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1. 发明授权

US11136609B2 Separating agent for human serum-derived IgG polyclonal antibodies, and method for separating human serum-derived IgG polyclonal antibodies using same 有权
公开(公告)号：US11136609B2
公开(公告)日：2021-10-05
申请号：US15774398
申请日：2016-11-07
申请人： NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY , DAICEL CORPORATION
发明人： Yoichi Kumada , Yuya Hasegawa , Seiichi Uchimura
IPC分类号： C07K1/22 , C07K16/42 , C12P21/00 , C12Q1/70 , C12N15/09 , C07K16/06 , C12N15/62
摘要： An object of the present invention is to provide a separation agent for separating a human serum-derived IgG polyclonal antibody. This object is achieved by a separation agent for separating a human serum-derived IgG polyclonal antibody, the separation agent including: a carrier; and a single-chain antibody which has a dissociation constant for a human serum-derived IgG polyclonal antibody of not more than 3.0×10−8 M and which binds to the surface of the carrier via a chemical bond.

2. 发明授权

US12103948B2 Separating agent 有权
公开(公告)号：US12103948B2
公开(公告)日：2024-10-01
申请号：US17256599
申请日：2019-07-01
申请人： NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY , DAICEL CORPORATION
发明人： Yoichi Kumada , Hiromichi Okura , Seiichi Uchimura
IPC分类号： C07K1/22
CPC分类号： C07K1/22
摘要： Provided is a separating agent with an improved dynamic binding capacity (DBC) to a target substance. The separating agent includes a carrier and a protein, wherein the protein is a given protein, and a surface of the carrier and added lysine residues in the protein are bound by chemical bonds.

3. 发明申请

US20150376297A1 METHOD FOR REFOLDING ANTIBODY, PROCESS FOR PRODUCING REFOLDED ANTIBODY, REFOLDED ANTIBODY, AND USES THEREOF 有权
标题翻译：抗体抗体检测方法，生产抗体抗体的方法，抗体反应及其用途
公开(公告)号：US20150376297A1
公开(公告)日：2015-12-31
申请号：US14767373
申请日：2014-02-03
申请人： NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY
发明人： Yoichi Kumada , Yasuyuki Ishikawa , Yusuke Fujiwara , Michimasa Kishimoto
IPC分类号： C07K16/42 , C07K16/40 , C08F122/10 , C07K16/26 , C07K17/08 , C07K16/30 , C07K16/18
CPC分类号： C07K16/4241 , C07K16/065 , C07K16/18 , C07K16/26 , C07K16/3007 , C07K16/40 , C07K17/08 , C07K2317/14 , C07K2317/40 , C07K2317/622 , C07K2319/35 , C08F122/10
摘要： A method for refolding an antibody, a process for producing a refolded antibody, a refolded antibody, and uses thereof are provided. A method for refolding an antibody in a liquid phase comprises the steps of denaturing an inactive antibody binding directly or through a linker to a peptide, the peptide having an isoelectric point lower than the isoelectric point of the inactive antibody, and dispersing in a liquid phase the peptide-binding inactive antibody denatured in the step above. Also provided is a process for producing a refolded antibody.
摘要翻译：提供了重折叠抗体的方法，重折叠抗体的制备方法，重折叠抗体及其用途。用于在液相中重新折叠抗体的方法包括使直接结合或通过接头结合到肽的无活性抗体变性的步骤，所述肽具有低于无活性抗体的等电点的等电点，并分散在液相中在上述步骤中变性的肽结合的无活性抗体。还提供了生产重折叠抗体的方法。

