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1. 发明申请

WO1996023221A1 POLYVINYLALCOHOL COATED CAPILLARY ELECTROPHORESIS COLUMNS 审中-公开
标题翻译：聚乙烯醇包衣毛细管电泳柱
公开(公告)号：WO1996023221A1
公开(公告)日：1996-08-01
申请号：PCT/US1996001347
申请日：1996-01-23
申请人： BECKMAN INSTRUMENTS, INC. , NORTHEASTERN UNIVERSITY , NORTHEASTERN UNIVERSITY , KARGER, Barry, L. , GOETZINGER, Wolfgang , MUTH, Jochen , SHIEH, Chia-Hui
发明人： BECKMAN INSTRUMENTS, INC. , NORTHEASTERN UNIVERSITY , NORTHEASTERN UNIVERSITY
IPC分类号： G01N27/477
CPC分类号： G01N27/44704 , G01N27/44752
摘要： Coated capillary electrophoresis columns and methods for their use in electrophoretic separations are disclosed. The coated capillary columns include a length of tubing having an interior surface having an interconnected polymeric coating. The interconnected polymeric coating includes a hydrophobic hydrocarbon polymeric functionality covalently bound to the interior surface and polyvinylalcohol interconnected with the hydrophobic polymeric functionality. Exemplary columns are prepared by causing a Si-OH reactive compound, having a hydrophobic polymeric functionality, to react with Si-OH functionalities on the interior surface of capillary columns. Then causing vinyl acetate to polymerize in contact with the hydrophobic polymeric functionality and hydrolyzing the resulting polyvinylacetate forms a coating of interconnecting hydrophilic and hydrophobic polymers. Alternative exemplary coatings include a modified polyvinylalcohol such as a polyvinylether.
摘要翻译：公开了涂布毛细管电泳柱及其用于电泳分离的方法。涂覆的毛细管柱包括具有内表面的一段长度的管，其具有互连的聚合物涂层。相互连接的聚合物涂层包括与内表面共价结合的疏水性烃聚合物官能团和与疏水性聚合物官能团互连的聚乙烯醇。通过使具有疏水聚合官能度的Si-OH反应性化合物与毛细管柱内表面上的Si-OH官能团反应来制备示例性柱。然后使乙酸乙烯酯与疏水聚合官能团接触聚合并水解所得聚乙酸乙烯酯形成互连的亲水和疏水聚合物的涂层。替代的示例性涂层包括改性的聚乙烯醇，例如聚乙烯醚。

2. 发明申请

WO1998010122A1 MICROFABRICATED HYBRID CAPILLARY ARRAY AND MULTICHANNEL DETECTION ASSEMBLY 审中-公开
标题翻译：微型混合毛细管阵列和多通道检测装置
公开(公告)号：WO1998010122A1
公开(公告)日：1998-03-12
申请号：PCT/US1997015461
申请日：1997-09-03
申请人： NORTHEASTERN UNIVERSITY
发明人： NORTHEASTERN UNIVERSITY , KARGER, Barry, L. , LIU, Shaorong , FORET, Frantisek , XUE, Quifeng
IPC分类号： C25B09/00
CPC分类号： G01N27/44717 , G01N27/44721 , G01N27/44743 , G01N27/44791
摘要： A hybrid microfabricated substrate capillary array assembly, which provides an interface between the integrated channels (12) on a microfabricated substrate (10), e.g., microchip, and flexible capillaries (18), is disclosed. The hybrid device permits, e.g., convenient injection of samples from a capillary array (18) into channels (12) in a microchip (10) and also enables, e.g., convenient detection on the device by LIF (laser induced fluorescence). With the use of such an assembly, large numbers of samples may be processed simultaneously, leading to high-speed, high-throughput analyses.
摘要翻译：公开了一种混合微制造的基底毛细管阵列组件，其提供微加工基板（10）上的集成通道（12），例如微芯片和柔性毛细管（18）之间的界面。混合装置允许例如将样品从毛细管阵列（18）方便地注入到微芯片（10）中的通道（12）中，并且还能够例如通过LIF（激光诱导荧光）在装置上方便地检测。通过使用这种组件，可以同时处理大量样品，从而实现高速，高通量分析。

