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1. 发明授权

US06307029B1 Dye-labeled protein conjugate and method for preparing the same 失效
标题翻译：染料标记的蛋白质缀合物及其制备方法
公开(公告)号：US06307029B1
公开(公告)日：2001-10-23
申请号：US09311147
申请日：1999-05-13
申请人： Nobuyuki Shigetou , Jinsei Miyazaki , Hiroshi Nakayama
发明人： Nobuyuki Shigetou , Jinsei Miyazaki , Hiroshi Nakayama
IPC分类号： C07K1702
CPC分类号： G01N33/532 , G01N33/531 , Y10S436/80
摘要： The present invention provides a dye-labeled protein conjugate in which a protein conjugate is labeled with a large number of dye molecules. In the dye-labeled protein conjugate, a protein conjugate that includes a protein and an antibody bound thereto via a disulfide bond is labeled with a cyanine dye represented by the formula (1) or the formula (2) given below: where R1 and R2 denote hydrogen or an alkyl group, X denotes a halogen, M denotes hydrogen or an alkali metal, and n represents an integer in a range of 1 to 4.
摘要翻译：本发明提供染料标记的蛋白质缀合物，其中蛋白质缀合物用大量染料分子标记。在染料标记的蛋白质缀合物中，包含蛋白质和通过二硫键结合的抗体的蛋白质缀合物用下面给出的式（1）或式（2）表示的花青染料标记：其中R1和R2 表示氢或烷基，X表示卤素，M表示氢或碱金属，n表示1〜4的整数。

2. 发明授权

US06300480B1 Dye-labeled protein conjugate and method for preparing the same 失效
标题翻译：染料标记的蛋白质缀合物及其制备方法
公开(公告)号：US06300480B1
公开(公告)日：2001-10-09
申请号：US09310851
申请日：1999-05-13
申请人： Nobuyuki Shigetou , Jinsei Miyazaki , Hiroshi Nakayama
发明人： Nobuyuki Shigetou , Jinsei Miyazaki , Hiroshi Nakayama
IPC分类号： C07K1702
CPC分类号： G01N33/532 , G01N33/531 , Y10S436/80
摘要： The present invention provides a dye-labeled protein conjugate in which a protein conjugate is labeled with a large number of dye molecules. In the dye-labeled protein conjugate, a protein conjugate that includes a protein and an antibody bound thereto via a disulfide bond is labeled with a cyanine dye represented by the formula (1) given below. where R1 and R2 denote hydrogen or an alkyl group, X denotes a halogen, M denotes hydrogen or an alkali metal, and n represents an integer in a rage of 1 to 4.
摘要翻译：本发明提供染料标记的蛋白质缀合物，其中蛋白质缀合物用大量染料分子标记。在染料标记的蛋白质缀合物中，包含蛋白质和通过二硫键结合的抗体的蛋白质缀合物用下述式（1）表示的花青染料标记。其中R1和R2表示氢或烷基 X表示卤素，M表示氢或碱金属，n表示1〜4的整数。

3. 发明授权

US07790470B2 Stirring method, cell, and measuring apparatus using the same 有权
标题翻译：搅拌方法，电池和使用其的测量装置
公开(公告)号：US07790470B2
公开(公告)日：2010-09-07
申请号：US10588546
申请日：2005-03-23
申请人： Akihito Kamei , Fumihisa Kitawaki , Tatsurou Kawamura , Hiroshi Nakayama , Nobuyuki Shigetou
发明人： Akihito Kamei , Fumihisa Kitawaki , Tatsurou Kawamura , Hiroshi Nakayama , Nobuyuki Shigetou
IPC分类号： G01N1/00 , G01N33/00 , G01N21/00 , G01N15/06 , G01N33/53
CPC分类号： B01F5/0057 , B01F1/0022 , B01F5/0206 , B01F2215/0037 , G01N21/03 , G01N21/76 , G01N2035/00544 , Y10T436/25
摘要： A method capable of stirring a liquid sample and a reagent promptly and easily includes the following steps (A) and (B). In step (A), using a cell including: a liquid sample retaining section having a plurality of particles; and a liquid sample supply inlet, a liquid sample containing an analyte is supplied from the liquid sample supply inlet to the liquid sample retaining section. In step (B), the liquid sample and a specific binding substance capable of specifically binding to the analyte are stirred by the movement of the particles caused by the flow of the liquid sample in the cell resulting from the supply of the liquid sample, to obtain a liquid mixture. The electric charge of at least the surface of the particles and the electric charge of the specific binding substance have the same polarity in the liquid mixture.
摘要翻译：能够迅速且容易地搅拌液体样品和试剂的方法包括以下步骤（A）和（B）。在步骤（A）中，使用包括：具有多个颗粒的液体样品保留部分的细胞; 将液体试样供给口从液体试样供给口供给到液体试样保持部。在步骤（B）中，液体样品和能够特异性结合分析物的特异性结合物质由于由液体样品供应而导致的液体样品流动引起的颗粒运动而被搅拌至获得液体混合物。至少颗粒表面的电荷和特定结合物质的电荷在液体混合物中具有相同的极性。