4. 发明申请

US20150038675A1 PEPTIDE HAVING AFFINITY FOR SILICON NITRIDE (SI3N4), AND USE THEREOF 有权
标题翻译：具有氮化硅（SI3N4）的亲水性肽及其用途
公开(公告)号：US20150038675A1
公开(公告)日：2015-02-05
申请号：US14378433
申请日：2013-02-12
申请人： National University Corporation Kyoto Institute of Technology
发明人： Yoichi Kumada , Michimasa Kishimoto , Takeru Otsuka
IPC分类号： C07K17/14 , C07K7/08 , C07K7/06 , C07K14/245
CPC分类号： C07K17/14 , C07K7/06 , C07K7/08 , C07K14/245 , C07K2319/00 , C07K2319/23 , C12N9/0006 , C12N11/14
摘要： The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride. The invention involves a peptide having an affinity for silicon nitride, the peptide comprising (1-1) a peptide having the amino acid sequence of one of SEQ ID NOS: 1, 2 and 23 to 27, (1-2) a peptide that has an affinity for silicon nitride and comprises an amino acid sequence obtained by deleting, adding, and/or substituting one or more amino acids in one of the amino acid sequences indicated in (1-1), or a fragment of one of the peptides.
摘要翻译：本发明的目的是提供：对氮化硅具有亲和力的肽; 编码该肽的多核苷酸; 用于表达对氮化硅具有亲和性的肽的表达载体; 用于表达包含对氮化硅和靶蛋白具有亲和力的肽的肽融合蛋白的表达载体; 通过将表达载体导入宿主细胞获得的转化体; 从转化体获得的肽融合蛋白; 已经结合了对氮化硅具有亲和性的肽的氮化硅衬底; 将靶蛋白固定在氮化硅衬底上的方法; 用于将靶蛋白固定到氮化硅衬底的组合物，所述组合物包含对氮化硅具有亲和性的肽; 以及用于将靶蛋白固定到氮化硅衬底的接头，所述接头包含对氮化硅具有亲和性的肽。本发明涉及对氮化硅具有亲和力的肽，所述肽包含（1-1）具有SEQ ID NO：1,2和23至27之一的氨基酸序列的肽，（1-2）肽，对氮化硅具有亲和性，并且包含通过在（1-1）所示的氨基酸序列之一中缺失，添加和/或取代一个或多个氨基酸而获得的氨基酸序列，或其中一个肽的一个片段。

5. 发明申请

US20180031537A1 METHOD FOR SUPPRESSING PROTEIN ADSORPTION 审中-公开
公开(公告)号：US20180031537A1
公开(公告)日：2018-02-01
申请号：US15550445
申请日：2016-02-18
申请人： NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY , NOF CORPORATION
发明人： Yoichi Kumada , Yasutaka KAWAGUCHI , Aranna NEMOTO , Fumio NAKASHIMA , Nobuyuki SAKAMOTO
IPC分类号： G01N33/48 , C08F220/56 , C07F9/44 , C08F222/38
摘要： Provided is a novel method of suppressing non-specific protein adsorption. The above-mentioned object can be achieved by treating a solid phase with a solid phase-treating liquid containing a phosphorylcholine group-containing polymer as a main component, and treating a specimen with a specimen-treating liquid containing, as a main component, a polymer having a phosphorylcholine group, a hydroxy group, and a hydrophobic group, followed by addition of the treated specimen to the treated solid phase.

6. 发明授权

US10520486B2 Method for suppressing protein adsorption 有权
公开(公告)号：US10520486B2
公开(公告)日：2019-12-31
申请号：US15550445
申请日：2016-02-18
申请人： NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY , NOF CORPORATION
发明人： Yoichi Kumada , Yasutaka Kawaguchi , Aranna Nemoto , Fumio Nakashima , Nobuyuki Sakamoto
IPC分类号： G01N33/00 , G01N33/48 , C07F9/44 , C08F220/56 , C08F222/38
摘要： Provided is a novel method of suppressing non-specific protein adsorption. The above-mentioned object can be achieved by treating a solid phase with a solid phase-treating liquid containing a phosphorylcholine group-containing polymer as a main component, and treating a specimen with a specimen-treating liquid containing, as a main component, a polymer having a phosphorylcholine group, a hydroxy group, and a hydrophobic group, followed by addition of the treated specimen to the treated solid phase.

7. 发明授权

US09598501B2 Antibody-immobilized carrier, method of producing antibody-immobilized carrier, and use of said antibody-immobilized carrier 有权
公开(公告)号：US09598501B2
公开(公告)日：2017-03-21
申请号：US14502013
申请日：2014-09-30
申请人： NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY , DENKA SEIKEN CO., LTD.
发明人： Yoichi Kumada , Michimasa Kishimoto , Kyoko Hamasaki , Aya Nakagawa
IPC分类号： C07K17/00 , C07K16/00 , C07K16/18 , C07K16/28 , C07K16/40 , C07K16/24 , C07K16/30
CPC分类号： C07K17/00 , C07K16/00 , C07K16/18 , C07K16/249 , C07K16/2887 , C07K16/303 , C07K16/40 , C07K2317/21 , C07K2317/24 , C07K2317/51 , C07K2317/515 , C07K2317/55
摘要： The present invention provides an antibody-immobilized carrier that can be used in antibody screening, a method of producing the antibody-immobilized carrier, and use of the antibody-immobilized carrier. Efficient antibody screening can be carried out particularly by an antibody-immobilized carrier including two or more antibody immobilized regions onto each of which a heavy-chain low-molecular-weight antibody and a light-chain low-molecular-weight antibody are separately immobilized, the two or more antibody immobilized regions each being included in an independent manner, the heavy-chain low-molecular-weight antibody including a heavy-chain variable region, the light-chain low-molecular-weight antibody including a light-chain variable region, the heavy-chain low-molecular-weight antibody and the light-chain low-molecular-weight antibody each being derived from an antibody recognizing a different antigen.