3. 发明申请

WO1997026072A1 SUBATMOSPHERIC, VARIABLE PRESSURE SAMPLE DELIVERY CHAMBER FOR ELECTROSPRAY IONIZATION/MASS SPECTROMETRY AND OTHER APPLICATIONS 审中-公开
标题翻译：用于电喷雾离子/质谱分析和其他应用的可变压力样品输送室
公开(公告)号：WO1997026072A1
公开(公告)日：1997-07-24
申请号：PCT/US1997000733
申请日：1997-01-17
申请人： NORTHEASTERN UNIVERSITY
发明人： NORTHEASTERN UNIVERSITY , KARGER, Barry, L. , FORET, Frantisek , KIRBY, Daniel, P.
IPC分类号： B01D59/44
CPC分类号： H01J49/045 , G01N27/44717 , G01N30/7266 , G01N2030/285 , H01J49/165
摘要： A subatmospheric, variable pressure sample delivery chamber (2), useful as an electrospray ionization device for introducing a sample into a mass spectrometer (9) or as a sample device at subatmospheric pressures, is disclosed. The sample delivery chamber (2) is configured to maintain an operating pressure between 1 and 750 Torr, preferably between 50 and 700 Torr. The chamber has an inlet port (4) for introduction of a gas and an exit port (6) capable of being coupled either to a sampling orifice (8) for an analytical device, usually a mass spectrometer (9), or directly to a pump for removal of gas. A sample delivery device (22) is capable of receiving a sample and the exit port (6) of the chamber, if the sample is to be delivered to an external analytical device, or aligned with the collection device, for direct collection of the sample.
摘要翻译：公开了一种低于大气压的可变压力样品输送室（2），其用作将样品引入质谱仪（9）或作为低于大气压的样品装置的电喷雾离子化装置。样品输送室（2）被构造成将操作压力保持在1和750托之间，优选在50和700托之间。腔室具有用于引入气体的入口（4）和能够耦合到用于分析装置（通常为质谱仪（9））的采样孔（8）的出口（6），或直接连接到泵用于除气。样品输送装置（22）能够接收样品和室的出口（6），如果要将样品输送到外部分析装置或与收集装置对准，以便直接收集样品。

4. 发明申请

WO1990012574A1 DOPAMINE AGONIST COMPOUNDS 审中-公开
标题翻译：多巴胺激动剂化合物
公开(公告)号：WO1990012574A1
公开(公告)日：1990-11-01
申请号：PCT/US1989001747
申请日：1989-04-25
申请人： NORTHEASTERN UNIVERSITY
发明人： NORTHEASTERN UNIVERSITY , NEUMEYER, John, L. , RAMSBY, Sten
IPC分类号： A61K31/47
CPC分类号： C07D491/06 , C07D221/18
摘要： Orally effective dopamine agonist compounds are described including in particular novel aporphine compounds. The compounds have a dioxy group on an aromatic nucleus which is cleaved in vivo to release compounds with two adjacent hydroxy groups on the aromatic nucleus. Also described are novel dopamine agonist aporphine compounds wherein the phenolic hydroxyl group is replaced by amino, bromo or fluoro groups.
摘要翻译：描述了口腔有效的多巴胺激动剂化合物，特别是包括新的阿托啡化合物。该化合物在芳族核上具有二氧基团，其在体内被切割以释放在芳香核上具有两个相邻羟基的化合物。还描述了其中酚羟基被氨基，溴或氟基团取代的新型多巴胺激动剂阿朴肽化合物。