4. 发明申请

US20070172961A1 Stirring method, cell, and measuring apparatus using the same 有权
标题翻译：搅拌方法，电池和使用其的测量装置
公开(公告)号：US20070172961A1
公开(公告)日：2007-07-26
申请号：US10588546
申请日：2005-03-23
申请人： Akihito Kamei , Fumihisa Kitawaki , Tatsurou Kawamura , Hiroshi Nakayama , Nobuyuki Shigetou
发明人： Akihito Kamei , Fumihisa Kitawaki , Tatsurou Kawamura , Hiroshi Nakayama , Nobuyuki Shigetou
IPC分类号： G01N1/00
CPC分类号： B01F5/0057 , B01F1/0022 , B01F5/0206 , B01F2215/0037 , G01N21/03 , G01N21/76 , G01N2035/00544 , Y10T436/25
摘要： A method capable of stirring a liquid sample and a reagent promptly and easily includes the following steps (A) and (B). In step (A), using a cell including: a liquid sample retaining section having a plurality of particles; and a liquid sample supply inlet, a liquid sample containing an analyte is supplied from the liquid sample supply inlet to the liquid sample retaining section. In step (B), the liquid sample and a specific binding substance capable of specifically binding to the analyte are stirred by the movement of the particles caused by the flow of the liquid sample in the cell resulting from the supply of the liquid sample, to obtain a liquid mixture. The electric charge of at least the surface of the particles and the electric charge of the specific binding substance have the same polarity in the liquid mixture.
摘要翻译：能够迅速且容易地搅拌液体样品和试剂的方法包括以下步骤（A）和（B）。在步骤（A）中，使用包括：具有多个颗粒的液体样品保留部分的细胞; 将液体试样供给口从液体试样供给口供给到液体试样保持部。在步骤（B）中，液体样品和能够特异性结合分析物的特异性结合物质由于由液体样品供应而导致的液体样品流动引起的颗粒运动而被搅拌至获得液体混合物。至少颗粒表面的电荷和特定结合物质的电荷在液体混合物中具有相同的极性。

5. 发明授权

US5378634A Labelling color for detecting methamphetamine 失效
标题翻译：用于检测甲基苯丙胺的标签颜色
公开(公告)号：US5378634A
公开(公告)日：1995-01-03
申请号：US102426
申请日：1993-08-05
申请人： Nobuyuki Sigetoh , Jinsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
发明人： Nobuyuki Sigetoh , Jinsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
IPC分类号： C09B23/08 , G01N33/58 , G01N33/00
CPC分类号： C09B23/083 , G01N33/582 , Y10S435/968 , Y10S436/816 , Y10S436/901 , Y10T436/14
摘要： A labelling color which includes a pentamethine of the following formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 each are a C.sub.1-6 alkyl group, and X is an anion.
摘要翻译：包括下式（1）的五甲氧基的标记颜色：其中R 1，R 2，R 3和R 4各自为C 1-6烷基，X为阴离子。

