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1. 发明授权

US09068155B2 Array of micromolded structures for sorting adherent cells 有权
标题翻译：用于分类贴壁细胞的微复合结构阵列
公开(公告)号：US09068155B2
公开(公告)日：2015-06-30
申请号：US13513310
申请日：2012-06-01
申请人： Nancy Allbritton , Christopher Sims , Yuli Wang , Pavak Kirit Shah
发明人： Nancy Allbritton , Christopher Sims , Yuli Wang , Pavak Kirit Shah
IPC分类号： C40B50/06 , C40B60/14 , C12M1/32 , C12M1/12
CPC分类号： C12M23/12 , B01J19/0046 , B01J2219/00452 , B01J2219/00743 , C12M23/20 , C12M23/22 , C12M25/04 , C12M25/16 , C12M47/02 , C12M47/04
摘要： An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).
摘要翻译：用于收集或培养细胞或细胞集落的装置包括：由柔性弹性聚合物材料形成且在其中形成有多个孔的共同基底; 以及可释放地连接到所述公共衬底的多个刚性电池载体，所述载体以阵列的形式布置，并且每个载体弹性地容纳在其中一个孔中。使用这种装置收集或培养细胞或细胞集落的方法是通过在装置上沉积携带细胞的液体培养基进行的，以使所述细胞沉降或粘附到所述载体上; 然后（c）通过从其被接收的空腔（例如通过用探针推动）逐渐地向每个载体施加释放能量，从而释放至少一个具有所述细胞的选定载体。

2. 发明申请

US20130065795A1 ARRAY OF MICROMOLDED STRUCTURES FOR SORTING ADHERENT CELLS 有权
公开(公告)号：US20130065795A1
公开(公告)日：2013-03-14
申请号：US13513310
申请日：2012-06-01
申请人： Nancy Allbritton , Christopher Sims , Yuli Wang , Pavak Kirit Shah
发明人： Nancy Allbritton , Christopher Sims , Yuli Wang , Pavak Kirit Shah
IPC分类号： C40B50/06 , C40B60/14
CPC分类号： C12M23/12 , B01J19/0046 , B01J2219/00452 , B01J2219/00743 , C12M23/20 , C12M23/22 , C12M25/04 , C12M25/16 , C12M47/02 , C12M47/04
摘要： An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

3. 发明申请

US20070238122A1 SYSTEMS AND METHODS FOR EFFICIENT COLLECTION OF SINGLE CELLS AND COLONIES OF CELLS AND FAST GENERATION OF STABLE TRANSFECTANTS 有权
标题翻译：有效收集单细胞和细胞群的系统和方法以及快速生成稳定转录因子
公开(公告)号：US20070238122A1
公开(公告)日：2007-10-11
申请号：US11733053
申请日：2007-04-09
申请人： Nancy Allbritton , Christopher Sims , Yuli Wang , Mark Bachman , Guann-Pyng Li , Eric Stanbridge
发明人： Nancy Allbritton , Christopher Sims , Yuli Wang , Mark Bachman , Guann-Pyng Li , Eric Stanbridge
IPC分类号： C12Q1/68 , C12N1/20
CPC分类号： G01N1/286 , B01L3/5085 , B01L2200/0668 , B01L2300/0829 , C12M23/12 , C12M47/04 , G01N2001/2886
摘要： A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses. Use of the plate advantageously enables the selection and sorting of cells based on dynamic phenomena and the rapid establishment of stable tranfectants.
摘要翻译：制造的板可使细胞，微生物，蛋白质，DNA，生物分子和其他生物培养基的样品定位在板上的特定位置或位点，以便对样品进行可寻址分析。优选地，一些或所有的部位由可移除的材料或托盘构建，使得感兴趣的样品的子集可以容易地与板分离以用于进一步的处理或分析。该板可以包含增强或促进样品的附着和/或功能的结构或化学处理，并且促进或辅助其分析。板的使用有利于基于动态现象和快速建立稳定的转染剂来选择和分选细胞。

