专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20080241191A1 Process and materials for the rapid detection of streptococcus pneumoniae employing purified antigen-specific antibodies 审中-公开
标题翻译：使用纯化抗原特异性抗体快速检测肺炎链球菌的方法和材料
公开(公告)号：US20080241191A1
公开(公告)日：2008-10-02
申请号：US11982410
申请日：2007-10-31
申请人： Norman James Moore , Mary Kathleen Fent , Vladimir Andrei Koulchin , Elena Valentin Molokova
发明人： Norman James Moore , Mary Kathleen Fent , Vladimir Andrei Koulchin , Elena Valentin Molokova
IPC分类号： A61K39/09 , C07K16/12
CPC分类号： G01N33/56944 , C07K16/1275 , G01N21/78 , G01N2021/752 , G01N2021/757 , G01N2021/7759 , G01N2333/3156 , G01N2400/10 , G01N2469/10
摘要： Disclosed is a cell wall C-polysaccharide antigen of Streptococcus pneumoniae which contains not more than 10% by weight of protein, and preferably less which has been purified with 0.1N NaOH prior to deproteinizing. Also disclosed are polyvalent antibodies raised against Streptococcus pneumoniae which have been affinity purified by passing them over a chromatographic affinity matrix to which is coupled the purified and at least partially deproteinized antigen to render them antigen-specific.
摘要翻译：公开了肺炎链球菌的细胞壁C-多糖抗原，其含有不超过10重量％的蛋白质，并且优选较少，其在脱蛋白之前用0.1N NaOH纯化。还公开了针对肺炎链球菌产生的多价抗体，其通过将它们通过将纯化的和至少部分去蛋白质的抗原偶联以使其成为抗原特异性的色谱亲和基质将其亲和纯化。

2. 发明授权

US06824997B1 Process and materials for the rapid detection of streptococcus pneumoniae employing purified antigen-specific antibodies 有权
标题翻译：使用纯化抗原特异性抗体快速检测肺炎链球菌的方法和材料
公开(公告)号：US06824997B1
公开(公告)日：2004-11-30
申请号：US09397110
申请日：1999-09-16
申请人： Norman James Moore , Mary Kathleen Fent , Vladimir Andrei Koulchin , Elena Valentin Molokova
发明人： Norman James Moore , Mary Kathleen Fent , Vladimir Andrei Koulchin , Elena Valentin Molokova
IPC分类号： A61K39085
CPC分类号： G01N33/56944 , C07K16/1275 , G01N21/78 , G01N2021/752 , G01N2021/757 , G01N2021/7759 , G01N2333/3156 , G01N2400/10 , G01N2469/10
摘要： A process is disclosed for obtaining a C-polysaccharide cell wall antigen containing not more than about 10% protein from Streptococcus pneumoniae bacteria. The antigen thus obtained is conjugated to a spacer molecule, and the free end of the latter is then conjugated to a chromatographic affinity column. The column is then utilized to purify raw antibodies to S. pneumonia bacteria, thereby producing antigen-specific antibodies. A portion of such antibodies is conjugated to a labeling agent which displays a visible color change upon reaction of the antibodies with their antigenic binding partner and embedded in a first zone of an immunochromatographic assay device. Another portion of such antibodies is bound to the reaction zone of the device which has a view window. When a liquid sample, such as patient urine, cerebrospinal fluid or blood is applied to the first zone, the conjugate of antibodies and labeling agent and the sample move along a flow strip of bibulous material to the reaction zone wherein, if the sample contains S. pneumoniae or its cell wall antigen, a sandwich is formed among the labeled conjugate, the antigen and the bound antibodies and a color change is observed. The immunochromatographic assay thus performed is completed within about 15 minutes. This assay affords a basis for rapid and reliable diagnosis of various pathogenic states caused by S. pneumoniae including pneumonia, bronchitis, otitis media, sinusitis, meningitis, and secondary disease states that commonly occur when primary pneumonic infection caused by this bacterium persists undiminished over a time period of 3-5 days.
摘要翻译：公开了一种从肺炎链球菌细菌获得含有不超过约10％蛋白质的C-多糖细胞壁抗原的方法。由此获得的抗原与间隔分子缀合，然后将后者的自由末端与色谱亲和柱结合。然后将柱用于纯化对肺炎链球菌细菌的原始抗体，从而产生抗原特异性抗体。这种抗体的一部分与标记试剂缀合，所述标记试剂在抗体与其抗原结合配偶体反应并在免疫色谱测定装置的第一区域中显示可见的颜色变化。这种抗体的另一部分结合到具有视窗的装置的反应区。当将液体样品（例如患者尿液，脑脊液或血液）施加到第一区时，抗体和标记试剂的结合物和样品沿着吸水材料的流动条移动到反应区，其中如果样品含有S 肺炎支原体或其细胞壁抗原，在标记的缀合物，抗原和结合的抗体之间形成夹心，并观察到颜色变化。由此进行的免疫色谱测定在约15分钟内完成。该测定为快速可靠地诊断由肺炎链球菌引起的各种致病状态的基础，包括肺炎，支气管炎，中耳炎，鼻窦炎，脑膜炎，以及通常在由该细菌引起的原发性肺炎感染持续不减退时发生的继发性疾病状态时间为3-5天。

