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1. 发明授权

US4844786A Means for electrophoresis 失效
标题翻译：电泳方法
公开(公告)号：US4844786A
公开(公告)日：1989-07-04
申请号：US160161
申请日：1988-02-25
申请人： Mitsuru Sugihara , Masashi Ogawa
发明人： Mitsuru Sugihara , Masashi Ogawa
IPC分类号： G01N27/447
CPC分类号： G01N27/44704 , G01N27/44747
摘要： A means for electrophoresis suitably employable for analysis of biopolymers such as proteins, as well as for determination of base sequence of DNA, RNA, their fragments, and their derivatives is disclosed. The means comprises a membrane of an aqueous polyacrylamide gel for electrophoresis which is sandwiched between a plane support and a plane covering sheet. The membrane has a thickness gradient in its electrophoresis direction.

2. 发明授权

US5190629A Electrophoresis medium membrane 失效
标题翻译：电泳介质膜
公开(公告)号：US5190629A
公开(公告)日：1993-03-02
申请号：US604358
申请日：1990-10-26
申请人： Mitsuru Sugihara , Masashi Ogawa
发明人： Mitsuru Sugihara , Masashi Ogawa
IPC分类号： G01N27/447
CPC分类号： G01N27/44747 , G01N27/44704
摘要： An electrophoresis medium membrane comprises a planar support, a planar cover sheet, and a layer of an electrophoresis gel medium provided between the planar support and the planar cover sheet. The electrophoresis gel medium contains an aqueous polyacrylamide gel, which is prepared by cross-linking polymerization of an acrylamide compound and a cross-linking agent in the presence of water and a compound having at least one carbamoyl group as a denaturing agent (or modifier). The layer of the electrophoresis gel medium has both a predetermined gradual change (gradient) in layer thickness and a predetermined gradual change (gradient) in concentrations of the acrylamide compound and the cross-linking agent.
摘要翻译：电泳介质膜包括平面支撑体，平面覆盖片和设置在平面支撑体和平面覆盖片之间的电泳凝胶介质层。电泳凝胶介质含有水性聚丙烯酰胺凝胶，其通过在水和具有至少一个氨基甲酰基的化合物作为变性剂（或改性剂）的存在下交联丙烯酰胺化合物和交联剂的聚合制备，。电泳凝胶介质层在丙烯酰胺化合物和交联剂的浓度上具有预定的层厚度逐渐变化（梯度）和预定的逐渐变化（梯度）。

3. 发明授权

US4834854A Method of making an electrophoresis medium 失效
标题翻译：制作电泳介质的方法
公开(公告)号：US4834854A
公开(公告)日：1989-05-30
申请号：US234470
申请日：1988-08-19
申请人： Mitsuru Sugihara , Mineo Suyefuji
发明人： Mitsuru Sugihara , Mineo Suyefuji
IPC分类号： G01N27/447
CPC分类号： G01N27/44747
摘要： A method of making an electrophoresis medium having a predetermined gradient in concentrations of an acrylamide compound and a cross-linking agent comprises the steps of mixing two kinds of aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations such that a mixing ratio of the aqueous solutions to each other is changed gradually, thereby to form a predetermined gradual change (gradient) in concentrations of the acrylamide compound and the cross-linking agent in the mixture, and carrying out cross-linking polymerization of the mixture in the presence of a polymerization initiator. Viscosity values of the two kinds of the aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations prior to the mixing are adjusted to be substantially equal to each other.
摘要翻译：制备具有丙烯酰胺化合物和交联剂浓度的预定梯度的电泳介质的方法包括以下步骤：将含有丙烯酰胺化合物和交联剂的两种水溶液以不同浓度混合，使得混合水溶液彼此的比例逐渐变化，从而在混合物中形成丙烯酰胺化合物和交联剂的浓度的预定的逐渐变化（梯度），并进行混合物的交联聚合存在聚合引发剂。将混合前的含有丙烯酰胺化合物和不同浓度的交联剂的两种水溶液的粘度值调整为基本相等。

4. 发明授权

US08567234B2 Gas sensor 有权
标题翻译：气体传感器
公开(公告)号：US08567234B2
公开(公告)日：2013-10-29
申请号：US13076723
申请日：2011-03-31
申请人： Yasuhiro Fujita , Mitsuru Sugihara , Chihiro Seki , Takayoshi Atsumi
发明人： Yasuhiro Fujita , Mitsuru Sugihara , Chihiro Seki , Takayoshi Atsumi
IPC分类号： G01N9/00
CPC分类号： G01N27/4077
摘要： A gas sensor, including a cylindrical outer tube; and a seal member disposed therein, the seal member including: a lead wire insert hole and an atmosphere communication hole. The seal member has a groove extending outwards in a radial direction at a rear end-facing surface from the atmosphere communication hole while circumventing the lead wire insert hole, the groove having a cutout toward the front end of the seal member. The gas sensor further includes: a protection portion which covers the atmosphere communication hole; and an arm couples the protection portion and the outer tube, and extends in the radial direction, the arm being at least partially disposed in the groove of the seal member. and the arm is disposed so as to define a gap with the bottom surface of the groove.
摘要翻译：气体传感器，包括圆柱形外管; 以及设置在其中的密封构件，所述密封构件包括：引线插入孔和大气连通孔。密封构件具有从与大气连通孔的后端面向径向向外延伸的槽，同时避开引线插入孔，该沟槽具有朝向密封构件前端的切口。气体传感器还包括：覆盖大气连通孔的保护部分; 并且臂连接保护部分和外管，并且在径向方向上延伸，臂至少部分地设置在密封构件的凹槽中。臂设置成与槽的底面形成间隙。

5. 发明授权

US4695354A Medium for electrophoresis 失效
标题翻译：电泳培养基
公开(公告)号：US4695354A
公开(公告)日：1987-09-22
申请号：US908376
申请日：1986-09-17
申请人： Mitsuru Sugihara , Naohiko Sugimoto
发明人： Mitsuru Sugihara , Naohiko Sugimoto
IPC分类号： B01D57/02 , C08F220/56 , G01N27/447 , G01N27/26 , C08K5/21
CPC分类号： G01N27/44747 , C08F220/56
摘要： In a medium for electrophoresis suitably employable for determination of base sequence of DNA, RNA, their fragments, and their derivatives, which comprises an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water and at least one compound having a carbamoyl group, the improvement wherein glycerol is contained in the medium in a small amount ranging from 0.1 to 1.0 wt/v %.
摘要翻译：在适用于测定DNA，RNA及其片段及其衍生物的碱基序列的电泳介质中，其包含通过丙烯酰胺化合物和交联剂在水存在下交联聚合而形成的水性聚丙烯酰胺凝胶，并且至少一种具有氨基甲酰基的化合物，其改进在于介质中含有0.1至1.0重量/体积％的少量甘油。

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