4. 发明申请

US20070128716A1 MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS 有权
标题翻译：用于绘制生物和非生物材料的微孔板
公开(公告)号：US20070128716A1
公开(公告)日：2007-06-07
申请号：US11539695
申请日：2006-10-09
申请人： Yuli Wang , Mark Bachman , Christopher Sims , Guann-Pyng Li , Nancy Allbritton
发明人： Yuli Wang , Mark Bachman , Christopher Sims , Guann-Pyng Li , Nancy Allbritton
IPC分类号： C12M1/22
CPC分类号： B01L3/5085 , B01L3/5088 , B01L2200/0642 , B01L2200/12 , B01L2300/0819 , C12M25/06 , C12M29/20
摘要： Systems and methods are provided for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiment of the present invention include a method for placing materials on the plate at pre-determined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate with coatings at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micro-mechanical structures—where the structures may be formed from living or non-living material, tissue or non-tissue, organic or inorganic, and the like.
摘要翻译：提供了系统和方法，用于在板上的特定位置图案化生物和非生物材料，以及生长三维结构。优选实施例包括具有区域的板，当板被浸没在液体中时，其具有通常以气泡形式捕获气体的区域。本发明的其它实施方案包括通过使用捕获的气体将材料放置在预定位置处的材料的方法，以防止材料在不需要的区域收集。该板对于在特定位置（例如阵列）上的电镀细胞和组织具有很大的用途。所公开的方法还可以用于在具有特定位置的涂层涂覆板的表面以用于图案化涂布应用，并且建立材料以产生三维结构，包括微机械结构，其中结构可以由活的或非 - 生物材料，组织或非组织，有机或无机物等。

5. 发明申请

US20070292312A1 METHOD OF MANUFACTURE OF A PLATE OF RELEASABLE ELEMENTS AND ITS ASSEMBLY INTO A CASSETTE 审中-公开
标题翻译：可释放元素板的制造方法及其组装于CASSETTE
公开(公告)号：US20070292312A1
公开(公告)日：2007-12-20
申请号：US11742006
申请日：2007-04-30
申请人： Mark Bachman , Yuli Wang , Christopher Sims , Nancy Allbritton , Guann-Pyng Li
发明人： Mark Bachman , Yuli Wang , Christopher Sims , Nancy Allbritton , Guann-Pyng Li
IPC分类号： B01L3/02 , G01N35/00 , G03F7/038
CPC分类号： B01L3/502707 , B01L3/5085 , B01L3/5088 , B01L2200/0647 , B01L2300/0636 , B01L2300/0822 , B01L2300/0829 , B01L2300/089 , B01L2400/0454 , G01N2035/00158
摘要： A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses.
摘要翻译：制造的板可使细胞，微生物，蛋白质，DNA，生物分子和其他生物培养基的样品定位在板上的特定位置或位点，以便对样品进行可寻址分析。优选地，一些或所有的部位由可移除的材料或托盘构建，使得感兴趣的样品的子集可以容易地与板分离以用于进一步的处理或分析。该板可以包含增强或促进样品的附着和/或功能的结构或化学处理，并且促进或辅助其分析。

6. 发明申请

US20130066031A1 MICROMOLDING OF POLYSTYRENE BY SOFT LITHOGRAPHY 审中-公开
标题翻译：通过软平台对聚苯乙烯进行微型化
公开(公告)号：US20130066031A1
公开(公告)日：2013-03-14
申请号：US13596603
申请日：2012-08-28
申请人： Nancy Allbritton , Christopher Sims , Yuli Wang
发明人： Nancy Allbritton , Christopher Sims , Yuli Wang
IPC分类号： B28B11/08 , C08F120/10 , C08G63/08 , C08F112/08
CPC分类号： B01L3/502707 , B01L3/5085 , B01L2200/0689 , B01L2200/12 , B01L2300/0819 , B01L2300/0893 , B29C65/02 , B29C66/53461 , B29C66/71 , B29C66/8122 , B29C66/91945 , B29L2031/756 , B82Y10/00 , B82Y40/00 , G03F7/0002 , B29K2025/06 , B29K2909/08
摘要： A method of making such is carried out by (a) providing a reverse template for said article, said template comprising an elastomeric polymer such as PDMS; (b) providing a solution comprising a polymer dissolved in a first solvent; wherein said polymer is selected from the group consisting of polystyrene, poly(methyl methacrylate), epoxy, and aliphatic polyesters; and wherein said solvent comprises a lactone; (c) depositing said solution on said template; (e) removing said solvent from said template to form said article from said polymer on said template; and then (f) separating said template from said article.
摘要翻译：通过（a）为所述制品提供反向模板来进行制造方法，所述模板包括弹性聚合物如PDMS; （b）提供包含溶解在第一溶剂中的聚合物的溶液; 其中所述聚合物选自聚苯乙烯，聚（甲基丙烯酸甲酯），环氧树脂和脂族聚酯; 并且其中所述溶剂包含内酯; （c）将所述溶液沉积在所述模板上; （e）从所述模板中除去所述溶剂以在所述模板上从所述聚合物形成所述制品; 然后（f）从所述制品中分离所述模板。

