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1. 发明申请

US20090137053A1 BEAD SET, PRODUCTION PROCESS OF THE BEAD SET, AND METHOD OF USING THE BEAD SET 审中-公开
标题翻译：珠组，珠组的生产过程以及使用珠组的方法
公开(公告)号：US20090137053A1
公开(公告)日：2009-05-28
申请号：US12108348
申请日：2008-04-23
申请人： Noriyuki Kishii , Mari Ichimura , Tomoteru Abe , Kazuhiro Nakagawa , Akio Yasuda , Mayumi Shiono , Michihiro Ohnishi , Takuro Yamamoto , Minoru Takeda
发明人： Noriyuki Kishii , Mari Ichimura , Tomoteru Abe , Kazuhiro Nakagawa , Akio Yasuda , Mayumi Shiono , Michihiro Ohnishi , Takuro Yamamoto , Minoru Takeda
IPC分类号： G01N33/50 , B05D3/00
CPC分类号： G01N21/645 , C12Q1/6869 , G01N33/54313 , Y10T436/143333 , C12Q2563/149
摘要： A bead set is composed of plural subsets of beads. The beads in each subset are each provided with a surface permitting immobilization thereon of a substance that takes part in a predetermined corresponding reaction or interaction. The individual beads in the beat set are provided with different physical elements, respectively, such that the individual beads in the bead can be distinguished into the plural subsets through an analysis of captured images of the individual beads in the bead set by a computer.
摘要翻译：珠组由多个珠子组成。每个子集中的珠粒各自具有允许在其上固定参与预定相应反应或相互作用的物质的表面。节拍组中的各个珠分别设置有不同的物理元件，使得可以通过分析由计算机设置的珠粒中的各个珠子的捕获图像来将珠粒中的各个珠子区分为多个子集。

2. 发明授权

US08691559B2 Micro channel, device for recovering nucleic acid and method for recovering nucleic acid 有权
标题翻译：微通道，用于回收核酸的装置和用于回收核酸的方法
公开(公告)号：US08691559B2
公开(公告)日：2014-04-08
申请号：US11858595
申请日：2007-09-20
申请人： Michihiro Ohnishi , Yuta Kan , Tomoteru Abe
发明人： Michihiro Ohnishi , Yuta Kan , Tomoteru Abe
IPC分类号： C12M1/34 , C12M3/00 , C12Q1/68 , B01D15/08 , C02F1/28 , C07H21/02 , C07H1/06
CPC分类号： C12N15/1006
摘要： A micro channel for recovering nucleic acid from a biological sample treated with a chaotropic ion, include an integrated portion composed of silica micro beads having a pore size of from 6 to 29 nm is formed in the channel.
摘要翻译：用离心力离子处理的生物样品回收核酸的微通道包括在通道中形成孔径为6〜29nm的二氧化硅微珠组成的整体部分。

3. 发明申请

US20070267336A1 Microflow path system 审中-公开
标题翻译：微流道系统
公开(公告)号：US20070267336A1
公开(公告)日：2007-11-22
申请号：US11798890
申请日：2007-05-17
申请人： Michihiro Ohnishi , Tomoteru Abe
发明人： Michihiro Ohnishi , Tomoteru Abe
IPC分类号： B01D15/08 , G01N30/02 , C12M1/34
CPC分类号： B01L3/502746 , B01L3/502753 , B01L3/561 , B01L2300/0681 , B01L2300/0838 , B01L2400/0487 , B01L2400/084 , G01N30/603
摘要： A microflow path system has a column layer of at least 0.5 mm in internal diameter. The column layer includes a hybrid-forming section and a fluid-supply promoting section arranged in a region downstream of the hybrid-forming section. The hybrid-forming section is packed with beads to provide a bead bed length of not longer than 4 mm. The fluid-supply promoting section serves to contribute to an increase in a flow rate of a fluid supply.
摘要翻译：微流路系统具有内径至少为0.5mm的柱层。柱层包括混合形成部分和设置在混合成形部分下游的区域中的流体供应促进部分。混合成形部分装有珠，以提供不超过4mm的珠床长度。流体供应促进部分有助于增加流体供应的流量。