3. 发明申请

US20120135420A1 Method for detecting the presence of target bacteria or a target component carbohydrate antigen thereof 审中-公开
标题翻译：用于检测靶细菌或其靶组分碳水化合物抗原的存在的方法
公开(公告)号：US20120135420A1
公开(公告)日：2012-05-31
申请号：US11982400
申请日：2007-10-31
申请人： Vladimir Andrei Koulchin , Norman James Moore , Elena Valentin Molokova , Mary Kathleen Fent
发明人： Vladimir Andrei Koulchin , Norman James Moore , Elena Valentin Molokova , Mary Kathleen Fent
IPC分类号： G01N33/569 , C12P19/04 , G01N33/53 , C07K1/22 , C07K16/12
CPC分类号： C07K16/1242 , G01N33/558 , G01N33/56911 , G01N2333/28 , G01N2400/50
摘要： A process is disclosed for separating a carbohydrate antigen from a Gram-positive or Gram-negative bacteria in a purified form that contains no more than 10% protein. The separated antigen is coupled to an affinity column, over which polyclonal antibodies to the same bacteria are chromatographed and recovered in a purified form that exhibits high specificity and sensitivity in immunoassays for the raw carbohydrate antigen corresponding to the purified antigen on the column. A particularly preferred form of rapid immunochromatographic assay employing the purified antibodies, which assay is very useful as an aid to rapid diagnosis of diseases caused by bacteria, is disclosed.
摘要翻译：公开了以含有不超过10％蛋白质的纯化形式从革兰氏阳性或革兰氏阴性菌分离碳水化合物抗原的方法。将分离的抗原偶联到亲和柱上，通过该亲和柱将相同细菌的多克隆抗体以纯化形式进行色谱分离和回收，该纯化形式在对应于柱上纯化抗原的原始碳水化合物抗原的免疫测定中表现出高特异性和灵敏度。公开了一种特别优选的使用纯化抗体的快速免疫层析测定法，该测定法对于由细菌引起的疾病的快速诊断是非常有用的。

4. 发明授权

US09134303B1 ICT immunoassay for Legionella pneumophila serogroup 1 antigen employing affinity purified antibodies thereto 有权
标题翻译：使用亲和纯化抗体对嗜肺军团菌血清群1抗原进行ICT免疫测定
公开(公告)号：US09134303B1
公开(公告)日：2015-09-15
申请号：US09139720
申请日：1998-08-25
申请人： Vladimir Andrei Koulchin , Norman James Moore , Elena Valentin Molokova
发明人： Vladimir Andrei Koulchin , Norman James Moore , Elena Valentin Molokova
IPC分类号： C12Q1/68 , G01N33/00 , G01N33/569 , C12M3/00 , G01N33/53
CPC分类号： G01N33/56911 , C07K16/126 , G01N21/78 , G01N33/53 , G01N2021/752 , G01N2021/757 , G01N2021/7759 , G01N2400/10 , G01N2469/10
摘要： The present invention involves extracting from Legionella bacteria, particularly L. pneumophila bacteria, an essentially protein-free O-polysaccharide or carbohydrate antigen, coupling this antigen to an activated chromatographic column through a protein spacer molecule which is first conjugated to the antigen, utilizing the column thus prepared for the affinity purification of raw polyvalent antibodies to the same Legionella bacterium from which the O-polysaccharide or carbohydrate antigen was separated—thereby obtaining antigen-specific antibodies which are useful for the rapid detection of the corresponding Legionella bacterium or its antibody in human bodily fluids such as urine, sputum, blood and the like or in environmental samples suspected of harboring the Legionella bacterium.
摘要翻译：本发明涉及从军团菌细菌，特别是嗜肺链球菌细菌，一种基本上无蛋白的O-多糖或碳水化合物抗原提取，该抗原通过首先与抗原缀合的蛋白质间隔分子将该抗原与活化的色谱柱偶联，将这样制备的原料多价抗体亲和纯化到与其分离O-多糖或碳水化合物抗原的相同军团杆菌细菌中，从而获得可用于快速检测相应的军团菌或其抗体的抗原特异性抗体人体液体如尿液，痰液，血液等，或在怀疑携带军团杆菌细菌的环境样品中。

