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1. 发明申请

WO2013088259A3 METHODS OF PURIFYING ANTIBODIES 审中-公开
标题翻译：纯化抗体的方法
公开(公告)号：WO2013088259A3
公开(公告)日：2013-11-14
申请号：PCT/IB2012003028
申请日：2012-10-19
申请人： NOVIMMUNE SA , ELSON GREG , FOUQUE NICOLAS , DEPOISIER JEAN-FRANCOIS , FISCHER NICOLAS , MAGISTRELLI GIOVANNI
发明人： ELSON GREG , FOUQUE NICOLAS , DEPOISIER JEAN-FRANCOIS , FISCHER NICOLAS , MAGISTRELLI GIOVANNI
IPC分类号： C07K16/00 , C07K16/46
CPC分类号： C07K16/00 , C07K16/248 , C07K16/249 , C07K16/2866 , C07K16/468 , C07K2317/32 , C07K2317/52
摘要： The invention provides methods of purifying antibodies using various antibody- specific purification media to rapidly and efficiently separate mixtures of antibodies, antibody fragments and/or antibody components to isolate a desired antibody product from the mixture. The invention relates to the purification of bispecific monoclonal antibodies carrying a different specificity for each binding site of the immunoglobulin molecule, e.g., antibodies composed of a single heavy chain and two different light chains, one containing Kappa constant domain and the other a Lambda constant domain, including antibodies of different specificities that share a common heavy chain. The invention also provides the methods of efficiently purifying intact antibodies by separating the intact antibody from non-intact antibodies including free light chains.
摘要翻译：本发明提供了使用各种抗体特异性纯化介质来纯化抗体以快速且有效地分离抗体，抗体片段和/或抗体组分的混合物以从混合物中分离出所需抗体产物的方法。本发明涉及纯化对免疫球蛋白分子的每个结合位点具有不同特异性的双特异性单克隆抗体，例如由单一重链和两个不同轻链组成的抗体，一个含有κ恒定结构域，另一个含有λ恒定结构域包括具有共同重链的不同特异性的抗体。本发明还提供了通过将完整抗体与包括游离轻链的非完整抗体分开来有效纯化完整抗体的方法。

2. 发明申请

WO2012023053A3 METHODS FOR THE GENERATION OF MULTISPECIFIC AND MULTIVALENT ANTIBODIES 审中-公开
标题翻译：用于产生多重和多重抗体的方法
公开(公告)号：WO2012023053A3
公开(公告)日：2012-05-24
申请号：PCT/IB2011002664
申请日：2011-08-16
申请人： NOVIMMUNE SA , FISCHER NICOLAS , MAGISTRELLI GIOVANNI , GUENEAU FRANCK , RAVN ULLA , ELSON GREG
发明人： FISCHER NICOLAS , MAGISTRELLI GIOVANNI , GUENEAU FRANCK , RAVN ULLA , ELSON GREG
IPC分类号： C07K16/00 , C07K16/46
CPC分类号： C07K16/468 , C07K16/005 , C07K16/248 , C07K16/249 , C07K16/2866 , C07K2317/31 , C07K2317/32 , C07K2317/622 , C07K2317/92 , C12N15/1037 , C12N15/1075
摘要： The invention provides novel bispecific monoclonal antibodies carrying a different specificity for each binding site of the immunoglobulin molecule and methods for producing novel bispecific monoclonal antibodies carrying a different specificity for each binding site of the immunoglobulin molecule. The antibodies are composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other of a Lambda constant domain. The invention provides methods for the isolation of antibodies of different specificities but sharing a common heavy chain. The invention also provides methods for the controlled co-expression of two light chains and a single heavy chain leading to the assembly of monospecific and bispecific antibodies. The invention provides a mean of producing a fully human bispecific and bivalent antibody that is unaltered in sequence and does not involve the use of linkers or other non-human sequences, as well as antibody mixtures of two monospecific antibodies and one bispecific antibody. The invention also provides the means of efficiently purifying the bispecific antibody.
摘要翻译：本发明提供对免疫球蛋白分子的每个结合位点携带不同特异性的新型双特异性单克隆抗体以及用于产生对免疫球蛋白分子的每个结合位点具有不同特异性的新型双特异性单克隆抗体的方法。抗体由单个重链和两个不同的轻链组成，一个含有κ恒定结构域，另一个是λ恒定结构域。本发明提供了分离不同特异性但共享共同重链的抗体的方法。本发明还提供了两个轻链和单个重链的受控共表达的方法，导致单特异性和双特异性抗体的组装。本发明提供了产生完全人双特异性和二价抗体的平均值，其依次不变，不涉及使用接头或其它非人序列，以及两种单特异性抗体和一种双特异性抗体的抗体混合物。本发明还提供有效纯化双特异性抗体的方法。

