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    • 4. 发明申请
    • FUNGAL PEPC INHIBITOR
    • 真菌PEPC抑制剂
    • WO2009071530A1
    • 2009-06-11
    • PCT/EP2008/066605
    • 2008-12-02
    • NOVOZYMES A/SLEHMBECK, JanUDAGAWA, Hiroaki
    • LEHMBECK, JanUDAGAWA, Hiroaki
    • C07K14/38C12N15/80
    • C07K14/38C12N15/80
    • The present invention relates to an isolated polypeptide having PepC inhibitory activityas well as to a methodfor producing a heterologous polypeptide of interest in an Aspergillus host cell comprising: (a) cultivating the Aspergillushost cell comprising a first and a second nucleic acid sequences under conditions conducive for the expression of the polypeptides encoded by the said first and second nucleic acid sequences, and wherein the first nucleic acid sequence encodes a heterologous polypeptide of interest and the second nucleic acid encodes the inhibitor polypeptide of the invention, and wherein the inhibitor polypeptide is expressed from a recombinant nucleic acid construct resulting in an increased level of the inhibitor polypeptide compared to an Aspergillushost cell not comprising the recombinant nucleic acid construct; and (b) recovering the heterologous polypeptide.
    • 本发明涉及具有PepC抑制活性的分离的多肽以及在曲霉属宿主细胞中产生感兴趣的异源多肽的方法,包括:(a)在有利于(a)的条件下培养包含第一和第二核酸序列的曲霉菌细胞 由所述第一和第二核酸序列编码的多肽的表达,并且其中所述第一核酸序列编码感兴趣的异源多肽,所述第二核酸编码本发明的抑制剂多肽,并且其中所述抑制剂多肽由 重组核酸构建体与不包含重组核酸构建体的曲霉属细胞相比导致抑制剂多肽的水平增加; 和(b)回收异源多肽。