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1. 发明授权

US4834854A Method of making an electrophoresis medium 失效
标题翻译：制作电泳介质的方法
公开(公告)号：US4834854A
公开(公告)日：1989-05-30
申请号：US234470
申请日：1988-08-19
申请人： Mitsuru Sugihara , Mineo Suyefuji
发明人： Mitsuru Sugihara , Mineo Suyefuji
IPC分类号： G01N27/447
CPC分类号： G01N27/44747
摘要： A method of making an electrophoresis medium having a predetermined gradient in concentrations of an acrylamide compound and a cross-linking agent comprises the steps of mixing two kinds of aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations such that a mixing ratio of the aqueous solutions to each other is changed gradually, thereby to form a predetermined gradual change (gradient) in concentrations of the acrylamide compound and the cross-linking agent in the mixture, and carrying out cross-linking polymerization of the mixture in the presence of a polymerization initiator. Viscosity values of the two kinds of the aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations prior to the mixing are adjusted to be substantially equal to each other.
摘要翻译：制备具有丙烯酰胺化合物和交联剂浓度的预定梯度的电泳介质的方法包括以下步骤：将含有丙烯酰胺化合物和交联剂的两种水溶液以不同浓度混合，使得混合水溶液彼此的比例逐渐变化，从而在混合物中形成丙烯酰胺化合物和交联剂的浓度的预定的逐渐变化（梯度），并进行混合物的交联聚合存在聚合引发剂。将混合前的含有丙烯酰胺化合物和不同浓度的交联剂的两种水溶液的粘度值调整为基本相等。

2. 发明授权

US4968535A Method of producing gradient gel medium membrane for electrophoresis 失效
标题翻译：梯度凝胶介质膜电泳方法
公开(公告)号：US4968535A
公开(公告)日：1990-11-06
申请号：US199726
申请日：1988-05-27
申请人： Fumitaka Terai , Kimio Yukawa , Mineo Suyefuji , Hiroki Saito , Shigeru Yamaguchi
发明人： Fumitaka Terai , Kimio Yukawa , Mineo Suyefuji , Hiroki Saito , Shigeru Yamaguchi
IPC分类号： B05D1/26 , G01N27/447
CPC分类号： B05D1/26 , G01N27/44704 , G01N27/44747
摘要： A method for producing gradient gel medium membrane for electrophoresis in which a monomer solution having a continuous concentration gradient and a polymerizing reaction initiator solution are supplied to a coating head as a gel forming solution for electrophoresis while mixing the monomer solution and the polymerizing reaction initiator solution with each other so as to coat a moving web with the gel forming solution. The coating liquid is supplied onto a moving web through the coating head in such a manner so that the flow of the coating liquid extends continuously in the direction of width and is continuously reduced in the direction of thickness along the coating head from its supply inlet to its slot opening.
摘要翻译：将具有连续浓度梯度的单体溶液和聚合反应引发剂溶液的梯度凝胶介质膜的制造方法作为电泳用凝胶形成溶液供给到涂布头，同时将单体溶液和聚合反应引发剂溶液彼此之间，以便用凝胶形成溶液涂覆移动的纤维网。涂布液以这样的方式供给到移动的幅材上，使得涂布液的流动沿着宽度方向连续延伸，并且沿涂层头的厚度方向从其供应入口到另一个方向连续地减小到其槽口开口。

3. 发明授权

US4699680A Process and apparatus for manufacturing element for electrophoresis 失效
标题翻译：制造电泳元件的方法和装置
公开(公告)号：US4699680A
公开(公告)日：1987-10-13
申请号：US716952
申请日：1985-03-28
申请人： Hisashi Shiraishi , Mineo Suyefuji , Masashi Kato
发明人： Hisashi Shiraishi , Mineo Suyefuji , Masashi Kato
IPC分类号： G01N27/26 , G01N27/447 , B29C39/00 , B32B31/18
CPC分类号： G01N27/44704 , Y10T156/108 , Y10T156/12
摘要： A process of manufacturing an element for electrophotresis which comprises steps of: sticking a spacer continuously to both side portions of a support web which is rolled back continuously from the rolled condition; casting on said support a solution for forming a medium membrane for electrophoresis; subjecting the support to hardening treatment to form a medium membrane; cutting off a portion of the medium membrane and support web; and sticking a cover sheet onto the medium membrane. An apparatus advantageously employable for performing the above process is disclosed.
摘要翻译：一种用于电泳的元件的制造方法，包括以下步骤：将间隔物连续地粘贴到从轧制状态连续回退的支撑纤维网的两侧部分; 在所述载体上浇铸形成用于电泳的介质膜的溶液; 对载体进行硬化处理以形成介质膜; 切断介质膜的一部分和支撑纤维网; 并将覆盖片粘贴到介质膜上。公开了一种有利地用于执行上述过程的设备。

4. 发明授权

US5004628A Coating method and apparatus 失效
标题翻译：涂布方法和装置
公开(公告)号：US5004628A
公开(公告)日：1991-04-02
申请号：US470399
申请日：1990-01-26
申请人： Fumitaka Terai , Kimio Yukawa , Mineo Suyefuji , Hiroki Saito , Shigeru Yamaguchi
发明人： Fumitaka Terai , Kimio Yukawa , Mineo Suyefuji , Hiroki Saito , Shigeru Yamaguchi
IPC分类号： B05C5/00 , B05C5/02 , B05C11/04 , B05D1/26 , D21H23/46 , G03C1/74 , G11B5/848
CPC分类号： B05D1/26 , B05C5/0254 , D21H23/46 , G03C1/74 , G11B5/848 , G03C2001/7459 , G03C2001/7466
摘要： In a coating method using a coating head having a coating solution supplying inlet and a slot opening, the coating solution is caused to flow from the inlet to the slot opening in such a manner that the flow width increases gradually while the flow thickness decreases gradually. An apparatus according to the invention has a coating head in which a flow path is formed between an inlet and a slot opening, such that the flow width gradually increases while the flow thickness gradually decreases.
摘要翻译：在使用具有涂布溶液供给口和槽口的涂布头的涂布方法中，使涂布液从入口流到槽口，流动宽度逐渐增加，同时流动厚度逐渐减小。根据本发明的装置具有涂层头，其中在入口和狭槽开口之间形成流动通道，使得流动宽度逐渐增加，同时流动厚度逐渐减小。

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