6. 发明授权

US06448018B1 Fluorescence polarization method at multiple wavelengths 有权
标题翻译：多波长荧光偏振法
公开(公告)号：US06448018B1
公开(公告)日：2002-09-10
申请号：US09346817
申请日：1999-07-02
申请人： Hiroshi Nakayama , Fumihisa Kitawaki , Jinsei Miyazaki
发明人： Hiroshi Nakayama , Fumihisa Kitawaki , Jinsei Miyazaki
IPC分类号： G01N3353
CPC分类号： G01N21/6445
摘要： A method of the present invention is a fluorescence polarization method at multiple wavelengths for analyzing two or more different assay-objects in a sample. The method includes the steps of: (a) providing two or more different fluorescent-labeled substances, each being a substance which is capable of specifically binding to respective one of the assay-objects and is covalently bound to a fluorochrome, wherein the fluorochromes of the fluorescent-labeled substances are different from one another; (b) allowing the fluorescent-labeled substances to bind to the two or more different assay-objects, respectively; and (c) measuring a change in the degree of fluorescence polarization which has taken place in each of the fluorescent-labeled substances by its binding to one of the assay-objects.
摘要翻译：本发明的方法是用于分析样品中两种或更多种不同测定对象的多个波长的荧光偏振方法。该方法包括以下步骤：（a）提供两种或更多种不同的荧光标记的物质，每种物质是能够特异性结合相应的一种测定对象并共价结合荧光染料的物质，其中荧光染料荧光标记物质彼此不同; （b）使荧光标记的物质分别与两种或多种不同的测定对象结合; 和（c）通过与其中一个测定对象结合来测量在每个荧光标记物质中发生的荧光偏振度的变化。

7. 发明授权

US5965713A Dye labeled protein conjugate its preparing method and sensor using the same 失效
标题翻译：染料标记的蛋白质缀合物其制备方法和使用其的传感器
公开(公告)号：US5965713A
公开(公告)日：1999-10-12
申请号：US745337
申请日：1996-11-08
申请人： Nobuyuki Shigeto , Jinsei Miyazaki , Hiroshi Nakayama
发明人： Nobuyuki Shigeto , Jinsei Miyazaki , Hiroshi Nakayama
IPC分类号： G01N33/532 , C07K16/00 , C09B23/00 , G01N33/533 , A61K39/385
CPC分类号： G01N33/533 , C07K16/00 , Y10S530/802 , Y10S530/861 , Y10S530/866 , Y10T436/14
摘要： It is intended to present a sensor capable of labeling with more dyes, applying the labeled protein in immunochromatography making use of antigen-antibody reaction, and having an excellent sensitivity. In a buffer, a protein and a first covalent bonding compound that can react with this protein are reacted to prepare a protein conjugate, then a cyanine labeling dye is added in the buffer containing the protein conjugate, and the protein conjugate and cyanine labeling dye are reacted to prepare a dye labeled protein conjugate. Alternatively, in a buffer solution, a protein and a cyanine labeling dye are reacted to prepare a dye labeled protein, then a first covalent bonding compound that can react with the protein is added in the buffer containing the dye labeled protein, and the dye labeled protein and first covalent bonding protein are reacted to prepare a dye labeled protein conjugate.
摘要翻译：旨在提供能够用更多染料标记的传感器，在使用抗原 - 抗体反应的免疫色谱法中应用标记的蛋白质并具有优异的灵敏度。在缓冲液中，使蛋白质和可与该蛋白质反应的第一共价键合化合物进行反应以制备蛋白质缀合物，然后在含有蛋白质缀合物的缓冲液中加入花青标记染料，蛋白质缀合物和花青标记染料为反应以制备染料标记的蛋白质缀合物。或者，在缓冲溶液中，使蛋白质和花青标记染料反应以制备染料标记的蛋白质，然后将可与蛋白质反应的第一共价键合化合物加入到含有染料标记蛋白质的缓冲液中，染料标记蛋白质和第一共价键合蛋白反应以制备染料标记的蛋白质缀合物。

8. 发明授权

US06432632B1 Fluorescence polarization method 有权
公开(公告)号：US06432632B1
公开(公告)日：2002-08-13
申请号：US09297603
申请日：1999-07-23
申请人： Hiroshi Nakayama , Jinsei Miyazaki
发明人： Hiroshi Nakayama , Jinsei Miyazaki
IPC分类号： C12Q170
摘要： A fluorescence polarization method for analyzing an assay-object in a sample is provided. The fluorescence polarization method includes the steps of: (a) providing a fluorescent-labeled protein in which a protein is covalently bound to a fluorochrome(s), wherein the protein is capable of specifically binding to the assay-object; (b) allowing the fluorescent-labeled protein to bind to the assay-object; and (c) measuring a change in the degree of fluorescence polarization which has taken place in the fluorescent-labeled protein by its binding to the assay-object.

