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1. 发明授权

US09150891B2 Method for transforming stramenopiles 有权
标题翻译：转化层状砷的方法
公开(公告)号：US09150891B2
公开(公告)日：2015-10-06
申请号：US13497894
申请日：2010-09-24
申请人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
IPC分类号： C12P7/64 , C12P21/06 , C12N1/00 , C12N1/10 , C12N15/00 , C11C3/00 , C12N9/02 , C12N15/79 , C12N15/82
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了能够提高产生有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因，其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

2. 发明申请

US20120322116A1 METHOD FOR TRANSFORMING STRAMENOPILES 有权
标题翻译：改性方法
公开(公告)号：US20120322116A1
公开(公告)日：2012-12-20
申请号：US13497894
申请日：2010-09-24
申请人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
IPC分类号： C12P7/64 , C12N15/79
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了能够提高产生有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因，其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

3. 发明申请

US20120100550A1 METHOD FOR DETERMINING FATTY ACID SYNTHESIS PATHWAY OF MICROORGANISM, AND PCR PRIMER SET FOR USE IN THE METHOD 审中-公开
标题翻译：用于确定微生物的脂肪酸合成途径的方法，以及用于该方法的PCR引物组
公开(公告)号：US20120100550A1
公开(公告)日：2012-04-26
申请号：US13254794
申请日：2010-03-04
申请人： Naoki Nagano , Masahiro Hayashi , Keishi Sakaguchi , Makoto Ito , Yosuke Taoka , Yuji Okita
发明人： Naoki Nagano , Masahiro Hayashi , Keishi Sakaguchi , Makoto Ito , Yosuke Taoka , Yuji Okita
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for ?4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.
摘要翻译：公开了一种测定微生物的脂肪酸合成途径的方法。具体公开的是测定微生物的脂肪酸合成途径的方法，其特征在于制备脂肪酸合成相关酶基因的简并引物，并测定基因组基因中脂肪酸合成相关基因的存在或不存在使用简并引物从微生物中提取。作为脂肪酸合成相关酶基因的C20延伸酶基因的简并引物的序列是ATHGARTWYTKBRTITTYGTICA（SEQ ID NO：1）和TARTRISWRTACATIADIAMRTG（SEQ ID NO：2），并且用于Δ4的简并引物的序列 - 脱氢酶基因是GGNCAYCAYCMITAYACNAA（SEQ ID NO：3）和TCDATYTGRTGIBWIARNCC（SEQ ID NO：4）。该微生物属于Labyrinthulea类。还具体公开了用于确定方法的PCR引物组。该方法可用作筛选能够合成脂肪酸的微生物的方法。

4. 发明申请

US20130309772A1 METHOD FOR TRANSFORMATION OF STRAMENOPILE 有权
标题翻译： STRAMENOPILE转换方法
公开(公告)号：US20130309772A1
公开(公告)日：2013-11-21
申请号：US13877225
申请日：2011-09-30
申请人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
IPC分类号： C12N15/79 , C12N15/89
CPC分类号： C12N15/79 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/113 , C12N15/52 , C12N15/895 , C12N2310/11 , C12N2310/14 , C12P7/6409 , C12P7/6427 , C12P7/6472 , C12Y114/19001 , C12Y114/19006 , C12Y203/01119
摘要： To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
摘要翻译：提供通过以遗传工程方式破坏该层状生物体的基因或抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的前烧石生物的转化方法。 [解决方案]一种转基因生物的方法，其包括以基因工程方式破坏所述地精生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺破囊壶菌（Salisochytrium aureum），，鱼（Parietichytrium sarkarianum），玫瑰色胸，和Parietichytrium sp。并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

5. 发明授权

US09062315B2 Method for transformation of stramenopile 有权
标题翻译：地幔的转化方法
公开(公告)号：US09062315B2
公开(公告)日：2015-06-23
申请号：US13877225
申请日：2011-09-30
申请人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
IPC分类号： C12N15/79 , C12N15/89 , C12N9/02 , C12N9/10 , C12P7/64 , C12N15/52
CPC分类号： C12N15/79 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/113 , C12N15/52 , C12N15/895 , C12N2310/11 , C12N2310/14 , C12P7/6409 , C12P7/6427 , C12P7/6472 , C12Y114/19001 , C12Y114/19006 , C12Y203/01119
摘要： To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
摘要翻译：提供通过以遗传工程方式破坏该层状生物体的基因或抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的前烧石生物的转化方法。 [解决方案]一种转基因生物的方法，其包括以基因工程方式破坏所述地精生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺破囊壶菌（Salisochytrium aureum），，鱼（Parietichytrium sarkarianum），玫瑰色胸，和Parietichytrium sp。并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