4. 发明授权

US09523091B2 Nucleic-acid extraction method and nucleic-acid extraction cartridge 有权
标题翻译：核酸提取方法和核酸提取盒
公开(公告)号：US09523091B2
公开(公告)日：2016-12-20
申请号：US13533348
申请日：2012-06-26
申请人： Tasuku Yotoriyama , Tomohiko Nakamura , Naohisa Sakamoto , Kazumine Ito , Tomoteru Abe , Michihiro Ohnishi
发明人： Tasuku Yotoriyama , Tomohiko Nakamura , Naohisa Sakamoto , Kazumine Ito , Tomoteru Abe , Michihiro Ohnishi
IPC分类号： C07H21/00 , B01D59/42 , C12N15/10
CPC分类号： C12N15/1006 , C12N15/101
摘要： The present disclosure provides a nucleic-acid extraction method for carrying out the following procedures in the same cell, the procedures including: performing an ultrasonic process on a sample containing a nucleic acid; adsorbing substances contained in the sample by making use of an adsorption carrier; and condensing the nucleic acid by damming the nucleic acid moving in an electrophoresis phenomenon. In accordance with the nucleic-acid extraction method, it is possible to carry out an ultrasonic process, an adsorption process and an electrophoresis process in the same cell. Thus, it is possible to eliminate an operation to transfer the sample from one cell to another one for each of the processes.
摘要翻译：本公开内容提供了用于在相同细胞中进行以下程序的核酸提取方法，其方法包括：对含有核酸的样品进行超声波处理; 通过使用吸附载体吸附样品中所含的物质; 并通过阻止以电泳现象移动的核酸来冷凝核酸。根据核酸提取方法，可以在同一电池中进行超声波处理，吸附处理和电泳处理。因此，可以消除用于每个处理将样品从一个单元传送到另一个单元的操作。

5. 发明申请

US20080161553A1 MICRO CHANNEL, DEVICE FOR RECOVERING NUCLEIC ACID AND METHOD FOR RECOVERING NUCLEIC ACID 有权
标题翻译：微通道，用于回收核酸的装置和用于回收核酸的方法
公开(公告)号：US20080161553A1
公开(公告)日：2008-07-03
申请号：US11858595
申请日：2007-09-20
申请人： Michihiro Ohnishi , Yuta Kan , Tomoteru Abe
发明人： Michihiro Ohnishi , Yuta Kan , Tomoteru Abe
IPC分类号： C07H1/06 , C12M1/00 , C40B60/14
CPC分类号： C12N15/1006
摘要： A micro channel for recovering nucleic acid from a biological sample treated with a chaptropic ion, include an integrated portion composed of silica micro beads having a pore size of from 6 to 29 nm is formed in the channel.
摘要翻译：用于从由chaptropic离子处理的生物样品中回收核酸的微通道包括在通道中形成具有6至29nm孔径的二氧化硅微珠组成的整体部分。

6. 发明申请

US20130020201A1 NUCLEIC-ACID EXTRACTION METHOD AND NUCLEIC-ACID EXTRACTION CARTRIDGE 有权
标题翻译：核酸提取方法和核酸提取盒
公开(公告)号：US20130020201A1
公开(公告)日：2013-01-24
申请号：US13533348
申请日：2012-06-26
申请人： Tasuku Yotoriyama , Tomohiko Nakamura , Naohisa Sakamoto , Kazumine Ito , Tomoteru Abe , Michihiro Ohnishi
发明人： Tasuku Yotoriyama , Tomohiko Nakamura , Naohisa Sakamoto , Kazumine Ito , Tomoteru Abe , Michihiro Ohnishi
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： C12N15/1006 , C12N15/101
摘要： The present disclosure provides a nucleic-acid extraction method for carrying out the following procedures in the same cell, the procedures including: performing an ultrasonic process on a sample containing a nucleic acid; adsorbing substances contained in the sample by making use of an adsorption carrier; and condensing the nucleic acid by damming the nucleic acid moving in an electrophoresis phenomenon. In accordance with the nucleic-acid extraction method, it is possible to carry out an ultrasonic process, an adsorption process and an electrophoresis process in the same cell. Thus, it is possible to eliminate an operation to transfer the sample from one cell to another one for each of the processes.
摘要翻译：本公开内容提供了用于在相同细胞中进行以下程序的核酸提取方法，其方法包括：对含有核酸的样品进行超声波处理; 通过使用吸附载体吸附样品中所含的物质; 并通过阻止以电泳现象移动的核酸来冷凝核酸。根据核酸提取方法，可以在同一电池中进行超声波处理，吸附处理和电泳处理。因此，可以消除用于每个处理将样品从一个单元传送到另一个单元的操作。

7. 发明申请

US20090275743A1 METHOD OF PREPARING CARRIER TO SEPARATE NUCLEIC ACIDS, CARRIER AND MICRO CHANNEL TO SEPARATE NUCLEIC ACIDS, AND METHOD AND APPARATUS FOR SEPARATING NUCLEIC ACIDS 有权
标题翻译：制备分离核酸的载体的方法，分离核酸的载体和微通道的方法以及分离核酸的方法和装置
公开(公告)号：US20090275743A1
公开(公告)日：2009-11-05
申请号：US12432078
申请日：2009-04-29
申请人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
发明人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
IPC分类号： C07H1/00 , B01J19/00 , B05D3/00
CPC分类号： C12N15/1006
摘要： The present invention provides a method for producing a carrier for nucleic acid separation, the method including a step of: immobilizing capturing chains onto a porous carrier having cation-exchangeable groups thereon by ion exchange bonding, the capturing chains having a base sequence complementary to target nucleic acid chains and being modified with functional groups having positive charges.
摘要翻译：本发明提供了一种核酸分离用载体的制造方法，该方法包括以下步骤：通过离子交换键将捕获链固定在其上具有阳离子交换基团的多孔载体上，捕获链具有与靶相互补充的碱基序列核酸链，并用具有正电荷的官能团修饰。