3. 发明申请

WO2013136186A3 READILY ISOLATED BISPECIFIC ANTIBODIES WITH NATIVE IMMUNOGLOBULIN FORMAT 审中-公开
标题翻译：具有本身免疫球蛋白形式的自分离分离抗体
公开(公告)号：WO2013136186A3
公开(公告)日：2014-01-23
申请号：PCT/IB2013000902
申请日：2013-03-13
申请人： NOVIMMUNE SA , FISCHER NICOLAS , MAGISTRELLI GIOVANNI , ROUSSEAU FRANCOIS , MASTERNAK KRZYSZTOF , MALINGE PAULINE
发明人： FISCHER NICOLAS , MAGISTRELLI GIOVANNI , ROUSSEAU FRANCOIS , MASTERNAK KRZYSZTOF , MALINGE PAULINE
IPC分类号： C07K16/24 , C07K16/28
CPC分类号： C07K16/468 , C07K16/244 , C07K16/2809 , C07K2317/31
摘要： The invention relates to antigen-binding proteins or antibodies having heterodimers of heavy chains, i.e., two immunoglobulin heavy chains that differ by at least one or two amino acid(s) that allows for isolation of the antigen-binding protein based on a differential affinity of an immunoglobulin heavy chain and a modified/mutated immunoglobulin heavy chain toward an affinity reagent. The invention also relates antigen-binding proteins, including bispecific antibodies, having IgG CH1 regions with different affinities with respect to affinity reagent(s) that allows rapid isolation by differential binding of the IgG regions to the affinity reagent(s).
摘要翻译：本发明涉及具有重链异源二聚体的抗原结合蛋白或抗体，即两条免疫球蛋白重链之间有差异的至少一个或两个氨基酸，其允许基于差异亲和力分离抗原结合蛋白的免疫球蛋白重链和修饰/突变的免疫球蛋白重链朝向亲和试剂。本发明还涉及包含双特异性抗体的抗原结合蛋白，其具有相对于亲和试剂具有不同亲和力的IgG CH1区域，其允许通过IgG区域与亲和试剂的差异结合快速分离。

4. 发明申请

WO0131003B1 CLONING, EXPRESSION AND CHARACTERISATION OF A GENE EXPRESSED IN TUMOUR CELLS AND INVOLVED IN THE REGULATION OF THE IMMUNE RESPONSE 审中-公开
标题翻译：肿瘤细胞表达的基因的克隆，表达和表征及参与免疫应答的调控
公开(公告)号：WO0131003B1
公开(公告)日：2001-10-11
申请号：PCT/FR0003032
申请日：2000-10-30
申请人： PF MEDICAMENT , DELNESTE YVES , MAGISTRELLI GIOVANNI , JEANNIN PASCALE , BONNEFOY JEAN YVES
发明人： DELNESTE YVES , MAGISTRELLI GIOVANNI , JEANNIN PASCALE , BONNEFOY JEAN-YVES
IPC分类号： G01N33/50 , A61K31/7088 , A61K35/76 , A61K38/00 , A61K39/395 , A61K48/00 , A61P31/00 , A61P31/04 , A61P31/10 , A61P31/12 , A61P33/00 , A61P35/00 , A61P37/00 , A61P37/02 , C07K14/47 , C07K14/705 , C07K16/30 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/12 , C12P21/02 , C12Q1/68 , G01N33/15 , G01N33/53 , A01K67/027 , A61K38/17 , C12N15/11 , G01N33/68
CPC分类号： C07K14/705 , A01K2217/05 , A61K38/00
摘要： The invention concerns the identification and the characterisation of a novel gene expressed in tumour cells and involved in the regulation of the immune response, the cloning and the characterisation of its cDNA and the corresponding polypeptides. The invention also concerns vectors containing a nucleic acid coding for said gene, cells transformed by said vectors and diagnostic methods and selection methods of a chemical or biological compound capable of directly or indirectly interacting with a nucleic acid or a polypeptide of the invention. The invention further concerns compounds for treating immune pathologies, in particular autoimmune diseases and cancer.
摘要翻译：本发明涉及肿瘤细胞中表达的新基因的鉴定和表征，其涉及调节免疫应答，克隆和表达其cDNA和相应的多肽。本发明还涉及含有编码所述基因的核酸，由所述载体转化的细胞的载体以及能够与本发明的核酸或多肽直接或间接相互作用的化学或生物化合物的诊断方法和选择方法。本发明还涉及用于治疗免疫病状，特别是自身免疫性疾病和癌症的化合物。

