专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2018100370A1 METHODS AND SYSTEMS FOR CHARACTERIZING ANALYTES USING NANOPORES 审中-公开
公开(公告)号：WO2018100370A1
公开(公告)日：2018-06-07
申请号：PCT/GB2017/053603
申请日：2017-11-29
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： CLARKE, James , WHITE, James , MUSCAT, Richard , KNOT, Jessica Mary May , NATHANI, Ramiz Iqbal , HERON, Andrew John , BRUCE, Mark John , JAYASINGHE, Lakmal Nishantha , CAPROTTI, Domenico , STODDART, David Jackson , BOWEN, Rebecca Victoria , WRIGHT, Christopher James , MOODY, Paul Richard
IPC分类号： C12Q1/6869
摘要： Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g ., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g ., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

2. 发明申请

WO2016132123A1 HETERO-PORES 审中-公开
标题翻译：异质孔隙
公开(公告)号：WO2016132123A1
公开(公告)日：2016-08-25
申请号：PCT/GB2016/050390
申请日：2016-02-17
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： BROWN, Clive Gavin , CLARKE, James Anthony , HAMBLEY, Richard George , HERON, Andrew John , JAYASINGHE, Lakmal , MILTON, John , PUGH, Jonathan Bankes , WALLACE, Elizabeth Jayne
IPC分类号： C07K14/35 , C12Q1/68
CPC分类号： C12Q1/6869 , C07K14/35
摘要： The invention relates to hetero-oligomeric pores derived from Msp. The invention also relates to polynucleotide characterisation using the hetero-oligomeric pores.
摘要翻译：本发明涉及源自Msp的异低聚物孔。本发明还涉及使用异低聚物孔的多核苷酸表征。

3. 发明申请

WO2015166275A1 MUTANT PORES 审中-公开
公开(公告)号：WO2015166275A1
公开(公告)日：2015-11-05
申请号：PCT/GB2015/051290
申请日：2015-05-01
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： CLARKE, James Anthony , HERON, Andrew John , JAYASINGHE, Lakmal , WALLACE, Elizabeth Jayne
IPC分类号： C07K14/35 , C12Q1/68
CPC分类号： C12Q1/6869 , C07K14/35 , C12Q2563/157 , C12Q2565/631
摘要： The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.
摘要翻译：本发明涉及Msp的突变形式。本发明还涉及使用Msp的多核苷酸表征。

4. 发明申请

WO2013057495A3 METHOD OF CHARACTERIZING A TARGET POLYNUCLEOTIDE USING A PORE AND A HEL308 HELICASE 审中-公开
公开(公告)号：WO2013057495A3
公开(公告)日：2013-04-25
申请号：PCT/GB2012/052579
申请日：2012-10-18
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： MOYSEY, Ruth , HERON, Andrew John
IPC分类号： C12Q1/68
摘要： The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.

5. 发明申请

WO2016055778A1 MUTANT PORES 审中-公开
公开(公告)号：WO2016055778A1
公开(公告)日：2016-04-14
申请号：PCT/GB2015/052917
申请日：2015-10-06
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： JAYASINGHE, Lakmal , WALLACE, Elizabeth Jayne , PUGH, Jonathan Bankes , HAMBLEY, Richard George , WOOD, Neil Roger , HERON, Andrew John , WHITE, James
IPC分类号： C12Q1/68 , C07K14/35
CPC分类号： C12Q1/6869 , B01L3/502761 , B01L2200/0663 , B01L2300/0636 , B01L2300/0896 , C07K14/35 , C12Q1/68 , G01N33/48721 , C12Q2565/607 , C12Q2565/631
摘要： The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.
摘要翻译：本发明涉及Msp的突变形式。本发明还涉及使用Msp的多核苷酸表征。

6. 发明申请

WO2015110777A1 METHOD FOR CONTROLLING THE MOVEMENT OF A POLYNUCLEOTIDE THROUGH A TRANSMEMBRANE PORE 审中-公开
标题翻译：通过透明质子管控制多核苷酸运动的方法
公开(公告)号：WO2015110777A1
公开(公告)日：2015-07-30
申请号：PCT/GB2014/052737
申请日：2014-09-10
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： BOWEN, Rebecca Victoria , BROWN, Clive Gavin , BRUCE, Mark , HERON, Andrew John , WALLACE, Jayne Elizabeth , WHITE, James , LLOYD, Joseph Hargreaves , ALVES, David Antoni , CAPROTTI, Domenico , JAYASINGHE, Lakmal , MCNEILL, Luke , MILTON, John , PUGLISI, Antonino , SOEROES, Szabolcs
IPC分类号： C12Q1/68 , G01N33/487
CPC分类号： C12Q1/6869 , G01N27/4145 , G01N27/44721 , G01N27/4473 , G01N27/44752 , G01N27/44791 , C12Q2521/513 , C12Q2565/631
摘要： The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterising target polynucleotides using helicases.
摘要翻译：本发明涉及通过跨膜孔控制多核苷酸运动的新方法。本发明还涉及使用解旋酶表征靶多核苷酸的新方法。