6. 发明授权

US08349595B2 Method for increasing the content of docosahexaenoic acid in fat-containing materials or in fats and oils 失效
标题翻译：增加含脂肪物质或脂肪和油中二十二碳六烯酸含量的方法
公开(公告)号：US08349595B2
公开(公告)日：2013-01-08
申请号：US12524647
申请日：2008-01-25
申请人： Masahiro Hayashi , Sanae Nakajima , Naoki Nagano , Yosuke Taoka
发明人： Masahiro Hayashi , Sanae Nakajima , Naoki Nagano , Yosuke Taoka
IPC分类号： C12P7/64 , C12N1/12 , C07C57/03
CPC分类号： C12P7/6427 , C11C3/00 , C12N1/14 , C12P7/6472
摘要： [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased.[MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method. Particularly, the process for producing highly unsaturated fatty acids, the method for enhancing the productivity of highly unsaturated fatty acids, and the stramenopile having the enhanced productivity of highly unsaturated fatty acids, wherein the stramenopile is a microorganism classified into Labyrinthulea.
摘要翻译： [问题]提供其中二十二碳六烯酸含量增加的脂肪和油。解决问题的手段一种高不饱和脂肪酸的制造方法，其特征在于，在含有脂肪酸去饱和酶抑制剂的培养基中培养能够生产高度不饱和脂肪酸的前列腺素; 其中通过相关方法生产的高度不饱和脂肪酸，特别是二十二碳六烯酸的含量增加的脂肪和油。一种增强层状高分子量不饱和脂肪酸的生产率的方法，包括在含有脂肪酸去饱和酶抑制剂的培养基中培养前列腺素; 具有通过相关方法生成的高度不饱和脂肪酸的生产率提高的前列腺素。特别是生产高不饱和脂肪酸的方法，提高高不饱和脂肪酸的生产率的方法以及具有提高的高度不饱和脂肪酸的生产率的前列腺素，其中所述地釉菌是分类为迷迭虫的微生物。

7. 发明申请

US20100099901A1 METHOD FOR INCREASING THE CONTENT OF DOCOSAHEXAENOIC ACID IN FAT-CONTAINING MATERIALS OR IN FATS AND OILS 失效
标题翻译：在含脂肪物质或脂肪和油中增加含二氧化碳的含量的方法
公开(公告)号：US20100099901A1
公开(公告)日：2010-04-22
申请号：US12524647
申请日：2008-01-25
申请人： Masahiro Hayashi , Sanae Nakajima , Naoki Nagano , Yosuke Taoka
发明人： Masahiro Hayashi , Sanae Nakajima , Naoki Nagano , Yosuke Taoka
IPC分类号： C07C57/03 , C12P7/64 , C12N1/12
CPC分类号： C12P7/6427 , C11C3/00 , C12N1/14 , C12P7/6472
摘要： [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased.[MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method. Particularly, the process for producing highly unsaturated fatty acids, the method for enhancing the productivity of highly unsaturated fatty acids, and the stramenopile having the enhanced productivity of highly unsaturated fatty acids, wherein the stramenopile is a microorganism classified into Labyrinthulea.
摘要翻译： [问题]提供其中二十二碳六烯酸含量增加的脂肪和油。解决问题的手段一种高不饱和脂肪酸的制造方法，其特征在于，在含有脂肪酸去饱和酶抑制剂的培养基中培养能够生产高度不饱和脂肪酸的前列腺素; 其中通过相关方法生产的高度不饱和脂肪酸，特别是二十二碳六烯酸的含量增加的脂肪和油。一种增强层状高分子量不饱和脂肪酸的生产率的方法，包括在含有脂肪酸去饱和酶抑制剂的培养基中培养前列腺素; 具有通过相关方法生成的高度不饱和脂肪酸的生产率提高的前列腺素。特别是生产高不饱和脂肪酸的方法，提高高不饱和脂肪酸的生产率的方法以及具有提高的高度不饱和脂肪酸的生产率的前列腺素，其中所述地釉菌是分类为迷迭虫的微生物。

8. 发明申请

US20060033957A1 Data providing system, data providing apparatus and method, data acquisition system and method, and program storage medium 失效
公开(公告)号：US20060033957A1
公开(公告)日：2006-02-16
申请号：US11252170
申请日：2005-10-17
申请人： Jun Kaneko , Naoki Nagano , Satoru Kamoto , Katsushi Fujii , Mayumi Shinoda , Atsushi Fuse , Michinari Kohno
发明人： Jun Kaneko , Naoki Nagano , Satoru Kamoto , Katsushi Fujii , Mayumi Shinoda , Atsushi Fuse , Michinari Kohno
IPC分类号： G06F3/12
CPC分类号： G06Q30/02 , G06Q30/0272 , G06Q30/0273 , G06Q30/0277
摘要： The present invention is intended to provide a data providing system including a data providing apparatus having provision image data generating means for generating provision image data of a provision image which is reusable by inlaying a predetermined advertisement image into a predetermined template image into which a predetermined user image can be inlaid as superimposed upon the predetermined advertisement image and providing-side transmitting means for transmitting the provision image data via a network. The data providing system also includes a data acquisition apparatus having acquiring-side receiving means for receiving the provision image data from the data providing apparatus via the network, transmission image data generating means for generating transmission image data of a transmission image for displaying the provision image based on the provision image data onto a predetermined display means, inlaying the predetermined user image as superimposed upon the predetermined advertisement image into the template image of the provision image, and displaying the predetermined user image together with the template image in preference to the predetermined advertisement image until the predetermined user image is detached from the template image, and acquiring-side transmitting means for transmitting the transmission image data to a predetermined destination via the network.

