as observations of k-mer states, comprising transition weightings in respect of transitions between successive k-mer states and emission weightings for different measurements being observed. An estimated alignment mapping between the plural series of measurements is derived based on an application of the models to each series. An estimate of the target sequence of polymer units is generated by applying the models, treating the types of k-mer state of each model and the measurements as dimensions of a plural dimensional k-mer state and plural dimensional observations. Constraint of paths through the plural dimensional k-mer states using the derived alignment mapping greatly reduces the required processing.
摘要翻译：通过从包含靶序列或互补序列的聚合物单元序列获得的多个系列测量来估计聚合物单元的靶序列。每个测量取决于k-mer（k聚合物单位）。模型将测量值视为k-mer状态的观测值，其包括关于连续k-mer状态之间的跃迁的转换权重和针对观察到的不同测量的发射权重。基于对每个系列的模型的应用，导出多个测量系列之间的估计的对准映射。通过应用模型，处理每个模型的k-mer状态的类型和测量作为多维k-mer状态和多维观察的维度来生成聚合物单元的靶序列的估计。使用导出的对准映射通过多维k-mer状态的路径的约束大大减少了所需的处理。

3. 发明申请

WO2019234432A1 METHOD 审中-公开
公开(公告)号：WO2019234432A1
公开(公告)日：2019-12-12
申请号：PCT/GB2019/051571
申请日：2019-06-06
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： CLARKE, James Anthony , WHITE, James , MUSCAT, Richard , JOHNSON, Jessica Mary May , NATHANI, Ramiz Iqbal , HERON, Andrew John , BRUCE, Mark John , JAYASINGHE, Lakmal Nishantha , CAPROTTI, Domenico , STODDART, David Jackson , BOWEN, Rebecca Victoria , WRIGHT, Christopher James , MOODY, Paul Richard
IPC分类号： C12Q1/6869
摘要： Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

4. 发明申请

WO2016059427A1 ANALYSIS OF A POLYMER 审中-公开
标题翻译：聚合物分析
公开(公告)号：WO2016059427A1
公开(公告)日：2016-04-21
申请号：PCT/GB2015/053083
申请日：2015-10-16
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： REID, Stuart William , HARPER, Gavin , BROWN, Clive Gavin , TURNER, Daniel John , HERON, Andrew John , WRIGHT, Christopher James
IPC分类号： C12Q1/68 , G01N33/487 , G06F19/22
CPC分类号： C12Q1/6869 , G01N33/48721 , G06F19/22 , C12Q2565/631
摘要： Analysis Of A Polymer A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.
摘要翻译：聚合物的分析生化分析系统通过在聚合物穿过纳米孔的位移期间从包含纳米孔的传感器元件中测量聚合物来分析聚合物。当聚合物部分移位时，使用从参考序列得到的参考数据来分析一系列测量结果，以提供相似性的度量。响应于相似性的测量，可以选择性地操作传感器元件以喷射聚合物，从而使纳米孔可用于接收另外的聚合物。当生物化学分析系统包括传感器元件阵列并且以多路复用方式选择的传感器元件进行测量时，响应于相似性的测量，生物化学分析系统停止从当前选择的传感器元件进行测量并开始从新选择的传感器元件。

5. 发明公开

EP3207154A1 ANALYSIS OF A POLYMER 有权转让
标题翻译：一种聚合物的分析
公开(公告)号：EP3207154A1
公开(公告)日：2017-08-23
申请号：EP15784133.9
申请日：2015-10-16
申请人： Oxford Nanopore Technologies Limited
发明人： REID, Stuart William , HARPER, Gavin , BROWN, Clive Gavin , TURNER, Daniel John , HERON, Andrew John , WRIGHT, Christopher James
IPC分类号： C12Q1/68 , G01N33/487 , G06F19/22
CPC分类号： C12Q1/6869 , G01N33/48721 , G06F19/22 , C12Q2565/631
摘要： Analysis Of A Polymer A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.
摘要翻译：聚合物的分析生物化学分析系统通过在聚合物穿过纳米孔移位过程中测量来自包含纳米孔的传感器元件的聚合物来分析聚合物。当聚合物部分易位时，使用来自参考序列的参考数据分析一系列测量结果以提供相似性度量。响应于相似性的测量，可以选择性地操作传感器元件以喷射聚合物并由此使得纳米孔可用于接收另外的聚合物。在生物化学分析系统包括传感器元件阵列并且从多路复用方式中选择的传感器元件进行测量的情况下，响应于相似性度量，生化分析系统停止从当前选择的传感器元件进行测量并且开始从新选择的传感器元件。

7. 发明公开

EP3120277A1 ANALYSIS OF A POLYMER FROM MULTI-DIMENSIONAL MEASUREMENTS 审中-公开
标题翻译： ANALYZE EINES POLYMERS AUS MEHRDIMENSIONALEN MESSUNGEN
公开(公告)号：EP3120277A1
公开(公告)日：2017-01-25
申请号：EP15713553.4
申请日：2015-03-17
申请人： Oxford Nanopore Technologies Limited
发明人： DOLAN, Kevin Thomas , WRIGHT, Christopher James
IPC分类号： G06F19/22 , G01N33/487
CPC分类号： C12Q1/6869 , G01N33/48721 , G06F19/22 , G06F19/24 , C12Q2537/165 , C12Q2565/631
摘要： A target sequence of polymer units is estimated from plural series of measurements taken from sequences of polymer units that comprise the target sequence or a complementary sequence. Each measurement is dependent on a k-mer (k polymer units). Models treat the measurements as observations of k-mer states, comprising transition weightings in respect of transitions between successive k-mer states and emission weightings for different measurements being observed. An estimated alignment mapping between the plural series of measurements is derived based on an application of the models to each series. An estimate of the target sequence of polymer units is generated by applying the models, treating the types of k-mer state of each model and the measurements as dimensions of a plural dimensional k-mer state and plural dimensional observations. Constraint of paths through the plural dimensional k-mer states using the derived alignment mapping greatly reduces the required processing.
摘要翻译：通过从包含靶序列或互补序列的聚合物单元的序列获得的多个系列测量来估计聚合物单元的靶序列。每个测量取决于k-mer（k聚合物单位）。模型将测量值视为k-mer状态的观测值，其包括关于连续k-mer状态之间的转变的转移权重和针对观察到的不同测量的发射权重。基于对每个系列的模型的应用，导出多个测量系列之间的估计的对准映射。聚合物单元的目标序列的估计是通过应用模型，将每个模型的k-mer状态的类型和测量值作为多维k-mer状态的维度和多维观测值来生成的。使用导出的对准映射通过多维k-mer状态的路径的约束大大减少了所需的处理。

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