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    • 2. 发明申请
    • PROBE FOR iFRET AND USE THEREOF
    • 探索iFRET和其使用
    • US20140242606A1
    • 2014-08-28
    • US14128535
    • 2012-06-25
    • Sang Jeon ChungJu Hwan KimElena RuchkinaHyo Jin Kang
    • Sang Jeon ChungJu Hwan KimElena RuchkinaHyo Jin Kang
    • G01N33/58G01N33/573
    • G01N33/582G01N21/6428G01N33/542G01N33/573G01N2021/6441G01N2458/00
    • The present invention relates to a probe for iFRET and use thereof. Specifically, the present invention relates to a novel probe for iFRET, a method for preparing the probe for iFRET, a method for searching a target protein-specific binding site or a molecule having the binding site using the probe for iFRET, and a method for imaging the target protein using the probe for iFRET. The probe for iFRET according to the present invention utilizes an amino acid in a protein as a fluorescent donor, unlike the conventional FRET method. Therefore, only one fluorescent material is used, and its emission wavelength is distinct from the intrinsic fluorescence of the protein. Thus, high specificity and sensitivity are ensured, and the quantity, activity and mechanism of various proteins can be analyzed in an easy and accurate manner.
    • 本发明涉及一种用于iFRET的探针及其用途。 具体地说,本发明涉及用于iFRET的新型探针,用于制备iFRET探针的方法,使用所述iFRET探针来搜索靶蛋白特异性结合位点的方法或具有结合位点的分子,以及用于 使用iFRET的探针成像靶蛋白。 根据本发明的iFRET的探针利用蛋白质中的氨基酸作为荧光供体,与常规的FRET方法不同。 因此,仅使用一种荧光材料,其发射波长与蛋白质的固有荧光不同。 因此,确保了高度的特异性和灵敏度,可以容易且准确地分析各种蛋白质的数量,活性和机理。
    • 3. 发明申请
    • MICROSCOPE APPARATUS FOR DETECTING OR IMAGING PROTEIN USING PROBE FOR INTRINSIC FLUORESCENCE RESONANCE ENERGY TRANSFER AND METHOD FOR DETECTING OR IMAGING PROTEIN USING THE SAME
    • 用于检测或成像蛋白质的微型装置用于内在荧光共振能量转移和使用其检测或成像蛋白质的方法
    • US20140170769A1
    • 2014-06-19
    • US14128560
    • 2012-04-23
    • Sang Jeon ChungHyo Jin KangJu Hwan Kim
    • Sang Jeon ChungHyo Jin KangJu Hwan Kim
    • G01N21/64
    • G01N21/6428G01N21/6458G01N33/542G01N33/6803G01N2021/6441
    • A microscope apparatus for detecting or imaging a target protein using a probe for intrinsic fluorescence resonance energy transfer (iFRET) according to the present invention comprises: a light irradiation unit that irradiates a first light having a wavelength range for exciting an amino acid in the target protein and a second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET; an objective lens that allows the lights irradiated from the light irradiation unit to be incident onto a sample into which the probe for iFRET is introduced; and a recognition unit that detects a first light emitting signal generated from the probe for iFRET by irradiating the first light having a wavelength range for exciting an amino acid in the target protein onto the sample and a second light emitting signal generated from the probe for iFRET by irradiating the second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET onto the sample, wherein the probe for iFRET includes: a binding site specific to a target protein or a molecule which has the binding site; and a fluorescent molecule having an acceptor function with respect to intrinsic fluorescence of the target protein, which are bonded to each other directly or by a linker.
    • 根据本发明的用于使用本征荧光共振能量转移(iFRET)的探针检测或成像目标蛋白质的显微镜装置包括:照射具有用于激发靶中的氨基酸的波长范围的第一光的光照射单元 蛋白质和具有用于激发用于iFRET的探针的荧光分子的波长范围的第二光; 允许从光照射单元照射的光入射到其中引入用于iFRET的探针的样品的物镜; 以及识别单元,通过将具有用于激发目标蛋白质中的氨基酸的波长范围的第一光照射到样品上,并且从用于iFRET的探针产生的第二发光信号,检测从探针产生的用于iFRET的第一发光信号 通过将具有用于激发iFRET探针的荧光分子的波长范围的第二光照射在样品上,其中所述iFRET探针包括:对靶蛋白或具有结合位点的分子特异性的结合位点; 以及相对于靶蛋白的固有荧光具有受体功能的荧光分子,它们直接或通过连接体彼此结合。