7. 发明申请

US20110294208A1 METHOD AND DEVICE FOR CELL SELECTION AND COLLECTION IN AN ISOLATED CULTURING ENVIRONMENT 审中-公开
标题翻译：用于分离培养环境中细胞选择和收集的方法和装置
公开(公告)号：US20110294208A1
公开(公告)日：2011-12-01
申请号：US13145917
申请日：2010-02-11
申请人： Nancy Allbritton , Christopher Sims , Wei Xu
发明人： Nancy Allbritton , Christopher Sims , Wei Xu
IPC分类号： C12N5/16 , C12M1/00 , C12N5/071 , C12M1/12 , C12N1/20 , C12N1/14 , C12N1/16 , C12M3/00 , C12N5/075
CPC分类号： C12M25/04 , C12M23/16
摘要： An apparatus for collecting or culturing cells or cell colonies has a common substrate and a plurality of cell carriers releasably connected to the common substrate. The carriers are arranged in the form of an array. The invention employs microcups as the cell carriers. The substrate is preferably free of barriers between the microcups, and in some embodiments the microcups have porous walls. Methods of using the apparatus are also described.
摘要翻译：用于收集或培养细胞或细胞集落的装置具有公共基底和可释放地连接到共同基底的多个细胞载体。载体以阵列的形式排列。本发明采用微型杯作为细胞载体。基底优选在微杯之间不存在阻挡层，并且在一些实施例中，微型杯具有多孔壁。还描述了使用该装置的方法。

8. 发明授权

US07236888B2 Method to measure the activation state of signaling pathways in cells 有权
标题翻译：测量细胞中信号通路活化状态的方法
公开(公告)号：US07236888B2
公开(公告)日：2007-06-26
申请号：US09990413
申请日：2001-11-21
申请人： Nancy Allbritton , Christopher Sims
发明人： Nancy Allbritton , Christopher Sims
IPC分类号： G06F19/00 , C12Q1/25
CPC分类号： G01N15/1475 , B01L3/5027 , G01N1/02 , G01N33/5005 , G01N33/6803
摘要： The activity of multiple proteins in a single living cell, portion of a cell or in a group of cells is simultaneously measured by introducing reporter molecules into the cell(s) or a portion thereof, chemically modifying the reporter(s) by the enzyme of interest, terminating the modification reactions, removing the reporter(s) and modified reporter(s), and determining the amount of enzyme activity present by measuring or comparing the amount of reporter(s) and modified reporter(s) present. By performing a series of experiments at different time points, conditions, and varieties of cell types, a database is developed for molecular cellular mechanisms in health and disease states. By exposing cells to a variety of compounds data for drug development and screening is provided.
摘要翻译：通过将报告分子引入细胞或其一部分，通过将酶的一个或多个酶的化学修饰报告物同时测量多个蛋白质在单个活细胞，细胞部分或一组细胞中的活性终止修饰反应，除去报告基团和修饰的报告物，以及通过测量或比较存在的报告基团和修饰的报告子的量来测定存在的酶活性的量。通过在不同的时间点，条件和细胞类型的品种进行一系列实验，为健康和疾病状态的分子细胞机制开发了数据库。通过将细胞暴露于各种化合物，提供用于药物开发和筛选的数据。

