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    • 5. 发明申请
    • XYLANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    • XYLANASE变体和编码它们的多核苷酸
    • WO2015200659A1
    • 2015-12-30
    • PCT/US2015/037732
    • 2015-06-25
    • NOVOZYMES A/SSCOTT, Brian R.
    • SCOTT, Brian R.WOGULIS, MarkPEDERSEN, SvenLAVIGNE, James
    • C12N9/24
    • C12P7/10C12N9/2402C12N9/248C12N9/2482C12N15/52C12N15/8257C12Y302/01008C12Y302/01037
    • The present invention relates to xylanase variants, comprising an alteration at least at one position corresponding to position 87 of the polypeptide of SEQ ID NO: 3, wherein the variant has xylanase activity and has increased xylanase inhibitor tolerance compared to the xylanase of SEQ ID NO: 3; and i) wherein the variant has at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3; or ii) wherein the number of alterations is 1-20, e.g., 1-10 such as 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 alterations. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    • 本发明涉及木聚糖酶变体,其包含与SEQ ID NO:3的多肽的位置87相对应的至少一个位置的改变,其中所述变体具有木聚糖酶活性,并且与SEQ ID NO:3的木聚糖酶相比具有增加的木聚糖酶抑制剂耐受性 :3; 和i)其中所述变体具有至少75%,至少80%,至少85%,至少90%,至少95%,至少96%,至少97%,至少98%或至少 99%,但小于100%与SEQ ID NO:3的多肽的序列同一性; 或ii)其中改变的数目是1-20,例如1-10,例如1,2,3,4,5,6,7,8,9或10个改变。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体,载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。