5. 发明申请

WO1997004297A1 MICROSCALE FLUID HANDLING SYSTEM 审中-公开
标题翻译：微型流体处理系统
公开(公告)号：WO1997004297A1
公开(公告)日：1997-02-06
申请号：PCT/US1996011985
申请日：1996-07-19
申请人： NORTHEASTERN UNIVERSITY
发明人： NORTHEASTERN UNIVERSITY , KARGER, Barry, L. , FORET, Frantisek , ZAVRACKY, Paul, M. , MCGRUER, E., Nicol , XUE, Qifeng , DUNAYEVSKIY, Yuriy, M.
IPC分类号： G01N01/14
CPC分类号： H01J49/165 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00824 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/00853 , B01J2219/00873 , B01J2219/00889 , B01J2219/00891 , B01J2219/0093 , B01J2219/00932 , B01L3/0268 , B01L3/502715 , B01L2300/0803 , B01L2300/0816 , B01L2400/027 , B01L2400/0421 , B33Y80/00 , G01N30/466 , G01N30/6095 , G01N30/7266 , G01N2030/027 , G01N2030/285 , G01N2030/628 , G01N2035/1044 , H01J49/0445 , Y10T436/117497 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , Y10T436/24 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , G01N30/34
摘要： A microscale fluid handling system (10) that permits the efficient transfer of nanoliter to picoliter quantities of a fluid sample from the spatially concentrated environment of a microfabricated chip to "off-chip" analytical or collection devices (23) for further off-chip sample manipulation and analysis is disclosed. The fluid handling system (10) is fabricated in the form of one or more channels (12), in any suitable format, provided in a microchip body or substrate of silica, polymer or other suitable non-conductive material, or of stainless steel, noble metal, silicon or other suitable conductive or semi-conductive material. The microchip fluid handling system (10) includes one or more exit ports (16) integral with the end of one or more of the channels (12) for consecutive or simultaneous off-chip analysis or collection of the sample. The exit port or ports (16) may be configured, for example, as an electrospray interface for transfer of a fluid sample to a mass spectrometer (23).
摘要翻译：微量流体处理系统（10），其允许将纳升酯有效转移至微胶囊数量的流体样品，从微细堆芯片的空间集中环境到“片外”分析或收集装置（23），用于进一步的片外样品披露了操作和分析。液体处理系统（10）以一种或多种通道（12）的形式制成，其格局为任何合适的格式，设置在硅片，聚合物或其它合适的非导电材料或不锈钢的微芯片体或基底中，贵金属，硅或其他合适的导电或半导体材料。微芯片流体处理系统（10）包括与一个或多个通道（12）的端部成一体的一个或多个出口（16），用于连续或同时片外分析或收集样品。出口或端口（16）可以被配置为例如用于将流体样品转移到质谱仪（23）的电喷雾界面。

6. 发明申请

WO1996021002A1 METHODS FOR PRODUCING IMPROVED STRAINS OF SEAWEED, AND RESULTANT SEAWEEDS AND PHYCOCOLLOIDS 审中-公开
标题翻译：用于生产改良的海藻和成虫海藻和藻类的方法
公开(公告)号：WO1996021002A1
公开(公告)日：1996-07-11
申请号：PCT/US1995000054
申请日：1995-01-04
申请人： NORTHEASTERN UNIVERSITY
发明人： NORTHEASTERN UNIVERSITY , CHENEY, Donald, P. , DUKE, Clifford
IPC分类号： C12N05/04
CPC分类号： C12N5/14 , A01H13/00 , C12N15/8201
摘要： Methods for producing hybrid and transgenic strains of marine algae, and seaweeds and phycocolloids produced by the product seaweeds. Hybrid marine algae are produced by preparing protoplasts from spores of parental algal plants, fusing the spore-protoplast to form heterokaryon fusion products, isolating selected fusion products, and culturing these to produce multicellular material. Similarly, transgenic marine algae are produced by preparing protoplasts from spores of a marine algal plant, introducing foreign DNA into the resulting spore-protoplasts to form transformed spore-protoplasts, selecting certain transformed spore-protoplasts, and culturing these to produce transgenic multicellular material.
摘要翻译：用于产生海藻的杂交和转基因品种的方法，以及由产品海藻生产的海藻和藻类。通过从亲本藻类植物的孢子制备原生质体，将孢子原生质体融合以形成异核融合产物，分离选择的融合产物，并培养它们以产生多细胞材料来产生杂种海藻。类似地，通过从海藻藻植物的孢子制备原生质体，将外源DNA引入所得的孢子原生质体中以形成转化的孢子原生质体，选择某些转化的孢子原生质体，并培养它们以产生转基因多细胞材料，从而产生转基因海藻。

