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1. 发明申请

WO2005121351A3 METHODS FOR TRANSFORMING AND EXPRESSION SCREENING OF FILAMENTOUS FUNGAL CELLS WITH A DNA LIBRARY 审中-公开
公开(公告)号：WO2005121351A3
公开(公告)日：2005-12-22
申请号：PCT/US2005/018871
申请日：2005-05-26
申请人： NOVOZYMES, INC.
发明人： TETER, Sarah , LAMSA, Michael , CHEERY, Joel , WARD, Connie
IPC分类号： C12P21/06
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides.

2. 发明申请

WO2007115201A3 POLYPEPTIDES HAVING ENDOGLUCANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
公开(公告)号：WO2007115201A3
公开(公告)日：2007-10-11
申请号：PCT/US2007/065682
申请日：2007-03-30
申请人： NOVOZYMES, INC. , NOVOZYMES A/S , HARRIS, Paul , VLASENKO, Elena , KAUPPINNEN, Marcus Sakari , ZARETSKY, Elizabeth , TETER, Sarah , BROWN, Kimberly
发明人： HARRIS, Paul , VLASENKO, Elena , KAUPPINNEN, Marcus Sakari , ZARETSKY, Elizabeth , TETER, Sarah , BROWN, Kimberly
IPC分类号： C12N9/42 , C12N15/82 , C12N15/56 , C07K14/37
摘要： The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

3. 发明申请

WO2008151079A2 COMPOSITIONS FOR DEGRADING CELLULOSIC MATERIAL 审中-公开
标题翻译：降解纤维素材料的组合物
公开(公告)号：WO2008151079A2
公开(公告)日：2008-12-11
申请号：PCT/US2008/065417
申请日：2008-05-30
申请人： NOVOZYMES, INC. , MERINO, Sandra , MCFARLAND, Keith , CHERRY, Joel , TETER, Sarah
发明人： MERINO, Sandra , MCFARLAND, Keith , CHERRY, Joel , TETER, Sarah
IPC分类号： C12N1/00
CPC分类号： C12N9/2437 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions.
摘要翻译：本发明涉及用于降解或转化含纤维素材料的纤维素分解组合物以及制备和使用该组合物的方法。

4. 发明公开

EP1766040A2 METHODS FOR TRANSFORMING AND EXPRESSION SCREENING OF FILAMENTOUS FUNGAL CELLS WITH A DNA LIBRARY 有权
标题翻译：程序线程蘑菇细胞与DNA文库转化和表达筛选
公开(公告)号：EP1766040A2
公开(公告)日：2007-03-28
申请号：EP05786779.8
申请日：2005-05-26
申请人： Novozymes, Inc.
发明人： TETER, Sarah , LAMSA, Michael , CHEERY, Joel , WARD, Connie
IPC分类号： C12P21/06 , C12N1/16 , C12N15/74
CPC分类号： C12N15/1037 , C12N15/1086 , C12N15/80 , C40B40/02
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides.

5. 发明公开

EP1660637A2 VARIANTS OF GLYCOSIDE HYDROLASES 审中-公开
标题翻译： VARIANTEN VON GLYCOSID-HYDROLASEN
公开(公告)号：EP1660637A2
公开(公告)日：2006-05-31
申请号：EP04809617.6
申请日：2004-08-25
申请人： Novozymes Inc.
发明人： TETER, Sarah , CHERRY, Joel , WARD, Connie , HARRIS, Paul , JONES, Aubrey , YI, Jung
IPC分类号： C12N1/00
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467和1的一个或多个位置的取代，以及 486的SEQ ID NO：2的氨基酸1至513，并且任选地进一步包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

6. 发明公开

EP2987857A1 VARIANTS OF GLYCOSIDE HYDROLASES 审中-公开
标题翻译： VARIANTEN VON GLUCOSIDHYDROLASEN
公开(公告)号：EP2987857A1
公开(公告)日：2016-02-24
申请号：EP15180407.7
申请日：2004-08-25
申请人： Novozymes, Inc.
发明人： TETER, Sarah , CHERRY, Joel , WARD, Connie , HARRIS, Paul , JONES, Aubrey , YI, Jung
IPC分类号： C12N9/42 , C12N15/56 , C12P19/14
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467和1的一个或多个位置的取代，以及 486的SEQ ID NO：2的氨基酸1至513，并且任选地进一步包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

7. 发明公开

EP2069492A2 COMPOSITIONS FOR DEGRADING CELLULOSIC MATERIAL 审中-公开
标题翻译： ZUSAMMENSETZUNGEN ZUM ABBAU ZELLULOSEHALTIGER MATERIALIEN
公开(公告)号：EP2069492A2
公开(公告)日：2009-06-17
申请号：EP08769933.6
申请日：2008-05-30
申请人： Novozymes Inc.
发明人： MERINO, Sandra , MCFARLAND, Keith , CHERRY, Joel , TETER, Sarah
IPC分类号： C12N9/42 , C12N1/14 , C12P7/06
CPC分类号： C12N9/2437 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions. The compositions comprise (a) a polypeptide having cellulolytic enhancing activity (b) a beta glucosidase and (c) one or more cellulolytic enzymes selected from the group consisting of T. reesei cellohydrobiolase I T. reesei cellohydrobiolase II and T. reseei endoglucanase I and variants thereof.
摘要翻译：本发明涉及用于降解或转化含纤维素材料的纤维素分解组合物以及制备和使用该组合物的方法。

8. 发明授权

EP1766040B1 METHODS FOR TRANSFORMING AND EXPRESSION SCREENING OF FILAMENTOUS FUNGAL CELLS WITH A DNA LIBRARY 有权
标题翻译：用DNA库转化和表达丝状真菌细胞的表达筛选方法
公开(公告)号：EP1766040B1
公开(公告)日：2017-08-09
申请号：EP05786779.8
申请日：2005-05-26
申请人： Novozymes, Inc.
发明人： TETER, Sarah , LAMSA, Michael , CHEERY, Joel , WARD, Connie
IPC分类号： C12P21/06 , C12N1/16 , C12N15/74
CPC分类号： C12N15/1037 , C12N15/1086 , C12N15/80 , C40B40/02

9. 发明公开

EP2004817A2 POLYPEPTIDES HAVING ENDOGLUCANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
公开(公告)号：EP2004817A2
公开(公告)日：2008-12-24
申请号：EP07759868.8
申请日：2007-03-30
申请人： Novozymes, Inc. , Novozymes A/S
发明人： HARRIS, Paul , VLASENKO, Elena , KAUPPINNEN, Marcus Sakari , ZARETSKY, Elizabeth , TETER, Sarah , BROWN, Kimberly
IPC分类号： C12N9/42 , C12N15/82 , C12N15/56 , C07K14/37
CPC分类号： C12N9/244 , A61K38/00 , C07K14/5431 , C12N9/2437 , C12N15/1137 , C12P19/02 , C12P19/14 , C12P21/00 , C12Y302/01004 , Y02A50/473 , Y02E50/16
摘要： The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

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