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    • 3. 发明授权
    • Method of modifying target region in host DNA and selectable marker cassette
    • 修改宿主DNA和选择性标记盒中靶区的方法
    • US08852943B2
    • 2014-10-07
    • US12933766
    • 2009-03-23
    • Katsutoshi AraTakuya MorimotoNaotake Ogasawara
    • Katsutoshi AraTakuya MorimotoNaotake Ogasawara
    • C12N15/70C12N15/74C12N15/10C12N1/21C07H21/04
    • C12N15/1082C12N15/102
    • A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5′-side region outside of the target region in the host DNA, a 3′-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3′-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5′-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3′-side region derived from the host DNA and the 3′-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter; and a selectable marker cassette for use in the method.
    • 使用供体DNA修饰宿主DNA中的靶区域的方法:其中具有与宿主DNA中的靶区域外侧的5'-侧区域同源的区域的供体DNA,靶标外的3'侧区域 区域,宿主DNA中的靶区域内的第一同源重组区域,并且还具有第一选择标记基因,表达诱导启动子和第二选择标记基因 控制与3'侧区域同源的区域与与第一同源重组区域同源的区域之间的表达诱导启动子; 该方法具有以下步骤:在5'-侧区域和第一同源重组区域的区域上进行供体DNA和宿主DNA之间的同源重组的第一步骤,以进行与供体DNA整合的宿主的选择 基于第一选择标记基因的表达; 以及在源自宿主DNA的3'-侧区域的两个区域和源自供体DNA的3'侧区域之间,通过第1步在与供体DNA整合的宿主DNA内进行同源重组的第2步骤 基于表达诱导启动子的表达诱导条件进行基于第二选择标记基因的表达修饰其靶区的宿主的选择; 以及用于该方法的可选标记盒。
    • 5. 发明申请
    • CIS-BASED THIN FILM SOLAR CELL
    • 基于CIS的薄膜太阳能电池
    • US20130146137A1
    • 2013-06-13
    • US13702458
    • 2011-06-16
    • Takuya MorimotoHiroki SugimotoHideki Hakuma
    • Takuya MorimotoHiroki SugimotoHideki Hakuma
    • H01L31/0224
    • H01L31/022441H01L31/022425H01L31/0322H01L31/046H01L31/0463H01L31/0749Y02E10/541
    • A CIS-based thin film solar cell has a backside electrode layer that is divided by a pattern (P1), and a CIS-based light absorption layer, and a transparent conductive film are sequentially formed on a substrate. The backside electrode layer comprises an intermediate layer on the surface that is in contact with the CIS-based light absorption layer, the intermediate layer being composed of a compound of a metal that constitutes the backside electrode layer and a group VI element that constitutes the CIS-based light absorption layer; the intermediate layer comprises a first intermediate layer portion which is formed on the upper surface and a second intermediate layer portion which is formed on the lateral surface that and faces the pattern (P1); and the film thickness of the second intermediate layer portion is larger than the film thickness of the first intermediate layer portion.
    • 基于CIS的薄膜太阳能电池具有由图案(P1)分割的背面电极层,并且在基板上依次形成CIS系的光吸收层和透明导电膜。 背面电极层包括与基于CIS的光吸收层接触的表面上的中间层,中间层由构成背面电极层的金属的化合物和构成CIS的第VI族元素组成 基光吸收层; 中间层包括形成在上表面上的第一中间层部分和形成在面向图案(P1)的侧表面上的第二中间层部分; 并且第二中间层部分的膜厚度大于第一中间层部分的膜厚度。
    • 7. 发明申请
    • NONAQUEOUS ELECTROLYTE SECONDARY BATTERY
    • 非电解电解质二次电池
    • US20100190064A1
    • 2010-07-29
    • US12690981
    • 2010-01-21
    • Yoshihiko IkedaTakuya MorimotoHideyuki Inomata
    • Yoshihiko IkedaTakuya MorimotoHideyuki Inomata
    • H01M6/16
    • H01M10/0525H01M10/0567H01M2004/021H01M2004/028Y02T10/7011
    • The nonaqueous electrolyte secondary battery uses a positive electrode active material which is a mixture of large particle diameter-positive electrode active material particles having a central particle diameter D50 of 15 to 30 μm and small particle diameter-positive electrode active material particles having a central particle diameter D50 of 1 to 8 μm, in which the particle size distribution has a peak having a relative particle amount of 5% or more in each of a particle diameter range of 15 to 30 μm and a particle diameter range of 1 to 8 μm, and the nonaqueous electrolyte contains 1,3-dioxane, a vinylene carbonate compound, and at least one type of aromatic compound selected from a cycloalkylbenzene compound and a compound having a quaternary carbon adjacent to a benzene ring, thus having high safety when overcharged, a large initial capacity and excellent charging-discharging cycle characteristics and generating less gas.