5. 发明申请

WO2015033223A3 READILY ISOLATED BISPECIFIC BINDING MOLECULES WITH NATIVE FORMAT HAVING MUTATED CONSTANT REGIONS 审中-公开
标题翻译：准备隔离的双特异性结合分子与本地格式具有变异的恒定区域
公开(公告)号：WO2015033223A3
公开(公告)日：2015-10-29
申请号：PCT/IB2014002388
申请日：2014-09-03
申请人： NOVIMMUNE SA
发明人： FISCHER NICOLAS , MAGISTRELLI GIOVANNI , ROUSSEAU FRANÇOIS , MASTERNAK KRZYSZTOF , MALINGE PAULINE
IPC分类号： C07K16/24 , C07K1/00 , C07K16/28 , C07K16/46
CPC分类号： C07K16/468 , C07K16/244 , C07K16/2809 , C07K2317/21 , C07K2317/31 , C07K2317/50 , C07K2317/522 , C07K2317/526 , C07K2317/622 , C07K2317/94
摘要： The invention provides heterodimer bispecific antigen-binding molecules that include a first polypeptide thai does not include an IgG CH1 domain and a second polypeptide having an immunoglobulin constant region where there is at least one mutation in the IgG CH3 domain that abolishes the ability of the second polypeptide to bind CH3- specific affinity media such that the first and second polypeptides have different affinities with respect to CH1 and CH3 specific affinity reagents that allows rapid isolation by differential binding of the first and second polypeptides to these affinity reagents. The invention also provides bispeeific antibodies that have IgG CH1 and CH3 regions with different affinities with respect to affinity reagents that allows rapid isolation by differential binding of the IgG regions to these affinity reagents. The invention also concerns bispeeific antibodies which are heterodimers of heavy chains, i.e., two immunoglobulin heavy chains that differ by at least two amino acids that allowr for the isolation of the bispeeific antibody based on a differential affinity of one mutated immunoglobulin heavy chain and a second mutated immunoglobulin heavy chain toward two different affinity reagents.
摘要翻译：本发明提供了异二聚体双特异性抗原结合分子，其包括不包含IgG CH1结构域的第一多肽和具有免疫球蛋白恒定区的第二多肽，其中IgG CH3结构域中存在至少一个突变，其消除了第二多肽结合CH3特异性亲和介质，使得第一和第二多肽对于CH1和CH3特异性亲和试剂具有不同的亲和力，其允许通过第一和第二多肽与这些亲和试剂的差异结合来快速分离。本发明还提供具有对于亲和试剂具有不同亲和力的IgG CH1和CH3区的双特异性抗体，其允许通过IgG区与这些亲和试剂的差异结合来快速分离。本发明还涉及作为重链异二聚体的双特异性抗体，即两条免疫球蛋白重链相差至少两个氨基酸，允许基于一种突变的免疫球蛋白重链与第二种突变的免疫球蛋白重链的差异亲和力分离双特异性抗体突变的免疫球蛋白重链朝向两种不同的亲和试剂。

6. 发明专利

AU2017279804B2 Readily Isolated Bispecific Antibodies with Native Immunoglobulin Format 未知
公开(公告)号：AU2017279804B2
公开(公告)日：2020-03-12
申请号：AU2017279804
申请日：2017-12-22
申请人： NOVIMMUNE SA
发明人： FISCHER NICOLAS , MAGISTRELLI GIOVANNI , ROUSSEAU FRANCOIS , MASTERNAK KRZYSZTOF , MALINGE PAULINE
IPC分类号： C07K16/24 , C07K16/28
摘要： The invention relates to antigen-binding proteins or antibodies having heterodimers of heavy chains, i.e., two immunoglobulin heavy chains that differ by at least one or two amino acid(s) that allows for isolation of the antigen-binding protein based on a differential affinity of an immunoglobulin heavy chain and a modified/mutated immunoglobulin heavy chain toward an affinity reagent. The invention also relates antigen-binding proteins, including bispecific antibodies, having IgG CH Iregions with different affinities with respect to affinity reagent(s) that allows rapid isolation by differential binding of the IgG regions to the affinity reagent(s).

