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    • 1. 发明申请
    • XYLANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    • XYLANASE变体和编码它们的多核苷酸
    • WO2015200659A1
    • 2015-12-30
    • PCT/US2015/037732
    • 2015-06-25
    • NOVOZYMES A/SSCOTT, Brian R.
    • SCOTT, Brian R.WOGULIS, MarkPEDERSEN, SvenLAVIGNE, James
    • C12N9/24
    • C12P7/10C12N9/2402C12N9/248C12N9/2482C12N15/52C12N15/8257C12Y302/01008C12Y302/01037
    • The present invention relates to xylanase variants, comprising an alteration at least at one position corresponding to position 87 of the polypeptide of SEQ ID NO: 3, wherein the variant has xylanase activity and has increased xylanase inhibitor tolerance compared to the xylanase of SEQ ID NO: 3; and i) wherein the variant has at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3; or ii) wherein the number of alterations is 1-20, e.g., 1-10 such as 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 alterations. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    • 本发明涉及木聚糖酶变体,其包含与SEQ ID NO:3的多肽的位置87相对应的至少一个位置的改变,其中所述变体具有木聚糖酶活性,并且与SEQ ID NO:3的木聚糖酶相比具有增加的木聚糖酶抑制剂耐受性 :3; 和i)其中所述变体具有至少75%,至少80%,至少85%,至少90%,至少95%,至少96%,至少97%,至少98%或至少 99%,但小于100%与SEQ ID NO:3的多肽的序列同一性; 或ii)其中改变的数目是1-20,例如1-10,例如1,2,3,4,5,6,7,8,9或10个改变。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体,载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。
    • 7. 发明申请
    • PROCESS FOR SIMULTANEOUS SACCHARFICATION AND FERMENTATION OF WHEY PERMEATE
    • 同时渗透和发酵白藜芦醇的方法
    • WO2014184189A2
    • 2014-11-20
    • PCT/EP2014/059756
    • 2014-05-13
    • NOVOZYMES A/S
    • PEDERSEN, Sven
    • C12P7/08
    • C12P7/14C12P7/08
    • A process for producing ethanol from lactose containing substrates, comprising simultaneously saccharifying the substrate to produce monosaccharide and fermenting the monosaccharide to produce ethanol at a pH from 3.5 - 5.5, using a fermenting organism, wherein saccharification is carried out in the presence of a lactase, and wherein the fermenting organism is a Saccharomyces sp., and the ratio between the incubation time required for obtaining at least 90% hydrolysis of the lactose present in the substrate (t- 1 ) and the total fermentation time (t 2 ) is in the range of 0.1 to 1, and the Saccharomyces sp. is added in amounts that will result in an ethanol yield of at least 70 % w/w of the theoretical ethanol yield from lactose by the end of fermentation.
    • 一种由含乳糖的底物生产乙醇的方法,其包括同时使底物糖化以制备单糖并使用发酵生物在3.5-5.5的pH下发酵所述单糖以产生乙醇,其中糖化在乳糖酶的存在下进行, 并且其中所述发酵生物是酵母属,并且获得基质(t-1)中存在的乳糖至少90%水解所需的孵育时间与总发酵时间(t2)之间的比例在该范围内 为0.1至1,和酵母属 加入量将导致在发酵结束时从乳糖产生的理论乙醇产率至少为70%w / w的乙醇产量。