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    • 10. 发明申请
    • METHOD AND COMPOSITIONS FOR ENHANCING AN X143 POLYPEPTIDE HAVING RETROGRADED STARCH DEGRADING ACTIVITY AND USES THEREOF
    • 用于增强具有改性淀粉降解活性的X143多肽的方法和组合物及其用途
    • WO2014197705A1
    • 2014-12-11
    • PCT/US2014/041113
    • 2014-06-05
    • NOVOZYMES A/SSOONG, Chee-Leong
    • SOONG, Chee-LeongHARRIS, PaulKIEMER, Lars
    • C12N9/22C12P7/06
    • C12N9/2411C12P7/06C12P19/14C12Y101/99018C12Y302/00Y02E50/17
    • The present invention relates to a composition comprising: (a) an X143 polypeptide having retrograded starch degrading activity and (b) a sulfur-containing compound, and/or a heterocyclic compound (reducing agent), wherein the combination of the X143 polypeptide having retrograded starch degrading activity and a sulfur-containing compound and/or a heterocyclic compound (reducing agent), and/or a cellobiose dehydrogenase, enhances degradation of retrograded starch material by the X143 polypeptide. The present invention further relates to a method for degrading or converting a retrograded starch material, comprising: treating the retrograded starch material with an enzyme composition in the presence of an X143 polypeptide having retrograded starch degrading activity and a sulfur-containing compound and/or a heterocyclic compound (reducing agent), and/or a cellobiose dehydrogenase, wherein the combination of the polypeptide having retrograded starch degrading activity and the sulfur-containing compound and/or the heterocyclic compound (reducing agent), and/or the cellobiose dehydrogenase enhances degradation of the retrograded starch material by the enzyme composition.
    • 本发明涉及一种组合物,其包含:(a)具有退化淀粉降解活性的X143多肽和(b)含硫化合物和/或杂环化合物(还原剂),其中所述具有退化的X143多肽的组合 淀粉降解活性和含硫化合物和/或杂环化合物(还原剂)和/或纤维二糖脱氢酶增强了X143多肽对退化淀粉材料的降解。 本发明还涉及一种降解或转化退化淀粉材料的方法,包括:在具有退化的淀粉降解活性的X143多肽和含硫化合物和/或其盐的存在下,用酶组合物处理退化的淀粉材料 杂环化合物(还原剂)和/或纤维二糖脱氢酶,其中具有退化淀粉降解活性的多肽与含硫化合物和/或杂环化合物(还原剂)和/或纤维二糖脱氢酶的组合增强降解 的淀粉原料由酶组成。