9. 发明授权

US06522361B2 Electronic apparatus having the function of displaying the battery residual quantity and method for displaying the battery residual quantity 失效
标题翻译：具有显示电池剩余量的功能的电子设备和显示电池剩余量的方法
公开(公告)号：US06522361B2
公开(公告)日：2003-02-18
申请号：US08810989
申请日：1997-02-27
申请人： Yoshinari Higuchi , Toshio Koyama , Naoki Nagano , Yoichi Tsusue
发明人： Yoshinari Higuchi , Toshio Koyama , Naoki Nagano , Yoichi Tsusue
IPC分类号： H04N5225
CPC分类号： G01R31/3613 , G01R31/3648
摘要： An electronic equipment having the function of displaying the battery residual quantity for displaying the usable residual time of a battery pack used as a power source of the electronic equipment. The equipment is designed to cope with different types or future version of the battery cell. The residual usable time of the battery can be known with improved display accuracy. The video camera 60 carries a battery pack 1 outputting at least the information on the residual battery capacity, charging/discharging current detection information and the battery cell voltage detection information. The video camera 60 includes a micro-computer 63 having a communication circuit 65 for receiving the above information from the battery pack 1, a calculation circuit 66 for calculating the current residual battery capacity based on the information from the battery pack 1 received by the communication circuit 65, and a display control circuit 67, and a display device 64 fed with a display signal corresponding to the results of calculations by the calculation circuit 66 of the micro-computer 63 for displaying the residual battery capacity based on the display signal.
摘要翻译：具有显示电池剩余量的功能的电子设备，用于显示用作电子设备的电源的电池组的可用剩余时间。该设备旨在应对不同类型或未来版本的电池。电池的剩余可用时间可以通过改进的显示精度而已知。摄像机60携带至少输出关于剩余电池容量，充电/放电电流检测信息和电池单元电压检测信息的信息的电池组1。视频摄像机60包括具有用于从电池组1接收上述信息的通信电路65的微型计算机63，用于根据通信所接收的来自电池组1的信息计算当前剩余电池容量的计算电路66 电路65和显示控制电路67以及显示装置64，显示装置64输出与微计算机63的计算电路66相对应的显示信号，用于显示基于显示信号的剩余电池容量。

10. 发明授权

US6016139A Motion picture reproducing and recording/reproduction apparatus 失效
标题翻译：运动图像再现和记录/再现装置
公开(公告)号：US6016139A
公开(公告)日：2000-01-18
申请号：US932788
申请日：1997-09-18
申请人： Hideo Terasawa , Yoshio Kondo , Naoki Nagano , Kunihito Sawai
发明人： Hideo Terasawa , Yoshio Kondo , Naoki Nagano , Kunihito Sawai
IPC分类号： G11B27/10 , G11B27/11 , G11B27/13 , G11B27/30 , G11B27/32 , G11B27/34 , G09G5/08 , H04N5/44
CPC分类号： G11B27/34 , G11B27/102 , G11B27/107 , G11B27/11 , G11B27/13 , G11B27/3027 , G11B27/326 , G11B27/328 , G11B2220/20 , G11B2220/2525 , G11B2220/65 , G11B2220/90
摘要： In a motion picture reproducing apparatus, or a motion picture recording and reproducing apparatus, an index picture storage means stores a plurality of index pictures extracted from a series of motion pictures in time sequence at a assigned extraction timing in respective storage positions in a motion picture storage medium by associating each index picture with a different storage position. An index picture display means arranges and displays the index pictures read from the index picture storage means on the display screen in time sequence. Selective means select a desired target reproduction picture to be reproduced, and reproduction position control means allow movement to a recording position corresponding to the selected target reproduction position to initiate a reproducing operation, thus allowing search conditions to be visually and intuitively determined when searching stored data on the motion picture storage medium, and providing substantially improved user-friendliness.
摘要翻译：在运动图像再现装置或运动图像记录和再现装置中，索引图像存储装置在运动图像的各个存储位置中以分配的提取定时在时间序列中存储从一系列运动图像提取的多个索引图像通过将每个索引图片与不同的存储位置相关联来存储介质。索引图像显示装置按时间顺序在显示屏幕上排列并显示从索引图像存储装置读取的索引图像。选择性装置选择要再现的所需目标再现图像，再现位置控制装置允许移动到与所选择的目标再现位置对应的记录位置，以开始再现操作，从而允许在搜索存储的数据时视觉和直观地确定搜索条件并且提供大大改善的用户友好性。

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