    • 非水电解质二次电池使用正极活性物质,其是具有中心粒径D50为15〜30μm的大粒径 - 正极活性物质粒子和具有中心粒子的小粒径 - 正极活性物质粒子的混合物 直径D50为1〜8μm,粒径分布在粒径为15〜30μm,粒径为1〜8μm的范围内具有相对粒径为5%以上的峰, 非水电解质含有1,3-二恶烷,碳酸亚乙烯酯化合物和选自环烷基苯化合物和具有与苯环相邻的季碳的化合物中的至少一种芳族化合物,因此当过充电时具有高安全性, 初始容量大,充放电循环特性优异,产气少。
    • 8. 发明申请
    • Method of Modifying Target Region in Host DNA and Selectable Marker Cassette
    • 修改宿主DNA和可选择标记盒中目标区域的方法
    • US20110014709A1
    • 2011-01-20
    • US12933766
    • 2009-03-23
    • Katsutoshi AraTakuya MorimotoNaotake Ogasawara
    • Katsutoshi AraTakuya MorimotoNaotake Ogasawara
    • C12N15/87C12N15/63
    • C12N15/1082C12N15/102
    • A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5′-side region outside of the target region in the host DNA, a 3′-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3′-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5′-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3′-side region derived from the host DNA and the 3′-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter: and a selectable marker cassette for use in the method.
    • 使用供体DNA修饰宿主DNA中的靶区域的方法:其中具有与宿主DNA中的靶区域外侧的5'-侧区域同源的区域的供体DNA,靶标外的3'侧区域 区域,宿主DNA中的靶区域内的第一同源重组区域,并且还具有第一选择标记基因,表达诱导启动子和第二选择标记基因 控制与3'侧区域同源的区域与与第一同源重组区域同源的区域之间的表达诱导启动子; 该方法具有以下步骤:在5'-侧区域和第一同源重组区域的区域上进行供体DNA和宿主DNA之间的同源重组的第一步骤,以进行与供体DNA整合的宿主的选择 基于第一选择标记基因的表达; 以及在源自宿主DNA的3'-侧区域的两个区域和源自供体DNA的3'侧区域之间,通过第1步在与供体DNA整合的宿主DNA内进行同源重组的第2步骤 ,用于在表达诱导启动子的表达诱导条件下,基于第二选择标记基因的表达来选择其目标区域被修饰的宿主;以及用于该方法的可选择标记盒。
    • 9. 发明授权
    • Method for producing a secondary cell having flat wound electrode body
    • 具有扁平卷绕电极体的二次电池的制造方法
    • US07695530B2
    • 2010-04-13
    • US11269880
    • 2005-11-09
    • Yasuhiro YamauchiTomokazu YamanakaTakuya Morimoto
    • Yasuhiro YamauchiTomokazu YamanakaTakuya Morimoto
    • H01M4/82H01M6/12
    • H01M10/0431H01M4/133H01M10/0525H01M10/0587H01M2006/106Y10T29/49108
    • A method for producing a secondary cell having a flat wound electrode body that inhibits the bending of the electrode board caused by charging and discharging and inhibits resulting swelling of the cell and deterioration of cycle characteristics is provided. The method has the steps of: winding, with a winding core, a positive electrode board, a negative electrode board, and a separator provided between the positive and negative electrode boards, and fixing the winding end, thereby preparing an approximately cylindrical electrode body; after the step of preparing the electrode body, deforming the electrode body into a shape with an approximately oval cross section by pressing the approximately cylindrical electrode body from a direction perpendicular to the winding axis, and rotating the deformed electrode body in the winding direction, thereby relaxing the winding state; and after the relaxation steps, pressing the electrode body into the flat wound electrode body.
    • 提供一种具有扁平卷绕电极体的二次电池的制造方法,其抑制由充放电引起的电极板的弯曲,并且抑制电池的膨胀和循环特性的劣化。 该方法具有如下步骤:利用卷绕芯卷绕正极板,负极板和设置在正极板和负极板之间的隔板,并固定绕组端,从而制备大致圆柱形的电极体; 在准备电极体的步骤之后,通过从垂直于卷绕轴线的方向按压大致圆筒状的电极体,使电极体变形为具有大致椭圆形截面的形状,并使变形的电极体沿卷绕方向旋转,由此 放松缠绕状态; 并且在松弛步骤之后,将电极体压入扁平卷绕电极体中。
    • 10. 发明申请
    • Spiral electrode rectangular battery
    • 螺旋电极矩形电池
    • US20060222937A1
    • 2006-10-05
    • US11390075
    • 2006-03-28
    • Takuya MorimotoRyuji Ohshita
    • Takuya MorimotoRyuji Ohshita
    • H01M2/02H01M4/66H01M2/26H01M2/18
    • H01M10/0587H01M2/0207H01M2/22H01M2/30H01M4/661H01M4/70H01M10/0431
    • The spiral electrode rectangular battery has a first electrode plate and second electrode plate, which are the positive and negative electrodes, laminated together with a separator sandwiched in between, rolled into an electrode unit, and housed in an exterior casing. The electrode unit has flat surfaces on opposite sides, and edges on both sides of those flat surfaces are curved with a prescribed radius of curvature to form rounded edge regions. The first and second electrode plates are wound around flat surfaces and rounded edge regions in a continuous fashion, and the end of the outer-most electrode plate is disposed at a rounded edge region. In addition, corner cuts are provided to truncate corner regions formed between the edges at both sides and the end of the outer-most electrode plate.
    • 螺旋状电极矩形电池具有作为正极和负极的第一电极板和第二电极板,其间夹在其间的隔膜层压在一起,卷成电极单元,并容纳在外部壳体中。 电极单元在相对侧具有平坦表面,并且这些平坦表面两侧的边缘以规定的曲率半径弯曲以形成圆形边缘区域。 第一和第二电极板以连续的方式围绕平坦表面和圆形边缘区域缠绕,并且最外面的电极板的端部设置在圆形边缘区域。 此外,角切口被设置成在形成在两侧边缘和最外侧电极板的端部之间的角部区域。