5. 发明授权

US06548309B1 Procedure for assay of liquids containing undissolved solids, semisolids or colloids 失效
标题翻译：含有不溶性固体，半固体或胶体的液体测定程序
公开(公告)号：US06548309B1
公开(公告)日：2003-04-15
申请号：US09044677
申请日：1998-03-19
申请人： Norman James Moore , Vincent Anthony Sy
发明人： Norman James Moore , Vincent Anthony Sy
IPC分类号： G01N33553
CPC分类号： G01N1/4077 , B01L3/5023 , B01L9/54 , B01L2300/0681 , B01L2300/0825 , G01N1/34 , Y10S436/808 , Y10S436/814 , Y10S436/818 , Y10S436/825
摘要： In qualitative or quantitative assays which employ a test device comprising a test strip pretreated for detection of a specified target analyte dissolved in a liquid medium, the presence of solid, semisolid and/or colloidal materials in the liquid medium may interfere with the assay results. The present invention involves collecting the sample from a flowing or quiescent pool of liquid also containing solid, semisolid or colloidal material with a swab comprised of a handle and a mass of fibrous material or foamed, open cell material which, when immersed in and thoroughly wetted by the liquid, entraps solid, semisolid and/or colloidal material and delivers only the liquid to the sample receiving zone of the test strip.
摘要翻译：在采用包含预处理以检测溶解在液体介质中的特定目标分析物的试纸的测试装置的定性或定量测定中，液体介质中固体，半固体和/或胶体材料的存在可能干扰测定结果。本发明涉及从具有固体，半固体或胶体材料的流动或静止液体的液体池中收集样品，其中包括手柄和一批纤维材料或泡沫的开孔细胞材料，当浸入并充分润湿通过液体，捕获固体，半固体和/或胶体材料，并仅将液体输送到测试条的样品接收区。

6. 发明授权

US09372191B2 Methods and devices for detecting methicillin resistant Staphylococcus aureus 有权
标题翻译：用于检测耐甲氧西林金黄色葡萄球菌的方法和装置
公开(公告)号：US09372191B2
公开(公告)日：2016-06-21
申请号：US12517771
申请日：2007-12-10
申请人： Dorothy Joanis , Myron Whipkey , Erin Hayes , Steven Muszynski , Alan H. Davis , Kristin Gordon , Roger N. Piasio , Norman James Moore
发明人： Dorothy Joanis , Myron Whipkey , Erin Hayes , Steven Muszynski , Alan H. Davis , Kristin Gordon , Roger N. Piasio , Norman James Moore
IPC分类号： G01N33/53 , C12Q1/18 , G01N33/569
CPC分类号： G01N33/56938 , G01N33/56944
摘要： Provided are methods and devices for detecting methicillin-resistant Staphylcoccus aureus.
摘要翻译：提供了用于检测耐甲氧西林金黄色葡萄球菌的方法和装置。

7. 发明申请

US20100304414A1 Methods and Devices for Detecting Methicillin Resistant Staphylococcus Aureus 有权
标题翻译：用于检测耐甲氧西林金黄色葡萄球菌的方法和装置
公开(公告)号：US20100304414A1
公开(公告)日：2010-12-02
申请号：US12517771
申请日：2007-12-10
申请人： Dorothy Joanis , Myron Whipkey , Erin Hayes , Steven Muszynski , Alan H. Davis , Kristin Gordon , Roger N. Piasio , Norman James Moore
发明人： Dorothy Joanis , Myron Whipkey , Erin Hayes , Steven Muszynski , Alan H. Davis , Kristin Gordon , Roger N. Piasio , Norman James Moore
IPC分类号： C12Q1/14 , C12Q1/04 , C12Q1/37
CPC分类号： G01N33/56938 , G01N33/56944
摘要： Provided are methods and devices for detecting methicillin-resistant Staphylcoccus aureus.
摘要翻译：提供了用于检测耐甲氧西林金黄色葡萄球菌的方法和装置。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式