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1. 发明申请

US20140242606A1 PROBE FOR iFRET AND USE THEREOF 审中-公开
标题翻译：探索iFRET和其使用
公开(公告)号：US20140242606A1
公开(公告)日：2014-08-28
申请号：US14128535
申请日：2012-06-25
申请人： Sang Jeon Chung , Ju Hwan Kim , Elena Ruchkina , Hyo Jin Kang
发明人： Sang Jeon Chung , Ju Hwan Kim , Elena Ruchkina , Hyo Jin Kang
IPC分类号： G01N33/58 , G01N33/573
CPC分类号： G01N33/582 , G01N21/6428 , G01N33/542 , G01N33/573 , G01N2021/6441 , G01N2458/00
摘要： The present invention relates to a probe for iFRET and use thereof. Specifically, the present invention relates to a novel probe for iFRET, a method for preparing the probe for iFRET, a method for searching a target protein-specific binding site or a molecule having the binding site using the probe for iFRET, and a method for imaging the target protein using the probe for iFRET. The probe for iFRET according to the present invention utilizes an amino acid in a protein as a fluorescent donor, unlike the conventional FRET method. Therefore, only one fluorescent material is used, and its emission wavelength is distinct from the intrinsic fluorescence of the protein. Thus, high specificity and sensitivity are ensured, and the quantity, activity and mechanism of various proteins can be analyzed in an easy and accurate manner.
摘要翻译：本发明涉及一种用于iFRET的探针及其用途。具体地说，本发明涉及用于iFRET的新型探针，用于制备iFRET探针的方法，使用所述iFRET探针来搜索靶蛋白特异性结合位点的方法或具有结合位点的分子，以及用于使用iFRET的探针成像靶蛋白。根据本发明的iFRET的探针利用蛋白质中的氨基酸作为荧光供体，与常规的FRET方法不同。因此，仅使用一种荧光材料，其发射波长与蛋白质的固有荧光不同。因此，确保了高度的特异性和灵敏度，可以容易且准确地分析各种蛋白质的数量，活性和机理。

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