7. 发明申请

WO1997039481A1 AN INTEGRATED COMPLEX-TRANSITION METAL OXIDE DEVICE AND A METHOD OF FABRICATING SUCH A DEVICE 审中-公开
标题翻译：一体化复合过渡金属氧化物装置及其制造方法
公开(公告)号：WO1997039481A1
公开(公告)日：1997-10-23
申请号：PCT/US1997005961
申请日：1997-04-10
申请人： NORTHEASTERN UNIVERSITY
发明人： NORTHEASTERN UNIVERSITY , OLIVER, Steven, A. , ZAVRACKY, Paul , McGRUER, Nicol, E. , VITTORIA, Carmine
IPC分类号： H01L21/44
CPC分类号： C03C17/3642 , C03C17/36 , C03C17/3652 , C04B35/62222 , C04B41/009 , C04B41/4511 , C04B41/81 , C04B2111/00844 , C04B41/5027 , C04B35/00
摘要： A method for the fabrication of complex-transition metal oxide (CTMO)/semiconductor or dielectric substrate integrated devices includes the separation of the CTMO film growth process from the CTMO-film/semiconductor or dielectric substrate integration process. The CTMO-film (10) is transferred from the native substrate (12) to the final substrate (14) for fabrication into devices. The CTMO-film (10) is grown onto a native substrate (12) under growth conditions chosen to provide a CTMO-film having desirable properties and thickness. No restrictions are placed upon the native substrate used, the growth method used, or on the growth conditions required. The CTMO-film (10) is then joined to the semiconductor or dielectric substrate (14) and the native substrate (12) is removed, providing the basis for an integrated electronics, photonics, or MEMS device. Techniques fully compatible with semiconductor processing can be used to fabricate monolithically integrated CTMO/semiconductor devices exemplified in first embodiment.
摘要翻译：复合过渡金属氧化物（CTMO）/半导体或电介质基板集成器件的制造方法包括将CTMO膜生长工艺与CTMO膜/半导体或电介质衬底集成工艺分开。 CTMO膜（10）从天然衬底（12）转移到最终衬底（14）以制造成器件。 CTMO膜（10）在生长条件下生长到天然基质（12）上，以提供具有所需性质和厚度的CTMO膜。对所用的天然底物，所用的生长方法或所需的生长条件没有限制。然后将CTMO膜（10）接合到半导体或电介质基板（14），并去除天然基板（12），为集成电子，光子学或MEMS器件提供基础。与半导体处理完全兼容的技术可以用于制造在第一实施例中示例的单片集成CTMO /半导体器件。

8. 发明申请

WO1995011901A1 SUBSTITUTED 2-CARBOXYALKYL-3-(FLUOROPHENYL)-8-(3-HALOPROPEN-2-YL) NORTROPANES AND THEIR USE AS IMAGING AGENTS FOR NEURODEGENERATIVE DISORDERS 审中-公开
标题翻译：取代的2-羧甲基-3-（氟代戊基）-8-（3-庚二炔-2-基）诺酮和它们作为神经损伤药物的成像剂
公开(公告)号：WO1995011901A1
公开(公告)日：1995-05-04
申请号：PCT/US1994012225
申请日：1994-10-24
申请人： THE GENERAL HOSPITAL CORPORATION , THE PRESIDENT AND FELLOWS OF HARVARD COLLEGE , ORGANIX, INC. , NORTHEASTERN UNIVERSITY
发明人： THE GENERAL HOSPITAL CORPORATION , THE PRESIDENT AND FELLOWS OF HARVARD COLLEGE , ORGANIX, INC. , NORTHEASTERN UNIVERSITY , ELMALEH, David, R. , MADRAS, Bertha, K. , MELTZER, Peter , HANSON, Robert, N.
IPC分类号： C07D451/02
CPC分类号： A61K51/0448 , A61K2123/00 , C07D451/02
摘要： Disclosed are certain cocaine analogs useful for imaging of cocaine receptors and dopamine receptors. Also disclosed are analogs useful for imaging diagnostics for Parkinson's disease.
摘要翻译：公开了某些可卡因类似物可用于成像可卡因受体和多巴胺受体。还公开了可用于帕金森病成像诊断的类似物。