9. 发明授权

US5571727A Labelling colors for detecting cocaine or methamphetamine, method of preparing the same and detector for cocaine or methamphetamine 失效
标题翻译：用于检测可卡因或甲基苯丙胺的标签颜色，其制备方法和可卡因或甲基苯丙胺检测器
公开(公告)号：US5571727A
公开(公告)日：1996-11-05
申请号：US319976
申请日：1994-10-07
申请人： Nobuyuki Shigetoh , Hiroshi Nakayama , Jinsei Miyazaki , Tadayasu Mitsumata
发明人： Nobuyuki Shigetoh , Hiroshi Nakayama , Jinsei Miyazaki , Tadayasu Mitsumata
IPC分类号： C07D209/14 , C07D471/08 , G01N33/532 , G01N33/542 , G01N33/94 , C07D401/06 , G01N33/533
CPC分类号： C07D471/08 , C07D209/14 , G01N33/532 , G01N33/542 , G01N33/946 , Y10S436/816
摘要： A labelling color for detecting cocaine of Formula (I) ##STR1## wherein X represents a halogen, and a method of detecting methamphetamine, including the steps of adding a labelling color of Formula (II) ##STR2## wherein X represents an anion to a first solution of methamphetamine antibody to form a labelling color-methamphetamine antibody complex, measuring the fluorescence of the first solution, adding a sample containing an unknown amount of methamphetamine to the first solution to form a second solution, measuring the fluorescence of the second solution, and correlating any change in the fluorescence between the first and second solutions to the concentration of methamphetamine in the sample.
摘要翻译：用于检测式（I）的可卡因的标记颜色其中X表示卤素的式（I）和检测甲基苯丙胺的方法，包括以下步骤：添加式（II）的标记颜色），其中X表示甲基苯丙胺抗体的第一溶液的阴离子，以形成标记颜色 - 甲基苯丙胺抗体复合物，测量第一溶液的荧光，向第一溶液中加入含有未知量的甲基苯丙胺的样品以形成第二溶液，测量第二溶液的荧光，并将第一和第二溶液之间的荧光的任何变化与样品中甲基苯丙胺的浓度相关联。

10. 发明授权

US08631152B2 System and method for data packet transmission and reception 失效
标题翻译：数据包发送和接收的系统和方法
公开(公告)号：US08631152B2
公开(公告)日：2014-01-14
申请号：US12385885
申请日：2009-04-22
申请人： Hidekazu Osano , Hiroshi Nakayama
发明人： Hidekazu Osano , Hiroshi Nakayama
IPC分类号： G06F15/16
CPC分类号： G06F13/387
摘要： A system transmits a data packet from a transmitting apparatus to a receiving apparatus. The receiving apparatus includes a receive buffer, and a size specifying information transmitting unit that transmits size specifying information to the transmitting apparatus. The transmitting apparatus includes a transmit buffer, a credit storage unit that stores, as a credit, a value corresponding to a total size of all data packets stored in the receive buffer, a credit adding unit that adds a credit to the stored credit on transmitting a data packet, a credit subtracting unit that specifies a size of a read-out data packet on receiving the size specifying information, subtracts a credit corresponding to the specified size from a stored credit, and a transmission controlling unit that controls data packet transmission based on a credit stored in the credit storage unit.
摘要翻译：系统将数据分组从发送装置发送到接收装置。接收装置包括接收缓冲器和向发送装置发送大小指定信息的大小指定信息发送单元。发送装置包括发送缓冲器，信用存储单元，作为信用存储对应于存储在接收缓冲器中的所有数据分组的总大小的值;信用增加单元，用于向所发送的存储信用增加信用数据分组，信用减除单元，其在接收到大小指定信息时指定读出数据分组的大小，从存储的积分中减去与规定大小相对应的信用，以及控制数据分组发送的发送控制部，存储在信用存储单元中的信用。

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