8. 发明授权

US11078254B2 Method for immobilizing lectin 有权
公开(公告)号：US11078254B2
公开(公告)日：2021-08-03
申请号：US16604565
申请日：2018-04-11
申请人： National Institute of Advanced Industrial Science and Technology , Nissan Chemical Corporation , National University Corporation Kyoto Institute of Technology
发明人： Hiroaki Tateno , Junko Katayama , Kazutaka Matoba , Yoichi Kumada
IPC分类号： C07K7/06 , C07K14/195 , C07K17/08 , C07K17/14 , C07K19/00 , G01N33/545 , G01N33/551 , G01N33/566 , C07K14/705 , C07K17/00 , C12N15/62 , G01N33/543
摘要： Provided is a highly sensitive and less expensive lectin-immobilized base material (for example, a lectin plate), such as lectin-immobilized base material having stable qualities and being able to be sufficiently washed after a target sugar chain-containing antigen binds thereto. Further provided is a method for immobilizing lectin to a base material therefor. Particularly provided are: a method whereby a lectin-peptide fusion, in which a peptide capable of adsorbing to a base material surface such as a polystyrene (PS) tag is fused with the N-terminal side or C-terminal side of lectin capable of recognizing a target sugar chain, is immobilized on the peptide side to a base material; and a lectin-immobilized base material produced by this method. By using the lectin-immobilized base material, a target sugar chain-containing antigen can be highly sensitively and evenly measured and, moreover, target sugar chain-containing cells, etc. can be separated (concentrated and harvested).

9. 发明授权

US09850316B2 Method for refolding antibody, process for producing refolded antibody, refolded antibody, and uses thereof 有权
公开(公告)号：US09850316B2
公开(公告)日：2017-12-26
申请号：US14767373
申请日：2014-02-03
申请人： NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY
发明人： Yoichi Kumada , Yasuyuki Ishikawa , Yusuke Fujiwara , Michimasa Kishimoto
IPC分类号： C07K16/18 , C07K16/26 , C07K17/14 , C07K17/08 , C07K16/42 , C07K16/06 , C07K16/30 , C07K16/40 , C08F122/10
CPC分类号： C07K16/4241 , C07K16/065 , C07K16/18 , C07K16/26 , C07K16/3007 , C07K16/40 , C07K17/08 , C07K2317/14 , C07K2317/40 , C07K2317/622 , C07K2319/35 , C08F122/10
摘要： A method for refolding an antibody, a process for producing a refolded antibody, a refolded antibody, and uses thereof are provided. A method for refolding an antibody in a liquid phase comprises the steps of denaturing an inactive antibody binding directly or through a linker to a peptide, the peptide having an isoelectric point lower than the isoelectric point of the inactive antibody, and dispersing in a liquid phase the peptide-binding inactive antibody denatured in the step above. Also provided is a process for producing a refolded antibody.

10. 发明授权

US09701760B2 Peptide having affinity for silicon nitride (Si3N4), and use therefor 有权
公开(公告)号：US09701760B2
公开(公告)日：2017-07-11
申请号：US14378433
申请日：2013-02-12
申请人： National University Corporation Kyoto Institute of Technology
发明人： Yoichi Kumada , Michimasa Kishimoto , Takeru Otsuka
IPC分类号： C07K17/14 , C07K14/245 , C07K7/06 , C07K7/08 , C12N9/04 , C12N11/14
CPC分类号： C07K17/14 , C07K7/06 , C07K7/08 , C07K14/245 , C07K2319/00 , C07K2319/23 , C12N9/0006 , C12N11/14
摘要： The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride. The invention involves a peptide having an affinity for silicon nitride, the peptide comprising (1-1) a peptide having the amino acid sequence of one of SEQ ID NOS: 1, 2 and 23 to 27, (1-2) a peptide that has an affinity for silicon nitride and comprises an amino acid sequence obtained by deleting, adding, and/or substituting one or more amino acids in one of the amino acid sequences indicated in (1-1), or a fragment of one of the peptides.

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