9. 发明申请

US20050237480A1 Chemical modifications to polymer surfaces and the application of polymer grafting to biomaterials 审中-公开
公开(公告)号：US20050237480A1
公开(公告)日：2005-10-27
申请号：US10511788
申请日：2003-05-13
申请人： Nancy Allbritton , Christopher Sims , Guann-Pyng Li , Mark Bachman , Shuwen Hu , Xueqin Ren
发明人： Nancy Allbritton , Christopher Sims , Guann-Pyng Li , Mark Bachman , Shuwen Hu , Xueqin Ren
IPC分类号： C08J7/18 , G01N27/447 , G02B1/04 , G02C7/04
CPC分类号： C08J7/18 , B01J2219/00605 , B01J2219/0061 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , G01N27/44704 , G01N27/44752 , G02B1/043
摘要： Polymer-based biomaterials are popular due to ease of fabrication and low costs. However, many polymer substrates have undesirable surface properties. The invention provides a procedure to covalently apply a graft polymer to the surface of a polymer substrate by ultraviolet graft polymerization. The graft polymer is formed from monomers such as PEG, AA, monomethoxy acrylate PEG, HEMA, or DMA. Also, mixed monomers may be used to create the graft and the surface properties of the graft may be tailored for different properties, including hydrophobicity, friction coefficient, electroosmotic mobilities and electrophoretic separations. The invention has particular utility in tailoring surface chemistries in ocular lenses and polymer microdevices. I. II. R:—OHAcrylic Acid(AA) —NH2Acrylamide (AM) —N(CH3)2Dimethylacrylamide (DMA) —OCH2CH2OH2-Hydroxyethylacrylate (HEA) —O(CH2CH2O)nCH3PEG monomethyoxylacrylate (PEG)

10. 发明申请

US20110281743A1 SYSTEMS AND METHODS FOR ISOLATING CELLS IN CELL COLONIES IN CULTURE 审中-公开
标题翻译：用于在细胞培养中分离细胞的系统和方法
公开(公告)号：US20110281743A1
公开(公告)日：2011-11-17
申请号：US13130345
申请日：2009-12-10
申请人： Nancy Allbritton , Christopher Sims , Wei Xu , Hamed Shadpour
发明人： Nancy Allbritton , Christopher Sims , Wei Xu , Hamed Shadpour
IPC分类号： C40B30/00 , C12Q1/68 , H01J49/26 , C40B60/12 , H01J37/26 , C12Q1/02 , G01N33/53
CPC分类号： C12Q1/24 , B01L3/5025 , B01L3/5085 , B01L2200/0668 , B01L2300/0609 , B01L2300/0819
摘要： Selecting and propagating a cell colony of interest from among a plurality of cell colonies carried on a common substrate in culture is carried out by: (a) selecting a cell colony of interest from among the plurality of cell colonies; (b) isolating a cell subset from the cell colony of interest; (c) analyzing (for example, by a destructive analysis) the cell subset isolated from the cell colony of interest to confirm the presence or absence of a desired feature therein; and then (d) propagating the cell colony of interest when the desired feature is present in the cell subset. A micropallet apparatus may include: (a) a substrate; (b) a plurality of discrete arrays formed on the substrate, each of the arrays comprising a plurality of releasable pallets, and (c) a plurality of gap forming regions, wherein the gap forming regions surround the pallets and separate the pallets from one another.
摘要翻译：通过以下步骤进行选择和繁殖培养中携带在共同底物上的多个细胞集落中的感兴趣的细胞集落：（a）从多个细胞集落中选择感兴趣的细胞集落; （b）从感兴趣的细胞集落分离细胞亚群; （c）分析（例如，通过破坏性分析）从感兴趣的细胞集落分离的细胞亚群以确认其中存在或不存在所需特征; 然后（d）当所需特征存在于细胞子集中时，传播感兴趣的细胞集落。微孔装置可以包括：（a）基底; （b）形成在所述基板上的多个分立阵列，每个所述阵列包括多个可释放的托盘，以及（c）多个间隙形成区域，其中所述间隙形成区域围绕所述托盘并将所述托盘彼此分离。

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