9. 发明申请

WO1994028162A1 IDENTIFICATION OF THERAPEUTIC AGENTS EFFECTIVE AGAINST INFECTION BY MEMBERS OF THE PARASITE FAMILY TRYPANOSOMATIDAE 审中-公开
标题翻译：鉴定有效防治寄生虫寄生虫成虫的治疗药物
公开(公告)号：WO1994028162A1
公开(公告)日：1994-12-08
申请号：PCT/US1994005624
申请日：1994-05-19
申请人： NORTHEASTERN UNIVERSITY
发明人： NORTHEASTERN UNIVERSITY , STRAUSS, Phyllis, R.
IPC分类号： C12Q01/00
CPC分类号： C12Q1/18 , C12Q1/34
摘要： DNA polymerase beta isolated from a member of the parasite family Trypanosomatidae, the species Trypanosoma brucei, has been determined to be the primary drug target for the trypanocidal agent berenil (diminazene aceturate), being four orders of magnitude more sensitive to the drug than is either human DNA polymerase beta or alpha . DNA polymerases of the alpha family isolated from T. brucei, also effective targets of berenil, are two orders of magnitude more sensitive than either of the human enzymes. The discovery of the differential sensitivity of the DNA polymerases from T. brucei to a trypanocidal agent has permitted the development of methods for systematically identifying and using therapeutic agents effective in treating a patient suffering from an infection by a member of this family of parasites. The methods include testing the sensitivity of DNA polymerase alpha and DNA polymerase beta from a member of the family Trypanosomatidae to a candidate trypanocidal agent, comparing the determined sensitivity of the DNA polymerases to the candidate agent, and testing the DNA polymerase having greater sensitivity against decreasing levels of the candidate agent. In this way a minimal effective dosage of the agent can be determined.
摘要翻译：从寄生虫家族Trypanosomatidae（Brypanosoma trypanosoma brucei）的成员中分离的DNA聚合酶β已被确定为杀虫剂be il（二亚胺乙酸酯）的主要药物靶标，比药物更敏感4个数量级人类DNA聚合酶β或α。从布鲁氏菌中分离出来的α家族的DNA聚合酶也是贝尼尔的有效靶标，比人类酶的敏感性高两个数量级。发现从布鲁斯布氏锥虫病到杀虫剂的DNA聚合酶的差异敏感性允许开发用于系统地鉴定和使用治疗剂的方法，所述治疗剂有效地治疗患有该寄生虫家族的感染的患者。这些方法包括测试DNA聚合酶α和DNA聚合酶β从锥虫科科的成员对候选的杀虫剂的敏感性，比较确定的DNA聚合酶对候选试剂的敏感性，并测试具有较高灵敏度降低的DNA聚合酶候选人的级别。以这种方式，可以确定药剂的最小有效剂量。

10. 发明申请

WO1994017409A1 QUANTIFYING TRACE ANALYTES BY AFFINITY CAPILLARY ELECTROPHORESIS 审中-公开
标题翻译：定量毛细管电泳分析痕量分析
公开(公告)号：WO1994017409A1
公开(公告)日：1994-08-04
申请号：PCT/US1994000426
申请日：1994-01-12
申请人： NORTHEASTERN UNIVERSITY
发明人： NORTHEASTERN UNIVERSITY , KARGER, Barry, L. , SHIMURA, Kiyohito
IPC分类号： G01N33/561
CPC分类号： G01N27/44795 , G01N27/44726 , G01N33/533 , G01N33/561 , G01N33/6857
摘要： A method for quantitative detection of trace amounts of an analyte in a sample is disclosed. The method in the preferred embodiment includes providing an Fab' fragment of an immunoglobulin labelled at a reactive sulfhydryl group with a fluorescent dye; combining the labelled Fab' fragment with a sample that may contain the analyte; concentrating the elements of the resulting mixture in an electric field; separating the labelled analyte/agent complex formed from any unreacted labelled agent using capillary electrophoretic methods; and detecting the fluorescent signal of the labelled analyte/agent complex. The invention also is directed to a method of producing a labelled Fab' fragment that includes cleaving an immunoglobulin molecule to obtain one F(ab')2 fragment; reducing the disulfide-bonds of the F(ab')2 fragment to obtain two Fab' fragments each having at least one free, reactive sulfhydryl group; and mixing an Fab' fragment having at least one free sulfhydryl group with a fluorescent dye reactive with the free sulfhydryl to form a labelled Fab' fragment. Preferably, prior to the final mixing step, intrastrand disulfide bonds are formed by oxidation within each Fab' fragment, thereby producing individual Fab' fragments each having a single reactive sulfhydryl group. The method of quantitative detection also more broadly includes using any biospecific agent to form a complex with the target analyte.
摘要翻译：公开了一种用于定量检测样品中微量分析物的方法。优选实施方案中的方法包括提供在反应性巯基上标记的免疫球蛋白的Fab'片段与荧光染料; 将标记的Fab'片段与可以含有分析物的样品结合; 将所得混合物的元素集中在电场中; 使用毛细管电泳方法分离由任何未反应的标记试剂形成的标记分析物/试剂络合物; 并检测标记的分析物/试剂复合物的荧光信号。本发明还涉及产生标记的Fab'片段的方法，其包括切割免疫球蛋白分子以获得一个F（ab'）2片段; 减少F（ab'）2片段的二硫键，得到两个具有至少一个游离的反应性巯基的Fab'片段; 并将具有至少一个游离巯基的Fab'片段与与游离巯基反应的荧光染料混合以形成标记的Fab'片段。优选地，在最终混合步骤之前，通过在每个Fab'片段内的氧化形成原子间二硫键，由此产生各自具有单个反应性巯基的单个Fab'片段。定量检测的方法也更广泛地包括使用任何生物特异性试剂与靶分析物形成复合物。

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