8. 发明授权

US08785348B2 Method of preparing carrier to separate nucleic acids, carrier and micro channel to separate nucleic acids, and method and apparatus for separating nucleic acids 有权
标题翻译：制备分离核酸，载体和微通道以分离核酸的载体的方法，以及用于分离核酸的方法和装置
公开(公告)号：US08785348B2
公开(公告)日：2014-07-22
申请号：US12432078
申请日：2009-04-29
申请人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
发明人： Michihiro Ohnishi , Noriyuki Kishii , Takuya Kishimoto , Naoyuki Sasaki , Hidetoshi Watanabe
IPC分类号： B01J20/00 , B01J23/02 , C07H21/00
CPC分类号： C12N15/1006
摘要： The present disclosure relates to a method and apparatus for separating nucleic acids. A carrier may include a porous microbead having cation-exchangeable groups attached to the surface of the porous microbead. Capturing chains modified with positively charged functional groups and having a base sequence complementary to a target nucleic acid chain sequence are immobilized on to the surface of the porous microbead. In various embodiments, capturing chains are immobilized on to the surface of the porous microbead through an ion exchange bond or a covalent bond with the cation-exchangeable groups of the porous microbead. In some cases, the porous microbead has a number of through pores adapted to permit a solution to pass rapidly through the through pores and a number of diffusive pores adapted to permit a solute of the solution to diffuse into the diffusive pores. A solution having a target nucleic acid chain corresponding to the target nucleic acid chain sequence of the capturing chains may be passed through a channel that is packed with porous microbeads so as to result in hybridization of the capturing chain and the target nucleic acid chain. The porous microbeads may be structured such that the pressure in the channel upon passing of the solution through the channel does not build significantly.
摘要翻译：本公开涉及用于分离核酸的方法和装置。载体可以包括具有连接到多孔微珠表面的可阳离子交换基团的多孔微珠。用正电荷的官能团修饰并具有与靶核酸链序列互补的碱基序列的捕获链被固定在多孔微珠的表面上。在各种实施方案中，捕获链通过与多孔微珠的可阳离子交换基团的离子交换键或共价键固定在多孔微珠的表面上。在一些情况下，多孔微珠具有适于使溶液快速通过通孔的多个通孔，并且适于允许溶液的溶质扩散到扩散孔中的许多扩散孔。具有对应于捕获链的靶核酸链序列的靶核酸链的溶液可以通过填充有多孔微珠的通道，从而导致捕获链和靶核酸链的杂交。多孔微珠可以被构造成使得溶液通过通道时通道中的压力不会显着增加。

9. 发明申请

US20080299541A1 METHOD AND APPARATUS FOR MEASURING ENZYMATIC ACTIVITY BY USE OF LASER 失效
标题翻译：通过使用激光测量酶活性的方法和装置
公开(公告)号：US20080299541A1
公开(公告)日：2008-12-04
申请号：US12110969
申请日：2008-04-28
申请人： Takuya Kishimoto , Hidetoshi Watanabe , Michihiro Ohnishi
发明人： Takuya Kishimoto , Hidetoshi Watanabe , Michihiro Ohnishi
IPC分类号： C12Q1/00 , C12M1/00
CPC分类号： A61B5/0059 , A61B5/0071 , A61B5/441
摘要： A method of measuring an enzymatic activity, includes measuring the quantity of a substrate metabolite produced upon metabolism of a substrate by an enzyme, through detecting a radiant wave generated from a multiple photon excitation process of the substrate or the substrate metabolite.
摘要翻译：测量酶活性的方法包括通过检测由底物或底物代谢物的多光子激发过程产生的辐射波来测量由酶代谢基质时产生的底物代谢物的量。

10. 发明申请

US20060216816A1 Flow path system provided with reaction section suited for the detection of hybridization, and hybridization detecting device making use of the flow path system 审中-公开
标题翻译：提供了适用于检测杂交的反应段的流路系统，以及利用流路系统的杂交检测装置
公开(公告)号：US20060216816A1
公开(公告)日：2006-09-28
申请号：US11378361
申请日：2006-03-20
申请人： Michihiro Ohnishi
发明人： Michihiro Ohnishi
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01L3/502746 , B01L3/561 , B01L2300/0681 , B01L2300/0838 , B01L2400/0487 , B01L2400/084 , C12Q1/6837 , G01N30/02 , B01D15/345
摘要： A flow path system suitable for use in the detection of hybridization includes a capillary provided at a predetermined location thereof with a reaction section packed with beads. On the beads, a linker having a sequence of bases of the same type is immobilized, and a target nucleic acid complementarily bound to the sequence of the bases of the same type is held.
摘要翻译：适用于检测杂交的流路系统包括在其预定位置处提供的填充有珠粒的反应段的毛细管。在珠粒上，固定具有相同碱基序列的接头，并保持与相同碱基序列互补结合的靶核酸。

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