7. 发明申请

WO2014064444A1 DROPLET INTERFACES 审中-公开
标题翻译： DROPLET接口
公开(公告)号：WO2014064444A1
公开(公告)日：2014-05-01
申请号：PCT/GB2013/052767
申请日：2013-10-23
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： HERON, Andrew John , HYDE, Jason Robert , BROWN, Clive Gavin
IPC分类号： B01D69/00 , C12Q1/68 , G01N33/50
CPC分类号： G01N33/48721 , B01D67/0023 , B01D69/122 , B01D71/26 , B01D71/32 , B01D71/58 , B01D71/70 , B01D71/80 , B01D2323/35 , B01D2325/36 , B01D2325/38 , C12Q1/6869 , G01N33/50 , Y10T436/145555 , C12Q2565/631
摘要： The invention provides a method of forming a membrane between a first volume of polar medium and a second volume of polar medium, which method comprises: (a) providing a first volume comprising polar medium and a second volume comprising polar medium which are separated from one another by an apolar medium, wherein at least one of said first and second volumes comprises a layer comprising amphipathic molecules, at the interface between the polar medium and the apolar medium, wherein each of the amphipathic molecules comprises a first outer hydrophilic group, a hydrophobic core group, and a second outer hydrophilic group, wherein each of the first and second outer hydrophilic groups is linked to the hydrophobic core group; and (b) causing the first and second volumes to come into contact with one another to form a membrane comprising said amphipathic molecules between the first and second volumes. The invention also provides a system comprising a membrane between a first volume of a polar medium; and a second volume of a polar medium, which membrane comprises the amphipathic molecules, and wherein the first volume of polar medium is within an apolar medium.
摘要翻译：本发明提供了一种在第一体积的极性介质和第二体积的极性介质之间形成膜的方法，该方法包括：（a）提供包含极性介质的第一体积和包含极性介质的第二体积，所述极性介质与第一体积的极性介质分离另一个通过非极性介质，其中所述第一和第二体积中的至少一个包含在极性介质和非极性介质之间的界面处的包含两亲性分子的层，其中每个两亲性分子包含第一外部亲水基团，疏水性核心基团和第二外部亲水基团，其中第一和第二外部亲水基团中的每一个连接到疏水性核心基团; 和（b）使第一和第二体积彼此接触以形成包含第一和第二体积之间的所述两亲分子的膜。本发明还提供一种系统，其包括在第一体积的极性介质之间的膜; 和第二体积的极性介质，所述膜包含两亲性分子，并且其中所述第一体积的极性介质在非极性介质内。

8. 发明申请

WO2012107778A3 MUTANT PORES 审中-公开
公开(公告)号：WO2012107778A3
公开(公告)日：2012-08-16
申请号：PCT/GB2012/050301
申请日：2012-02-10
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED , CLARKE, James , HERON, Andrew John , JAYASINGHE, Lakmal , WALLACE, Jayne , WHITE, James
发明人： CLARKE, James , HERON, Andrew John , JAYASINGHE, Lakmal , WALLACE, Jayne , WHITE, James
IPC分类号： G01N33/487 , C12Q1/68
摘要： The invention relates to mutant forms of Msp. The invention also relates to nucleic acid characterisation using Msp.

9. 发明申请

WO2021255477A1 METHOD OF REPEATEDLY MOVING A DOUBLE-STRANDED POLYNUCLEOTIDE THROUGH A NANOPORE 审中-公开
公开(公告)号：WO2021255477A1
公开(公告)日：2021-12-23
申请号：PCT/GB2021/051557
申请日：2021-06-18
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： GRAHAM, James Edward , YOUD, Christopher Peter , BROWN, Clive Gavin , CRAWFORD, Marion Louise , HERON, Andrew John , WHITE, James
IPC分类号： C12Q1/6869
摘要： Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.

10. 发明申请

WO2021255476A2 METHOD OF CHARACTERISING A POLYNUCLEOTIDE MOVING THROUGH A NANOPORE 审中-公开
公开(公告)号：WO2021255476A2
公开(公告)日：2021-12-23
申请号：PCT/GB2021/051556
申请日：2021-06-18
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： BOWEN, Rebecca Victoria , BROWN, Clive Gavin , BRUCE, Mark John , GARALDE, Daniel Ryan , GRAHAM, James Edward , HERON, Andrew John , RAIMONDEAU, Etienne , WHITE, James , YOUD, Christopher Peter
IPC分类号： C12Q1/6869
摘要： Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式