7. 发明专利

AU2018303254A1 Generating multispecific antibody mixtures and methods of uses thereof 未知
公开(公告)号：AU2018303254A1
公开(公告)日：2020-03-05
申请号：AU2018303254
申请日：2018-07-23
申请人： NOVIMMUNE SA
发明人： FISCHER NICOLAS , MAGISTRELLI GIOVANNI , MASTERNAK KRZYSZTOF
IPC分类号： C07K16/00 , A61P35/00 , C07K16/28 , C07K16/30
摘要： The invention relates to the generation of multispecific antibody mixtures include a subset of antibodies isolated from a mixture of two or more monospecific antibodies and one or more bispecific antibodies, wherein all antibodies in the subset have the same common heavy chain. The invention also relates to methods of isolating, purifying, or otherwise producing such a subset of antibodies by using at least one afÍinity chromatography step. The invention also relates to methods of using such a subset of antibodies in a variety of therapeutic indications.

8. 发明专利

MX338953B METODOS PARA LA GENERACION DE ANTICUERPOS MULTIESPECIFICOS Y MULTIVALENTES. 未知
公开(公告)号：MX338953B
公开(公告)日：2016-05-06
申请号：MX2013001900
申请日：2011-08-16
申请人： NOVIMMUNE SA
发明人： FISCHER NICOLAS , ELSON GREG , RAVN ULLA , GUENEAU FRANCK , MAGISTRELLI GIOVANNI
IPC分类号： C07K16/00 , C07K16/24 , C07K16/28 , C07K16/46 , C12N15/10
摘要： La presente invención se refiere a anticuerpos monoclonales biespecíficos novedosos que tienen una especificidad diferente para cada sitio de unión de la molécula de inmunoglobulina y métodos para producir anticuerpos monoclonales biespecíficos novedosos que tienen una especificidad diferente para cada sitio de unión de la molécula de inmunoglobulina; los anticuerpos se componen de una sola cadena pesada y dos diferentes cadenas ligeras, una que contiene un dominio constante Kappa y la otra un dominio constante Lambda; la invención proporciona métodos para el aislamiento de los anticuerpos de las especificidades diferentes pero compartiendo una cadena pesada común; la invención también proporciona métodos para la co-expresión controlada de dos cadenas ligeras y una cadena pesada sencilla lo que lleva al montaje de anticuerpos monoespecíficos y biespecíficos. la invención proporciona un medio para producir un anticuerpo bivalente y biespecífico completamente humano que es inalterado en la secuencia y no implica el uso de enlazadores u otras secuencias no humanos, así como mezclas de anticuerpos de dos anticuerpos monoespecíficos y un anticuerpo biespecífico; la invención también proporciona los medios de purificación eficientemente del anticuerpo biespecífico.

9. 发明专利

PT2606064E PROCESSOS PARA A GERAÇÃO DE ANTICORPOS POLIESPECÍFICOS E POLIVALENTES 未知
公开(公告)号：PT2606064E
公开(公告)日：2015-06-08
申请号：PT11788226
申请日：2011-08-16
申请人： NOVIMMUNE SA
发明人： ELSON GREG , FISCHER NICOLAS , MAGISTRELLI GIOVANNI , GUENEAU FRANCK , RAVN ULLA
IPC分类号： C07K16/00 , C07K16/24 , C07K16/28 , C07K16/46 , C12N15/10

10. 发明专利

ES2752054T3 Método para optimizar el ensamble y producción de complejos de proteínas heteromultiméricas 未知
公开(公告)号：ES2752054T3
公开(公告)日：2020-04-02
申请号：ES16715820
申请日：2016-03-31
申请人： NOVIMMUNE SA
发明人： MAGISTRELLI GIOVANNI , MALINGE PAULINE , POITEVIN YVES , FISCHER NICOLAS
IPC分类号： C12N15/67 , C07K16/46 , C12P21/02
摘要： Un método para aumentar el rendimiento de producción de un complejo de proteínas que comprende: (a) identificar uno o más polipéptidos en el complejo que se expresan más abundantemente en una célula en comparación con los otros polipéptidos en el complejo; (b) modificar al menos una molécula de ácido nucleico que codifica los polipéptidos más abundantemente expresados en el complejo, en el que las moléculas de ácido nucleico se modifican al alterar la composición de codón de las moléculas de ácido nucleico que resulta en una reducción de la expresión de los polipéptidos que codifica; (c) introducir las moléculas de ácido nucleico en una célula; y (d) cultivar la célula bajo condiciones que permiten la expresión de todos los polipéptidos del complejo de proteínas; en el que dicha reducción de la expresión se logra mediante el reemplazo de ciertos codones con codones que se utilizan con menos frecuencia en la célula, al codificar un cambio en la estructura secundaria del ARNm o codificar un cambio en la tasa de traducción, y en el que el complejo de proteínas es un anticuerpo biespecífico.

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